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The map label for this gene is greB [H]

Identifier: 114331547

GI number: 114331547

Start: 1633040

End: 1633588

Strand: Direct

Name: greB [H]

Synonym: Neut_1560

Alternate gene names: 114331547

Gene position: 1633040-1633588 (Clockwise)

Preceding gene: 114331545

Following gene: 114331548

Centisome position: 61.37

GC content: 49.91

Gene sequence:

>549_bases
GTGAATAAGGCTTTTACTAAAGAGAGTGACCACGAGGAAGAGCTGGATGACACCCCGCAACTGCCGCAGGGCGTAAAGAA
TTATATTACCCCGGGAGGCTATCAGCGATTGAAGGATGAATTTGATCAGTTATGGAAAGTGGAGCGCCCGGAACTGGTCA
AGATTGTATCCTGGGCAGCATCAAACGGTGATCGCTCAGAAAATGGTGATTATATTTATGGCAAGCGGCGCTTGCGTGAA
ATTGATCGCCGTTTGCGGTTTTTATCCAGGCGCATGGACCATGCCGAAATCATCGACCCGGCACAACGCGGCGAGTGCGA
TCAGGTATTTTTTGGTGCGACAGTAACGGTATACGACGCACAAGGCAAAGAGCAGACATACAGTATTGTCGGTATGGATG
AAGCGGAGCCCAGCTGTGGCCGTATCAGCTGGATTTCACCGCTGGCCAGAGCACTACTGAAAGCACGAGAGGGGGATGTG
GTGTCTTTGCAAACACCAGGTGGCCTGGAAGAACTGGAAGTAATTAAAATCCGTTATACGATATTGTGA

Upstream 100 bases:

>100_bases
TAATGATGACTTGCATGGCTTTGAGTATAACAGGCAGATTAATTTGAAAGTGTCTGCAAAAGCAGTATTTGTTTATACAA
AATGATGGGGGAATTGAATA

Downstream 100 bases:

>100_bases
TACAGACTCTCTGATCAGCTATCCCATCATTCATACACCGGCTTCCTTACGATCCGTCTGTACTACGCCCGGCATAATAC
CTGGAGCTTCAACACCGTAC

Product: transcription elongation factor GreB

Products: NA

Alternate protein names: Transcript cleavage factor greB [H]

Number of amino acids: Translated: 182; Mature: 182

Protein sequence:

>182_residues
MNKAFTKESDHEEELDDTPQLPQGVKNYITPGGYQRLKDEFDQLWKVERPELVKIVSWAASNGDRSENGDYIYGKRRLRE
IDRRLRFLSRRMDHAEIIDPAQRGECDQVFFGATVTVYDAQGKEQTYSIVGMDEAEPSCGRISWISPLARALLKAREGDV
VSLQTPGGLEELEVIKIRYTIL

Sequences:

>Translated_182_residues
MNKAFTKESDHEEELDDTPQLPQGVKNYITPGGYQRLKDEFDQLWKVERPELVKIVSWAASNGDRSENGDYIYGKRRLRE
IDRRLRFLSRRMDHAEIIDPAQRGECDQVFFGATVTVYDAQGKEQTYSIVGMDEAEPSCGRISWISPLARALLKAREGDV
VSLQTPGGLEELEVIKIRYTIL
>Mature_182_residues
MNKAFTKESDHEEELDDTPQLPQGVKNYITPGGYQRLKDEFDQLWKVERPELVKIVSWAASNGDRSENGDYIYGKRRLRE
IDRRLRFLSRRMDHAEIIDPAQRGECDQVFFGATVTVYDAQGKEQTYSIVGMDEAEPSCGRISWISPLARALLKAREGDV
VSLQTPGGLEELEVIKIRYTIL

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082261, Length=154, Percent_Identity=47.4025974025974, Blast_Score=132, Evalue=1e-32,
Organism=Escherichia coli, GI87082225, Length=152, Percent_Identity=38.8157894736842, Blast_Score=104, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR001437
- InterPro:   IPR022691
- InterPro:   IPR006358 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 20838; Mature: 20838

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKAFTKESDHEEELDDTPQLPQGVKNYITPGGYQRLKDEFDQLWKVERPELVKIVSWAA
CCCCCCCCCCCHHHCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHC
SNGDRSENGDYIYGKRRLREIDRRLRFLSRRMDHAEIIDPAQRGECDQVFFGATVTVYDA
CCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCHHHHCCCHHCCCCCEEEECCEEEEEEC
QGKEQTYSIVGMDEAEPSCGRISWISPLARALLKAREGDVVSLQTPGGLEELEVIKIRYT
CCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEEEE
IL
EC
>Mature Secondary Structure
MNKAFTKESDHEEELDDTPQLPQGVKNYITPGGYQRLKDEFDQLWKVERPELVKIVSWAA
CCCCCCCCCCCHHHCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHC
SNGDRSENGDYIYGKRRLREIDRRLRFLSRRMDHAEIIDPAQRGECDQVFFGATVTVYDA
CCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCHHHHCCCHHCCCCCEEEECCEEEEEEC
QGKEQTYSIVGMDEAEPSCGRISWISPLARALLKAREGDVVSLQTPGGLEELEVIKIRYT
CCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEEEE
IL
EC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]