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The map label for this gene is gloA [H]

Identifier: 114331528

GI number: 114331528

Start: 1610091

End: 1610480

Strand: Direct

Name: gloA [H]

Synonym: Neut_1541

Alternate gene names: 114331528

Gene position: 1610091-1610480 (Clockwise)

Preceding gene: 114331527

Following gene: 114331529

Centisome position: 60.51

GC content: 45.38

Gene sequence:

>390_bases
ATGCGCATTCTTCATACCATGTTGAGAGTAGGTAATCTGGAGCGTTCGATCCAGTTTTACACCGATGTCCTCGGAATGCA
AATTCTGCGCCGTAAGGATTACCCAGAAGGAAAGTTCACATTGGCATTCGTCGGTTATCAAAGTGAAGCGGAAGGTACCG
TTCTGGAGCTGACGCATAATTGGGAAATCGACCATTATGATCTCGGAACAGGTTTTGGACACATTGCAATCGAAGTAGAC
AATGCCTACGAAGCCTGTGAAAAGGTCAGAAACCTGGGAGGTCGCGTCACGCGAGAGGCTGGACCGATGAAACATGGAAC
TACCGTGATTGCTTTCATAGAAGATCCGGATGGTTACAAGATTGAATTTATTCAAAAGAAAACAGTGTAA

Upstream 100 bases:

>100_bases
ATGGTATTCCTGCCGGAACCCGCTGGGAAGATATTCCCATGAACTGGACTTGCCCGGAATGTGGTGCACGAAAAGAAGAT
TTTGAAATGGTGAAAATATA

Downstream 100 bases:

>100_bases
CTGATGTTGCCATGGGGAGCTTGGCTGTAGCATAGTGCTCAGCCATTCAAAACGATACTTACAGTCTATGCTGAACATAA
TCCTCAGTTTGCTGTCTGGC

Product: lactoylglutathione lyase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 129; Mature: 129

Protein sequence:

>129_residues
MRILHTMLRVGNLERSIQFYTDVLGMQILRRKDYPEGKFTLAFVGYQSEAEGTVLELTHNWEIDHYDLGTGFGHIAIEVD
NAYEACEKVRNLGGRVTREAGPMKHGTTVIAFIEDPDGYKIEFIQKKTV

Sequences:

>Translated_129_residues
MRILHTMLRVGNLERSIQFYTDVLGMQILRRKDYPEGKFTLAFVGYQSEAEGTVLELTHNWEIDHYDLGTGFGHIAIEVD
NAYEACEKVRNLGGRVTREAGPMKHGTTVIAFIEDPDGYKIEFIQKKTV
>Mature_129_residues
MRILHTMLRVGNLERSIQFYTDVLGMQILRRKDYPEGKFTLAFVGYQSEAEGTVLELTHNWEIDHYDLGTGFGHIAIEVD
NAYEACEKVRNLGGRVTREAGPMKHGTTVIAFIEDPDGYKIEFIQKKTV

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=149, Percent_Identity=36.9127516778523, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1787940, Length=127, Percent_Identity=68.503937007874, Blast_Score=189, Evalue=7e-50,
Organism=Caenorhabditis elegans, GI17552228, Length=133, Percent_Identity=30.8270676691729, Blast_Score=71, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323639, Length=139, Percent_Identity=38.1294964028777, Blast_Score=91, Evalue=4e-20,
Organism=Drosophila melanogaster, GI20129743, Length=151, Percent_Identity=36.4238410596026, Blast_Score=90, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 14722; Mature: 14722

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS00934 GLYOXALASE_I_1 ; PS00935 GLYOXALASE_I_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILHTMLRVGNLERSIQFYTDVLGMQILRRKDYPEGKFTLAFVGYQSEAEGTVLELTHN
CHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCEEEEEEEC
WEIDHYDLGTGFGHIAIEVDNAYEACEKVRNLGGRVTREAGPMKHGTTVIAFIEDPDGYK
CCCCEEECCCCCEEEEEEECCHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEEECCCCCE
IEFIQKKTV
EEEEEECCC
>Mature Secondary Structure
MRILHTMLRVGNLERSIQFYTDVLGMQILRRKDYPEGKFTLAFVGYQSEAEGTVLELTHN
CHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCEEEEEEEC
WEIDHYDLGTGFGHIAIEVDNAYEACEKVRNLGGRVTREAGPMKHGTTVIAFIEDPDGYK
CCCCEEECCCCCEEEEEEECCHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEEECCCCCE
IEFIQKKTV
EEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]