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The map label for this gene is hemL

Identifier: 114331524

GI number: 114331524

Start: 1607057

End: 1608340

Strand: Reverse

Name: hemL

Synonym: Neut_1537

Alternate gene names: 114331524

Gene position: 1608340-1607057 (Counterclockwise)

Preceding gene: 114331525

Following gene: 114331523

Centisome position: 60.44

GC content: 49.3

Gene sequence:

>1284_bases
ATGACGATCACTAATCAACAATTGTTCGAGCGCTCCAGGCAATACATTCCGGGAGGCGTTAATTCACCAGTACGTGCTTT
TAAATCCGTAGGAGGTACGCCTATTTTTTTTCAGCGCGGGCAGGGTGCTTGTTTCTGGGATGTGGAAGGAAAATCCTATA
TTGATTACGTTGGATCATGGGGGCCGCTTATTCTCGGGCATGCGCACCCCGATGTTGTCAGGGCCGTTCAGGATGCGGCA
AGCCGTGGTACCTCTTTTGGTGCTCCAACGGCAGCAGAGCTGGAAATAGCCGAATTGTTATGTCGGTTGTTGCCTTCCCT
GGAACTGGTACGGTTGGTAAGTTCCGGCACAGAGGCTGGAATGAGTGCCATTCGGCTCGCGCGTGGTTATACCGGCAGAA
ACCGGATTATCAAGTTTGAAGGTTGCTATCACGGGCACGATGATGCCTTGCTGGTTAAAGCAGGTTCAGGCGCATTGACT
TTCGGCCATCCCAGTTCAGCAGGGGTTCCCGCTGAAACAGCAGGAAATACTCTGGTATTGAACTATAACGATGTTGCCGG
AGTGGAAGCAACCTTCAGCAAACTGGGTTCTGAAATTGCCGCTGTGATCGTCGAACCAGTGGCGGGCAATATGAATTTGA
TTAAGGCAACCCCCCAGTTTCTGGAAACATTACGCACACTATGTACAAAGCATAGCAGTTTGCTGATTCTGGATGAGGTG
ATGACAGGTTTCCGCGTTGGACTGGAATGTGCCCAAGGGCTATATGGCATCAGGCCAGATCTAACCATTCTAGGGAAAGT
AATCGGTGGGGGGTTACCCATGGCAGCGTTTGGTGGACGACGTGATGTGATGGAATGCCTTGCGCCATTGGGACCCGTGT
ATCAAGCAGGTACACTTTCCGGCAATCCAGTTGCAGTTGCTGCCGGGTTGGAAACTTTGCGTCAAGTACAGACGCCTGGT
TTTTTTGACAAGTTGTCAATGATGACACGTAAGTTAACAGACGGCCTGACTTCTGTCGCCATAAAAAATGGCGTGGTGTT
TAGTGCACAGGCAGTGGGTGGAATGTTTGGAATTTATTTCAGGAAAACACCGCCAGTAAGTTTTACAGAAATAATGGAGA
GTGACCGTGATGCGTTTAACCGTTTTTTTCATACCATGCTGAAAGAGGGTATTTATTTTGCACCCTCAGCTTTCGAAGCA
GGCTTCGTTTCTGCTGCGCACGGGGATGTGGAAATTGACAAAACACTGGAAGCAGCTGATCGGGTTTTCAGTCAAAAAGC
GTAA

Upstream 100 bases:

>100_bases
GGCGCTGTTCAGCGCACAAGATATCCAATCTGCTGCCCGGCATTTTTCAAAGCTATTCTGTTGAACCATCAATTTCGATA
ACTTTTAAGCAAATATTCCA

Downstream 100 bases:

>100_bases
GAGATACCAAAGAGTGCAATTCACGACATATAAATAAAAGATTCAATTCGTGAGGTTGGGTGCCGTTTATATTTTTCCAA
TCGGCTTGTTGCGATTCTTG

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MTITNQQLFERSRQYIPGGVNSPVRAFKSVGGTPIFFQRGQGACFWDVEGKSYIDYVGSWGPLILGHAHPDVVRAVQDAA
SRGTSFGAPTAAELEIAELLCRLLPSLELVRLVSSGTEAGMSAIRLARGYTGRNRIIKFEGCYHGHDDALLVKAGSGALT
FGHPSSAGVPAETAGNTLVLNYNDVAGVEATFSKLGSEIAAVIVEPVAGNMNLIKATPQFLETLRTLCTKHSSLLILDEV
MTGFRVGLECAQGLYGIRPDLTILGKVIGGGLPMAAFGGRRDVMECLAPLGPVYQAGTLSGNPVAVAAGLETLRQVQTPG
FFDKLSMMTRKLTDGLTSVAIKNGVVFSAQAVGGMFGIYFRKTPPVSFTEIMESDRDAFNRFFHTMLKEGIYFAPSAFEA
GFVSAAHGDVEIDKTLEAADRVFSQKA

Sequences:

>Translated_427_residues
MTITNQQLFERSRQYIPGGVNSPVRAFKSVGGTPIFFQRGQGACFWDVEGKSYIDYVGSWGPLILGHAHPDVVRAVQDAA
SRGTSFGAPTAAELEIAELLCRLLPSLELVRLVSSGTEAGMSAIRLARGYTGRNRIIKFEGCYHGHDDALLVKAGSGALT
FGHPSSAGVPAETAGNTLVLNYNDVAGVEATFSKLGSEIAAVIVEPVAGNMNLIKATPQFLETLRTLCTKHSSLLILDEV
MTGFRVGLECAQGLYGIRPDLTILGKVIGGGLPMAAFGGRRDVMECLAPLGPVYQAGTLSGNPVAVAAGLETLRQVQTPG
FFDKLSMMTRKLTDGLTSVAIKNGVVFSAQAVGGMFGIYFRKTPPVSFTEIMESDRDAFNRFFHTMLKEGIYFAPSAFEA
GFVSAAHGDVEIDKTLEAADRVFSQKA
>Mature_426_residues
TITNQQLFERSRQYIPGGVNSPVRAFKSVGGTPIFFQRGQGACFWDVEGKSYIDYVGSWGPLILGHAHPDVVRAVQDAAS
RGTSFGAPTAAELEIAELLCRLLPSLELVRLVSSGTEAGMSAIRLARGYTGRNRIIKFEGCYHGHDDALLVKAGSGALTF
GHPSSAGVPAETAGNTLVLNYNDVAGVEATFSKLGSEIAAVIVEPVAGNMNLIKATPQFLETLRTLCTKHSSLLILDEVM
TGFRVGLECAQGLYGIRPDLTILGKVIGGGLPMAAFGGRRDVMECLAPLGPVYQAGTLSGNPVAVAAGLETLRQVQTPGF
FDKLSMMTRKLTDGLTSVAIKNGVVFSAQAVGGMFGIYFRKTPPVSFTEIMESDRDAFNRFFHTMLKEGIYFAPSAFEAG
FVSAAHGDVEIDKTLEAADRVFSQKA

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily

Homologues:

Organism=Homo sapiens, GI4557809, Length=348, Percent_Identity=31.0344827586207, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI24119277, Length=342, Percent_Identity=27.4853801169591, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI37574042, Length=354, Percent_Identity=25.7062146892655, Blast_Score=90, Evalue=5e-18,
Organism=Homo sapiens, GI284507298, Length=266, Percent_Identity=30.8270676691729, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI226442705, Length=353, Percent_Identity=24.929178470255, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI226442709, Length=323, Percent_Identity=25.6965944272446, Blast_Score=79, Evalue=6e-15,
Organism=Escherichia coli, GI1786349, Length=422, Percent_Identity=58.5308056872038, Blast_Score=518, Evalue=1e-148,
Organism=Escherichia coli, GI1789016, Length=397, Percent_Identity=33.5012594458438, Blast_Score=182, Evalue=5e-47,
Organism=Escherichia coli, GI1788044, Length=333, Percent_Identity=30.03003003003, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI1789759, Length=329, Percent_Identity=29.7872340425532, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI1787560, Length=374, Percent_Identity=27.807486631016, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI145693181, Length=378, Percent_Identity=28.042328042328, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1786991, Length=372, Percent_Identity=27.1505376344086, Blast_Score=90, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI25144271, Length=355, Percent_Identity=27.3239436619718, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI71992977, Length=389, Percent_Identity=25.706940874036, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI25144274, Length=160, Percent_Identity=29.375, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323470, Length=377, Percent_Identity=25.7294429708223, Blast_Score=117, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6324432, Length=359, Percent_Identity=25.6267409470752, Blast_Score=87, Evalue=5e-18,
Organism=Drosophila melanogaster, GI21357415, Length=318, Percent_Identity=26.7295597484277, Blast_Score=113, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSA_NITEC (Q0AFV1)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_747746.1
- ProteinModelPortal:   Q0AFV1
- SMR:   Q0AFV1
- STRING:   Q0AFV1
- GeneID:   4273410
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_1537
- NMPDR:   fig|335283.3.peg.1819
- eggNOG:   COG0001
- HOGENOM:   HBG725944
- OMA:   VGCFGGK
- PhylomeDB:   Q0AFV1
- ProtClustDB:   PRK00062
- BioCyc:   NEUT335283:NEUT_1537-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00375
- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11986
- TIGRFAMs:   TIGR00713

Pfam domain/function: PF00202 Aminotran_3; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =5.4.3.8

Molecular weight: Translated: 45514; Mature: 45383

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTITNQQLFERSRQYIPGGVNSPVRAFKSVGGTPIFFQRGQGACFWDVEGKSYIDYVGSW
CCCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEEECCCCCEEEECCCCHHHHHHCCC
GPLILGHAHPDVVRAVQDAASRGTSFGAPTAAELEIAELLCRLLPSLELVRLVSSGTEAG
CCEEEECCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHCCCHHH
MSAIRLARGYTGRNRIIKFEGCYHGHDDALLVKAGSGALTFGHPSSAGVPAETAGNTLVL
HHHHHHHHCCCCCCCEEEEECCCCCCCCEEEEEECCCEEEECCCCCCCCCCCCCCCEEEE
NYNDVAGVEATFSKLGSEIAAVIVEPVAGNMNLIKATPQFLETLRTLCTKHSSLLILDEV
ECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHCCCCCEEEHHHH
MTGFRVGLECAQGLYGIRPDLTILGKVIGGGLPMAAFGGRRDVMECLAPLGPVYQAGTLS
HHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHHCCCHHHHHHHHHCCCCCCCCCCCC
GNPVAVAAGLETLRQVQTPGFFDKLSMMTRKLTDGLTSVAIKNGVVFSAQAVGGMFGIYF
CCCEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHCCCEEEEE
RKTPPVSFTEIMESDRDAFNRFFHTMLKEGIYFAPSAFEAGFVSAAHGDVEIDKTLEAAD
ECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCEECCCCCEEHHHHHHHHH
RVFSQKA
HHHHCCC
>Mature Secondary Structure 
TITNQQLFERSRQYIPGGVNSPVRAFKSVGGTPIFFQRGQGACFWDVEGKSYIDYVGSW
CCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEEECCCCCEEEECCCCHHHHHHCCC
GPLILGHAHPDVVRAVQDAASRGTSFGAPTAAELEIAELLCRLLPSLELVRLVSSGTEAG
CCEEEECCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHCCCHHH
MSAIRLARGYTGRNRIIKFEGCYHGHDDALLVKAGSGALTFGHPSSAGVPAETAGNTLVL
HHHHHHHHCCCCCCCEEEEECCCCCCCCEEEEEECCCEEEECCCCCCCCCCCCCCCEEEE
NYNDVAGVEATFSKLGSEIAAVIVEPVAGNMNLIKATPQFLETLRTLCTKHSSLLILDEV
ECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHCCCCCEEEHHHH
MTGFRVGLECAQGLYGIRPDLTILGKVIGGGLPMAAFGGRRDVMECLAPLGPVYQAGTLS
HHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHHCCCHHHHHHHHHCCCCCCCCCCCC
GNPVAVAAGLETLRQVQTPGFFDKLSMMTRKLTDGLTSVAIKNGVVFSAQAVGGMFGIYF
CCCEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHCCCEEEEE
RKTPPVSFTEIMESDRDAFNRFFHTMLKEGIYFAPSAFEAGFVSAAHGDVEIDKTLEAAD
ECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCEECCCCCEEHHHHHHHHH
RVFSQKA
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA