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The map label for this gene is pleD [H]

Identifier: 114331433

GI number: 114331433

Start: 1527376

End: 1529031

Strand: Direct

Name: pleD [H]

Synonym: Neut_1444

Alternate gene names: 114331433

Gene position: 1527376-1529031 (Clockwise)

Preceding gene: 114331432

Following gene: 114331434

Centisome position: 57.4

GC content: 45.53

Gene sequence:

>1656_bases
TTGAGACTGGTTCTGTACAGTATAAAAAGCAAGATTGTAATTTTTGCTGTGATTGCCACTTTGTTGCCTACAATCAGCCT
CAGTCTATTGTCATTCAAGCAGAATGAAGCGCTGATTAGCGACAGCGTTACCCGTGAACTGCGCATACTTGCTGACAACA
CCAATCGCCAGCTGGATCAATGGGCAGAAGAAAATATGCTTGCCATCCGCGCATTATCAACATCTAGCCCCGTTATTGAG
GGATTGACTACTTTAAAACAGTTTTCAGATAGCAAGAGTGCTAGTGCCGTAAAGCAGACGCAATCTGTCATGGCCAGTTA
TTTGCGCTCGGTACAGATAAAGCTAGATGAAGTACTTGAATTAACTGTTTTTGATGCCACCCGAAAAATTGTGGCAAGCA
GTAGTACTAGTACGCCTGAGGTACGCGAACCCCCTGAACAATGGACGCATACCTCCCTTACTTCGGGAACCATTGCCATC
CCTCCCGCCTGGAACGATCGTTTTGCTACAGCAACTATTAGTATTATTTATCCAGTGCTGTCGTATGACAGCCAGTTAAT
AGGCGCTATCGCTGCCACTCTGGATTTGGGAACCTTCGAAAGCAGATTGGCTGAAATCAAGAAATTTTCGTCAGGTGAAA
TTGTCCTACTGGATCAGCATGGCAAGGTATTGCTGGGTAGCGCCCCCGGCGTTGATCACCAGGCAGTATTAAGCCCGCAT
CAATGGGAATTAATTCAGATGTTCGATGAACCAATATCCTATGATGGATTATCTTATCCAAAAGCAATCGGCTTGGCCCA
TACATCAGAAAATATTCCCGTAACGATCCTGGTAGAGCAGGATCACAGTGCCATACAAGCTGCATGGATAAAGTTACGTA
ATCGTTTTCTTGAGTTTGTCAGCATACTTATCATTATTGTAACCGCCGTAGCCTTGTATATGGGACATTCTATTGTTACA
CCGCTGAAACAGCTGATCAACGCAGTAAAAGGGATCGTAGAAGGAAATTTGGATATTTATCTGCCAGCCAAGCGAAGAGA
TGAAGTTGGGCAGCTTACTGATATATTCAATCAAATGACGAGGGCATTACGCAACAAGCATATAGAAATCATGACTGCCA
ATCAGGCTATGCAGCAACAAAACCAACTACTGCAAAAACTCTCAATCACGGATGGTCTGACGGGCCTGTATAACCGCACA
AAACTCGATACCATCCTGTCAGAACAGCTAGCCAGATTCAAACGCCACGACCGCCCCTTCTGCCTGCTGATGATAGACAT
TGATTATTTCAAAACCATAAATGATAAACTTGGCCATATTGTGGGCGACAGGATTTTGGTCACGGTAGCTTCCGTCCTGC
TGAAATCGATCAGAACAATAGACTATGCCGCACGTTATGGGGGGGATGAATTCGTGGTTATTCTGACTGAAACTAACTCC
AGTGCAGCGATCAAAACAGCAGAACGGATCCGGGCCGAAGTCAACTCTGCCTGCCTTGCATTCAAGGAGCATCCAGCCCA
CATTACTCTGAGTATCGGTATTACCCAAAGTTACTACGATGATATAACGCCGAATGATCTGATTGCTCGCGCAGATGCGG
CGCTGTATGAAGCCAAAGAATCCGGGCGTGACCGGGTACACTGTGCTGATGCATAA

Upstream 100 bases:

>100_bases
TCCGAGTAAATGACGATACTGCTGTAATTGGCATGAAATGCTGGCTGTAATAAATATCAAGCAATACTATTTTTATCACT
TTATGATGGTTTAACTCATT

Downstream 100 bases:

>100_bases
CACTCTAGCCGGCTTCAACATAACTTACTCCATCCGGACACAATAGAAATGGAATATAAGGATTATTATCAAATTATGGG
CATTCCGCGCGATGCGACAC

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 551; Mature: 551

Protein sequence:

>551_residues
MRLVLYSIKSKIVIFAVIATLLPTISLSLLSFKQNEALISDSVTRELRILADNTNRQLDQWAEENMLAIRALSTSSPVIE
GLTTLKQFSDSKSASAVKQTQSVMASYLRSVQIKLDEVLELTVFDATRKIVASSSTSTPEVREPPEQWTHTSLTSGTIAI
PPAWNDRFATATISIIYPVLSYDSQLIGAIAATLDLGTFESRLAEIKKFSSGEIVLLDQHGKVLLGSAPGVDHQAVLSPH
QWELIQMFDEPISYDGLSYPKAIGLAHTSENIPVTILVEQDHSAIQAAWIKLRNRFLEFVSILIIIVTAVALYMGHSIVT
PLKQLINAVKGIVEGNLDIYLPAKRRDEVGQLTDIFNQMTRALRNKHIEIMTANQAMQQQNQLLQKLSITDGLTGLYNRT
KLDTILSEQLARFKRHDRPFCLLMIDIDYFKTINDKLGHIVGDRILVTVASVLLKSIRTIDYAARYGGDEFVVILTETNS
SAAIKTAERIRAEVNSACLAFKEHPAHITLSIGITQSYYDDITPNDLIARADAALYEAKESGRDRVHCADA

Sequences:

>Translated_551_residues
MRLVLYSIKSKIVIFAVIATLLPTISLSLLSFKQNEALISDSVTRELRILADNTNRQLDQWAEENMLAIRALSTSSPVIE
GLTTLKQFSDSKSASAVKQTQSVMASYLRSVQIKLDEVLELTVFDATRKIVASSSTSTPEVREPPEQWTHTSLTSGTIAI
PPAWNDRFATATISIIYPVLSYDSQLIGAIAATLDLGTFESRLAEIKKFSSGEIVLLDQHGKVLLGSAPGVDHQAVLSPH
QWELIQMFDEPISYDGLSYPKAIGLAHTSENIPVTILVEQDHSAIQAAWIKLRNRFLEFVSILIIIVTAVALYMGHSIVT
PLKQLINAVKGIVEGNLDIYLPAKRRDEVGQLTDIFNQMTRALRNKHIEIMTANQAMQQQNQLLQKLSITDGLTGLYNRT
KLDTILSEQLARFKRHDRPFCLLMIDIDYFKTINDKLGHIVGDRILVTVASVLLKSIRTIDYAARYGGDEFVVILTETNS
SAAIKTAERIRAEVNSACLAFKEHPAHITLSIGITQSYYDDITPNDLIARADAALYEAKESGRDRVHCADA
>Mature_551_residues
MRLVLYSIKSKIVIFAVIATLLPTISLSLLSFKQNEALISDSVTRELRILADNTNRQLDQWAEENMLAIRALSTSSPVIE
GLTTLKQFSDSKSASAVKQTQSVMASYLRSVQIKLDEVLELTVFDATRKIVASSSTSTPEVREPPEQWTHTSLTSGTIAI
PPAWNDRFATATISIIYPVLSYDSQLIGAIAATLDLGTFESRLAEIKKFSSGEIVLLDQHGKVLLGSAPGVDHQAVLSPH
QWELIQMFDEPISYDGLSYPKAIGLAHTSENIPVTILVEQDHSAIQAAWIKLRNRFLEFVSILIIIVTAVALYMGHSIVT
PLKQLINAVKGIVEGNLDIYLPAKRRDEVGQLTDIFNQMTRALRNKHIEIMTANQAMQQQNQLLQKLSITDGLTGLYNRT
KLDTILSEQLARFKRHDRPFCLLMIDIDYFKTINDKLGHIVGDRILVTVASVLLKSIRTIDYAARYGGDEFVVILTETNS
SAAIKTAERIRAEVNSACLAFKEHPAHITLSIGITQSYYDDITPNDLIARADAALYEAKESGRDRVHCADA

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=226, Percent_Identity=35.3982300884956, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1787262, Length=163, Percent_Identity=40.4907975460123, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI1786584, Length=179, Percent_Identity=36.3128491620112, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI87082007, Length=185, Percent_Identity=37.2972972972973, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1787816, Length=167, Percent_Identity=36.5269461077844, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788085, Length=171, Percent_Identity=37.4269005847953, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1788381, Length=175, Percent_Identity=33.1428571428571, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1787802, Length=174, Percent_Identity=32.7586206896552, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI87081881, Length=176, Percent_Identity=34.6590909090909, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI87081974, Length=167, Percent_Identity=34.1317365269461, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1788956, Length=305, Percent_Identity=25.2459016393443, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1787541, Length=162, Percent_Identity=32.0987654320988, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI87081977, Length=192, Percent_Identity=29.1666666666667, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 61103; Mature: 61103

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS50885 HAMP ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLVLYSIKSKIVIFAVIATLLPTISLSLLSFKQNEALISDSVTRELRILADNTNRQLDQ
CEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHEEEEEECCCCHHHHH
WAEENMLAIRALSTSSPVIEGLTTLKQFSDSKSASAVKQTQSVMASYLRSVQIKLDEVLE
HHHCCEEEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTVFDATRKIVASSSTSTPEVREPPEQWTHTSLTSGTIAIPPAWNDRFATATISIIYPVL
HHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHH
SYDSQLIGAIAATLDLGTFESRLAEIKKFSSGEIVLLDQHGKVLLGSAPGVDHQAVLSPH
HCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCCCCHHHCCCC
QWELIQMFDEPISYDGLSYPKAIGLAHTSENIPVTILVEQDHSAIQAAWIKLRNRFLEFV
HHHHHHHHCCCCCCCCCCCCHHHEECCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHH
SILIIIVTAVALYMGHSIVTPLKQLINAVKGIVEGNLDIYLPAKRRDEVGQLTDIFNQMT
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHH
RALRNKHIEIMTANQAMQQQNQLLQKLSITDGLTGLYNRTKLDTILSEQLARFKRHDRPF
HHHHHCCEEEEEHHHHHHHHHHHHHHHHHHCCCHHHHCHHHHHHHHHHHHHHHHHCCCCE
CLLMIDIDYFKTINDKLGHIVGDRILVTVASVLLKSIRTIDYAARYGGDEFVVILTETNS
EEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCC
SAAIKTAERIRAEVNSACLAFKEHPAHITLSIGITQSYYDDITPNDLIARADAALYEAKE
CHHHHHHHHHHHHHHHHHHEEECCCCEEEEEEECCHHHHCCCCCCHHHHHHHHHHHHHHH
SGRDRVHCADA
CCCCCEEECCC
>Mature Secondary Structure
MRLVLYSIKSKIVIFAVIATLLPTISLSLLSFKQNEALISDSVTRELRILADNTNRQLDQ
CEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHEEEEEECCCCHHHHH
WAEENMLAIRALSTSSPVIEGLTTLKQFSDSKSASAVKQTQSVMASYLRSVQIKLDEVLE
HHHCCEEEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTVFDATRKIVASSSTSTPEVREPPEQWTHTSLTSGTIAIPPAWNDRFATATISIIYPVL
HHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHH
SYDSQLIGAIAATLDLGTFESRLAEIKKFSSGEIVLLDQHGKVLLGSAPGVDHQAVLSPH
HCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCCCCHHHCCCC
QWELIQMFDEPISYDGLSYPKAIGLAHTSENIPVTILVEQDHSAIQAAWIKLRNRFLEFV
HHHHHHHHCCCCCCCCCCCCHHHEECCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHH
SILIIIVTAVALYMGHSIVTPLKQLINAVKGIVEGNLDIYLPAKRRDEVGQLTDIFNQMT
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHH
RALRNKHIEIMTANQAMQQQNQLLQKLSITDGLTGLYNRTKLDTILSEQLARFKRHDRPF
HHHHHCCEEEEEHHHHHHHHHHHHHHHHHHCCCHHHHCHHHHHHHHHHHHHHHHHCCCCE
CLLMIDIDYFKTINDKLGHIVGDRILVTVASVLLKSIRTIDYAARYGGDEFVVILTETNS
EEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCC
SAAIKTAERIRAEVNSACLAFKEHPAHITLSIGITQSYYDDITPNDLIARADAALYEAKE
CHHHHHHHHHHHHHHHHHHEEECCCCEEEEEEECCHHHHCCCCCCHHHHHHHHHHHHHHH
SGRDRVHCADA
CCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA