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The map label for this gene is prfB [H]

Identifier: 114331309

GI number: 114331309

Start: 1378930

End: 1379976

Strand: Reverse

Name: prfB [H]

Synonym: Neut_1316

Alternate gene names: 114331309

Gene position: 1379976-1378930 (Counterclockwise)

Preceding gene: 114331310

Following gene: 114331308

Centisome position: 51.86

GC content: 48.42

Gene sequence:

>1047_bases
GTGAAATTTTACGGAGGTATCTTTGACTTTGATGCCAAACAAGTACGGCTGACAGAGCTTGAGCGTCAATCTGAGGATCC
TGCCATCTGGAATGACCCGGCGCGTGCTCAGGAATTAGGGCGCGAAAAAAAATCTCTGGAAAATATCGTGTTCACCCTGC
GATCGATTGATCGCCAATTGCAGGACGCGCAAGAATTGCTGCAGATCGCTCAGGAAGAAAGCGATGATGAAACTTTGCAC
AGTATTTCCGAGGATGTAGCGGGACTTGCCAAAACGGTGGAAGAAATGGAGTTTCACCGAATGTTTTCGGACCCGATGGA
TCCCAATAACTGTTTCATTGATATCCAATCTGGCTCTGGTGGAACGGAAGCACAAGACTGGGCTTCGATGTTGGAAAGAA
TGTATTTGCATTATTGTGAACGACGTGGCCTGGCAGTGGAATTACTGGAAGAATCTCCCGGCGATGTGGCTGGTATCAAG
AGTGCAACAATCAAAGTAAGCGGTGAATATGCCTATGGTTACTTGCGCACGGAAACCGGTGTCCACCGGCTGGTGCGTAA
ATCTCCCTTTGATTCAGGAAATCGCCGGCATACTTCCTTTGCCAGCGTATTTGTCTATCCGGAAGTAGATGACAGTATAG
AAATCGATATCAACCCGGCGGATCTGCGAGTGGATACTTATCGCGCTTCCGGAGCGGGTGGGCAGCACATTAACAAAACT
GATTCAGCTGTCAGAATCACGCATATTCCAACCGGGATTGTTGTTCAATGCCAAAGCGGACGTTCCCAGCATCGGAATCG
TGCCGATGCTATGACAGTGCTGAAATCTCGCCTATATGAAGCGGAATTGAGAAAGCGCAATGAAACCAAACAAGCGATCG
AAGATTCCAAGACGGACATTGCCTGGGGGCACCAGATTCGCTCCTATGTTCTGGATCAATCCCGCATCAAGGATTTGCGC
ACCAATATCGAGGTTGGCAATACTCAGGCCGTACTGGATGGAGACTTAGATGTGTTCATCGAAGCCAGTTTAAAGCAAGG
TGTATAG

Upstream 100 bases:

>100_bases
TCGACTTACAGTATAATCAATGGTTTTGGATGAGATACTGCCATGGAAGCTGAACATATTAATACTTTGACCGAACAACT
GGATGATTTAAAGCACAGGG

Downstream 100 bases:

>100_bases
CGCCCATGACACGGATTGGTGTAAAAATAACTCAAAGTAATATTCCTGGATAAAGTGATGACACCGACTGCTGTGGAATT
AATTGGTACGATATTGTTTG

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MKFYGGIFDFDAKQVRLTELERQSEDPAIWNDPARAQELGREKKSLENIVFTLRSIDRQLQDAQELLQIAQEESDDETLH
SISEDVAGLAKTVEEMEFHRMFSDPMDPNNCFIDIQSGSGGTEAQDWASMLERMYLHYCERRGLAVELLEESPGDVAGIK
SATIKVSGEYAYGYLRTETGVHRLVRKSPFDSGNRRHTSFASVFVYPEVDDSIEIDINPADLRVDTYRASGAGGQHINKT
DSAVRITHIPTGIVVQCQSGRSQHRNRADAMTVLKSRLYEAELRKRNETKQAIEDSKTDIAWGHQIRSYVLDQSRIKDLR
TNIEVGNTQAVLDGDLDVFIEASLKQGV

Sequences:

>Translated_348_residues
MKFYGGIFDFDAKQVRLTELERQSEDPAIWNDPARAQELGREKKSLENIVFTLRSIDRQLQDAQELLQIAQEESDDETLH
SISEDVAGLAKTVEEMEFHRMFSDPMDPNNCFIDIQSGSGGTEAQDWASMLERMYLHYCERRGLAVELLEESPGDVAGIK
SATIKVSGEYAYGYLRTETGVHRLVRKSPFDSGNRRHTSFASVFVYPEVDDSIEIDINPADLRVDTYRASGAGGQHINKT
DSAVRITHIPTGIVVQCQSGRSQHRNRADAMTVLKSRLYEAELRKRNETKQAIEDSKTDIAWGHQIRSYVLDQSRIKDLR
TNIEVGNTQAVLDGDLDVFIEASLKQGV
>Mature_348_residues
MKFYGGIFDFDAKQVRLTELERQSEDPAIWNDPARAQELGREKKSLENIVFTLRSIDRQLQDAQELLQIAQEESDDETLH
SISEDVAGLAKTVEEMEFHRMFSDPMDPNNCFIDIQSGSGGTEAQDWASMLERMYLHYCERRGLAVELLEESPGDVAGIK
SATIKVSGEYAYGYLRTETGVHRLVRKSPFDSGNRRHTSFASVFVYPEVDDSIEIDINPADLRVDTYRASGAGGQHINKT
DSAVRITHIPTGIVVQCQSGRSQHRNRADAMTVLKSRLYEAELRKRNETKQAIEDSKTDIAWGHQIRSYVLDQSRIKDLR
TNIEVGNTQAVLDGDLDVFIEASLKQGV

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=315, Percent_Identity=34.2857142857143, Blast_Score=172, Evalue=4e-43,
Organism=Homo sapiens, GI34577120, Length=318, Percent_Identity=33.0188679245283, Blast_Score=164, Evalue=9e-41,
Organism=Homo sapiens, GI166795305, Length=214, Percent_Identity=34.1121495327103, Blast_Score=125, Evalue=8e-29,
Organism=Escherichia coli, GI2367172, Length=343, Percent_Identity=63.5568513119534, Blast_Score=468, Evalue=1e-133,
Organism=Escherichia coli, GI1787462, Length=325, Percent_Identity=38.7692307692308, Blast_Score=211, Evalue=4e-56,
Organism=Caenorhabditis elegans, GI17542784, Length=221, Percent_Identity=33.9366515837104, Blast_Score=107, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6321295, Length=251, Percent_Identity=37.0517928286853, Blast_Score=146, Evalue=5e-36,
Organism=Drosophila melanogaster, GI19921226, Length=236, Percent_Identity=40.6779661016949, Blast_Score=160, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 39261; Mature: 39261

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFYGGIFDFDAKQVRLTELERQSEDPAIWNDPARAQELGREKKSLENIVFTLRSIDRQL
CCCCCCEECCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QDAQELLQIAQEESDDETLHSISEDVAGLAKTVEEMEFHRMFSDPMDPNNCFIDIQSGSG
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCC
GTEAQDWASMLERMYLHYCERRGLAVELLEESPGDVAGIKSATIKVSGEYAYGYLRTETG
CCCHHHHHHHHHHHHHHHHHHCCCEEEEHHCCCCCEECCCEEEEEEECCEEEEEEECHHH
VHRLVRKSPFDSGNRRHTSFASVFVYPEVDDSIEIDINPADLRVDTYRASGAGGQHINKT
HHHHHHCCCCCCCCCCCCEEEEEEEEECCCCCEEEEECHHHEEEEEEECCCCCCCCCCCC
DSAVRITHIPTGIVVQCQSGRSQHRNRADAMTVLKSRLYEAELRKRNETKQAIEDSKTDI
CCEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
AWGHQIRSYVLDQSRIKDLRTNIEVGNTQAVLDGDLDVFIEASLKQGV
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEEECHHCCC
>Mature Secondary Structure
MKFYGGIFDFDAKQVRLTELERQSEDPAIWNDPARAQELGREKKSLENIVFTLRSIDRQL
CCCCCCEECCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QDAQELLQIAQEESDDETLHSISEDVAGLAKTVEEMEFHRMFSDPMDPNNCFIDIQSGSG
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCC
GTEAQDWASMLERMYLHYCERRGLAVELLEESPGDVAGIKSATIKVSGEYAYGYLRTETG
CCCHHHHHHHHHHHHHHHHHHCCCEEEEHHCCCCCEECCCEEEEEEECCEEEEEEECHHH
VHRLVRKSPFDSGNRRHTSFASVFVYPEVDDSIEIDINPADLRVDTYRASGAGGQHINKT
HHHHHHCCCCCCCCCCCCEEEEEEEEECCCCCEEEEECHHHEEEEEEECCCCCCCCCCCC
DSAVRITHIPTGIVVQCQSGRSQHRNRADAMTVLKSRLYEAELRKRNETKQAIEDSKTDI
CCEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
AWGHQIRSYVLDQSRIKDLRTNIEVGNTQAVLDGDLDVFIEASLKQGV
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEEECHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA