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The map label for this gene is ilvI [H]

Identifier: 114331257

GI number: 114331257

Start: 1317486

End: 1319189

Strand: Reverse

Name: ilvI [H]

Synonym: Neut_1262

Alternate gene names: 114331257

Gene position: 1319189-1317486 (Counterclockwise)

Preceding gene: 114331258

Following gene: 114331256

Centisome position: 49.57

GC content: 45.77

Gene sequence:

>1704_bases
ATGAGTACTGAATTGACAGGTGCTGAGATCACAATACGCTGTCTGCAGGAGGAAGGGGTAAGTCATATTTTTGGTTATCC
TGGTGGTGCCGTGTTGTTCCTGTACGATGAATTGTTCAAACAAGATAAAATCAAGCATATTCTGGTTCGCCATGAGCAGG
CTGCGCTCCACGCAGCGGATGGCTATGCACGTTCCAGCAATAAAGTAGGCGTGGCTTTGGTTACATCTGGCCCGGGTGTT
ACGAATGCTGTAACGGGCATTGCTACCGCCTTCATGGATTCGATTCCAATGGTGATTATCAGCGGACAGGTGCCAACGGC
TGCCATTGGTCAGGATGCGTTCCAGGAAGTGGACACAGTAGGGATTACGCGTCCTTGCGTTAAACATAACTTTCTGGTGA
AAGACGTGGCAGAGCTGGCCACAACGATCAAAAAAGCTTTTTATATTGCATCAACAGGACGTCCTGGGCCCGTTTTAGTG
GATATACCCAAGGATGTTACGCAGCAAAAGGCCGAATTTAATTATCCTGCCAGTATTTCACTGCGCTCCTACACTCCAGT
AGTTACCGGAGATGTTCAGCAGATTAAAAGAGCCATCCAGATGATTTTGGAAGCAAAACGCCCCATGGTCTATTTTGGGG
GTGGTGTGATTTTGGATAATGCTGCAGCAGAATTAACGGAATTCGTACGGATGCTGAATTTTCCGTGTACCGGTACACTC
ATGGGGCTGGGTGGTTATCCATCAACTGATCAGCAGTTTGTCGGAATGCTGGGTATGCATGGTACCTACGAGGCGAACAT
GGCTATGCAATATTGCGATGTGCTGATCGCGGTAGGCGCCCGCTTTGATGATCGGGTTATCGGAAATCCCAAACATTTCT
GTAATGAAGAAAGGAAAATTATCCATATTGATATCGACCCGTCATCAATTTCCAAACGTGTCAAAGTAGATGTTCCTATT
GTAGGCGGCGTATCGGCAGTATTAAAAGAGCTGAACATGTTGCTCAAGGCTGGCAGGGAAACAACGGATATTAATGCGCT
ACTTAAATGGTGGGAACAGATTGAGCTGTGGCGTGCACGTGACTGTCTGAAATATGATAGGACGGCTAATATTATTAAAC
CTCAAATGGTGGTCGAAAAGTTATATGAAATAACCAGTGGGGATGCCTTTATTACATCTGATGTTGGGCAACATCAAATG
TGGGCAGCACAGTTTTATAAATTTGATAAGCCACGCCGCTGGATCAATTCAGGAGGTCTGGGTACGATGGGATTCGGCTT
GCCTGCTGCAATGGGTGTGCAAATGGCCAACCCGGGAAGCAAGGTGGCCTGTATTACCGGGGAAGCAAGTATTCAGATGT
GTATACAAGAATTATCCACTTGTAAACAATATCATCTGCCCATCAAAATCATCAACCTTAACAATCGCTACATGGGAATG
GTGCGGCAGTGGCAAGAGTTTTTCCACGGCAACCGCTATGCTGAATCCTATGTGGATGCATTACCTGATTTTGTTAAGCT
TGCTGAAAGTTATGGGCATGTCGGCATGCGAATTGATAAACCAGAAGATATTGAAGGTACGCTCAAAGAAGCCTTCAAGC
TGGATGAGCAGCTTGTATTCATAGACTTTATTACAGACCAGACCGAGAACGTTTTTCCTATGGTGCCTGGTGGAAAAGGT
CTATCCGAAATGATTTTGGTATAA

Upstream 100 bases:

>100_bases
CTTTATTGCTATGTAAGCAGCAAACGATATAAAGAAAGAATTGGCATTGGTTTTAATTACAAGATGAAAAAAATTATTTT
AAATCTGGGAATTTCCTGGT

Downstream 100 bases:

>100_bases
CAGTATGCGACATATTATTTCTTTGTTAATGGAAAATGAGGCTGGCGCACTATCGCGAGTAGCCGGTTTGTTTTCTGCTC
GTGGTTACAATATTGAATCC

Product: acetolactate synthase, large subunit, biosynthetic type

Products: NA

Alternate protein names: AHAS-III; ALS-III; Acetohydroxy-acid synthase III large subunit [H]

Number of amino acids: Translated: 567; Mature: 566

Protein sequence:

>567_residues
MSTELTGAEITIRCLQEEGVSHIFGYPGGAVLFLYDELFKQDKIKHILVRHEQAALHAADGYARSSNKVGVALVTSGPGV
TNAVTGIATAFMDSIPMVIISGQVPTAAIGQDAFQEVDTVGITRPCVKHNFLVKDVAELATTIKKAFYIASTGRPGPVLV
DIPKDVTQQKAEFNYPASISLRSYTPVVTGDVQQIKRAIQMILEAKRPMVYFGGGVILDNAAAELTEFVRMLNFPCTGTL
MGLGGYPSTDQQFVGMLGMHGTYEANMAMQYCDVLIAVGARFDDRVIGNPKHFCNEERKIIHIDIDPSSISKRVKVDVPI
VGGVSAVLKELNMLLKAGRETTDINALLKWWEQIELWRARDCLKYDRTANIIKPQMVVEKLYEITSGDAFITSDVGQHQM
WAAQFYKFDKPRRWINSGGLGTMGFGLPAAMGVQMANPGSKVACITGEASIQMCIQELSTCKQYHLPIKIINLNNRYMGM
VRQWQEFFHGNRYAESYVDALPDFVKLAESYGHVGMRIDKPEDIEGTLKEAFKLDEQLVFIDFITDQTENVFPMVPGGKG
LSEMILV

Sequences:

>Translated_567_residues
MSTELTGAEITIRCLQEEGVSHIFGYPGGAVLFLYDELFKQDKIKHILVRHEQAALHAADGYARSSNKVGVALVTSGPGV
TNAVTGIATAFMDSIPMVIISGQVPTAAIGQDAFQEVDTVGITRPCVKHNFLVKDVAELATTIKKAFYIASTGRPGPVLV
DIPKDVTQQKAEFNYPASISLRSYTPVVTGDVQQIKRAIQMILEAKRPMVYFGGGVILDNAAAELTEFVRMLNFPCTGTL
MGLGGYPSTDQQFVGMLGMHGTYEANMAMQYCDVLIAVGARFDDRVIGNPKHFCNEERKIIHIDIDPSSISKRVKVDVPI
VGGVSAVLKELNMLLKAGRETTDINALLKWWEQIELWRARDCLKYDRTANIIKPQMVVEKLYEITSGDAFITSDVGQHQM
WAAQFYKFDKPRRWINSGGLGTMGFGLPAAMGVQMANPGSKVACITGEASIQMCIQELSTCKQYHLPIKIINLNNRYMGM
VRQWQEFFHGNRYAESYVDALPDFVKLAESYGHVGMRIDKPEDIEGTLKEAFKLDEQLVFIDFITDQTENVFPMVPGGKG
LSEMILV
>Mature_566_residues
STELTGAEITIRCLQEEGVSHIFGYPGGAVLFLYDELFKQDKIKHILVRHEQAALHAADGYARSSNKVGVALVTSGPGVT
NAVTGIATAFMDSIPMVIISGQVPTAAIGQDAFQEVDTVGITRPCVKHNFLVKDVAELATTIKKAFYIASTGRPGPVLVD
IPKDVTQQKAEFNYPASISLRSYTPVVTGDVQQIKRAIQMILEAKRPMVYFGGGVILDNAAAELTEFVRMLNFPCTGTLM
GLGGYPSTDQQFVGMLGMHGTYEANMAMQYCDVLIAVGARFDDRVIGNPKHFCNEERKIIHIDIDPSSISKRVKVDVPIV
GGVSAVLKELNMLLKAGRETTDINALLKWWEQIELWRARDCLKYDRTANIIKPQMVVEKLYEITSGDAFITSDVGQHQMW
AAQFYKFDKPRRWINSGGLGTMGFGLPAAMGVQMANPGSKVACITGEASIQMCIQELSTCKQYHLPIKIINLNNRYMGMV
RQWQEFFHGNRYAESYVDALPDFVKLAESYGHVGMRIDKPEDIEGTLKEAFKLDEQLVFIDFITDQTENVFPMVPGGKGL
SEMILV

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=545, Percent_Identity=25.6880733944954, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI21361361, Length=565, Percent_Identity=25.8407079646018, Blast_Score=147, Evalue=2e-35,
Organism=Escherichia coli, GI87081685, Length=566, Percent_Identity=56.1837455830389, Blast_Score=680, Evalue=0.0,
Organism=Escherichia coli, GI1790104, Length=564, Percent_Identity=41.3120567375887, Blast_Score=417, Evalue=1e-118,
Organism=Escherichia coli, GI1786717, Length=566, Percent_Identity=33.3922261484099, Blast_Score=314, Evalue=1e-86,
Organism=Escherichia coli, GI1787096, Length=553, Percent_Identity=27.6672694394213, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI1788716, Length=479, Percent_Identity=24.6346555323591, Blast_Score=149, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI17531299, Length=562, Percent_Identity=26.8683274021352, Blast_Score=167, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17531301, Length=562, Percent_Identity=26.8683274021352, Blast_Score=166, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17542570, Length=595, Percent_Identity=25.0420168067227, Blast_Score=146, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6323755, Length=583, Percent_Identity=44.082332761578, Blast_Score=451, Evalue=1e-127,
Organism=Saccharomyces cerevisiae, GI6320816, Length=558, Percent_Identity=24.5519713261649, Blast_Score=118, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6320123, Length=552, Percent_Identity=22.1014492753623, Blast_Score=85, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6321524, Length=494, Percent_Identity=22.8744939271255, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323163, Length=494, Percent_Identity=22.0647773279352, Blast_Score=71, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6323073, Length=500, Percent_Identity=21.8, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI19922626, Length=486, Percent_Identity=27.1604938271605, Blast_Score=172, Evalue=7e-43,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 62661; Mature: 62530

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTELTGAEITIRCLQEEGVSHIFGYPGGAVLFLYDELFKQDKIKHILVRHEQAALHAAD
CCCCCCCCCEEEEEEHHCCCCEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GYARSSNKVGVALVTSGPGVTNAVTGIATAFMDSIPMVIISGQVPTAAIGQDAFQEVDTV
CCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHCHHHHHHHHHC
GITRPCVKHNFLVKDVAELATTIKKAFYIASTGRPGPVLVDIPKDVTQQKAEFNYPASIS
CCCCHHHHCCHHHHHHHHHHHHHHHHEEEEECCCCCCEEEECCHHHHHHHHCCCCCCEEE
LRSYTPVVTGDVQQIKRAIQMILEAKRPMVYFGGGVILDNAAAELTEFVRMLNFPCTGTL
ECCCCCEEECCHHHHHHHHHHHHHCCCCEEEECCCEEECCHHHHHHHHHHHHCCCCCCEE
MGLGGYPSTDQQFVGMLGMHGTYEANMAMQYCDVLIAVGARFDDRVIGNPKHFCNEERKI
EECCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCEE
IHIDIDPSSISKRVKVDVPIVGGVSAVLKELNMLLKAGRETTDINALLKWWEQIELWRAR
EEEEECHHHHCCEEEEECCEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
DCLKYDRTANIIKPQMVVEKLYEITSGDAFITSDVGQHQMWAAQFYKFDKPRRWINSGGL
HHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCCCCHHHHCCCCC
GTMGFGLPAAMGVQMANPGSKVACITGEASIQMCIQELSTCKQYHLPIKIINLNNRYMGM
CCCCCCCHHHHCEEECCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHH
VRQWQEFFHGNRYAESYVDALPDFVKLAESYGHVGMRIDKPEDIEGTLKEAFKLDEQLVF
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHCCCCEEEE
IDFITDQTENVFPMVPGGKGLSEMILV
EEEEECCCCCEEEECCCCCCHHHHHCC
>Mature Secondary Structure 
STELTGAEITIRCLQEEGVSHIFGYPGGAVLFLYDELFKQDKIKHILVRHEQAALHAAD
CCCCCCCCEEEEEEHHCCCCEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GYARSSNKVGVALVTSGPGVTNAVTGIATAFMDSIPMVIISGQVPTAAIGQDAFQEVDTV
CCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHCHHHHHHHHHC
GITRPCVKHNFLVKDVAELATTIKKAFYIASTGRPGPVLVDIPKDVTQQKAEFNYPASIS
CCCCHHHHCCHHHHHHHHHHHHHHHHEEEEECCCCCCEEEECCHHHHHHHHCCCCCCEEE
LRSYTPVVTGDVQQIKRAIQMILEAKRPMVYFGGGVILDNAAAELTEFVRMLNFPCTGTL
ECCCCCEEECCHHHHHHHHHHHHHCCCCEEEECCCEEECCHHHHHHHHHHHHCCCCCCEE
MGLGGYPSTDQQFVGMLGMHGTYEANMAMQYCDVLIAVGARFDDRVIGNPKHFCNEERKI
EECCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCEE
IHIDIDPSSISKRVKVDVPIVGGVSAVLKELNMLLKAGRETTDINALLKWWEQIELWRAR
EEEEECHHHHCCEEEEECCEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
DCLKYDRTANIIKPQMVVEKLYEITSGDAFITSDVGQHQMWAAQFYKFDKPRRWINSGGL
HHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCCCCHHHHCCCCC
GTMGFGLPAAMGVQMANPGSKVACITGEASIQMCIQELSTCKQYHLPIKIINLNNRYMGM
CCCCCCCHHHHCEEECCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHH
VRQWQEFFHGNRYAESYVDALPDFVKLAESYGHVGMRIDKPEDIEGTLKEAFKLDEQLVF
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHCCCCEEEE
IDFITDQTENVFPMVPGGKGLSEMILV
EEEEECCCCCEEEECCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6308579; 1630901; 9278503; 3891724; 9298646 [H]