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The map label for this gene is proS

Identifier: 114331243

GI number: 114331243

Start: 1302726

End: 1304432

Strand: Reverse

Name: proS

Synonym: Neut_1247

Alternate gene names: 114331243

Gene position: 1304432-1302726 (Counterclockwise)

Preceding gene: 114331248

Following gene: 114331242

Centisome position: 49.02

GC content: 46.4

Gene sequence:

>1707_bases
ATGCGTGTTTCCCAATTCTTCCTTTCGACGCTCAAAGAAGCTCCTGCTGAAGCCGAGCTGGCCAGTCACCGGTTGATGTT
GCGGGCCGGGCTGATTAAGCGTCTGGGCAGCGGATTGTATACCTGGATGCCGCTTGGTCTGAGAATTCTACACAAGATTG
AACATATCATCCGTGAGGAAATGAACAGCAGTGGTGCGCTAGAGCTTCTGATGCCCGCTGTTCATCCAGCTGAACTTTGG
CAGGAATCAGGTCGCTGGGATGTATTTGGTCCGCAAATGCTGAAAATTCAAGATAGGCATCAGCATGATTTCTGCTTTGG
GCCGACACATGAAGAAGTTATTGTTGATATTGCTCGTCGTGAAATTAAAAGCTATCGGCAACTGCCTATTAATTTCTATC
AGATTCAGACCAAGTTTCGTGATGAAATCCGCCCACGTTTTGGTGTGATGCGCGCCAGGGAATTTATCATGAAGGATGCC
TACTCCTTTCATGCTGATCTGGGCAGTCTGGAACAAACCTACCAGCTTATGCATGAAACATACAGCAGAATTTTTACCCG
GATCGGATTGAAATTTCGCGCAGTGGCTGCTGATACTGGGGCGATAGGGGGTAGCGGATCACATGAATTTCACGTGCTGG
CTGATTCAGGTGAGGATGCTATCGCATTTTGCCCTGATTCCGATTATGCCGCCAATATTGAACTTGCTGAAGCAATATCA
CATGGAATTCTGCGTAAGGATCCGGCGGGTGTGATGAAAAAAATTGCTACCCCTGATCGTAAATCCTGTTCGGATGTTGC
TGAATTTCTGAGCGTTCCAATAGAACAGACTCTTAAAACACTGGCAGTGATAGCCGATGGCCGATTTTATCTACTGCTGC
TGCGAGGCGATCATCAGCTCAATGAAACAAAAACCAGAAAAATCCCGTTTCTGAGTAACTTCGAGTTTGCTGATGAAAGC
AGAATTATTGCTAAAACTGGTTGCCTTCCAGGATATCTTGGTCCGATCGGTGTGAAAACTGAAATTATCGCTGATCGGGC
AGTTCTGGAAATGAGCAATTTCGTCTGCGGGGCAAATGAAGAAGGCTATCACCTGACTCAAGTTAATTTTGAACGTGATC
TGCCATTGCCAGTCCAGGTATTCGATATCCGCAATGTGGTTACAGGTGATCCTTCGCCTGATGGAAAAGGAATGCTCGAA
ATTTGCCGTGGTATTGAGGTTGGTCATATTTTCCAGTTGCGCACCAAATATTCTGAAAAAATGAAAGCAACCTATCTGGA
TGAATCTGGCCAGACTCAAATTCTGGAAATGGGTTGTTATGGTATCGGTGTTTCACGCATCGTTGCTGCGGCTATTGAGC
AAAACTGTGATGAGCGCGGCATCATATTTCCAGTAGCAATTGCTCCGTTTCAGTTGTCGATTATCCCGATTGGTTATCAT
AAAAACCTTCAGATCCAGGCTGAAGTGGAAAAACTGTATCGAGCATGTCGTGCAGCAGGCATCGAAGTATTGCTGGATGA
CCGGGAGGAGCGACCAGGTGTTATGTTTGCAGATCAGGAGCTGATTGGGATTCCGCACCGTATTGTTATTGGTGAGCGAA
ATTTGCGGGATGGGATGGTGGAATACCAGGGGAGGCTAGATAAAACATCCCGTTTACTTTCTCTACAGGAGGTTATCCCT
GTTATCAGGGAAATATGTGGCGGCTGA

Upstream 100 bases:

>100_bases
ACTTGCAATGCTGTAGTCAATTCCCGCTAAAGCGTACCGGATCAAAACAGTTTCAAGGTAAAATTATCTTATTACCTACT
ATGATAAATGGAACCTCATC

Downstream 100 bases:

>100_bases
TAAATTTTCTATCATTTTTCATTGCAAGTACGGCTATGGCAGGGTCTCAGGCTTATGAATCCTTGTCGGTCAGCACCCGG
ACAGTATTACAGCGTACCGT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 568; Mature: 568

Protein sequence:

>568_residues
MRVSQFFLSTLKEAPAEAELASHRLMLRAGLIKRLGSGLYTWMPLGLRILHKIEHIIREEMNSSGALELLMPAVHPAELW
QESGRWDVFGPQMLKIQDRHQHDFCFGPTHEEVIVDIARREIKSYRQLPINFYQIQTKFRDEIRPRFGVMRAREFIMKDA
YSFHADLGSLEQTYQLMHETYSRIFTRIGLKFRAVAADTGAIGGSGSHEFHVLADSGEDAIAFCPDSDYAANIELAEAIS
HGILRKDPAGVMKKIATPDRKSCSDVAEFLSVPIEQTLKTLAVIADGRFYLLLLRGDHQLNETKTRKIPFLSNFEFADES
RIIAKTGCLPGYLGPIGVKTEIIADRAVLEMSNFVCGANEEGYHLTQVNFERDLPLPVQVFDIRNVVTGDPSPDGKGMLE
ICRGIEVGHIFQLRTKYSEKMKATYLDESGQTQILEMGCYGIGVSRIVAAAIEQNCDERGIIFPVAIAPFQLSIIPIGYH
KNLQIQAEVEKLYRACRAAGIEVLLDDREERPGVMFADQELIGIPHRIVIGERNLRDGMVEYQGRLDKTSRLLSLQEVIP
VIREICGG

Sequences:

>Translated_568_residues
MRVSQFFLSTLKEAPAEAELASHRLMLRAGLIKRLGSGLYTWMPLGLRILHKIEHIIREEMNSSGALELLMPAVHPAELW
QESGRWDVFGPQMLKIQDRHQHDFCFGPTHEEVIVDIARREIKSYRQLPINFYQIQTKFRDEIRPRFGVMRAREFIMKDA
YSFHADLGSLEQTYQLMHETYSRIFTRIGLKFRAVAADTGAIGGSGSHEFHVLADSGEDAIAFCPDSDYAANIELAEAIS
HGILRKDPAGVMKKIATPDRKSCSDVAEFLSVPIEQTLKTLAVIADGRFYLLLLRGDHQLNETKTRKIPFLSNFEFADES
RIIAKTGCLPGYLGPIGVKTEIIADRAVLEMSNFVCGANEEGYHLTQVNFERDLPLPVQVFDIRNVVTGDPSPDGKGMLE
ICRGIEVGHIFQLRTKYSEKMKATYLDESGQTQILEMGCYGIGVSRIVAAAIEQNCDERGIIFPVAIAPFQLSIIPIGYH
KNLQIQAEVEKLYRACRAAGIEVLLDDREERPGVMFADQELIGIPHRIVIGERNLRDGMVEYQGRLDKTSRLLSLQEVIP
VIREICGG
>Mature_568_residues
MRVSQFFLSTLKEAPAEAELASHRLMLRAGLIKRLGSGLYTWMPLGLRILHKIEHIIREEMNSSGALELLMPAVHPAELW
QESGRWDVFGPQMLKIQDRHQHDFCFGPTHEEVIVDIARREIKSYRQLPINFYQIQTKFRDEIRPRFGVMRAREFIMKDA
YSFHADLGSLEQTYQLMHETYSRIFTRIGLKFRAVAADTGAIGGSGSHEFHVLADSGEDAIAFCPDSDYAANIELAEAIS
HGILRKDPAGVMKKIATPDRKSCSDVAEFLSVPIEQTLKTLAVIADGRFYLLLLRGDHQLNETKTRKIPFLSNFEFADES
RIIAKTGCLPGYLGPIGVKTEIIADRAVLEMSNFVCGANEEGYHLTQVNFERDLPLPVQVFDIRNVVTGDPSPDGKGMLE
ICRGIEVGHIFQLRTKYSEKMKATYLDESGQTQILEMGCYGIGVSRIVAAAIEQNCDERGIIFPVAIAPFQLSIIPIGYH
KNLQIQAEVEKLYRACRAAGIEVLLDDREERPGVMFADQELIGIPHRIVIGERNLRDGMVEYQGRLDKTSRLLSLQEVIP
VIREICGG

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=226, Percent_Identity=44.2477876106195, Blast_Score=219, Evalue=5e-57,
Organism=Escherichia coli, GI1786392, Length=573, Percent_Identity=57.7661431064572, Blast_Score=684, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115532348, Length=210, Percent_Identity=40.4761904761905, Blast_Score=172, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI193203271, Length=122, Percent_Identity=28.6885245901639, Blast_Score=79, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6320931, Length=539, Percent_Identity=29.6846011131725, Blast_Score=231, Evalue=2e-61,
Organism=Drosophila melanogaster, GI24656200, Length=218, Percent_Identity=37.6146788990826, Blast_Score=169, Evalue=3e-42,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_NITEC (Q0AGN2)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_747465.1
- ProteinModelPortal:   Q0AGN2
- SMR:   Q0AGN2
- STRING:   Q0AGN2
- GeneID:   4272191
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_1247
- NMPDR:   fig|335283.3.peg.1959
- eggNOG:   COG0442
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- PhylomeDB:   Q0AGN2
- ProtClustDB:   PRK09194
- BioCyc:   NEUT335283:NEUT_1247-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.90.960.10
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 63918; Mature: 63918

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVSQFFLSTLKEAPAEAELASHRLMLRAGLIKRLGSGLYTWMPLGLRILHKIEHIIREE
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
MNSSGALELLMPAVHPAELWQESGRWDVFGPQMLKIQDRHQHDFCFGPTHEEVIVDIARR
CCCCCCEEHHHCCCCHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHH
EIKSYRQLPINFYQIQTKFRDEIRPRFGVMRAREFIMKDAYSFHADLGSLEQTYQLMHET
HHHHHHCCCCEEEEHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YSRIFTRIGLKFRAVAADTGAIGGSGSHEFHVLADSGEDAIAFCPDSDYAANIELAEAIS
HHHHHHHHCCEEEEEECCCCCCCCCCCCEEEEEECCCCCEEEECCCCCCCCCCHHHHHHH
HGILRKDPAGVMKKIATPDRKSCSDVAEFLSVPIEQTLKTLAVIADGRFYLLLLRGDHQL
HCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHEECCCEEEEEEECCCCC
NETKTRKIPFLSNFEFADESRIIAKTGCLPGYLGPIGVKTEIIADRAVLEMSNFVCGANE
CCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCEECCCC
EGYHLTQVNFERDLPLPVQVFDIRNVVTGDPSPDGKGMLEICRGIEVGHIFQLRTKYSEK
CCEEEEEEECCCCCCCCEEEEEHHHHHCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHH
MKATYLDESGQTQILEMGCYGIGVSRIVAAAIEQNCDERGIIFPVAIAPFQLSIIPIGYH
HHHHCCCCCCCEEEEECCCHHCCHHHHHHHHHHCCCCCCCCEEEEEECCEEEEEEEECCC
KNLQIQAEVEKLYRACRAAGIEVLLDDREERPGVMFADQELIGIPHRIVIGERNLRDGMV
CCEEEEHHHHHHHHHHHHCCCEEEECCCCCCCCEEEECCHHHCCCCEEEECCCCCCCCHH
EYQGRLDKTSRLLSLQEVIPVIREICGG
HHHCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRVSQFFLSTLKEAPAEAELASHRLMLRAGLIKRLGSGLYTWMPLGLRILHKIEHIIREE
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
MNSSGALELLMPAVHPAELWQESGRWDVFGPQMLKIQDRHQHDFCFGPTHEEVIVDIARR
CCCCCCEEHHHCCCCHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHH
EIKSYRQLPINFYQIQTKFRDEIRPRFGVMRAREFIMKDAYSFHADLGSLEQTYQLMHET
HHHHHHCCCCEEEEHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YSRIFTRIGLKFRAVAADTGAIGGSGSHEFHVLADSGEDAIAFCPDSDYAANIELAEAIS
HHHHHHHHCCEEEEEECCCCCCCCCCCCEEEEEECCCCCEEEECCCCCCCCCCHHHHHHH
HGILRKDPAGVMKKIATPDRKSCSDVAEFLSVPIEQTLKTLAVIADGRFYLLLLRGDHQL
HCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHEECCCEEEEEEECCCCC
NETKTRKIPFLSNFEFADESRIIAKTGCLPGYLGPIGVKTEIIADRAVLEMSNFVCGANE
CCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCEECCCC
EGYHLTQVNFERDLPLPVQVFDIRNVVTGDPSPDGKGMLEICRGIEVGHIFQLRTKYSEK
CCEEEEEEECCCCCCCCEEEEEHHHHHCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHH
MKATYLDESGQTQILEMGCYGIGVSRIVAAAIEQNCDERGIIFPVAIAPFQLSIIPIGYH
HHHHCCCCCCCEEEEECCCHHCCHHHHHHHHHHCCCCCCCCEEEEEECCEEEEEEEECCC
KNLQIQAEVEKLYRACRAAGIEVLLDDREERPGVMFADQELIGIPHRIVIGERNLRDGMV
CCEEEEHHHHHHHHHHHHCCCEEEECCCCCCCCEEEECCHHHCCCCEEEECCCCCCCCHH
EYQGRLDKTSRLLSLQEVIPVIREICGG
HHHCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA