Click here to switch to the map view.

The map label for this gene is leuA2 [H]

Identifier: 113477221

GI number: 113477221

Start: 5772259

End: 5773647

Strand: Reverse

Name: leuA2 [H]

Synonym: Tery_3757

Alternate gene names: 113477221

Gene position: 5773647-5772259 (Counterclockwise)

Preceding gene: 113477222

Following gene: 113477218

Centisome position: 74.5

GC content: 38.88

Gene sequence:

>1389_bases
ATGGTTCAAATTGTAGATAGCACCCTAAGAGAAGGAAGGCAGACTGCCCCTGGGATAAATTTTAGTTGGGAACAATCCCT
GAAGATTGCTAAACTACTTCATAGTCTGGGCATTGATATAATTGAAGTCTGCCACCCTGTTGTTTCTATTGAGCAAAAAC
AAATTGTCCGAGAAATTGTAGCTTCAAATCTCTGCCCAGTTATTGCTCATGCACGTGCTAATTTAGCTGATATTGAGGCA
GTCAAAGAAACATCGGCTCAATGGGTTGGTATATTCTTGGGAATTAATGAAGAAACTAGGCTGACTCGTGCGAGATGGAC
TATTAGCAAAATTTTTAACTGCATCAAAGAGTCAGTAATGTATGCCAAAACCCTTGGTTTGAAGGTAAGATATACTGCTG
AAGATGCTTCTCGCACTGACTATTGTTTGCTAAAAAGCGTTTATAGTGTAGCTATAGAAGCTGGTGCAGATTGCATTTGC
TTTTCTGATACAGTAGGAATCATGGAGCCTAATGAGGTTACTGAGACGATTGAAAAGTTGAAGAGTGATTTTTCAACAGT
ACCTTTAGAAGTCCATTTTCATAATGATCGGGGTTTGGCAATGGCTAATGCTCTTGCTGCTTCTGATGCTGGCGCAGAAA
GGATTTCTTCTTCTGTTAACGGGTTAGGGGAAAGATGTGGCATAACTGATACATTAGGTCTGTTAGTTAATCTTTATTAT
AAGGGACTGCGTTCCCTACCGTCAGGAGAGATAATTCAACGAACAAGTAAGTATGTAGCAGCTTTTTCGCGTTACCCTGT
TAATTTTAACCAACCAATCTCAGGGGGGAATGCTTTTATTCACACTGCTCATCTACATCAAAAAGCAGTCAAACGTGATC
CTTCTAGCTATAGCTGGATTTTGCCAGAATATGTGGGGAAAACTATGGAGTTATCTGGTAAAAGCATACCCTCTGTTCAT
TCTTTATTTCTTACACCAATAGTAAAGTGTGCTAGTGAGCTTGGATATCATAGATATGGAGATAGTGTAGGACATTTGAT
GATTGATGAGTGCATGGTTCCAAACTGTCGGCAATCTTGTATTGTACGTGAAATAAGAGTGGTGGAAGAAAAACCTCTTG
CTTATGTGGATGTTCAGACTCTTTATGTAGATAGCTTGTTCTTGTTTTTAGGTCACGAACTTGGTATGAAAGGATTGCAG
GTTGAAGTATGTCTAGGACAAGTTGTTCAAAAAATTGATAGTCCAGCAAGTGTGTTTATTCCAGCAGGTGTAGCTCACAC
TTATCGAATTCTTAATGGTAGTGGCTTTTGCATAAACCACGTTCTTTCTGGTTCTTATAATGATAGTCTTTTAGAACCTT
TAGATATATCTAGCAATAGTTATAGCTGA

Upstream 100 bases:

>100_bases
ACTTTTACCTTGGCCTAATTTACCTGAAAAACTTTGGCCTAGACGTCAATCTATAGACTGTTAATTGCAAATAACTATAT
TTAACAAAAAAAAATCAACA

Downstream 100 bases:

>100_bases
GCTCAGGACTCAGAATTCAGAACTCAGAAGTTAAATTCTTGCTATGACTAAAATTGAGAGGTGTGAGAATGTTCTAAGAT
TAATGGGGGCTGCTACATGT

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase 2; Alpha-isopropylmalate synthase 2 [H]

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MVQIVDSTLREGRQTAPGINFSWEQSLKIAKLLHSLGIDIIEVCHPVVSIEQKQIVREIVASNLCPVIAHARANLADIEA
VKETSAQWVGIFLGINEETRLTRARWTISKIFNCIKESVMYAKTLGLKVRYTAEDASRTDYCLLKSVYSVAIEAGADCIC
FSDTVGIMEPNEVTETIEKLKSDFSTVPLEVHFHNDRGLAMANALAASDAGAERISSSVNGLGERCGITDTLGLLVNLYY
KGLRSLPSGEIIQRTSKYVAAFSRYPVNFNQPISGGNAFIHTAHLHQKAVKRDPSSYSWILPEYVGKTMELSGKSIPSVH
SLFLTPIVKCASELGYHRYGDSVGHLMIDECMVPNCRQSCIVREIRVVEEKPLAYVDVQTLYVDSLFLFLGHELGMKGLQ
VEVCLGQVVQKIDSPASVFIPAGVAHTYRILNGSGFCINHVLSGSYNDSLLEPLDISSNSYS

Sequences:

>Translated_462_residues
MVQIVDSTLREGRQTAPGINFSWEQSLKIAKLLHSLGIDIIEVCHPVVSIEQKQIVREIVASNLCPVIAHARANLADIEA
VKETSAQWVGIFLGINEETRLTRARWTISKIFNCIKESVMYAKTLGLKVRYTAEDASRTDYCLLKSVYSVAIEAGADCIC
FSDTVGIMEPNEVTETIEKLKSDFSTVPLEVHFHNDRGLAMANALAASDAGAERISSSVNGLGERCGITDTLGLLVNLYY
KGLRSLPSGEIIQRTSKYVAAFSRYPVNFNQPISGGNAFIHTAHLHQKAVKRDPSSYSWILPEYVGKTMELSGKSIPSVH
SLFLTPIVKCASELGYHRYGDSVGHLMIDECMVPNCRQSCIVREIRVVEEKPLAYVDVQTLYVDSLFLFLGHELGMKGLQ
VEVCLGQVVQKIDSPASVFIPAGVAHTYRILNGSGFCINHVLSGSYNDSLLEPLDISSNSYS
>Mature_462_residues
MVQIVDSTLREGRQTAPGINFSWEQSLKIAKLLHSLGIDIIEVCHPVVSIEQKQIVREIVASNLCPVIAHARANLADIEA
VKETSAQWVGIFLGINEETRLTRARWTISKIFNCIKESVMYAKTLGLKVRYTAEDASRTDYCLLKSVYSVAIEAGADCIC
FSDTVGIMEPNEVTETIEKLKSDFSTVPLEVHFHNDRGLAMANALAASDAGAERISSSVNGLGERCGITDTLGLLVNLYY
KGLRSLPSGEIIQRTSKYVAAFSRYPVNFNQPISGGNAFIHTAHLHQKAVKRDPSSYSWILPEYVGKTMELSGKSIPSVH
SLFLTPIVKCASELGYHRYGDSVGHLMIDECMVPNCRQSCIVREIRVVEEKPLAYVDVQTLYVDSLFLFLGHELGMKGLQ
VEVCLGQVVQKIDSPASVFIPAGVAHTYRILNGSGFCINHVLSGSYNDSLLEPLDISSNSYS

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=323, Percent_Identity=32.1981424148607, Blast_Score=132, Evalue=6e-32,
Organism=Saccharomyces cerevisiae, GI6320071, Length=308, Percent_Identity=33.7662337662338, Blast_Score=162, Evalue=8e-41,
Organism=Saccharomyces cerevisiae, GI6320019, Length=308, Percent_Identity=33.4415584415584, Blast_Score=161, Evalue=2e-40,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR011008
- InterPro:   IPR011872
- InterPro:   IPR000891
- InterPro:   IPR019887 [H]

Pfam domain/function: PF01037 AsnC_trans_reg; PF00682 HMGL-like [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 50825; Mature: 50825

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQIVDSTLREGRQTAPGINFSWEQSLKIAKLLHSLGIDIIEVCHPVVSIEQKQIVREIV
CCEEEHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
ASNLCPVIAHARANLADIEAVKETSAQWVGIFLGINEETRLTRARWTISKIFNCIKESVM
HCCCCHHHHHHHCCHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
YAKTLGLKVRYTAEDASRTDYCLLKSVYSVAIEAGADCICFSDTVGIMEPNEVTETIEKL
HHHHHCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHH
KSDFSTVPLEVHFHNDRGLAMANALAASDAGAERISSSVNGLGERCGITDTLGLLVNLYY
HHCCCCCCEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
KGLRSLPSGEIIQRTSKYVAAFSRYPVNFNQPISGGNAFIHTAHLHQKAVKRDPSSYSWI
HHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEHHHHHHHHHCCCCCCEEE
LPEYVGKTMELSGKSIPSVHSLFLTPIVKCASELGYHRYGDSVGHLMIDECMVPNCRQSC
CHHHCCCCEECCCCCCCHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHCCCCHHHHH
IVREIRVVEEKPLAYVDVQTLYVDSLFLFLGHELGMKGLQVEVCLGQVVQKIDSPASVFI
HHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEE
PAGVAHTYRILNGSGFCINHVLSGSYNDSLLEPLDISSNSYS
ECCHHHHEEEECCCCCEEHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MVQIVDSTLREGRQTAPGINFSWEQSLKIAKLLHSLGIDIIEVCHPVVSIEQKQIVREIV
CCEEEHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
ASNLCPVIAHARANLADIEAVKETSAQWVGIFLGINEETRLTRARWTISKIFNCIKESVM
HCCCCHHHHHHHCCHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
YAKTLGLKVRYTAEDASRTDYCLLKSVYSVAIEAGADCICFSDTVGIMEPNEVTETIEKL
HHHHHCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHH
KSDFSTVPLEVHFHNDRGLAMANALAASDAGAERISSSVNGLGERCGITDTLGLLVNLYY
HHCCCCCCEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
KGLRSLPSGEIIQRTSKYVAAFSRYPVNFNQPISGGNAFIHTAHLHQKAVKRDPSSYSWI
HHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEHHHHHHHHHCCCCCCEEE
LPEYVGKTMELSGKSIPSVHSLFLTPIVKCASELGYHRYGDSVGHLMIDECMVPNCRQSC
CHHHCCCCEECCCCCCCHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHCCCCHHHHH
IVREIRVVEEKPLAYVDVQTLYVDSLFLFLGHELGMKGLQVEVCLGQVVQKIDSPASVFI
HHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEE
PAGVAHTYRILNGSGFCINHVLSGSYNDSLLEPLDISSNSYS
ECCHHHHEEEECCCCCEEHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11572479 [H]