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The map label for this gene is exoA [H]

Identifier: 113477060

GI number: 113477060

Start: 5480746

End: 5481531

Strand: Reverse

Name: exoA [H]

Synonym: Tery_3564

Alternate gene names: 113477060

Gene position: 5481531-5480746 (Counterclockwise)

Preceding gene: 113477061

Following gene: 113477059

Centisome position: 70.73

GC content: 36.01

Gene sequence:

>786_bases
ATGAAAATAGCTACTTGGAATGTAAATTCAATTCGCACTCGTAAACAACAAGTGATAGAATGGCTAAAAGCAAACTCTGT
TGACATTCTCTGTTTGCAAGAAACTAAGGTTATTGACCAAGATTTTCCGCGATCGCCTTTTGCAGAATTAGGCTACCATA
TTTATGTTTCTGGTCAGAAATCTTACAATGGCGTGGCAATTTTTAGTCGTTCCCCAATGGAAGATATTAGTAGTGGTTTT
GCACCTATTTTAGGTGATCATAAAGTAGGAGATTTTGACATACAGAAACGGTTAATTACTGGGGTAATAAATGATGTTCG
TATTCTTAATCTTTATGTTCCCAATGGTTCTAGTGTCGGTAGTGAAAAATATGATTACAAACTTGATTGGTTAAAATTGT
TGCAAGAATATGTAAAAATAATTCTTGACAAATCGCCTAATTTATGTATTTGTGGAGATTTTAATATTGTCCCAGAAGAC
CGGGATATTTACGACCCAGAAGGTTGTAAAAATTCTGTAGGATTATCTGATCTCGAACGCCAAGCTTTACAAGAAATTCT
CAAATTAGGATTAGTCGATGCCTTTCGCCAATTTACCACCGAAGGAGGTCATTACAGTTGGTGGGATTATAGAGCAGCAG
GTTTTCGCCGTAATCGTGGTTGGCGTATTGACCATCATTATCTTAGTTTACCCCTTTCTCAAAAAGCAACAATGTGTGTA
ATAGATAAGAATCCAAGAGAGTTACTAAAACCTAGTGACCATGCACCAGTTATTGTAGAATTTTGA

Upstream 100 bases:

>100_bases
ACTTAGACAACTTTCTCAAGAAACTCACCAACAGTTAATCACTTGTAATCACAGAAAATTATCACTACTATTACTCTTGT
GACTTCTAATACAAACCATA

Downstream 100 bases:

>100_bases
AAAAATGAAGAAGGAAGAAGGAAGAAGGAAGAAGGAAGCAATAATCTAGTAGTTATAGTAACTATAGGAAAAAGCAGCCT
CAATATCTCACTATTTAGAT

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MKIATWNVNSIRTRKQQVIEWLKANSVDILCLQETKVIDQDFPRSPFAELGYHIYVSGQKSYNGVAIFSRSPMEDISSGF
APILGDHKVGDFDIQKRLITGVINDVRILNLYVPNGSSVGSEKYDYKLDWLKLLQEYVKIILDKSPNLCICGDFNIVPED
RDIYDPEGCKNSVGLSDLERQALQEILKLGLVDAFRQFTTEGGHYSWWDYRAAGFRRNRGWRIDHHYLSLPLSQKATMCV
IDKNPRELLKPSDHAPVIVEF

Sequences:

>Translated_261_residues
MKIATWNVNSIRTRKQQVIEWLKANSVDILCLQETKVIDQDFPRSPFAELGYHIYVSGQKSYNGVAIFSRSPMEDISSGF
APILGDHKVGDFDIQKRLITGVINDVRILNLYVPNGSSVGSEKYDYKLDWLKLLQEYVKIILDKSPNLCICGDFNIVPED
RDIYDPEGCKNSVGLSDLERQALQEILKLGLVDAFRQFTTEGGHYSWWDYRAAGFRRNRGWRIDHHYLSLPLSQKATMCV
IDKNPRELLKPSDHAPVIVEF
>Mature_261_residues
MKIATWNVNSIRTRKQQVIEWLKANSVDILCLQETKVIDQDFPRSPFAELGYHIYVSGQKSYNGVAIFSRSPMEDISSGF
APILGDHKVGDFDIQKRLITGVINDVRILNLYVPNGSSVGSEKYDYKLDWLKLLQEYVKIILDKSPNLCICGDFNIVPED
RDIYDPEGCKNSVGLSDLERQALQEILKLGLVDAFRQFTTEGGHYSWWDYRAAGFRRNRGWRIDHHYLSLPLSQKATMCV
IDKNPRELLKPSDHAPVIVEF

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=266, Percent_Identity=30.0751879699248, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI18375503, Length=266, Percent_Identity=30.0751879699248, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI18375501, Length=266, Percent_Identity=30.0751879699248, Blast_Score=122, Evalue=5e-28,
Organism=Escherichia coli, GI1788046, Length=273, Percent_Identity=34.0659340659341, Blast_Score=144, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI71989536, Length=271, Percent_Identity=30.6273062730627, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI221330655, Length=266, Percent_Identity=32.7067669172932, Blast_Score=121, Evalue=4e-28,
Organism=Drosophila melanogaster, GI17136678, Length=266, Percent_Identity=32.3308270676692, Blast_Score=120, Evalue=8e-28,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29912; Mature: 29912

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIATWNVNSIRTRKQQVIEWLKANSVDILCLQETKVIDQDFPRSPFAELGYHIYVSGQK
CEEEEECCHHHHHHHHHHHHHHHCCCEEEEEECCCHHHCCCCCCCCHHHCCEEEEEECCC
SYNGVAIFSRSPMEDISSGFAPILGDHKVGDFDIQKRLITGVINDVRILNLYVPNGSSVG
CCCCEEEEECCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCC
SEKYDYKLDWLKLLQEYVKIILDKSPNLCICGDFNIVPEDRDIYDPEGCKNSVGLSDLER
CCCCCEEHHHHHHHHHHHHHHHCCCCCEEEEECCEECCCCCCCCCCCCCCCCCCHHHHHH
QALQEILKLGLVDAFRQFTTEGGHYSWWDYRAAGFRRNRGWRIDHHYLSLPLSQKATMCV
HHHHHHHHHHHHHHHHHHHCCCCCEEEECHHCCCCCCCCCCEEEEEEEECCCCCCCEEEE
IDKNPRELLKPSDHAPVIVEF
ECCCHHHHCCCCCCCCEEEEC
>Mature Secondary Structure
MKIATWNVNSIRTRKQQVIEWLKANSVDILCLQETKVIDQDFPRSPFAELGYHIYVSGQK
CEEEEECCHHHHHHHHHHHHHHHCCCEEEEEECCCHHHCCCCCCCCHHHCCEEEEEECCC
SYNGVAIFSRSPMEDISSGFAPILGDHKVGDFDIQKRLITGVINDVRILNLYVPNGSSVG
CCCCEEEEECCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCC
SEKYDYKLDWLKLLQEYVKIILDKSPNLCICGDFNIVPEDRDIYDPEGCKNSVGLSDLER
CCCCCEEHHHHHHHHHHHHHHHCCCCCEEEEECCEECCCCCCCCCCCCCCCCCCHHHHHH
QALQEILKLGLVDAFRQFTTEGGHYSWWDYRAAGFRRNRGWRIDHHYLSLPLSQKATMCV
HHHHHHHHHHHHHHHHHHHCCCCCEEEECHHCCCCCCCCCCEEEEEEEECCCCCCCEEEE
IDKNPRELLKPSDHAPVIVEF
ECCCHHHHCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]