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The map label for this gene is ndhK

Identifier: 113477002

GI number: 113477002

Start: 5401277

End: 5401999

Strand: Reverse

Name: ndhK

Synonym: Tery_3500

Alternate gene names: 113477002

Gene position: 5401999-5401277 (Counterclockwise)

Preceding gene: 113477003

Following gene: 113477001

Centisome position: 69.7

GC content: 40.66

Gene sequence:

>723_bases
ATGGTCTTAAGTCCTAATCAAAACCAAAAAATTATTAATCCTATTGAGGAATCTCCGACAGTTACTCAAGAACTTTCAGA
GAATATAGTTTTAACTACAGTTGACGATCTTTATAACTGGGCCAAACTATCTAGTTTATGGCCTTTATTATATGGTACAG
CCTGTTGCTTTATTGAATTTGCAGCTCTCATCGGTTCCAGATTTGACTTTGACCGCTTTGGCTTAGTTCCACGGTCTAGC
CCTAGGCAAGCGGACTTACTAATTACTGCTGGTACAATCACCATGAAGTACACTCCTACCCTAGTACGTCTTTATGAACA
AATGCCAGAACCTAAATATGTCATTGCCATGGGTGCTTGTACAATTACAGGTGGAATGTTCAGCATGGACTCACCAACAG
CAGTTCGTGGGGTAGACAAATTAATTCCAGTGGATGTTTACATTCCTGGCTGCCCACCACGTCCAGAAGCAATTATTGAT
GCTGTCATTAAGCTGAGGAAAAAAATTGCTAATGATTCCTTGCAAGAAAGGGGTCAACTACAGCAAACACATCGTTACTA
TTGTGTTAAGCACAATATGAAAGTTGTTGAACCAATACTAACAGGTCAGTATCTACAAACATCTGACCGTAAAGCAGTGC
CAAAAGAGCTAGCTGAAGCAATAGGTATGCCAGTATCACCTGCACTGCAAGCTATACCAAAAGAGGAGGTAAGTCTTGGC
TGA

Upstream 100 bases:

>100_bases
CCTTTCATCAGTTGGGACTGTTAGCCTTTATAGAAGCACTGATTTTCATCGCAATCTTAGTGGTTGCTCTAGTATACGCA
TGGCGCAAAGGAGCTTTAGA

Downstream 100 bases:

>100_bases
ATCTGAAGCTCCAATTATTGAAGCCGGAAAAGTTTCAAAATGGCTCACAGACAATGGCTTTGATCATGAATTTCTAGCAC
CTGATCATCAGGGAATAGAA

Product: NADH dehydrogenase subunit B

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I subunit K; NDH-1 subunit K; NDH-K

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MVLSPNQNQKIINPIEESPTVTQELSENIVLTTVDDLYNWAKLSSLWPLLYGTACCFIEFAALIGSRFDFDRFGLVPRSS
PRQADLLITAGTITMKYTPTLVRLYEQMPEPKYVIAMGACTITGGMFSMDSPTAVRGVDKLIPVDVYIPGCPPRPEAIID
AVIKLRKKIANDSLQERGQLQQTHRYYCVKHNMKVVEPILTGQYLQTSDRKAVPKELAEAIGMPVSPALQAIPKEEVSLG

Sequences:

>Translated_240_residues
MVLSPNQNQKIINPIEESPTVTQELSENIVLTTVDDLYNWAKLSSLWPLLYGTACCFIEFAALIGSRFDFDRFGLVPRSS
PRQADLLITAGTITMKYTPTLVRLYEQMPEPKYVIAMGACTITGGMFSMDSPTAVRGVDKLIPVDVYIPGCPPRPEAIID
AVIKLRKKIANDSLQERGQLQQTHRYYCVKHNMKVVEPILTGQYLQTSDRKAVPKELAEAIGMPVSPALQAIPKEEVSLG
>Mature_240_residues
MVLSPNQNQKIINPIEESPTVTQELSENIVLTTVDDLYNWAKLSSLWPLLYGTACCFIEFAALIGSRFDFDRFGLVPRSS
PRQADLLITAGTITMKYTPTLVRLYEQMPEPKYVIAMGACTITGGMFSMDSPTAVRGVDKLIPVDVYIPGCPPRPEAIID
AVIKLRKKIANDSLQERGQLQQTHRYYCVKHNMKVVEPILTGQYLQTSDRKAVPKELAEAIGMPVSPALQAIPKEEVSLG

Specific function: NDH-1 shuttles electrons from an unknown electron donor, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory and/or the photosynthetic chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cellular thylakoid membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=143, Percent_Identity=49.6503496503497, Blast_Score=169, Evalue=2e-42,
Organism=Escherichia coli, GI1788624, Length=170, Percent_Identity=46.4705882352941, Blast_Score=164, Evalue=5e-42,
Organism=Escherichia coli, GI1788834, Length=151, Percent_Identity=36.4238410596026, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1789074, Length=108, Percent_Identity=42.5925925925926, Blast_Score=92, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17509685, Length=152, Percent_Identity=46.0526315789474, Blast_Score=158, Evalue=3e-39,
Organism=Drosophila melanogaster, GI18859983, Length=153, Percent_Identity=48.3660130718954, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24642371, Length=153, Percent_Identity=48.3660130718954, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24651058, Length=148, Percent_Identity=46.6216216216216, Blast_Score=162, Evalue=2e-40,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NDHK_TRIEI (Q10YT4)

Other databases:

- EMBL:   CP000393
- RefSeq:   YP_723063.1
- ProteinModelPortal:   Q10YT4
- SMR:   Q10YT4
- STRING:   Q10YT4
- GeneID:   4244325
- GenomeReviews:   CP000393_GR
- KEGG:   ter:Tery_3500
- NMPDR:   fig|203124.1.peg.1655
- eggNOG:   COG0377
- HOGENOM:   HBG553221
- OMA:   SENIVLT
- PhylomeDB:   Q10YT4
- ProtClustDB:   PRK06411
- BioCyc:   TERY203124:TERY_3500-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: 1.6.5.3

Molecular weight: Translated: 26642; Mature: 26642

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLSPNQNQKIINPIEESPTVTQELSENIVLTTVDDLYNWAKLSSLWPLLYGTACCFIEF
CCCCCCCCCCCCCCCCCCCCHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
AALIGSRFDFDRFGLVPRSSPRQADLLITAGTITMKYTPTLVRLYEQMPEPKYVIAMGAC
HHHHCCCCCHHHHCCCCCCCCCCCEEEEEECEEEEEECHHHHHHHHHCCCCCEEEEECCC
TITGGMFSMDSPTAVRGVDKLIPVDVYIPGCPPRPEAIIDAVIKLRKKIANDSLQERGQL
EEECCEECCCCCHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHH
QQTHRYYCVKHNMKVVEPILTGQYLQTSDRKAVPKELAEAIGMPVSPALQAIPKEEVSLG
HHHHHEEEEECCCHHHHHHHCCCHHCCCCCCCHHHHHHHHHCCCCCHHHHHCCCHHCCCC
>Mature Secondary Structure
MVLSPNQNQKIINPIEESPTVTQELSENIVLTTVDDLYNWAKLSSLWPLLYGTACCFIEF
CCCCCCCCCCCCCCCCCCCCHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
AALIGSRFDFDRFGLVPRSSPRQADLLITAGTITMKYTPTLVRLYEQMPEPKYVIAMGAC
HHHHCCCCCHHHHCCCCCCCCCCCEEEEEECEEEEEECHHHHHHHHHCCCCCEEEEECCC
TITGGMFSMDSPTAVRGVDKLIPVDVYIPGCPPRPEAIIDAVIKLRKKIANDSLQERGQL
EEECCEECCCCCHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHH
QQTHRYYCVKHNMKVVEPILTGQYLQTSDRKAVPKELAEAIGMPVSPALQAIPKEEVSLG
HHHHHEEEEECCCHHHHHHHCCCHHCCCCCCCHHHHHHHHHCCCCCHHHHHCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA