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The map label for this gene is lipA2 [H]

Identifier: 113476995

GI number: 113476995

Start: 5384892

End: 5385815

Strand: Reverse

Name: lipA2 [H]

Synonym: Tery_3493

Alternate gene names: 113476995

Gene position: 5385815-5384892 (Counterclockwise)

Preceding gene: 113476998

Following gene: 113476994

Centisome position: 69.49

GC content: 43.83

Gene sequence:

>924_bases
ATGGTCAATTCAATTAAGATGAAAGCCCAAATTCAGAGTATGCCAAAGTGGCTGCGCCCCTCTTTGGGTAAAGCTAGTGA
GATTTCTACTGTACAAAAAACTATCAAACAGCGTCAGCTCCATACAATTTGTGAGGAAGGTCGCTGTCCTAACCGAGGAG
AATGCTATGCCCAAAAAACTGCTACTTTTTTGTTAATGGGTCCTACCTGTACTAGGTCTTGTGCTTTTTGTCAAGTAGAT
AAGGGTCACGCTCCAATGGCTTTGGACCCAGAAGAACCGCAAAAAGTAGCGGAAGCAGTGGAATTATTGGGTTTGAAGTA
TGTAGTACTGACTTCTGTAGCTAGAGATGATCTCTCTGATGGTGGTGCTAGTTGGTTTGTAGCTACAATGATGGCTATTA
GGGATCGGAAGCCAGAGGCGAAAATAGAGGTTTTGACTGCTGACTTTTGGGGTGGTCAGAAGGTCAGGGGGGCTGAAAAG
TTATCTGTTCCTCTAGCTCAAAGACAAAGAAAACGGATTGAGATTGTGGTAAATGCTGAACCAGCTTGTTATAATCATAA
TATTGAAACGGTTTCTAGGTTGCAAGGGCCTGTACGTCGTGGGGCCAAATATCAGCGATCGCTTGATGTATTGCGAATTG
TTAAAGAGTTAAACCCCAGTATTCCTACTAAGTCTGGTTTGATGTTGGGACATGGAGAAAAGGAAACTGAAGTGGTAGAA
GCAATGGGAGACTTGAGAAAGGTAGGATGCGATCGCATAACTCTGGGTCAGTATATGCGACCTTCCCTGGCACATTTACC
AGTACAAAAATATTGGACACCAGAAGAATTTGATCATCTTGGGGCGATCGCTCTTTCAATGGGCTTTTCTCATGTTCGTT
CTGGCCCCTTAGTTCGTAGCTCCTATCATGCTGGAGAGATATAG

Upstream 100 bases:

>100_bases
TCTTGCAGATAAGTGCACCAGAGGCAGAAAGAAAACTGAGAAATTTTCTATTTATTTTCACGATTACCTAACTTCGGAAA
AATGTCAAGGCTACAAAAAA

Downstream 100 bases:

>100_bases
TATTTAAGATTATTTGGGAATAACTTCCAGAATATTCTGCCTCTATTCACAAGACATATACTGCCATTCATCTAATAACC
AAGGACAATAAATTTGGGAC

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn 2; Lipoate synthase 2; Lipoic acid synthase 2; Sulfur insertion protein lipA2 [H]

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MVNSIKMKAQIQSMPKWLRPSLGKASEISTVQKTIKQRQLHTICEEGRCPNRGECYAQKTATFLLMGPTCTRSCAFCQVD
KGHAPMALDPEEPQKVAEAVELLGLKYVVLTSVARDDLSDGGASWFVATMMAIRDRKPEAKIEVLTADFWGGQKVRGAEK
LSVPLAQRQRKRIEIVVNAEPACYNHNIETVSRLQGPVRRGAKYQRSLDVLRIVKELNPSIPTKSGLMLGHGEKETEVVE
AMGDLRKVGCDRITLGQYMRPSLAHLPVQKYWTPEEFDHLGAIALSMGFSHVRSGPLVRSSYHAGEI

Sequences:

>Translated_307_residues
MVNSIKMKAQIQSMPKWLRPSLGKASEISTVQKTIKQRQLHTICEEGRCPNRGECYAQKTATFLLMGPTCTRSCAFCQVD
KGHAPMALDPEEPQKVAEAVELLGLKYVVLTSVARDDLSDGGASWFVATMMAIRDRKPEAKIEVLTADFWGGQKVRGAEK
LSVPLAQRQRKRIEIVVNAEPACYNHNIETVSRLQGPVRRGAKYQRSLDVLRIVKELNPSIPTKSGLMLGHGEKETEVVE
AMGDLRKVGCDRITLGQYMRPSLAHLPVQKYWTPEEFDHLGAIALSMGFSHVRSGPLVRSSYHAGEI
>Mature_307_residues
MVNSIKMKAQIQSMPKWLRPSLGKASEISTVQKTIKQRQLHTICEEGRCPNRGECYAQKTATFLLMGPTCTRSCAFCQVD
KGHAPMALDPEEPQKVAEAVELLGLKYVVLTSVARDDLSDGGASWFVATMMAIRDRKPEAKIEVLTADFWGGQKVRGAEK
LSVPLAQRQRKRIEIVVNAEPACYNHNIETVSRLQGPVRRGAKYQRSLDVLRIVKELNPSIPTKSGLMLGHGEKETEVVE
AMGDLRKVGCDRITLGQYMRPSLAHLPVQKYWTPEEFDHLGAIALSMGFSHVRSGPLVRSSYHAGEI

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=311, Percent_Identity=41.1575562700965, Blast_Score=237, Evalue=1e-62,
Organism=Homo sapiens, GI37577164, Length=273, Percent_Identity=39.9267399267399, Blast_Score=196, Evalue=2e-50,
Organism=Escherichia coli, GI1786846, Length=291, Percent_Identity=45.360824742268, Blast_Score=270, Evalue=7e-74,
Organism=Caenorhabditis elegans, GI32564533, Length=303, Percent_Identity=44.2244224422442, Blast_Score=232, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6324770, Length=303, Percent_Identity=41.9141914191419, Blast_Score=244, Evalue=1e-65,
Organism=Drosophila melanogaster, GI221513272, Length=308, Percent_Identity=40.5844155844156, Blast_Score=230, Evalue=1e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 34125; Mature: 34125

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNSIKMKAQIQSMPKWLRPSLGKASEISTVQKTIKQRQLHTICEEGRCPNRGECYAQKT
CCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCC
ATFLLMGPTCTRSCAFCQVDKGHAPMALDPEEPQKVAEAVELLGLKYVVLTSVARDDLSD
EEEEEECCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GGASWFVATMMAIRDRKPEAKIEVLTADFWGGQKVRGAEKLSVPLAQRQRKRIEIVVNAE
CCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCHHHHCCCHHHHCCCEEEEEEECC
PACYNHNIETVSRLQGPVRRGAKYQRSLDVLRIVKELNPSIPTKSGLMLGHGEKETEVVE
CCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHH
AMGDLRKVGCDRITLGQYMRPSLAHLPVQKYWTPEEFDHLGAIALSMGFSHVRSGPLVRS
HHHHHHHHCCCCEEHHHHHCCHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEC
SYHAGEI
CCCCCCC
>Mature Secondary Structure
MVNSIKMKAQIQSMPKWLRPSLGKASEISTVQKTIKQRQLHTICEEGRCPNRGECYAQKT
CCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCC
ATFLLMGPTCTRSCAFCQVDKGHAPMALDPEEPQKVAEAVELLGLKYVVLTSVARDDLSD
EEEEEECCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GGASWFVATMMAIRDRKPEAKIEVLTADFWGGQKVRGAEKLSVPLAQRQRKRIEIVVNAE
CCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCHHHHCCCHHHHCCCEEEEEEECC
PACYNHNIETVSRLQGPVRRGAKYQRSLDVLRIVKELNPSIPTKSGLMLGHGEKETEVVE
CCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHH
AMGDLRKVGCDRITLGQYMRPSLAHLPVQKYWTPEEFDHLGAIALSMGFSHVRSGPLVRS
HHHHHHHHCCCCEEHHHHHCCHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEC
SYHAGEI
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]