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The map label for this gene is gloA [H]

Identifier: 113476993

GI number: 113476993

Start: 5382588

End: 5383016

Strand: Reverse

Name: gloA [H]

Synonym: Tery_3491

Alternate gene names: 113476993

Gene position: 5383016-5382588 (Counterclockwise)

Preceding gene: 113476994

Following gene: 113476992

Centisome position: 69.46

GC content: 37.3

Gene sequence:

>429_bases
ATGCGACTCTTACACACCATGCTGCGAGTCAATAACTTAGAAAAATCTATAGAATTCTACTGTGATGTCCTCGGCATGAA
ACTACTACGTAAAAAAGATTTCCCTGGTGGCAAATTTACTCTAGCTTTCGTTGGTTATGGCGATGAGTTAAACCATACAG
TATTAGAACTGACCTATAACTGGGACACTGATAAATACGATTTAGGCAATGCTTATGGCCATATTGCCTTGGGTGTTGAT
GATATTTATAGCACTTGCGAAAAAATTAAAGAACAAGGGGGTAAAGTTACTAGGGAACCAGGGCCAATGAAGCATGGTTC
TACAGTTATTGCTTTTATAGAAGACCCAAATGGATATAAAGTAGAGCTAATTGAGTTAAAGAGTGACAACAGCAGCTTAG
AAAAAAAAGTTGCAGCTAATACTAATTGA

Upstream 100 bases:

>100_bases
AGAATGGTTATTCCCTCCTATTGACTTATGCTATATCTACTCCCAATATTAACCAAATTGCCTCACAATATAAATATAGT
CAAAGAATAAGGAGTAAACC

Downstream 100 bases:

>100_bases
AAAGTTAGATATTGCTGAGGAGTAGAATGAATGATAAGTGGGGTAGTTACCCTATAACTAAAATCAAAATTCAGAACTCA
TAAATCAAAACTTAAACTCG

Product: lactoylglutathione lyase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 142; Mature: 142

Protein sequence:

>142_residues
MRLLHTMLRVNNLEKSIEFYCDVLGMKLLRKKDFPGGKFTLAFVGYGDELNHTVLELTYNWDTDKYDLGNAYGHIALGVD
DIYSTCEKIKEQGGKVTREPGPMKHGSTVIAFIEDPNGYKVELIELKSDNSSLEKKVAANTN

Sequences:

>Translated_142_residues
MRLLHTMLRVNNLEKSIEFYCDVLGMKLLRKKDFPGGKFTLAFVGYGDELNHTVLELTYNWDTDKYDLGNAYGHIALGVD
DIYSTCEKIKEQGGKVTREPGPMKHGSTVIAFIEDPNGYKVELIELKSDNSSLEKKVAANTN
>Mature_142_residues
MRLLHTMLRVNNLEKSIEFYCDVLGMKLLRKKDFPGGKFTLAFVGYGDELNHTVLELTYNWDTDKYDLGNAYGHIALGVD
DIYSTCEKIKEQGGKVTREPGPMKHGSTVIAFIEDPNGYKVELIELKSDNSSLEKKVAANTN

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=148, Percent_Identity=39.1891891891892, Blast_Score=97, Evalue=4e-21,
Organism=Homo sapiens, GI217330598, Length=133, Percent_Identity=33.0827067669173, Blast_Score=67, Evalue=6e-12,
Organism=Escherichia coli, GI1787940, Length=133, Percent_Identity=63.9097744360902, Blast_Score=181, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17552228, Length=151, Percent_Identity=32.4503311258278, Blast_Score=77, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323639, Length=139, Percent_Identity=40.2877697841727, Blast_Score=95, Evalue=5e-21,
Organism=Drosophila melanogaster, GI20129743, Length=150, Percent_Identity=36, Blast_Score=90, Evalue=4e-19,
Organism=Drosophila melanogaster, GI19920404, Length=152, Percent_Identity=30.9210526315789, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 15991; Mature: 15991

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00934 GLYOXALASE_I_1 ; PS00935 GLYOXALASE_I_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLLHTMLRVNNLEKSIEFYCDVLGMKLLRKKDFPGGKFTLAFVGYGDELNHTVLELTYN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCEEEEEEEC
WDTDKYDLGNAYGHIALGVDDIYSTCEKIKEQGGKVTREPGPMKHGSTVIAFIEDPNGYK
CCCCCEECCCCCEEEEECHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCE
VELIELKSDNSSLEKKVAANTN
EEEEEEECCCCHHHHHHCCCCC
>Mature Secondary Structure
MRLLHTMLRVNNLEKSIEFYCDVLGMKLLRKKDFPGGKFTLAFVGYGDELNHTVLELTYN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCEEEEEEEC
WDTDKYDLGNAYGHIALGVDDIYSTCEKIKEQGGKVTREPGPMKHGSTVIAFIEDPNGYK
CCCCCEECCCCCEEEEECHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCE
VELIELKSDNSSLEKKVAANTN
EEEEEEECCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]