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The map label for this gene is gca [H]

Identifier: 113476521

GI number: 113476521

Start: 4577714

End: 4578703

Strand: Reverse

Name: gca [H]

Synonym: Tery_2945

Alternate gene names: 113476521

Gene position: 4578703-4577714 (Counterclockwise)

Preceding gene: 113476522

Following gene: 113476520

Centisome position: 59.08

GC content: 32.73

Gene sequence:

>990_bases
ATGAAAACAGCATTAATTTCAGGAATTTCCGGTCAAGATGGTTCCTACTTAGCTAAATTGTTACTCGAAAAAGGTTACAA
TGTTTGTGGCACTTCCAGAGATGCTCAAATGTCTACCTTTAAAAACTTATCTTATTTAGGTATTCGTGAACAGATAAAGT
TAGATTCTATGTCTTTAAATGACTTTAGAAGTGTCTTACAAATATTAACCAAATATCAACCAGATGAAATTTATAATCTA
GCTGGTCAAAGTTCAGTAGGTTTATCTTTTGAGCAACCTGTAGAAACACTAGAAAGTATCGCTACTGGTACATTGAATTT
ATTAGAAGCAATTCGTTTTATTGGAGAAAAAATTAAACTATACAATGCTAGTTCCAGTGAATGTTTTGGGGATACAGAAG
GTCAAACTGCGACAGAGATTACTCCATTTCGCCCTAGAAGTCCTTACGCTGTTGCTAAATCTGCAGCTTTTTGGGAAGTA
GCAAATTATCGAGAAGCTTATAATTTATTTGCTTGTTCTGGAATTTTATTTAATCACGAATCTCCTCTCAGACCTCAACG
TTTTGTTACTCAAAAAATTGTCTCAGTTGTTTCTAGAATTGCTCAAGGTAGTAAAGAGAAATTATCTTTAGGAAATATTT
CAGTACAAAGAGATTGGGGTTGGGCACCAGAATATGTAAAAGCAATGTTTTTAATGTTACAGCAAGATAAACCAGATGAT
TATATAATTGCTACTGGAAAAACTTATTCTTTGCAGGATTTTGTATTAGAAGCTTTTGCCGCTGTAGGATTAAATTGGCA
GGATTATGTAGAGATAGATAAGGGTTTATATCGTCCTACTGATATTTTTGTTAGTAAGGCAAATCCAATTAAAGCTGAAC
AAAAATTAGGATGGAAAGCAGATTATAAAATGCCAGATGTTGTGCGGATGATGCTTAAGGATAGAATGGCTAATTTAGAA
ATTATTGAAAGTCGTAAAAAAATAAAATAA

Upstream 100 bases:

>100_bases
CTTTATTAATGACAATTTAAATTAGGAGATTTTTTTTAGCAAAAAAAATCCCTAAACATTATCTCCTACTATTTACCTCT
TTTTTCCTTATTTTAAGATT

Downstream 100 bases:

>100_bases
TAAAGGCAAACAATCAGCATATTTTTAGTGATATGTAAAAAATAGATTGTTGTTAGTATACAATTTTTATTTTTCGTTAA
AATTAAGATAAAATATTTTA

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MKTALISGISGQDGSYLAKLLLEKGYNVCGTSRDAQMSTFKNLSYLGIREQIKLDSMSLNDFRSVLQILTKYQPDEIYNL
AGQSSVGLSFEQPVETLESIATGTLNLLEAIRFIGEKIKLYNASSSECFGDTEGQTATEITPFRPRSPYAVAKSAAFWEV
ANYREAYNLFACSGILFNHESPLRPQRFVTQKIVSVVSRIAQGSKEKLSLGNISVQRDWGWAPEYVKAMFLMLQQDKPDD
YIIATGKTYSLQDFVLEAFAAVGLNWQDYVEIDKGLYRPTDIFVSKANPIKAEQKLGWKADYKMPDVVRMMLKDRMANLE
IIESRKKIK

Sequences:

>Translated_329_residues
MKTALISGISGQDGSYLAKLLLEKGYNVCGTSRDAQMSTFKNLSYLGIREQIKLDSMSLNDFRSVLQILTKYQPDEIYNL
AGQSSVGLSFEQPVETLESIATGTLNLLEAIRFIGEKIKLYNASSSECFGDTEGQTATEITPFRPRSPYAVAKSAAFWEV
ANYREAYNLFACSGILFNHESPLRPQRFVTQKIVSVVSRIAQGSKEKLSLGNISVQRDWGWAPEYVKAMFLMLQQDKPDD
YIIATGKTYSLQDFVLEAFAAVGLNWQDYVEIDKGLYRPTDIFVSKANPIKAEQKLGWKADYKMPDVVRMMLKDRMANLE
IIESRKKIK
>Mature_329_residues
MKTALISGISGQDGSYLAKLLLEKGYNVCGTSRDAQMSTFKNLSYLGIREQIKLDSMSLNDFRSVLQILTKYQPDEIYNL
AGQSSVGLSFEQPVETLESIATGTLNLLEAIRFIGEKIKLYNASSSECFGDTEGQTATEITPFRPRSPYAVAKSAAFWEV
ANYREAYNLFACSGILFNHESPLRPQRFVTQKIVSVVSRIAQGSKEKLSLGNISVQRDWGWAPEYVKAMFLMLQQDKPDD
YIIATGKTYSLQDFVLEAFAAVGLNWQDYVEIDKGLYRPTDIFVSKANPIKAEQKLGWKADYKMPDVVRMMLKDRMANLE
IIESRKKIK

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=346, Percent_Identity=41.907514450867, Blast_Score=265, Evalue=3e-71,
Organism=Escherichia coli, GI1788366, Length=349, Percent_Identity=41.2607449856734, Blast_Score=270, Evalue=7e-74,
Organism=Escherichia coli, GI48994969, Length=262, Percent_Identity=24.0458015267176, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1788353, Length=274, Percent_Identity=23.3576642335766, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17507723, Length=344, Percent_Identity=43.8953488372093, Blast_Score=280, Evalue=6e-76,
Organism=Caenorhabditis elegans, GI17539424, Length=336, Percent_Identity=44.047619047619, Blast_Score=280, Evalue=9e-76,
Organism=Caenorhabditis elegans, GI133901788, Length=336, Percent_Identity=44.047619047619, Blast_Score=280, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI133901790, Length=336, Percent_Identity=44.047619047619, Blast_Score=280, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI17539422, Length=336, Percent_Identity=44.047619047619, Blast_Score=280, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI133901786, Length=336, Percent_Identity=44.047619047619, Blast_Score=279, Evalue=1e-75,
Organism=Drosophila melanogaster, GI24158427, Length=348, Percent_Identity=41.6666666666667, Blast_Score=259, Evalue=2e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 37079; Mature: 37079

Theoretical pI: Translated: 8.37; Mature: 8.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTALISGISGQDGSYLAKLLLEKGYNVCGTSRDAQMSTFKNLSYLGIREQIKLDSMSLN
CCCCEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCHHHCHHHHHEECCCCHH
DFRSVLQILTKYQPDEIYNLAGQSSVGLSFEQPVETLESIATGTLNLLEAIRFIGEKIKL
HHHHHHHHHHHCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
YNASSSECFGDTEGQTATEITPFRPRSPYAVAKSAAFWEVANYREAYNLFACSGILFNHE
ECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCC
SPLRPQRFVTQKIVSVVSRIAQGSKEKLSLGNISVQRDWGWAPEYVKAMFLMLQQDKPDD
CCCCHHHHHHHHHHHHHHHHHCCCCHHEEECCEEEECCCCCCHHHHHHHHHHHHCCCCCC
YIIATGKTYSLQDFVLEAFAAVGLNWQDYVEIDKGLYRPTDIFVSKANPIKAEQKLGWKA
EEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHEEEECCCCCCHHHHCCCCC
DYKMPDVVRMMLKDRMANLEIIESRKKIK
CCCCHHHHHHHHHHHHCCHHHHHHHHCCC
>Mature Secondary Structure
MKTALISGISGQDGSYLAKLLLEKGYNVCGTSRDAQMSTFKNLSYLGIREQIKLDSMSLN
CCCCEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCHHHCHHHHHEECCCCHH
DFRSVLQILTKYQPDEIYNLAGQSSVGLSFEQPVETLESIATGTLNLLEAIRFIGEKIKL
HHHHHHHHHHHCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
YNASSSECFGDTEGQTATEITPFRPRSPYAVAKSAAFWEVANYREAYNLFACSGILFNHE
ECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCC
SPLRPQRFVTQKIVSVVSRIAQGSKEKLSLGNISVQRDWGWAPEYVKAMFLMLQQDKPDD
CCCCHHHHHHHHHHHHHHHHHCCCCHHEEECCEEEECCCCCCHHHHHHHHHHHHCCCCCC
YIIATGKTYSLQDFVLEAFAAVGLNWQDYVEIDKGLYRPTDIFVSKANPIKAEQKLGWKA
EEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHEEEECCCCCCHHHHCCCCC
DYKMPDVVRMMLKDRMANLEIIESRKKIK
CCCCHHHHHHHHHHHHCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8548534; 10984043 [H]