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The map label for this gene is potB [H]

Identifier: 113476406

GI number: 113476406

Start: 4380083

End: 4380964

Strand: Reverse

Name: potB [H]

Synonym: Tery_2818

Alternate gene names: 113476406

Gene position: 4380964-4380083 (Counterclockwise)

Preceding gene: 113476408

Following gene: 113476405

Centisome position: 56.53

GC content: 32.43

Gene sequence:

>882_bases
ATGTGGCAACCTCAGAAAAAACAGTCAACCTCAAACATTGAAAAATCTCAACTAAAAAAATTACTAATTCTTCTATTCCC
TGCAACTATCTGGCTATTAATTTTTTTCATCATCCCGTTAGTAATTGTTTTAATTTACAGCTTCCTAGAACGAGGTACTT
ATGGCGGTGTCGTTTGGAAATTTACTCTAGATAACTATCAACGACTAGGTCAAGGAATATACTGGGGAATATTTTGGCGG
TCTTTATTCTTTAGTTTAACTACAACTATAATGTGTTTATTAATTGGCTACCCTTTAGCATATTTTATTGCTACTATTAG
ACCACCTTGGCGTAATGTTTTATTGATGTTAGTTATTATTCCTTTTTGGACTAATTTTTTAGTACGTACTTATGCTTGGA
TAGTCCTGCTTCGGACTGAAGGAATATTTAATATTGCCTTACAAAGTTTACAGCTAACAGACAAACCTTTAACTATTTTA
TTTACACCCTTTGCTGTGACTATTGGTTTAGTTTATGGCTACTTGCCGTTTATGATTTTACCTTTATATGCCACAATTGA
ACGCTTTAATTTTTATTTATTAGAAGCTGCTCAAGATTTGGGAGCTAATGATTTACGCACCTTTTTTCGTATATTTTTAC
CATTAACAATTCGAGGTATAGTTGCAGGTTGTTTACTAGTTTTTATTCCGGCATTTGGTGCTTTTATTACTCCTGATATT
TTAGGTGGTGCAAAAACTATGATGGTAGGAAACTTGATTCAAAATCAATTTATTAAAGCTAGAAATTGGCCGTTTGGTTC
GGCACTATCAATTGCCTTGATGATAATTGTATTAATTCCAGTTATCATCTATTTTGCAGTATCGGAAGAGGAAAATAATT
AA

Upstream 100 bases:

>100_bases
TCAAAAATTATTATACTGGCTTCAAAAAATACTACCAAAAATTATTGACAAATAAACATAAATATGATGGTCATAATTTC
TGACTTCTTGGCTAATTAAA

Downstream 100 bases:

>100_bases
GTATTCAGCTATTAGGAATAAGTAACAGATAATTACAAGTATATGATTGAAAAATATTAATAATAAAATATTTATAGCAC
AGGTATATTGCCTGTGATAT

Product: binding-protein-dependent transport systems inner membrane component

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MWQPQKKQSTSNIEKSQLKKLLILLFPATIWLLIFFIIPLVIVLIYSFLERGTYGGVVWKFTLDNYQRLGQGIYWGIFWR
SLFFSLTTTIMCLLIGYPLAYFIATIRPPWRNVLLMLVIIPFWTNFLVRTYAWIVLLRTEGIFNIALQSLQLTDKPLTIL
FTPFAVTIGLVYGYLPFMILPLYATIERFNFYLLEAAQDLGANDLRTFFRIFLPLTIRGIVAGCLLVFIPAFGAFITPDI
LGGAKTMMVGNLIQNQFIKARNWPFGSALSIALMIIVLIPVIIYFAVSEEENN

Sequences:

>Translated_293_residues
MWQPQKKQSTSNIEKSQLKKLLILLFPATIWLLIFFIIPLVIVLIYSFLERGTYGGVVWKFTLDNYQRLGQGIYWGIFWR
SLFFSLTTTIMCLLIGYPLAYFIATIRPPWRNVLLMLVIIPFWTNFLVRTYAWIVLLRTEGIFNIALQSLQLTDKPLTIL
FTPFAVTIGLVYGYLPFMILPLYATIERFNFYLLEAAQDLGANDLRTFFRIFLPLTIRGIVAGCLLVFIPAFGAFITPDI
LGGAKTMMVGNLIQNQFIKARNWPFGSALSIALMIIVLIPVIIYFAVSEEENN
>Mature_293_residues
MWQPQKKQSTSNIEKSQLKKLLILLFPATIWLLIFFIIPLVIVLIYSFLERGTYGGVVWKFTLDNYQRLGQGIYWGIFWR
SLFFSLTTTIMCLLIGYPLAYFIATIRPPWRNVLLMLVIIPFWTNFLVRTYAWIVLLRTEGIFNIALQSLQLTDKPLTIL
FTPFAVTIGLVYGYLPFMILPLYATIERFNFYLLEAAQDLGANDLRTFFRIFLPLTIRGIVAGCLLVFIPAFGAFITPDI
LGGAKTMMVGNLIQNQFIKARNWPFGSALSIALMIIVLIPVIIYFAVSEEENN

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=263, Percent_Identity=46.7680608365019, Blast_Score=238, Evalue=4e-64,
Organism=Escherichia coli, GI1787080, Length=248, Percent_Identity=42.741935483871, Blast_Score=211, Evalue=6e-56,
Organism=Escherichia coli, GI1787713, Length=231, Percent_Identity=32.4675324675325, Blast_Score=102, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 33637; Mature: 33637

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure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HCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]