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The map label for this gene is tgt [H]

Identifier: 113476080

GI number: 113476080

Start: 3780471

End: 3781574

Strand: Reverse

Name: tgt [H]

Synonym: Tery_2456

Alternate gene names: 113476080

Gene position: 3781574-3780471 (Counterclockwise)

Preceding gene: 113476081

Following gene: 113476070

Centisome position: 48.79

GC content: 41.85

Gene sequence:

>1104_bases
ATGTCCCAAAACTTTTCCTTTAATTCTCAGGCTCGTTGTAGCTATACAAAAGCTCGTGCAGGTATTTTTATAACTCCTCA
TGGGAGTATAAATACACCTAGATTTATGCCTGTGGGAACCTTGGCAAATGTAAAAACTCTCACACCTGCTCAGTTAGAGG
CGGCAGGTGCGCAGATGATCTTGGCAAATACTTATCATCTTCACTTACAACCAGGAGAAAGTATTGTTGCTGGTGCTGGT
GGGTTACATAAATTTATGGCTTGGAATAGGCCAATATTAACTGACTCTGGAGGATTTCAGGTATTTAGCTTAGGAGATAT
CCGAACTATTAGCGAAGCAGGAGTTAAGTTTCGCTCTCCTCATGATGGTAGCATGATTGATTTAACTCCAGAACGCTCGA
TCCAAATTCAGAATGAATTGGGAGGGGATGTAATTATGGCTTTTGATGAGTGTCCACCTTATCCAGCGAGTTGGGAAGAG
GTGGAGTTGGCAACAAATCGCACTTATCGATGGTTGCAGCGTTGCATAGAGGCTCATCAACGTCAGGATCAAGCTTTGTT
TGGTATTGTTCAAGGTGGTGTTTATCCTGAACTTCGTTCTATAGCAGCAAGGCAGTTGGTAGATTTGGATCTGCCTGGTT
ATGCTATTGGTGGGGTAAGTGTAGGAGAACCAGGCGAGTTAATAGATCATATTGTGAAGGTGACAACTCCTTTCTTGCCA
GAGGATAAGCCTCGTTATTTGATGGGAGTTGGCACTTATAGGGAAATGGTACGGGCGATCGCTGAGGGTATAGATTTATT
TGATTGTGTGATTCCTACTCGTTTGGGGCGACATGGGGCAGCATTAGTGAGGGGTGAGAGGTGGAATTTGAAGAATGCTA
GGTTTAAGGAAGATTTTAGACCACTTGATGAGTTTTGTCCTTGTTATTGTTGTCAAAATTTTAGTCGAGCTTATCTGAGT
CATTTGGTTAGAGCAAAGGAAGCTTTGGGTTATACTCTTTTGTCTTTGCATAATGTTACAGAATTGATTAGTTTTACCCA
ACGTATTCGGGATGCTATTTTGAAAGATAGGTTTATTACGGAGTTTGGTAGTTGGCTAAGATAG

Upstream 100 bases:

>100_bases
AAGGCGCGAAGTTAAAATCAAATTTTGGCTCTGACTAAATTTCTCATTCTAGAGAAAATAGGTGAAATTTTCTCGCTTAC
TAAAATCTAAGATCGATCTT

Downstream 100 bases:

>100_bases
TTGAAGTGAGATTCAAAACCATAATCAAAGACTTTGGAAATTACGGTTTGGTTTTAGCCTTGTCAAGTAGTAAAATTAAC
CACCAGGACTTACACAAAAA

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 367; Mature: 366

Protein sequence:

>367_residues
MSQNFSFNSQARCSYTKARAGIFITPHGSINTPRFMPVGTLANVKTLTPAQLEAAGAQMILANTYHLHLQPGESIVAGAG
GLHKFMAWNRPILTDSGGFQVFSLGDIRTISEAGVKFRSPHDGSMIDLTPERSIQIQNELGGDVIMAFDECPPYPASWEE
VELATNRTYRWLQRCIEAHQRQDQALFGIVQGGVYPELRSIAARQLVDLDLPGYAIGGVSVGEPGELIDHIVKVTTPFLP
EDKPRYLMGVGTYREMVRAIAEGIDLFDCVIPTRLGRHGAALVRGERWNLKNARFKEDFRPLDEFCPCYCCQNFSRAYLS
HLVRAKEALGYTLLSLHNVTELISFTQRIRDAILKDRFITEFGSWLR

Sequences:

>Translated_367_residues
MSQNFSFNSQARCSYTKARAGIFITPHGSINTPRFMPVGTLANVKTLTPAQLEAAGAQMILANTYHLHLQPGESIVAGAG
GLHKFMAWNRPILTDSGGFQVFSLGDIRTISEAGVKFRSPHDGSMIDLTPERSIQIQNELGGDVIMAFDECPPYPASWEE
VELATNRTYRWLQRCIEAHQRQDQALFGIVQGGVYPELRSIAARQLVDLDLPGYAIGGVSVGEPGELIDHIVKVTTPFLP
EDKPRYLMGVGTYREMVRAIAEGIDLFDCVIPTRLGRHGAALVRGERWNLKNARFKEDFRPLDEFCPCYCCQNFSRAYLS
HLVRAKEALGYTLLSLHNVTELISFTQRIRDAILKDRFITEFGSWLR
>Mature_366_residues
SQNFSFNSQARCSYTKARAGIFITPHGSINTPRFMPVGTLANVKTLTPAQLEAAGAQMILANTYHLHLQPGESIVAGAGG
LHKFMAWNRPILTDSGGFQVFSLGDIRTISEAGVKFRSPHDGSMIDLTPERSIQIQNELGGDVIMAFDECPPYPASWEEV
ELATNRTYRWLQRCIEAHQRQDQALFGIVQGGVYPELRSIAARQLVDLDLPGYAIGGVSVGEPGELIDHIVKVTTPFLPE
DKPRYLMGVGTYREMVRAIAEGIDLFDCVIPTRLGRHGAALVRGERWNLKNARFKEDFRPLDEFCPCYCCQNFSRAYLSH
LVRAKEALGYTLLSLHNVTELISFTQRIRDAILKDRFITEFGSWLR

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=349, Percent_Identity=45.8452722063037, Blast_Score=324, Evalue=9e-89,
Organism=Homo sapiens, GI13375872, Length=284, Percent_Identity=26.4084507042254, Blast_Score=105, Evalue=6e-23,
Organism=Escherichia coli, GI1786607, Length=351, Percent_Identity=48.4330484330484, Blast_Score=345, Evalue=3e-96,
Organism=Caenorhabditis elegans, GI17544680, Length=349, Percent_Identity=44.1260744985673, Blast_Score=296, Evalue=1e-80,
Organism=Caenorhabditis elegans, GI25146502, Length=234, Percent_Identity=24.7863247863248, Blast_Score=78, Evalue=8e-15,
Organism=Drosophila melanogaster, GI19920510, Length=355, Percent_Identity=47.3239436619718, Blast_Score=332, Evalue=2e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 41038; Mature: 40906

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQNFSFNSQARCSYTKARAGIFITPHGSINTPRFMPVGTLANVKTLTPAQLEAAGAQMI
CCCCCCCCCCCCEEEHHHCCCEEEECCCCCCCCCEEECCCCCCCCCCCCHHHHHCCCEEE
LANTYHLHLQPGESIVAGAGGLHKFMAWNRPILTDSGGFQVFSLGDIRTISEAGVKFRSP
EEEEEEEEECCCCCEEECCCHHHHHHHCCCCEEECCCCEEEEECCCCHHHHHCCCEECCC
HDGSMIDLTPERSIQIQNELGGDVIMAFDECPPYPASWEEVELATNRTYRWLQRCIEAHQ
CCCCEEEECCCCCEEECCCCCCCEEEEECCCCCCCCCCHHEEHHHHHHHHHHHHHHHHHH
RQDQALFGIVQGGVYPELRSIAARQLVDLDLPGYAIGGVSVGEPGELIDHIVKVTTPFLP
HHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHHHHCCCCC
EDKPRYLMGVGTYREMVRAIAEGIDLFDCVIPTRLGRHGAALVRGERWNLKNARFKEDFR
CCCCCEEEECHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCHHHHCC
PLDEFCPCYCCQNFSRAYLSHLVRAKEALGYTLLSLHNVTELISFTQRIRDAILKDRFIT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EFGSWLR
HHHHHCC
>Mature Secondary Structure 
SQNFSFNSQARCSYTKARAGIFITPHGSINTPRFMPVGTLANVKTLTPAQLEAAGAQMI
CCCCCCCCCCCEEEHHHCCCEEEECCCCCCCCCEEECCCCCCCCCCCCHHHHHCCCEEE
LANTYHLHLQPGESIVAGAGGLHKFMAWNRPILTDSGGFQVFSLGDIRTISEAGVKFRSP
EEEEEEEEECCCCCEEECCCHHHHHHHCCCCEEECCCCEEEEECCCCHHHHHCCCEECCC
HDGSMIDLTPERSIQIQNELGGDVIMAFDECPPYPASWEEVELATNRTYRWLQRCIEAHQ
CCCCEEEECCCCCEEECCCCCCCEEEEECCCCCCCCCCHHEEHHHHHHHHHHHHHHHHHH
RQDQALFGIVQGGVYPELRSIAARQLVDLDLPGYAIGGVSVGEPGELIDHIVKVTTPFLP
HHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHHHHCCCCC
EDKPRYLMGVGTYREMVRAIAEGIDLFDCVIPTRLGRHGAALVRGERWNLKNARFKEDFR
CCCCCEEEECHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCHHHHCC
PLDEFCPCYCCQNFSRAYLSHLVRAKEALGYTLLSLHNVTELISFTQRIRDAILKDRFIT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EFGSWLR
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]