Click here to switch to the map view.

The map label for this gene is hisH

Identifier: 113475789

GI number: 113475789

Start: 3346524

End: 3347168

Strand: Reverse

Name: hisH

Synonym: Tery_2143

Alternate gene names: 113475789

Gene position: 3347168-3346524 (Counterclockwise)

Preceding gene: 113475793

Following gene: 113475788

Centisome position: 43.19

GC content: 35.35

Gene sequence:

>645_bases
ATGTCAACTATTGCAGTTATAGATTACGATATGGGTAACTTGCACTCCGTTTGTAAAGGCTTAGAAAAAGCAGGAGCAAC
TCCAAAAATTACAGATTCTTCAATAGAAATAGACAAGGCTGATGCAGTGATTTTACCAGGGGTAGGGTCTTTCGATCCAG
CAGTACAACATTTAAGAACACGCAACTTAGAAATACCTATTAAACAAGTTATTGCTTCTGGAAAACCATTTTTAGGTATA
TGTTTAGGTTTACAAATATTATTTGAAAGTAGCGAAGAAGGTCAAGAACCAGGCTTAGGAATATTTGCTGGAAAAGTATG
TCGGTTTAAATCTGAACCTGGCCTGACAATTCCACAAATGGGTTGGAATAAATTACAATTTACTCAACCAGAACATCTTT
TGTGGAGTGAAATAGGTTCTCAACCCTGGGTATATTTTGTTCATTCTTATTATGTTGATCCTACAGACTCTCAAGTTACA
GCAGCAACTGTCACCCATGGTCATCAAACTGTTACTGCAGCAGTAGGAAAAGATAATTTAATAGCAGTACAATTTCACCC
AGAAAAATCTTCTACTGCCGGATTAAAAATTTTGTCAAATTTTGTTAGTAAAGTTATCCCTAAAAATTTGGCTTTAGCTA
GTTAA

Upstream 100 bases:

>100_bases
AATTATCACCAAATTATAAAAAGTGTAAATCCTTTCAATTATGGTAATTTTTTACATATAATATTAAAGTTACTAAATAT
AAAATTAGGATAATGAATAT

Downstream 100 bases:

>100_bases
GGCTTGCTGAAAAAGTCTGAGCAGTGAAGTTAGAACTCAGAAATAGGTGGGAACATTTCATGGAACTTACTTAGGTCTAA
ATGGGAATTAATTAGAAAGT

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH

Number of amino acids: Translated: 214; Mature: 213

Protein sequence:

>214_residues
MSTIAVIDYDMGNLHSVCKGLEKAGATPKITDSSIEIDKADAVILPGVGSFDPAVQHLRTRNLEIPIKQVIASGKPFLGI
CLGLQILFESSEEGQEPGLGIFAGKVCRFKSEPGLTIPQMGWNKLQFTQPEHLLWSEIGSQPWVYFVHSYYVDPTDSQVT
AATVTHGHQTVTAAVGKDNLIAVQFHPEKSSTAGLKILSNFVSKVIPKNLALAS

Sequences:

>Translated_214_residues
MSTIAVIDYDMGNLHSVCKGLEKAGATPKITDSSIEIDKADAVILPGVGSFDPAVQHLRTRNLEIPIKQVIASGKPFLGI
CLGLQILFESSEEGQEPGLGIFAGKVCRFKSEPGLTIPQMGWNKLQFTQPEHLLWSEIGSQPWVYFVHSYYVDPTDSQVT
AATVTHGHQTVTAAVGKDNLIAVQFHPEKSSTAGLKILSNFVSKVIPKNLALAS
>Mature_213_residues
STIAVIDYDMGNLHSVCKGLEKAGATPKITDSSIEIDKADAVILPGVGSFDPAVQHLRTRNLEIPIKQVIASGKPFLGIC
LGLQILFESSEEGQEPGLGIFAGKVCRFKSEPGLTIPQMGWNKLQFTQPEHLLWSEIGSQPWVYFVHSYYVDPTDSQVTA
ATVTHGHQTVTAAVGKDNLIAVQFHPEKSSTAGLKILSNFVSKVIPKNLALAS

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=202, Percent_Identity=38.6138613861386, Blast_Score=120, Evalue=8e-29,
Organism=Saccharomyces cerevisiae, GI6319725, Length=218, Percent_Identity=34.8623853211009, Blast_Score=122, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS5_TRIEI (Q113E7)

Other databases:

- EMBL:   CP000393
- RefSeq:   YP_721850.1
- ProteinModelPortal:   Q113E7
- SMR:   Q113E7
- STRING:   Q113E7
- GeneID:   4244116
- GenomeReviews:   CP000393_GR
- KEGG:   ter:Tery_2143
- NMPDR:   fig|203124.1.peg.6241
- eggNOG:   COG0118
- HOGENOM:   HBG292341
- OMA:   RPFFGIC
- PhylomeDB:   Q113E7
- ProtClustDB:   PRK13141
- BioCyc:   TERY203124:TERY_2143-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.-

Molecular weight: Translated: 23098; Mature: 22967

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 81-81 ACT_SITE 186-186 ACT_SITE 188-188

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTIAVIDYDMGNLHSVCKGLEKAGATPKITDSSIEIDKADAVILPGVGSFDPAVQHLRT
CCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCEEECCCCEEEECCCCCCCHHHHHHHH
RNLEIPIKQVIASGKPFLGICLGLQILFESSEEGQEPGLGIFAGKVCRFKSEPGLTIPQM
CCCCCCHHHHHHCCCCHHHHHHHHHHEECCCCCCCCCCCEEEECCEEEECCCCCCCCCCC
GWNKLQFTQPEHLLWSEIGSQPWVYFVHSYYVDPTDSQVTAATVTHGHQTVTAAVGKDNL
CCCCEECCCCHHHHHHHHCCCCEEEEEEEEEECCCCCCEEEEEEECCCEEEEEEECCCCE
IAVQFHPEKSSTAGLKILSNFVSKVIPKNLALAS
EEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
STIAVIDYDMGNLHSVCKGLEKAGATPKITDSSIEIDKADAVILPGVGSFDPAVQHLRT
CEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCEEECCCCEEEECCCCCCCHHHHHHHH
RNLEIPIKQVIASGKPFLGICLGLQILFESSEEGQEPGLGIFAGKVCRFKSEPGLTIPQM
CCCCCCHHHHHHCCCCHHHHHHHHHHEECCCCCCCCCCCEEEECCEEEECCCCCCCCCCC
GWNKLQFTQPEHLLWSEIGSQPWVYFVHSYYVDPTDSQVTAATVTHGHQTVTAAVGKDNL
CCCCEECCCCHHHHHHHHCCCCEEEEEEEEEECCCCCCEEEEEEECCCEEEEEEECCCCE
IAVQFHPEKSSTAGLKILSNFVSKVIPKNLALAS
EEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA