Click here to switch to the map view.

The map label for this gene is trmD

Identifier: 113475626

GI number: 113475626

Start: 3040869

End: 3041552

Strand: Reverse

Name: trmD

Synonym: Tery_1962

Alternate gene names: 113475626

Gene position: 3041552-3040869 (Counterclockwise)

Preceding gene: 113475627

Following gene: 113475625

Centisome position: 39.25

GC content: 36.84

Gene sequence:

>684_bases
GTGCGATTTGATGTTATTACCCTGTTCCCAGATTTTTTTACAACACCTCTAAATTCAGGTTTACTAGGGAGAGCCTTTAA
CAAAAATATTGCCAAGGTTAATCTAGTTAATCCTCGTAATTATACCACAGATAAGTATAAAAAAGTTGATGATGAGTCTT
ATGGTGGAGGAGTAGGAATGGTTTTAAAACCAGAGCCTATATTTGCTGCTGTAGAATCATTGCCAACTCTTTCCAAACGA
GAAGTAATTCTATTAACTCCTCAGGGAAAAAGAATGCACCAAGGACTATTTCGAGAACTAGCTACAGATTATGAACAACT
AATTCTAATTTGTGGCCATTATGAAGGTATTGATGAAAGAGTCCAATATTTAGTTAGTAGAGAAGTATCTTTAGGAGATT
TTGTCCTTACAGGCGGAGAAATACCAGCTTTAGCTTTAATTAATGGTGTAGTTCGACTACTTCCTGGAACTGTAGGAAAG
GCTGAATCCTTAGAATGTGAAAGTTTTGAATCGGGATTACTTGACTATCCTCAATATACTCGACCTGCTAACTTTCGTGG
TTGGAAAGTTCCTGAAGTTTTACTCTCAGGGCATCATGCCGAAATAGCAAGATGGCGTTATCAACAACAACTTCAGAGAA
CAAAATCTCGTCGTCCAGATTTGCTCAAAAATGATGATAATTGA

Upstream 100 bases:

>100_bases
TTATGGCAATTACATTAATTACATACCTTTTGCGCAATTATTGTAACTCTCAAGAATGTCATACATTTTTTATAAAACCT
TATAACTTAATTTTACTTCT

Downstream 100 bases:

>100_bases
TTGAATCAAAATTTTGTGAAAATATTTTAAGGCTTAAAGGCAGGAAATAGGAAATAGGCAATAGCCCATAATTTTCATGT
AAAATTTTTTGCATTTTTCC

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 227; Mature: 227

Protein sequence:

>227_residues
MRFDVITLFPDFFTTPLNSGLLGRAFNKNIAKVNLVNPRNYTTDKYKKVDDESYGGGVGMVLKPEPIFAAVESLPTLSKR
EVILLTPQGKRMHQGLFRELATDYEQLILICGHYEGIDERVQYLVSREVSLGDFVLTGGEIPALALINGVVRLLPGTVGK
AESLECESFESGLLDYPQYTRPANFRGWKVPEVLLSGHHAEIARWRYQQQLQRTKSRRPDLLKNDDN

Sequences:

>Translated_227_residues
MRFDVITLFPDFFTTPLNSGLLGRAFNKNIAKVNLVNPRNYTTDKYKKVDDESYGGGVGMVLKPEPIFAAVESLPTLSKR
EVILLTPQGKRMHQGLFRELATDYEQLILICGHYEGIDERVQYLVSREVSLGDFVLTGGEIPALALINGVVRLLPGTVGK
AESLECESFESGLLDYPQYTRPANFRGWKVPEVLLSGHHAEIARWRYQQQLQRTKSRRPDLLKNDDN
>Mature_227_residues
MRFDVITLFPDFFTTPLNSGLLGRAFNKNIAKVNLVNPRNYTTDKYKKVDDESYGGGVGMVLKPEPIFAAVESLPTLSKR
EVILLTPQGKRMHQGLFRELATDYEQLILICGHYEGIDERVQYLVSREVSLGDFVLTGGEIPALALINGVVRLLPGTVGK
AESLECESFESGLLDYPQYTRPANFRGWKVPEVLLSGHHAEIARWRYQQQLQRTKSRRPDLLKNDDN

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=227, Percent_Identity=45.8149779735683, Blast_Score=202, Evalue=1e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_TRIEI (Q113W0)

Other databases:

- EMBL:   CP000393
- RefSeq:   YP_721687.1
- ProteinModelPortal:   Q113W0
- SMR:   Q113W0
- STRING:   Q113W0
- GeneID:   4244386
- GenomeReviews:   CP000393_GR
- KEGG:   ter:Tery_1962
- NMPDR:   fig|203124.1.peg.800
- eggNOG:   COG0336
- HOGENOM:   HBG285805
- OMA:   NRYDLYL
- PhylomeDB:   Q113W0
- ProtClustDB:   PRK00026
- BioCyc:   TERY203124:TERY_1962-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 25656; Mature: 25656

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: NA

Important sites: BINDING 112-112

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFDVITLFPDFFTTPLNSGLLGRAFNKNIAKVNLVNPRNYTTDKYKKVDDESYGGGVGM
CCCEEEEECCHHHHCCCCCCCCCHHHCCCCEEEEEECCCCCCCHHHHCCCCCCCCCCEEE
VLKPEPIFAAVESLPTLSKREVILLTPQGKRMHQGLFRELATDYEQLILICGHYEGIDER
EECCCHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHCHHHHEEHCCCCCCHHHH
VQYLVSREVSLGDFVLTGGEIPALALINGVVRLLPGTVGKAESLECESFESGLLDYPQYT
HHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCC
RPANFRGWKVPEVLLSGHHAEIARWRYQQQLQRTKSRRPDLLKNDDN
CCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MRFDVITLFPDFFTTPLNSGLLGRAFNKNIAKVNLVNPRNYTTDKYKKVDDESYGGGVGM
CCCEEEEECCHHHHCCCCCCCCCHHHCCCCEEEEEECCCCCCCHHHHCCCCCCCCCCEEE
VLKPEPIFAAVESLPTLSKREVILLTPQGKRMHQGLFRELATDYEQLILICGHYEGIDER
EECCCHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHCHHHHEEHCCCCCCHHHH
VQYLVSREVSLGDFVLTGGEIPALALINGVVRLLPGTVGKAESLECESFESGLLDYPQYT
HHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCC
RPANFRGWKVPEVLLSGHHAEIARWRYQQQLQRTKSRRPDLLKNDDN
CCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA