Click here to switch to the map view.

The map label for this gene is glgX [H]

Identifier: 113475562

GI number: 113475562

Start: 2916260

End: 2918377

Strand: Reverse

Name: glgX [H]

Synonym: Tery_1895

Alternate gene names: 113475562

Gene position: 2918377-2916260 (Counterclockwise)

Preceding gene: 113475568

Following gene: 113475561

Centisome position: 37.66

GC content: 37.25

Gene sequence:

>2118_bases
ATGAATCTTAATATCTTACCCGGTAATACTTTTCCCATTGGTGCCACTGTCTGCCAAGACCCCAAAGGAGTAAACTTCTC
TATCTATTCCAAAAATGCTACAGCAATAGAATTACTACTATTTGATGAACCATTAGCAGCTAAACCAAAGCAAGTAATTT
TACTTGACCCAACACAACACAGAACTCATCATTACTGGCATATTTTTATTTCTGGTATTGGAGCGGGACAAATATATGCC
TATCGAGTCTATGGACCCTTTAACCCAGAAAAAGGTCATCGGTTTGATGGTACAAAAGTTTTACTTGACCCTTATGCTAG
AATTGTAGTGGGACAAGAAAAATATAGTCGTAAAGCTGCTACAGGTAACGGTAATAATTGTGCTTACGCTCTCAAGGGGG
TGGTAGTTGACCCAACTACCTATGACTGGGAAGATGACAAACCTTTAGAAACTCCCTATGCTAAAACAGTTATCTATGAA
ATGCATGTTGGTGGATTTACTCGTCATCCTAATTCTGGTGTTGCTCCTGAAAAACGGGGTACTTTTGCTGGGCTAATAGA
AAAAATTCCCTATCTCAAAGAATTGGGAATTACTGCAGTGGAATTATTACCAGTCCAGCAATTTGATGAACAAGATGTGC
GCGAACCCCTAATCAATTATTGGGGCTATAGTCAGATCGCTTTTTTTGCACCTCACCACAGTTATAGTTCCCGCAAAGAT
ATTTTCGGACCAATGGATGAGTTCCGAGATATGGTAAAGGCATTGCATAAAGAAGGAATAGAAGTCATTCTGGATGTAGT
TTTTAATCACACTGCAGAGGGTAACGAAAACGGACCAACAGTTTCTTTCCGGGGGTTAGAAAACTCAACTTACTACATTC
TAGAAAAGAATGATCGTGGATACTACAGCAATTATACCGGTTGTGGCAATACCTTCAATACAAATAATCCTTTTGTACAT
CGCTTAATTGTTGACTGTCTTTGTTACTGGGTTAGAGAAATGCATGTTGATGGGTTCCGGTTTGACTTGGCATCTGTTAT
GTCTCGGAGCATGACTGGTGATCCCTTAGAAGATCCTCCTGTTTTGTGGGCAATTGAGTCAGATCCGGTTTTGGCAGGCA
CAAAAATTATTGCTGAAGCTTGGGATGCAGCCGGACTTTATCAGGTGGGTTCATTTATTGGCGATCGCTTTGCTGAATGG
AACGGACATTATCGTGACGATGTTCGTCAATTTGTCAAAAGTGATCCCCAAATAGTTGAGAAATTGGCAGCAAGAATTAT
GGGTAGTCCTGATATTTATCCTAAACCAGATCGAGAACCAAATCATAGTATTAACTTTATTACTTGTCATGATGGTTTTA
CTATGAATGATCTGGTTTCTTATAATGAGAAACATAATGAAGCTAATTGTGAAGATAACCGCGATGGTGCTAATCATAAT
TTTAGTTGGAATTGTGGAATTGAAGGAACTACAGATCAAGAAGAAATTGAAATGTTAAGACTGCGACAAATTAAGAACTT
TTTTACTATACTGTTTTTTTCTCAAGGTACACCTATGATTTTGATGGGAGATGAAGTCAGGCGAACTCAATTAGGTAACA
ACAATAGTTATTGTCAGAATAATAAAAGTAGTTGGTTTGACTGGAGTCAAATTGATCAACAAAATGATTTATTACGATTT
GTCAAAAAGATTATTCATTTCACTCAAGAATTACAAATTACACAGTTAGATAAGGTTTTAGCTACTGTAGGAGCGATACC
CGAATCATCTTTGGTTAGCACTAAAGAGAAAACTAACTTGAATAAAGTACCTAATATTAGTTGGCATGGAATACATTTAG
GTATACCTGATTGGAATGTAGGTTCCAGGACTCTAGCTTTTAGTTTAAAACATCCAGAAAGTGGTGAATATTTTCACATT
ATGTTGAATGCTTATTGGAAACCTTTAATGTTTGAATTGCCACCTTTGGAAAGGGGGGAAAATTGGTATTTAATTGTTAA
TACTGCTTTTACTTCTCCTAAAGATTTTAATAACCTTGATGTGGCAACAAAAGTTATTGGTGTTAGATACCGCTTAGAGC
CCCGGGCTGCTGTTATTTTAATGACAAAAAGAGATTAG

Upstream 100 bases:

>100_bases
TTTTCCCGAAACCTGATTTACTGTGGGTAAATCTTAAAATATCCACTTATATCTTATAAAATTACTGAATTTATCTAACA
ATTTGCGTAACTCAAAAATC

Downstream 100 bases:

>100_bases
GCAGTCCTGAAGTTAGAACTCAGAAGTCAGAAGTTATATAATAGAGTTGTTGGGAATTCAGAAAAAAATGACTATAACCA
TTATCATTCAAAACTTATAG

Product: glycogen debranching enzyme GlgX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 705; Mature: 705

Protein sequence:

>705_residues
MNLNILPGNTFPIGATVCQDPKGVNFSIYSKNATAIELLLFDEPLAAKPKQVILLDPTQHRTHHYWHIFISGIGAGQIYA
YRVYGPFNPEKGHRFDGTKVLLDPYARIVVGQEKYSRKAATGNGNNCAYALKGVVVDPTTYDWEDDKPLETPYAKTVIYE
MHVGGFTRHPNSGVAPEKRGTFAGLIEKIPYLKELGITAVELLPVQQFDEQDVREPLINYWGYSQIAFFAPHHSYSSRKD
IFGPMDEFRDMVKALHKEGIEVILDVVFNHTAEGNENGPTVSFRGLENSTYYILEKNDRGYYSNYTGCGNTFNTNNPFVH
RLIVDCLCYWVREMHVDGFRFDLASVMSRSMTGDPLEDPPVLWAIESDPVLAGTKIIAEAWDAAGLYQVGSFIGDRFAEW
NGHYRDDVRQFVKSDPQIVEKLAARIMGSPDIYPKPDREPNHSINFITCHDGFTMNDLVSYNEKHNEANCEDNRDGANHN
FSWNCGIEGTTDQEEIEMLRLRQIKNFFTILFFSQGTPMILMGDEVRRTQLGNNNSYCQNNKSSWFDWSQIDQQNDLLRF
VKKIIHFTQELQITQLDKVLATVGAIPESSLVSTKEKTNLNKVPNISWHGIHLGIPDWNVGSRTLAFSLKHPESGEYFHI
MLNAYWKPLMFELPPLERGENWYLIVNTAFTSPKDFNNLDVATKVIGVRYRLEPRAAVILMTKRD

Sequences:

>Translated_705_residues
MNLNILPGNTFPIGATVCQDPKGVNFSIYSKNATAIELLLFDEPLAAKPKQVILLDPTQHRTHHYWHIFISGIGAGQIYA
YRVYGPFNPEKGHRFDGTKVLLDPYARIVVGQEKYSRKAATGNGNNCAYALKGVVVDPTTYDWEDDKPLETPYAKTVIYE
MHVGGFTRHPNSGVAPEKRGTFAGLIEKIPYLKELGITAVELLPVQQFDEQDVREPLINYWGYSQIAFFAPHHSYSSRKD
IFGPMDEFRDMVKALHKEGIEVILDVVFNHTAEGNENGPTVSFRGLENSTYYILEKNDRGYYSNYTGCGNTFNTNNPFVH
RLIVDCLCYWVREMHVDGFRFDLASVMSRSMTGDPLEDPPVLWAIESDPVLAGTKIIAEAWDAAGLYQVGSFIGDRFAEW
NGHYRDDVRQFVKSDPQIVEKLAARIMGSPDIYPKPDREPNHSINFITCHDGFTMNDLVSYNEKHNEANCEDNRDGANHN
FSWNCGIEGTTDQEEIEMLRLRQIKNFFTILFFSQGTPMILMGDEVRRTQLGNNNSYCQNNKSSWFDWSQIDQQNDLLRF
VKKIIHFTQELQITQLDKVLATVGAIPESSLVSTKEKTNLNKVPNISWHGIHLGIPDWNVGSRTLAFSLKHPESGEYFHI
MLNAYWKPLMFELPPLERGENWYLIVNTAFTSPKDFNNLDVATKVIGVRYRLEPRAAVILMTKRD
>Mature_705_residues
MNLNILPGNTFPIGATVCQDPKGVNFSIYSKNATAIELLLFDEPLAAKPKQVILLDPTQHRTHHYWHIFISGIGAGQIYA
YRVYGPFNPEKGHRFDGTKVLLDPYARIVVGQEKYSRKAATGNGNNCAYALKGVVVDPTTYDWEDDKPLETPYAKTVIYE
MHVGGFTRHPNSGVAPEKRGTFAGLIEKIPYLKELGITAVELLPVQQFDEQDVREPLINYWGYSQIAFFAPHHSYSSRKD
IFGPMDEFRDMVKALHKEGIEVILDVVFNHTAEGNENGPTVSFRGLENSTYYILEKNDRGYYSNYTGCGNTFNTNNPFVH
RLIVDCLCYWVREMHVDGFRFDLASVMSRSMTGDPLEDPPVLWAIESDPVLAGTKIIAEAWDAAGLYQVGSFIGDRFAEW
NGHYRDDVRQFVKSDPQIVEKLAARIMGSPDIYPKPDREPNHSINFITCHDGFTMNDLVSYNEKHNEANCEDNRDGANHN
FSWNCGIEGTTDQEEIEMLRLRQIKNFFTILFFSQGTPMILMGDEVRRTQLGNNNSYCQNNKSSWFDWSQIDQQNDLLRF
VKKIIHFTQELQITQLDKVLATVGAIPESSLVSTKEKTNLNKVPNISWHGIHLGIPDWNVGSRTLAFSLKHPESGEYFHI
MLNAYWKPLMFELPPLERGENWYLIVNTAFTSPKDFNNLDVATKVIGVRYRLEPRAAVILMTKRD

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=575, Percent_Identity=45.0434782608696, Blast_Score=441, Evalue=1e-125,
Organism=Escherichia coli, GI1789839, Length=351, Percent_Identity=24.2165242165242, Blast_Score=79, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 80301; Mature: 80301

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLNILPGNTFPIGATVCQDPKGVNFSIYSKNATAIELLLFDEPLAAKPKQVILLDPTQH
CCCEEECCCCCCCCCEEECCCCCCEEEEEECCCCEEEEEEECCCCCCCCCEEEEECCCCC
RTHHYWHIFISGIGAGQIYAYRVYGPFNPEKGHRFDGTKVLLDPYARIVVGQEKYSRKAA
CCCEEEEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCEEEECCCEEEEECCHHHCCCCC
TGNGNNCAYALKGVVVDPTTYDWEDDKPLETPYAKTVIYEMHVGGFTRHPNSGVAPEKRG
CCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEEEECCCEEECCCCCCCCCCCC
TFAGLIEKIPYLKELGITAVELLPVQQFDEQDVREPLINYWGYSQIAFFAPHHSYSSRKD
HHHHHHHHCCHHHHCCCEEEEEEECHHCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCC
IFGPMDEFRDMVKALHKEGIEVILDVVFNHTAEGNENGPTVSFRGLENSTYYILEKNDRG
CCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCEEEEEECCCCEEEEEECCCCC
YYSNYTGCGNTFNTNNPFVHRLIVDCLCYWVREMHVDGFRFDLASVMSRSMTGDPLEDPP
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCC
VLWAIESDPVLAGTKIIAEAWDAAGLYQVGSFIGDRFAEWNGHYRDDVRQFVKSDPQIVE
EEEEECCCCCEECHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHH
KLAARIMGSPDIYPKPDREPNHSINFITCHDGFTMNDLVSYNEKHNEANCEDNRDGANHN
HHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHCCCCCCCCCCCCCCCCCCC
FSWNCGIEGTTDQEEIEMLRLRQIKNFFTILFFSQGTPMILMGDEVRRTQLGNNNSYCQN
CEEECCCCCCCCHHHHHHHHHHHHHHEEEEEEECCCCCEEEECCHHHHHHCCCCCCCCCC
NKSSWFDWSQIDQQNDLLRFVKKIIHFTQELQITQLDKVLATVGAIPESSLVSTKEKTNL
CCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHCCC
NKVPNISWHGIHLGIPDWNVGSRTLAFSLKHPESGEYFHIMLNAYWKPLMFELPPLERGE
CCCCCCEEEEEEECCCCCCCCCEEEEEEECCCCCCCEEEEEEECCCCCCEEECCCCCCCC
NWYLIVNTAFTSPKDFNNLDVATKVIGVRYRLEPRAAVILMTKRD
CEEEEEEECCCCCCCCCCCHHHHHEEEEEEEECCCEEEEEEEECC
>Mature Secondary Structure
MNLNILPGNTFPIGATVCQDPKGVNFSIYSKNATAIELLLFDEPLAAKPKQVILLDPTQH
CCCEEECCCCCCCCCEEECCCCCCEEEEEECCCCEEEEEEECCCCCCCCCEEEEECCCCC
RTHHYWHIFISGIGAGQIYAYRVYGPFNPEKGHRFDGTKVLLDPYARIVVGQEKYSRKAA
CCCEEEEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCEEEECCCEEEEECCHHHCCCCC
TGNGNNCAYALKGVVVDPTTYDWEDDKPLETPYAKTVIYEMHVGGFTRHPNSGVAPEKRG
CCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEEEECCCEEECCCCCCCCCCCC
TFAGLIEKIPYLKELGITAVELLPVQQFDEQDVREPLINYWGYSQIAFFAPHHSYSSRKD
HHHHHHHHCCHHHHCCCEEEEEEECHHCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCC
IFGPMDEFRDMVKALHKEGIEVILDVVFNHTAEGNENGPTVSFRGLENSTYYILEKNDRG
CCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCEEEEEECCCCEEEEEECCCCC
YYSNYTGCGNTFNTNNPFVHRLIVDCLCYWVREMHVDGFRFDLASVMSRSMTGDPLEDPP
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCC
VLWAIESDPVLAGTKIIAEAWDAAGLYQVGSFIGDRFAEWNGHYRDDVRQFVKSDPQIVE
EEEEECCCCCEECHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHH
KLAARIMGSPDIYPKPDREPNHSINFITCHDGFTMNDLVSYNEKHNEANCEDNRDGANHN
HHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHCCCCCCCCCCCCCCCCCCC
FSWNCGIEGTTDQEEIEMLRLRQIKNFFTILFFSQGTPMILMGDEVRRTQLGNNNSYCQN
CEEECCCCCCCCHHHHHHHHHHHHHHEEEEEEECCCCCEEEECCHHHHHHCCCCCCCCCC
NKSSWFDWSQIDQQNDLLRFVKKIIHFTQELQITQLDKVLATVGAIPESSLVSTKEKTNL
CCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHCCC
NKVPNISWHGIHLGIPDWNVGSRTLAFSLKHPESGEYFHIMLNAYWKPLMFELPPLERGE
CCCCCCEEEEEEECCCCCCCCCEEEEEEECCCCCCCEEEEEEECCCCCCEEECCCCCCCC
NWYLIVNTAFTSPKDFNNLDVATKVIGVRYRLEPRAAVILMTKRD
CEEEEEEECCCCCCCCCCCHHHHHEEEEEEEECCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]