Click here to switch to the map view.

The map label for this gene is epsE [H]

Identifier: 113475420

GI number: 113475420

Start: 2661209

End: 2663380

Strand: Reverse

Name: epsE [H]

Synonym: Tery_1748

Alternate gene names: 113475420

Gene position: 2663380-2661209 (Counterclockwise)

Preceding gene: 113475427

Following gene: 113475419

Centisome position: 34.37

GC content: 38.81

Gene sequence:

>2172_bases
ATGGCCCAAAGCTCTTCACGCAATCCACGCACCGCTATAGTTGCCCTCGGTAATCTGTCCCCTTTTGGGAATAAACTGGT
TCAGGCAGGCTTAGTTAACATTGAACAATTCCAACATTGCCAAATTGAAAGCCGTAAGACAGGCAAATCACTGACAGATT
TGGTCGAAGATGCAATAGGACATCCCTTACCGCCAGATTTAATTAGGCATTATAAAAAGCAGCAGCTATTTGAATTGATG
ATTCTACATGGTGTAGCTACTTTTGACCCAGAAATTACTCCAATACCACTAGAGCAGTTATGTTATTTAATCGACAAGAT
AATTCCTATTGAAACTTGTCGTCGGAATCAGATTATACCACTCTTTAGTCGCGAAACTGATTTAGCAAATCAGTTCGTCC
AAATAGGAAAGATAAGTCAAGAAAAAAGTTTAAAGGCACTGAGGCAAAGCAGTGATTCTGAAAGTAGTTTAGTTGATGAA
TTAGAAAAGTTTGCAGGAGATTCATTACCATCAAGTTTAATGAAAGATTATGAAAATCAGCAACCATTTGTAATGATGGG
AATGGTTAATCCTCATAATCTTCAAGCTTTGGATGAGTTAAAAAATATCTTGAGGTATCGTGGATTAACTTTACAACGCT
TGGTTATTACTAAACAAGATTATGAAAGTCTGATTAACTATTACTTAGAGGAACAAAGCAAGAAGAATATTGTTAAGGCA
AAAGAGGCAGAACAAAAAGAGCTAGAGTTAGAGTCTAATTTCTTTGAAAGTGATAATCAGTTAGAGGAAGCTGATGATGA
AAAAGAAGTAGAAGATATTGCTGAAAGTATCTCTGCTGCTGAGGATGCTCCTGTTATTAAAGCAGTAAATATTATTTTAG
TAAAAGCACTGACTGAGGGTGTATCCGATATTCATGTCGAGCCACAAGAAGAGTTTATCAGAATCAGGATGCGCAAAGAT
GGGGTATTACAGGAATATTTCCGATTTCCGAAAAAGGTGATTCCGGCTATTACTTCACGATTTAAAATTATTGCAAGTTT
AGATATTGCAGAACGAAGGCAAGCACAAGATGGTCGTATCCGCAAAGTTTTTAAGGGGCGTACAATTGACTTCCGGGTCA
ATAGTTTGCCTAGTCGCTATGGTGAAAAAATAGTCTTGCGGATTTTGGATAGCTCTAGTACCCAACTGGGGTTAGATAAG
TTAATCACAGATAAAGCCACTTTGGCAATGGTGAGGGAAACTGCTAGTCGTCCATTTGGATTAATTCTGGTGACTGGGCC
AACTGGTTCTGGTAAGTCTACTTCTCTATATTCGATTTTGGCAGAACGAAATGACCCAAGTATTAATATTAGTACGGCAG
AAGACCCAATAGAATATGCTTTGCCGGGAATTACTCAATGTCAGGTAATTAGGGAAAAGGATTTAACTTTTGCGAATATT
CTACGGGCATTTCTGCGACAGGACCCGGATGTTTTACTAGTAGGGGAAACACGAGATAAGGAAACGGCAAAAACGGCAAT
TGAAGCTGCGTTAACAGGGCACTTGGTTTTGACAACTCTACATACTAATGATGCTGCCGGAGCGATCGCTCGTCTTGATG
AAATGGGAGTGGAATCTTTTATGGTTTCAGCGTCTTTGTTGGGTGTAGTAGCACAACGCCTGATGCGTCGGGTTTGCAGT
AAATGTCGAATAGAATATCACCCTGAACCTGATGAGTTTCGTCGTTATGGTTTGGTTCCTTCTCAAGAAAAAGATTTTAC
ATACTATAAAGCAAATACTATACCAATTGACCAACGAGAAGATTTAAAACAAAGGGGAGAGTTATGTCCCAAATGTAATG
GTATTGGTTATAAAGGTCGTGTAGGTGTTTATGAGTTTATGAAAATTACTGAGCGATTACAAACTTTAATTACTCAAAGG
GTACCGACGGAACAAATTAAGGAAGCTGCAGTAGAAGAGGGGATGAAGCCTCTGTTACAATATAGTCTGAATTTGGTACG
AGAAGGTTATACAACTTTTGAGGAGGTGGAACGGGTAACATTTACTGATTCTGGTCTGGAGGCGGAAATGAAACGCTTGG
CTAAACAAAATCTAATATGTAAGTGTTGTAACGCTGAGTTACAAGCAGAATGGTTAGAATGTCCTTACTGTATGACTCCT
CGCTTTGATTAG

Upstream 100 bases:

>100_bases
AATATTGAATCAGTAAATTCGGTTAACAGCTTATTAAGCAAGTATAAATTTTATCAAAAACTTAGGGAATATTTAGATTA
AGGAAAAAAATTTATTCATC

Downstream 100 bases:

>100_bases
GAGTAGAAAGTAAAGGGTTGGAGGTTGGGCAAAAGGGGGTGGAAAAGGTTTGTTATCAATAAATACTTAAGACGAACAGT
TAAAAATTATTTTCCCAGGA

Product: type II secretion system protein E

Products: NA

Alternate protein names: Cholera toxin secretion protein epsE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 723; Mature: 722

Protein sequence:

>723_residues
MAQSSSRNPRTAIVALGNLSPFGNKLVQAGLVNIEQFQHCQIESRKTGKSLTDLVEDAIGHPLPPDLIRHYKKQQLFELM
ILHGVATFDPEITPIPLEQLCYLIDKIIPIETCRRNQIIPLFSRETDLANQFVQIGKISQEKSLKALRQSSDSESSLVDE
LEKFAGDSLPSSLMKDYENQQPFVMMGMVNPHNLQALDELKNILRYRGLTLQRLVITKQDYESLINYYLEEQSKKNIVKA
KEAEQKELELESNFFESDNQLEEADDEKEVEDIAESISAAEDAPVIKAVNIILVKALTEGVSDIHVEPQEEFIRIRMRKD
GVLQEYFRFPKKVIPAITSRFKIIASLDIAERRQAQDGRIRKVFKGRTIDFRVNSLPSRYGEKIVLRILDSSSTQLGLDK
LITDKATLAMVRETASRPFGLILVTGPTGSGKSTSLYSILAERNDPSINISTAEDPIEYALPGITQCQVIREKDLTFANI
LRAFLRQDPDVLLVGETRDKETAKTAIEAALTGHLVLTTLHTNDAAGAIARLDEMGVESFMVSASLLGVVAQRLMRRVCS
KCRIEYHPEPDEFRRYGLVPSQEKDFTYYKANTIPIDQREDLKQRGELCPKCNGIGYKGRVGVYEFMKITERLQTLITQR
VPTEQIKEAAVEEGMKPLLQYSLNLVREGYTTFEEVERVTFTDSGLEAEMKRLAKQNLICKCCNAELQAEWLECPYCMTP
RFD

Sequences:

>Translated_723_residues
MAQSSSRNPRTAIVALGNLSPFGNKLVQAGLVNIEQFQHCQIESRKTGKSLTDLVEDAIGHPLPPDLIRHYKKQQLFELM
ILHGVATFDPEITPIPLEQLCYLIDKIIPIETCRRNQIIPLFSRETDLANQFVQIGKISQEKSLKALRQSSDSESSLVDE
LEKFAGDSLPSSLMKDYENQQPFVMMGMVNPHNLQALDELKNILRYRGLTLQRLVITKQDYESLINYYLEEQSKKNIVKA
KEAEQKELELESNFFESDNQLEEADDEKEVEDIAESISAAEDAPVIKAVNIILVKALTEGVSDIHVEPQEEFIRIRMRKD
GVLQEYFRFPKKVIPAITSRFKIIASLDIAERRQAQDGRIRKVFKGRTIDFRVNSLPSRYGEKIVLRILDSSSTQLGLDK
LITDKATLAMVRETASRPFGLILVTGPTGSGKSTSLYSILAERNDPSINISTAEDPIEYALPGITQCQVIREKDLTFANI
LRAFLRQDPDVLLVGETRDKETAKTAIEAALTGHLVLTTLHTNDAAGAIARLDEMGVESFMVSASLLGVVAQRLMRRVCS
KCRIEYHPEPDEFRRYGLVPSQEKDFTYYKANTIPIDQREDLKQRGELCPKCNGIGYKGRVGVYEFMKITERLQTLITQR
VPTEQIKEAAVEEGMKPLLQYSLNLVREGYTTFEEVERVTFTDSGLEAEMKRLAKQNLICKCCNAELQAEWLECPYCMTP
RFD
>Mature_722_residues
AQSSSRNPRTAIVALGNLSPFGNKLVQAGLVNIEQFQHCQIESRKTGKSLTDLVEDAIGHPLPPDLIRHYKKQQLFELMI
LHGVATFDPEITPIPLEQLCYLIDKIIPIETCRRNQIIPLFSRETDLANQFVQIGKISQEKSLKALRQSSDSESSLVDEL
EKFAGDSLPSSLMKDYENQQPFVMMGMVNPHNLQALDELKNILRYRGLTLQRLVITKQDYESLINYYLEEQSKKNIVKAK
EAEQKELELESNFFESDNQLEEADDEKEVEDIAESISAAEDAPVIKAVNIILVKALTEGVSDIHVEPQEEFIRIRMRKDG
VLQEYFRFPKKVIPAITSRFKIIASLDIAERRQAQDGRIRKVFKGRTIDFRVNSLPSRYGEKIVLRILDSSSTQLGLDKL
ITDKATLAMVRETASRPFGLILVTGPTGSGKSTSLYSILAERNDPSINISTAEDPIEYALPGITQCQVIREKDLTFANIL
RAFLRQDPDVLLVGETRDKETAKTAIEAALTGHLVLTTLHTNDAAGAIARLDEMGVESFMVSASLLGVVAQRLMRRVCSK
CRIEYHPEPDEFRRYGLVPSQEKDFTYYKANTIPIDQREDLKQRGELCPKCNGIGYKGRVGVYEFMKITERLQTLITQRV
PTEQIKEAAVEEGMKPLLQYSLNLVREGYTTFEEVERVTFTDSGLEAEMKRLAKQNLICKCCNAELQAEWLECPYCMTPR
FD

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=465, Percent_Identity=43.4408602150538, Blast_Score=350, Evalue=2e-97,
Organism=Escherichia coli, GI1786296, Length=393, Percent_Identity=39.6946564885496, Blast_Score=278, Evalue=1e-75,
Organism=Escherichia coli, GI87082188, Length=200, Percent_Identity=40.5, Blast_Score=114, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 81884; Mature: 81753

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQSSSRNPRTAIVALGNLSPFGNKLVQAGLVNIEQFQHCQIESRKTGKSLTDLVEDAIG
CCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHC
HPLPPDLIRHYKKQQLFELMILHGVATFDPEITPIPLEQLCYLIDKIIPIETCRRNQIIP
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHCCCCCCE
LFSRETDLANQFVQIGKISQEKSLKALRQSSDSESSLVDELEKFAGDSLPSSLMKDYENQ
EECCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCC
QPFVMMGMVNPHNLQALDELKNILRYRGLTLQRLVITKQDYESLINYYLEEQSKKNIVKA
CCEEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KEAEQKELELESNFFESDNQLEEADDEKEVEDIAESISAAEDAPVIKAVNIILVKALTEG
HHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCC
VSDIHVEPQEEFIRIRMRKDGVLQEYFRFPKKVIPAITSRFKIIASLDIAERRQAQDGRI
HHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCHH
RKVFKGRTIDFRVNSLPSRYGEKIVLRILDSSSTQLGLDKLITDKATLAMVRETASRPFG
HHHHCCCEEEEEECCCCHHHHHEEEHHEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEE
LILVTGPTGSGKSTSLYSILAERNDPSINISTAEDPIEYALPGITQCQVIREKDLTFANI
EEEEECCCCCCCCHHHHHHHHCCCCCCEEEECCCCCHHHHCCCCHHHHHHHHCCCCHHHH
LRAFLRQDPDVLLVGETRDKETAKTAIEAALTGHLVLTTLHTNDAAGAIARLDEMGVESF
HHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHHHHH
MVSASLLGVVAQRLMRRVCSKCRIEYHPEPDEFRRYGLVPSQEKDFTYYKANTIPIDQRE
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCEEEECCCCCCCCHH
DLKQRGELCPKCNGIGYKGRVGVYEFMKITERLQTLITQRVPTEQIKEAAVEEGMKPLLQ
HHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
YSLNLVREGYTTFEEVERVTFTDSGLEAEMKRLAKQNLICKCCNAELQAEWLECPYCMTP
HHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCC
RFD
CCC
>Mature Secondary Structure 
AQSSSRNPRTAIVALGNLSPFGNKLVQAGLVNIEQFQHCQIESRKTGKSLTDLVEDAIG
CCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHC
HPLPPDLIRHYKKQQLFELMILHGVATFDPEITPIPLEQLCYLIDKIIPIETCRRNQIIP
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHCCCCCCE
LFSRETDLANQFVQIGKISQEKSLKALRQSSDSESSLVDELEKFAGDSLPSSLMKDYENQ
EECCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCC
QPFVMMGMVNPHNLQALDELKNILRYRGLTLQRLVITKQDYESLINYYLEEQSKKNIVKA
CCEEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KEAEQKELELESNFFESDNQLEEADDEKEVEDIAESISAAEDAPVIKAVNIILVKALTEG
HHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCC
VSDIHVEPQEEFIRIRMRKDGVLQEYFRFPKKVIPAITSRFKIIASLDIAERRQAQDGRI
HHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCHH
RKVFKGRTIDFRVNSLPSRYGEKIVLRILDSSSTQLGLDKLITDKATLAMVRETASRPFG
HHHHCCCEEEEEECCCCHHHHHEEEHHEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEE
LILVTGPTGSGKSTSLYSILAERNDPSINISTAEDPIEYALPGITQCQVIREKDLTFANI
EEEEECCCCCCCCHHHHHHHHCCCCCCEEEECCCCCHHHHCCCCHHHHHHHHCCCCHHHH
LRAFLRQDPDVLLVGETRDKETAKTAIEAALTGHLVLTTLHTNDAAGAIARLDEMGVESF
HHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHHHHH
MVSASLLGVVAQRLMRRVCSKCRIEYHPEPDEFRRYGLVPSQEKDFTYYKANTIPIDQRE
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCEEEECCCCCCCCHH
DLKQRGELCPKCNGIGYKGRVGVYEFMKITERLQTLITQRVPTEQIKEAAVEEGMKPLLQ
HHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
YSLNLVREGYTTFEEVERVTFTDSGLEAEMKRLAKQNLICKCCNAELQAEWLECPYCMTP
HHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCC
RFD
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423007; 10952301 [H]