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The map label for this gene is pilT [H]

Identifier: 113475419

GI number: 113475419

Start: 2659750

End: 2660862

Strand: Reverse

Name: pilT [H]

Synonym: Tery_1747

Alternate gene names: 113475419

Gene position: 2660862-2659750 (Counterclockwise)

Preceding gene: 113475420

Following gene: 113475418

Centisome position: 34.33

GC content: 42.14

Gene sequence:

>1113_bases
ATGTCTTTGATGATTGAGGATGTTTTAGAATCTTTAGTTGAACAAGGTGGTTCTGATGTTCATATTCAAGCAAGAGCACC
AATTTTTTTCCGTATTAACGGTCAGTTGACTGCTCAAACTCAGTTTGGGGAAAATATGGAACCAATGGTAGTACAAGCTC
TGATTTTCCAAATGCTCAATAATATGCAACGGAAACATTTAGAACAAAACTGGGAGCTTGATAGCGCTTATGGTGTTAAG
GGTTTGGCTCGTTTCCGTCTAAATGTGTATCGGGAGCGGGGTAGTTGGGCTGCTTGTATGCGTGCTTTGGCTTCTAAAAT
TCCTAATGCAGATAAATTAGGGATTCCCATGATTTTACGGGAAATGACGGAGCGACCCAGGGGATTGTTTTTGGTGACGG
GGCAAACAGGTTCTGGGAAGACAACTACGATGGCAGCTTTAATAGATTTGATTAACCGTACTCGTACTGAACATATTTTG
ACTGTGGAAGACCCCATAGAATATGTGTTTCCCAATCAAAAAAGTTTGTTTCATCAACGACAAAAAGGTGAAGATACGAA
AAGTTTTGCTAATGCTCTGAAGGGAGCGTTACGTCAAGACCCTGATATTATTCTGGTTGGGGAAATGCGGGATTTGGAAA
CTATTGGTTTGGCTTGTAGTGCGGCAGAAACAGGTCACTTGGTTTTTGGGACTCTCCACACTAACTCAGCTGCGGGTACG
GTAGACCGGATGTTGGATGTTTTCCCGCCGGAACAACAACCACAAATGAGGTCGCAGTTGGCTAACTCTATTGTTTGTAT
TTGTAGTCAAAACTTAGTTAAGAAAACAGGTGGGGGTCGTTGTGCGGCTCATGAGATTATGTTGAATACTCCGGCGATCG
CTAACTTAATTCGGGAGTCGAAAAATTCTCAGCTTTATTCTCAAATTCAAATGGGCGCTAAGTTAGGAATGCAAACTATG
GAAATGTCTTTGGCTAAACTTTATGAAAAAGGGAATGTGACTTGGGCAAATGCTATGGCTAAGGCAGTTAAGCCTGATGA
ATTAGAGGCTTTGATTGGCCCTGAGCCCAGGGAAACTAAGGCTAAAACTAAGGCTAAAGCTAGAGCTCATTAA

Upstream 100 bases:

>100_bases
GTTTAACTATTTCCACAAGTAAATAGTCAGAATAATTTGGTATAACTGATAAGTCAAAATAATTAGTAACTAATAACTAA
TAAAAGGGAGATTAAATTAT

Downstream 100 bases:

>100_bases
TATTGCAGAAAGAAGAATGAAGGAAAAGGGGGGAAGGAAGATTTTATAAGACTAAGGGCAAGCCAGAAGTTGCGAAGGCT
GGGTTTGAGAATTCACAAAT

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 370; Mature: 369

Protein sequence:

>370_residues
MSLMIEDVLESLVEQGGSDVHIQARAPIFFRINGQLTAQTQFGENMEPMVVQALIFQMLNNMQRKHLEQNWELDSAYGVK
GLARFRLNVYRERGSWAACMRALASKIPNADKLGIPMILREMTERPRGLFLVTGQTGSGKTTTMAALIDLINRTRTEHIL
TVEDPIEYVFPNQKSLFHQRQKGEDTKSFANALKGALRQDPDIILVGEMRDLETIGLACSAAETGHLVFGTLHTNSAAGT
VDRMLDVFPPEQQPQMRSQLANSIVCICSQNLVKKTGGGRCAAHEIMLNTPAIANLIRESKNSQLYSQIQMGAKLGMQTM
EMSLAKLYEKGNVTWANAMAKAVKPDELEALIGPEPRETKAKTKAKARAH

Sequences:

>Translated_370_residues
MSLMIEDVLESLVEQGGSDVHIQARAPIFFRINGQLTAQTQFGENMEPMVVQALIFQMLNNMQRKHLEQNWELDSAYGVK
GLARFRLNVYRERGSWAACMRALASKIPNADKLGIPMILREMTERPRGLFLVTGQTGSGKTTTMAALIDLINRTRTEHIL
TVEDPIEYVFPNQKSLFHQRQKGEDTKSFANALKGALRQDPDIILVGEMRDLETIGLACSAAETGHLVFGTLHTNSAAGT
VDRMLDVFPPEQQPQMRSQLANSIVCICSQNLVKKTGGGRCAAHEIMLNTPAIANLIRESKNSQLYSQIQMGAKLGMQTM
EMSLAKLYEKGNVTWANAMAKAVKPDELEALIGPEPRETKAKTKAKARAH
>Mature_369_residues
SLMIEDVLESLVEQGGSDVHIQARAPIFFRINGQLTAQTQFGENMEPMVVQALIFQMLNNMQRKHLEQNWELDSAYGVKG
LARFRLNVYRERGSWAACMRALASKIPNADKLGIPMILREMTERPRGLFLVTGQTGSGKTTTMAALIDLINRTRTEHILT
VEDPIEYVFPNQKSLFHQRQKGEDTKSFANALKGALRQDPDIILVGEMRDLETIGLACSAAETGHLVFGTLHTNSAAGTV
DRMLDVFPPEQQPQMRSQLANSIVCICSQNLVKKTGGGRCAAHEIMLNTPAIANLIRESKNSQLYSQIQMGAKLGMQTME
MSLAKLYEKGNVTWANAMAKAVKPDELEALIGPEPRETKAKTKAKARAH

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=319, Percent_Identity=41.3793103448276, Blast_Score=248, Evalue=6e-67,
Organism=Escherichia coli, GI1789723, Length=399, Percent_Identity=29.5739348370927, Blast_Score=128, Evalue=6e-31,
Organism=Escherichia coli, GI1786296, Length=284, Percent_Identity=32.0422535211268, Blast_Score=112, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 41023; Mature: 40892

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLMIEDVLESLVEQGGSDVHIQARAPIFFRINGQLTAQTQFGENMEPMVVQALIFQMLN
CCCHHHHHHHHHHHCCCCEEEEEEECCEEEEECCEEEEEECCCCCCCHHHHHHHHHHHHH
NMQRKHLEQNWELDSAYGVKGLARFRLNVYRERGSWAACMRALASKIPNADKLGIPMILR
HHHHHHHHHCCCCCCHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHH
EMTERPRGLFLVTGQTGSGKTTTMAALIDLINRTRTEHILTVEDPIEYVFPNQKSLFHQR
HHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCHHHHCCCHHHHHHHH
QKGEDTKSFANALKGALRQDPDIILVGEMRDLETIGLACSAAETGHLVFGTLHTNSAAGT
HCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHEECCCCCCCEEEEEEECCCCHHH
VDRMLDVFPPEQQPQMRSQLANSIVCICSQNLVKKTGGGRCAAHEIMLNTPAIANLIRES
HHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHCCCCCEEHHHEEECCHHHHHHHHHC
KNSQLYSQIQMGAKLGMQTMEMSLAKLYEKGNVTWANAMAKAVKPDELEALIGPEPRETK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHCCCCCCHHH
AKTKAKARAH
HHHHHHHCCC
>Mature Secondary Structure 
SLMIEDVLESLVEQGGSDVHIQARAPIFFRINGQLTAQTQFGENMEPMVVQALIFQMLN
CCHHHHHHHHHHHCCCCEEEEEEECCEEEEECCEEEEEECCCCCCCHHHHHHHHHHHHH
NMQRKHLEQNWELDSAYGVKGLARFRLNVYRERGSWAACMRALASKIPNADKLGIPMILR
HHHHHHHHHCCCCCCHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHH
EMTERPRGLFLVTGQTGSGKTTTMAALIDLINRTRTEHILTVEDPIEYVFPNQKSLFHQR
HHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCHHHHCCCHHHHHHHH
QKGEDTKSFANALKGALRQDPDIILVGEMRDLETIGLACSAAETGHLVFGTLHTNSAAGT
HCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHEECCCCCCCEEEEEEECCCCHHH
VDRMLDVFPPEQQPQMRSQLANSIVCICSQNLVKKTGGGRCAAHEIMLNTPAIANLIRES
HHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHCCCCCEEHHHEEECCHHHHHHHHHC
KNSQLYSQIQMGAKLGMQTMEMSLAKLYEKGNVTWANAMAKAVKPDELEALIGPEPRETK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHCCCCCCHHH
AKTKAKARAH
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]