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The map label for this gene is trmB

Identifier: 113474688

GI number: 113474688

Start: 1384183

End: 1384845

Strand: Reverse

Name: trmB

Synonym: Tery_0871

Alternate gene names: 113474688

Gene position: 1384845-1384183 (Counterclockwise)

Preceding gene: 113474694

Following gene: 113474683

Centisome position: 17.87

GC content: 34.09

Gene sequence:

>663_bases
TTGGTAAGAGTAAGAATTAGACAGCATGTCAACCCTTTAGCTAGCAAATATCAACAGTCAGTAACTATCCCCAATTTTAG
TGATATTTATGCAGATATAAAGAAACCATTATATTTGGATATTGGTTGTGCTAGAGGGAGATTTTTATGGCAGATGGCAC
AGGTAGAACCTGAGTGGAATTTTCTGGGTTTAGAAATACGGGAACCCTTGGTGATAGAAGCTAATAATTGGCGAGATGAA
AAGGGTTTAAATAATCTGCATTATTTGTTTTGTAATGCGAATATTTCCCTGGGAAAAATATTTGAGTCTTTTCCAAAAGG
AATTTTAAAAAAAGTCTCTATTTTATTTCCAGATCCTTGGTTTAAAAGACGTCATCAAACCCGTCGAGTTGTACAACCAA
AATTAGTGGAAGATTTAGCAAATTATTTAACAGTAGGGGGAGAAGTTTTTCTGCATTCTGATATAGAAAAAGTAGCTAAG
GAAATGTGTAATCGCTTCTCGGCTCATGGTTCATTTTATAGAAGTTATAATGATTGGTTAAAAGAAAATATTCTGCCAGT
TTTATCAGAAAGAGAAAAGGTGACTTTAGGCAAAAATCAGCCTGTGTATCGGGCGTTATTTATTAAGTTAGAGGAACCTA
CTCCTAATATTATATCTCCTTGA

Upstream 100 bases:

>100_bases
ATTGGTATTAATTCCTTCAATAACAAACTTTTTCAGTAAGCTCTAATTATTAATCAGCTAAAGTCGAGAGAGATCGACTG
TAAGAATGGAGGCATAAAAT

Downstream 100 bases:

>100_bases
ATACTCACTGTGAGAAAATTTTATAGGATTGAGAATTGGGATAAATTAACATAATAAAAATATTTTTATTATGGTTAATT
AGAGTTTGCTAAAAAAGTTT

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 220; Mature: 220

Protein sequence:

>220_residues
MVRVRIRQHVNPLASKYQQSVTIPNFSDIYADIKKPLYLDIGCARGRFLWQMAQVEPEWNFLGLEIREPLVIEANNWRDE
KGLNNLHYLFCNANISLGKIFESFPKGILKKVSILFPDPWFKRRHQTRRVVQPKLVEDLANYLTVGGEVFLHSDIEKVAK
EMCNRFSAHGSFYRSYNDWLKENILPVLSEREKVTLGKNQPVYRALFIKLEEPTPNIISP

Sequences:

>Translated_220_residues
MVRVRIRQHVNPLASKYQQSVTIPNFSDIYADIKKPLYLDIGCARGRFLWQMAQVEPEWNFLGLEIREPLVIEANNWRDE
KGLNNLHYLFCNANISLGKIFESFPKGILKKVSILFPDPWFKRRHQTRRVVQPKLVEDLANYLTVGGEVFLHSDIEKVAK
EMCNRFSAHGSFYRSYNDWLKENILPVLSEREKVTLGKNQPVYRALFIKLEEPTPNIISP
>Mature_220_residues
MVRVRIRQHVNPLASKYQQSVTIPNFSDIYADIKKPLYLDIGCARGRFLWQMAQVEPEWNFLGLEIREPLVIEANNWRDE
KGLNNLHYLFCNANISLGKIFESFPKGILKKVSILFPDPWFKRRHQTRRVVQPKLVEDLANYLTVGGEVFLHSDIEKVAK
EMCNRFSAHGSFYRSYNDWLKENILPVLSEREKVTLGKNQPVYRALFIKLEEPTPNIISP

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Homo sapiens, GI167004078, Length=253, Percent_Identity=29.2490118577075, Blast_Score=81, Evalue=8e-16,
Organism=Escherichia coli, GI1789330, Length=135, Percent_Identity=35.5555555555556, Blast_Score=81, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17536689, Length=228, Percent_Identity=27.6315789473684, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320000, Length=238, Percent_Identity=28.5714285714286, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_TRIEI (Q117P8)

Other databases:

- EMBL:   CP000393
- RefSeq:   YP_720749.1
- ProteinModelPortal:   Q117P8
- SMR:   Q117P8
- STRING:   Q117P8
- GeneID:   4245417
- GenomeReviews:   CP000393_GR
- KEGG:   ter:Tery_0871
- NMPDR:   fig|203124.1.peg.3817
- eggNOG:   COG0220
- HOGENOM:   HBG284653
- OMA:   KRRVVQP
- PhylomeDB:   Q117P8
- ProtClustDB:   PRK00121
- BioCyc:   TERY203124:TERY_0871-MONOMER
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1
- TIGRFAMs:   TIGR00091

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 25717; Mature: 25717

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: NA

Important sites: BINDING 40-40 BINDING 65-65 BINDING 92-92 BINDING 118-118 BINDING 122-122 BINDING 154-154

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRVRIRQHVNPLASKYQQSVTIPNFSDIYADIKKPLYLDIGCARGRFLWQMAQVEPEWN
CEEEEHHHHCCHHHHHHHHCCCCCCHHHHHHHHCCCEEEEECCCCCHHHHHHHHCCCCCC
FLGLEIREPLVIEANNWRDEKGLNNLHYLFCNANISLGKIFESFPKGILKKVSILFPDPW
EEEEEECCCEEEECCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHH
FKRRHQTRRVVQPKLVEDLANYLTVGGEVFLHSDIEKVAKEMCNRFSAHGSFYRSYNDWL
HHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
KENILPVLSEREKVTLGKNQPVYRALFIKLEEPTPNIISP
HHCCHHHHCCCCEEEECCCCCEEEEEEEEECCCCCCCCCC
>Mature Secondary Structure
MVRVRIRQHVNPLASKYQQSVTIPNFSDIYADIKKPLYLDIGCARGRFLWQMAQVEPEWN
CEEEEHHHHCCHHHHHHHHCCCCCCHHHHHHHHCCCEEEEECCCCCHHHHHHHHCCCCCC
FLGLEIREPLVIEANNWRDEKGLNNLHYLFCNANISLGKIFESFPKGILKKVSILFPDPW
EEEEEECCCEEEECCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHH
FKRRHQTRRVVQPKLVEDLANYLTVGGEVFLHSDIEKVAKEMCNRFSAHGSFYRSYNDWL
HHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
KENILPVLSEREKVTLGKNQPVYRALFIKLEEPTPNIISP
HHCCHHHHCCCCEEEECCCCCEEEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA