Click here to switch to the map view.

The map label for this gene is speE [H]

Identifier: 113474566

GI number: 113474566

Start: 1168841

End: 1169767

Strand: Reverse

Name: speE [H]

Synonym: Tery_0722

Alternate gene names: 113474566

Gene position: 1169767-1168841 (Counterclockwise)

Preceding gene: 113474567

Following gene: 113474565

Centisome position: 15.09

GC content: 36.03

Gene sequence:

>927_bases
ATGGCAGGTACTAATATTAATACAGATTTGTATTTCACTGAGCAAATTACACCCTGGGACATTTATAAACATGGTATTGA
CCAGGTGCTTGTTTATCAAAAAACAAAATATCAGGAAATTTATATTGTCAAGGGTGTTTATGGTAAAGCTTTAATATTAG
ATGGTAAGTGGCAGTCTTGCACTGCAGATGAATTTCTCTACCATGAAGGCTTGGTACATCCAGCTATGATTTGTCATGGG
TCTCCACGAAAAGTCTTAGTATTTGGAGGAGCAGAAGGAGCAACAATACGAGAAGTATTACGCTGGAAAACAGTTGAGCA
AGTAGTTATGGTTGACATAGATGAGAAGGTTGTAGAAGCTTGCAAAAAGTATTTGCCTGAAATGCACCAAAATTCTTTTG
AAGACCCCCGTGTAGAGTTAATCATCGATGATGCTCAGAATTTTTTAGACTCTTCTAATGAAACTTGGGATGTAATTATT
TCTGACCTGACAGATCCTATTGAACATGGTCTTTCTTTTCCTTTATTTACTCAGGAATACTTTCAAAAAATTTCCCAAGT
TTTGTCGGCTTTAGGCTCGTTTATAATACAAGGGGGTTCAATTTCTCCTGCTGAGATGTATCTTCATGCTCGTGTAGTTA
AAACTTTACAAAGTGTATTTAATTGTGTTTATTCCCTGATACCTTATTCTACGAGTTATGGATGTCCATTAGGGTTTGTT
GTTTGTTCTGAGCAAAAATTTTCTTCTACACCAAACCCAGAAAAAGTAGACCGTTTATTGGCAGAAAAAACTACAGGCGG
TTTTAAGTTTATGGATGGTGTTTCTTTATTGGGTATGTTGCAAATACCTTTGAATTTACGACAGGCGATCGCTACAGAAA
CTCAGATATATACTTTGAAAACTCCACCAAAATCTATTCAGGTTTGA

Upstream 100 bases:

>100_bases
AGTTTGGTATAATTGATGGAATAAATATTTTTCTGGTTCCCTATTTTTTGACTCATTTTCCCTACTCCCTTTCAGTAAAT
TATCTGGAGACAAATAATCA

Downstream 100 bases:

>100_bases
GAGCAACATCTTTAATCATTTGCCAAAGTCTTGCTAAATATTCTTGAAAGTAGGGAGTAGATCTAACTACTAAATAGGTA
GGTGTAAAAAAAACTTAGCT

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY [H]

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MAGTNINTDLYFTEQITPWDIYKHGIDQVLVYQKTKYQEIYIVKGVYGKALILDGKWQSCTADEFLYHEGLVHPAMICHG
SPRKVLVFGGAEGATIREVLRWKTVEQVVMVDIDEKVVEACKKYLPEMHQNSFEDPRVELIIDDAQNFLDSSNETWDVII
SDLTDPIEHGLSFPLFTQEYFQKISQVLSALGSFIIQGGSISPAEMYLHARVVKTLQSVFNCVYSLIPYSTSYGCPLGFV
VCSEQKFSSTPNPEKVDRLLAEKTTGGFKFMDGVSLLGMLQIPLNLRQAIATETQIYTLKTPPKSIQV

Sequences:

>Translated_308_residues
MAGTNINTDLYFTEQITPWDIYKHGIDQVLVYQKTKYQEIYIVKGVYGKALILDGKWQSCTADEFLYHEGLVHPAMICHG
SPRKVLVFGGAEGATIREVLRWKTVEQVVMVDIDEKVVEACKKYLPEMHQNSFEDPRVELIIDDAQNFLDSSNETWDVII
SDLTDPIEHGLSFPLFTQEYFQKISQVLSALGSFIIQGGSISPAEMYLHARVVKTLQSVFNCVYSLIPYSTSYGCPLGFV
VCSEQKFSSTPNPEKVDRLLAEKTTGGFKFMDGVSLLGMLQIPLNLRQAIATETQIYTLKTPPKSIQV
>Mature_307_residues
AGTNINTDLYFTEQITPWDIYKHGIDQVLVYQKTKYQEIYIVKGVYGKALILDGKWQSCTADEFLYHEGLVHPAMICHGS
PRKVLVFGGAEGATIREVLRWKTVEQVVMVDIDEKVVEACKKYLPEMHQNSFEDPRVELIIDDAQNFLDSSNETWDVIIS
DLTDPIEHGLSFPLFTQEYFQKISQVLSALGSFIIQGGSISPAEMYLHARVVKTLQSVFNCVYSLIPYSTSYGCPLGFVV
CSEQKFSSTPNPEKVDRLLAEKTTGGFKFMDGVSLLGMLQIPLNLRQAIATETQIYTLKTPPKSIQV

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

Organism=Homo sapiens, GI63253298, Length=234, Percent_Identity=30.3418803418803, Blast_Score=126, Evalue=2e-29,
Organism=Escherichia coli, GI1786312, Length=176, Percent_Identity=42.6136363636364, Blast_Score=159, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI25154106, Length=244, Percent_Identity=31.9672131147541, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI212645616, Length=234, Percent_Identity=32.9059829059829, Blast_Score=123, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6325326, Length=234, Percent_Identity=37.1794871794872, Blast_Score=149, Evalue=6e-37,
Organism=Saccharomyces cerevisiae, GI6323175, Length=268, Percent_Identity=30.9701492537313, Blast_Score=135, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24645443, Length=253, Percent_Identity=32.4110671936759, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI21355941, Length=184, Percent_Identity=32.6086956521739, Blast_Score=92, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 34682; Mature: 34551

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS51006 SPERMIDINE_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGTNINTDLYFTEQITPWDIYKHGIDQVLVYQKTKYQEIYIVKGVYGKALILDGKWQSC
CCCCCCCCCEEEECCCCHHHHHHHCHHHEEEEECCCCCEEEEEECCCCCEEEECCCCCCC
TADEFLYHEGLVHPAMICHGSPRKVLVFGGAEGATIREVLRWKTVEQVVMVDIDEKVVEA
CHHHHHHHCCCCCHHHEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHEEEECCHHHHHH
CKKYLPEMHQNSFEDPRVELIIDDAQNFLDSSNETWDVIISDLTDPIEHGLSFPLFTQEY
HHHHHHHHHCCCCCCCCEEEEEECHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHH
FQKISQVLSALGSFIIQGGSISPAEMYLHARVVKTLQSVFNCVYSLIPYSTSYGCPLGFV
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEE
VCSEQKFSSTPNPEKVDRLLAEKTTGGFKFMDGVSLLGMLQIPLNLRQAIATETQIYTLK
EECCHHCCCCCCHHHHHHHHHHHCCCCEEHHHHHHHHHHHHCCHHHHHHHCCCCEEEEEE
TPPKSIQV
CCCCCCCC
>Mature Secondary Structure 
AGTNINTDLYFTEQITPWDIYKHGIDQVLVYQKTKYQEIYIVKGVYGKALILDGKWQSC
CCCCCCCCEEEECCCCHHHHHHHCHHHEEEEECCCCCEEEEEECCCCCEEEECCCCCCC
TADEFLYHEGLVHPAMICHGSPRKVLVFGGAEGATIREVLRWKTVEQVVMVDIDEKVVEA
CHHHHHHHCCCCCHHHEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHEEEECCHHHHHH
CKKYLPEMHQNSFEDPRVELIIDDAQNFLDSSNETWDVIISDLTDPIEHGLSFPLFTQEY
HHHHHHHHHCCCCCCCCEEEEEECHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHH
FQKISQVLSALGSFIIQGGSISPAEMYLHARVVKTLQSVFNCVYSLIPYSTSYGCPLGFV
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEE
VCSEQKFSSTPNPEKVDRLLAEKTTGGFKFMDGVSLLGMLQIPLNLRQAIATETQIYTLK
EECCHHCCCCCCHHHHHHHHHHHCCCCEEHHHHHHHHHHHHCCHHHHHHHCCCCEEEEEE
TPPKSIQV
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA