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The map label for this gene is gacS [H]

Identifier: 113474406

GI number: 113474406

Start: 858851

End: 860560

Strand: Reverse

Name: gacS [H]

Synonym: Tery_0544

Alternate gene names: 113474406

Gene position: 860560-858851 (Counterclockwise)

Preceding gene: 113474407

Following gene: 113474405

Centisome position: 11.1

GC content: 29.53

Gene sequence:

>1710_bases
ATGTCATTAACTTCCAGATCAAAAAAACAGTCTATCCACCAGCAAAAAAGTGGATTCAAACATTTGTTGAGTATTATAGG
AATCAGAAAAAAAATAGTATTCGGTTATGCTCTGGGAATCATTATTGTAATGTTAGGAGTTTTAACAGGGCAGATATTGG
AGTTCAATTATAAATATCAAATTAGAGGGAAGATAAAGCAAGGTCAACAAGAGGTAGACTTATTAACAAATCTTACAAAA
AAAGTATTAAAAATAATTATAATTCAGAAAGATGTTTCCCAAAAAGTTAATAACTGGAAAATTTTAGAAAAAAAAATCTC
CCTAGAATTATCAAGTTTTTATGAGGTTAATACAATTTTCAAACAACTTAAATTAACACTAGAAACCGGAGATAAAAACA
TCTCTAAAAATACAGATGATGTTCAGCTTGAAGAATTTATCCAAACCTATTTTAATCCTCTAAAAATTTATATTAATCAA
TTAGAAAACTTAGTTCAAGACATTAAACAAATACCGCCTCAAACAAAAGAAACAGTAGAAACTGCTTCATTGGAATCACT
GAATGTAATTTTTAATAGTCAAATTACAATACAACTACAAAACTTATCGCAAGATTCAGAACAATTAGTAATACTAGCTA
ATCAGAGAGTAGAAAAACAAAATAGGGATTATCAAAAAGTAGAAAAATTAGGAATATATATATTAATATGTAGTCTACTA
CTGTCGGCAATAACTACAGTAATAATAGCAATTTATACCACAAACTCGATCATAACTCCCTTAAAAGTAATAACTAAAGT
GGCTGAAGAAGTTACAGAACAAGCTAATTTTGAACTCCAACTTCCTGTAACTACAAATGATGAAATAGGAGAATTAACAG
AATCTTTAAATGAGTTAATTAGTAAAGTAGCAGAATACACAGAGCAACTCCAGGAAGCTAAACTCAAAGCAGAAGCAGCA
AACCGTTCCAAAAGTGCTTTTCTAGCTACCATGAGTCATGAACTACGTACACCTTTAAATGCAATTATTGGTTATAGTGA
TATCCTTTATGAGGATGTTCTAGAAGGTGGATATAAAGATTTTATTCCCGATATAGAAAGAATTAAGCTAGCAGGTAAAA
ACCTCCTAGAAATGATTAGTGATATCTTAGATATTTCAAAAATAGAAGCAGGTCAAGTTACACTTTATTTAGAGAGTATT
GAGGTTAATAAGCTAGTTAAAGATGTGTTCAGTACAGCTAAAAATATTACTGAAAAAAACCGTAATAAATTTGAATTAGT
TTTAGGAGAAAATCTTGGTAGTATGTATGCAGATATGCCAAAAGTGAAACAGGTACTGATAAATTTACTAAGTAATGCAG
CTAAGTTTACAGAAGATGGTACAATTACACTCAGTGTAGAAAAAGTTAAAAATAATCAACAAAAAAATAATCAAAATCAC
AATGCAAAGTCATTAAGTAATGAAAACTACCATATAGTTTTTCGGGTAATAGATACTGGTATTGGTATAACAGAAGAACA
GTTAAAACATATATTCCAACCTTTTACTCAAGCAGATACTTCTACTACTCGTAAGTATGGTGGTACTGGTTTAGGATTAG
CAATATGTAAACGTTTATGTGAGAGAATGGGAGCAGAAATTACTGTAAAAAGTCAATTAGGAAAAGGGTCTACATTTACT
GTTTGGTTTCCTGAAAAGGTATTAATATAA

Upstream 100 bases:

>100_bases
ATAATTTAAAAGGTTTTCAGCTTATTATTAACTGTTATCAATATTATTACAAGCAATGTCGGATGCTATATACGATGAGG
GCTAAGGAATACTAGAACAG

Downstream 100 bases:

>100_bases
CTAAAAAAATCAGGTTTAAAGCAACTCTATTCAAGAGGATAAAAAAATATTATCCGTACATATTTAAAACCTCAAGCTTC
ATTTAGAGTTAACTAAAATG

Product: periplasmic sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 569; Mature: 568

Protein sequence:

>569_residues
MSLTSRSKKQSIHQQKSGFKHLLSIIGIRKKIVFGYALGIIIVMLGVLTGQILEFNYKYQIRGKIKQGQQEVDLLTNLTK
KVLKIIIIQKDVSQKVNNWKILEKKISLELSSFYEVNTIFKQLKLTLETGDKNISKNTDDVQLEEFIQTYFNPLKIYINQ
LENLVQDIKQIPPQTKETVETASLESLNVIFNSQITIQLQNLSQDSEQLVILANQRVEKQNRDYQKVEKLGIYILICSLL
LSAITTVIIAIYTTNSIITPLKVITKVAEEVTEQANFELQLPVTTNDEIGELTESLNELISKVAEYTEQLQEAKLKAEAA
NRSKSAFLATMSHELRTPLNAIIGYSDILYEDVLEGGYKDFIPDIERIKLAGKNLLEMISDILDISKIEAGQVTLYLESI
EVNKLVKDVFSTAKNITEKNRNKFELVLGENLGSMYADMPKVKQVLINLLSNAAKFTEDGTITLSVEKVKNNQQKNNQNH
NAKSLSNENYHIVFRVIDTGIGITEEQLKHIFQPFTQADTSTTRKYGGTGLGLAICKRLCERMGAEITVKSQLGKGSTFT
VWFPEKVLI

Sequences:

>Translated_569_residues
MSLTSRSKKQSIHQQKSGFKHLLSIIGIRKKIVFGYALGIIIVMLGVLTGQILEFNYKYQIRGKIKQGQQEVDLLTNLTK
KVLKIIIIQKDVSQKVNNWKILEKKISLELSSFYEVNTIFKQLKLTLETGDKNISKNTDDVQLEEFIQTYFNPLKIYINQ
LENLVQDIKQIPPQTKETVETASLESLNVIFNSQITIQLQNLSQDSEQLVILANQRVEKQNRDYQKVEKLGIYILICSLL
LSAITTVIIAIYTTNSIITPLKVITKVAEEVTEQANFELQLPVTTNDEIGELTESLNELISKVAEYTEQLQEAKLKAEAA
NRSKSAFLATMSHELRTPLNAIIGYSDILYEDVLEGGYKDFIPDIERIKLAGKNLLEMISDILDISKIEAGQVTLYLESI
EVNKLVKDVFSTAKNITEKNRNKFELVLGENLGSMYADMPKVKQVLINLLSNAAKFTEDGTITLSVEKVKNNQQKNNQNH
NAKSLSNENYHIVFRVIDTGIGITEEQLKHIFQPFTQADTSTTRKYGGTGLGLAICKRLCERMGAEITVKSQLGKGSTFT
VWFPEKVLI
>Mature_568_residues
SLTSRSKKQSIHQQKSGFKHLLSIIGIRKKIVFGYALGIIIVMLGVLTGQILEFNYKYQIRGKIKQGQQEVDLLTNLTKK
VLKIIIIQKDVSQKVNNWKILEKKISLELSSFYEVNTIFKQLKLTLETGDKNISKNTDDVQLEEFIQTYFNPLKIYINQL
ENLVQDIKQIPPQTKETVETASLESLNVIFNSQITIQLQNLSQDSEQLVILANQRVEKQNRDYQKVEKLGIYILICSLLL
SAITTVIIAIYTTNSIITPLKVITKVAEEVTEQANFELQLPVTTNDEIGELTESLNELISKVAEYTEQLQEAKLKAEAAN
RSKSAFLATMSHELRTPLNAIIGYSDILYEDVLEGGYKDFIPDIERIKLAGKNLLEMISDILDISKIEAGQVTLYLESIE
VNKLVKDVFSTAKNITEKNRNKFELVLGENLGSMYADMPKVKQVLINLLSNAAKFTEDGTITLSVEKVKNNQQKNNQNHN
AKSLSNENYHIVFRVIDTGIGITEEQLKHIFQPFTQADTSTTRKYGGTGLGLAICKRLCERMGAEITVKSQLGKGSTFTV
WFPEKVLI

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=280, Percent_Identity=38.2142857142857, Blast_Score=167, Evalue=2e-42,
Organism=Escherichia coli, GI145693157, Length=280, Percent_Identity=37.8571428571429, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI87081816, Length=415, Percent_Identity=29.1566265060241, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI48994928, Length=246, Percent_Identity=33.3333333333333, Blast_Score=129, Evalue=5e-31,
Organism=Escherichia coli, GI1788713, Length=278, Percent_Identity=32.7338129496403, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1786600, Length=253, Percent_Identity=28.4584980237154, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1790436, Length=237, Percent_Identity=27.8481012658228, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1788393, Length=310, Percent_Identity=21.6129032258065, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1786912, Length=279, Percent_Identity=26.1648745519713, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1788549, Length=243, Percent_Identity=27.5720164609054, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790346, Length=243, Percent_Identity=24.6913580246914, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI87082128, Length=347, Percent_Identity=24.207492795389, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1790861, Length=329, Percent_Identity=26.4437689969605, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1786783, Length=343, Percent_Identity=23.0320699708455, Blast_Score=70, Evalue=5e-13,
Organism=Escherichia coli, GI1789808, Length=248, Percent_Identity=27.8225806451613, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1787894, Length=243, Percent_Identity=25.1028806584362, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1788279, Length=345, Percent_Identity=22.0289855072464, Blast_Score=64, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6322044, Length=75, Percent_Identity=42.6666666666667, Blast_Score=73, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 64291; Mature: 64160

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLTSRSKKQSIHQQKSGFKHLLSIIGIRKKIVFGYALGIIIVMLGVLTGQILEFNYKYQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEE
IRGKIKQGQQEVDLLTNLTKKVLKIIIIQKDVSQKVNNWKILEKKISLELSSFYEVNTIF
EECCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
KQLKLTLETGDKNISKNTDDVQLEEFIQTYFNPLKIYINQLENLVQDIKQIPPQTKETVE
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
TASLESLNVIFNSQITIQLQNLSQDSEQLVILANQRVEKQNRDYQKVEKLGIYILICSLL
HHHHHHHHEEECCEEEEEEECCCCCCCCEEEEECHHHHHHCCHHHHHHHHHHHHHHHHHH
LSAITTVIIAIYTTNSIITPLKVITKVAEEVTEQANFELQLPVTTNDEIGELTESLNELI
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHH
SKVAEYTEQLQEAKLKAEAANRSKSAFLATMSHELRTPLNAIIGYSDILYEDVLEGGYKD
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHCHHHHHHHHHHCCCHHH
FIPDIERIKLAGKNLLEMISDILDISKIEAGQVTLYLESIEVNKLVKDVFSTAKNITEKN
HCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHCC
RNKFELVLGENLGSMYADMPKVKQVLINLLSNAAKFTEDGTITLSVEKVKNNQQKNNQNH
CCEEEEEECCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHCCHHHCCCCC
NAKSLSNENYHIVFRVIDTGIGITEEQLKHIFQPFTQADTSTTRKYGGTGLGLAICKRLC
CHHHCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHH
ERMGAEITVKSQLGKGSTFTVWFPEKVLI
HHCCCCEEEECCCCCCCEEEEECCCHHCC
>Mature Secondary Structure 
SLTSRSKKQSIHQQKSGFKHLLSIIGIRKKIVFGYALGIIIVMLGVLTGQILEFNYKYQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEE
IRGKIKQGQQEVDLLTNLTKKVLKIIIIQKDVSQKVNNWKILEKKISLELSSFYEVNTIF
EECCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
KQLKLTLETGDKNISKNTDDVQLEEFIQTYFNPLKIYINQLENLVQDIKQIPPQTKETVE
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
TASLESLNVIFNSQITIQLQNLSQDSEQLVILANQRVEKQNRDYQKVEKLGIYILICSLL
HHHHHHHHEEECCEEEEEEECCCCCCCCEEEEECHHHHHHCCHHHHHHHHHHHHHHHHHH
LSAITTVIIAIYTTNSIITPLKVITKVAEEVTEQANFELQLPVTTNDEIGELTESLNELI
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHH
SKVAEYTEQLQEAKLKAEAANRSKSAFLATMSHELRTPLNAIIGYSDILYEDVLEGGYKD
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHCHHHHHHHHHHCCCHHH
FIPDIERIKLAGKNLLEMISDILDISKIEAGQVTLYLESIEVNKLVKDVFSTAKNITEKN
HCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHCC
RNKFELVLGENLGSMYADMPKVKQVLINLLSNAAKFTEDGTITLSVEKVKNNQQKNNQNH
CCEEEEEECCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHCCHHHCCCCC
NAKSLSNENYHIVFRVIDTGIGITEEQLKHIFQPFTQADTSTTRKYGGTGLGLAICKRLC
CHHHCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHH
ERMGAEITVKSQLGKGSTFTVWFPEKVLI
HHCCCCEEEECCCCCCCEEEEECCCHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]