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The map label for this gene is cobB [H]

Identifier: 113474382

GI number: 113474382

Start: 820641

End: 822119

Strand: Direct

Name: cobB [H]

Synonym: Tery_0517

Alternate gene names: 113474382

Gene position: 820641-822119 (Clockwise)

Preceding gene: 113474381

Following gene: 113474383

Centisome position: 10.59

GC content: 36.51

Gene sequence:

>1479_bases
TTGGGAACAGATATATTGAGTTTAATAATTGCGGGTGAGCGTAGTGGAGTTGGCAAAACAACTATTACCTTGGCGTTACT
AGCTTATTTAAAACGGCAAAATCTTAAGGTGCAATCATTTAAGGTGGGACCTGATTACATTGACCCGATGTTTCATCAAT
ATGTGACGGGTAAACCGTGTCGGAATTTAGACCCGGTGCTGACTTCTGAAGCTTATGTACAACAATGTTTTGAGCGTAAT
ATTCAGGATGTTGATTATGCTTTGGTGGAGGGAGTGATGGGTTTGTTTGATGGGGTGAGATCTTTGGCTCCCCCTTTACT
ACCATTAGAAAGTGAAACGGAAGATGATAGTGATGGGTCTTTCCCTGATTTTGCTTCTACTGCTCATATTTCCCGGTTGC
TGAATTTGCCTGTTTTGTTAGTGCTAGACTGCAGTCGGTTGTCTACTTCTGTGGCAGCGATCGCTCATGGTTTTGTTTCT
TTTGATCCTACTATTAATTTTGCTGGTCTGATACTTAACCGGGTAGGAAGCGATCGCCACTTACAACTACTACAAAATTC
TTTAAGGCATATAAGTTTACCTGTACTTGGGGTTTTTAGACGGCATCAAAATATTAGTATTCCTGAGAGACATTTAGGTT
TAATTCCCACTGATGAAATGCCGGAGTTAGATATTTTAATTAATGGCTTGGCTAATTTGGCAAAAACTTGTTTTAATTGG
CTAAAATTACTGGATTTTTTAAAGGTTAAAAATGAAATGTCTCAGCAGTTTTTATCTACTCAGGTTGGCTCTGAGCAGAA
AATACATTTACCGAGGTTAGTTGTGAATAGTGCTGAGAAAAAAAAGTCAGAAATTTTAATAGGGGTAGCTCGTGATCAAG
CATTTAATTTTTATTATCAAGACAACTTAGATATTTTAGAAGAGTTAGGAGCAAAAATAGTATTTTGGAGTCCTTTAAAA
GATAACAATTTACCAGTAGGAATACAAGGTTTATATTTTGGTGGTGGGTTTCCAGAAGTTTTTGCTCAACAGTTAAGTGA
AAATTTTCTGATGAGAAAAGCGGTTCAGGAAGCAATTATTTCCGGAATCCCTACTTATGCAGAATGTGGTGGGTTAATGT
ATTTATCTGAAGCAATTATTGATTTTAATAGTAACTCTTGGGAGATGGTAGGAATTTTGCCGACTAAGGCAGTAATGGGT
AAAAGTTTAAAGTTAGGATATCGGCAAGCAATTACTAATATTGATACTCCTCTTTTAAAAGCAAATACACTAGTTTATGG
TCATGAGTTTCATCGCTCAGAATTATATGAAGCACCCCTAATGCCACTATATAAAATGTGGCGAGTTTCTGAGAAAAAAA
ATCAGAGTCAAGCAACTTTTGAAGGCTGGAATTTTTATCAAGTTCATGCTTCTTATCTTCATCTTCATTGGGGAAATAAA
TTAGATATTCCGAGGCAGTTTCTCAGGCATTTTGAATAA

Upstream 100 bases:

>100_bases
CCCTGCAGTCGACGGTTATGGTTAAAGCAAGAATAAAGAACAAAGAAGTGATCATTAATTTAGATAATCTTATGTTTTCT
TGATTTTTTGTGATAGATTT

Downstream 100 bases:

>100_bases
GGCAGAAAAAAAGATTAAAGTATATTTTTCTGTAAATTAAGGGACAGTTTTCTATGGCTAATATCTGATATTTTATTCAT
GATCTAGCTATTAGCTATTT

Product: cobyrinic acid a,c-diamide synthase

Products: ADP; phosphate; Hydrogenobyrinic acid a,c diamide; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 492; Mature: 491

Protein sequence:

>492_residues
MGTDILSLIIAGERSGVGKTTITLALLAYLKRQNLKVQSFKVGPDYIDPMFHQYVTGKPCRNLDPVLTSEAYVQQCFERN
IQDVDYALVEGVMGLFDGVRSLAPPLLPLESETEDDSDGSFPDFASTAHISRLLNLPVLLVLDCSRLSTSVAAIAHGFVS
FDPTINFAGLILNRVGSDRHLQLLQNSLRHISLPVLGVFRRHQNISIPERHLGLIPTDEMPELDILINGLANLAKTCFNW
LKLLDFLKVKNEMSQQFLSTQVGSEQKIHLPRLVVNSAEKKKSEILIGVARDQAFNFYYQDNLDILEELGAKIVFWSPLK
DNNLPVGIQGLYFGGGFPEVFAQQLSENFLMRKAVQEAIISGIPTYAECGGLMYLSEAIIDFNSNSWEMVGILPTKAVMG
KSLKLGYRQAITNIDTPLLKANTLVYGHEFHRSELYEAPLMPLYKMWRVSEKKNQSQATFEGWNFYQVHASYLHLHWGNK
LDIPRQFLRHFE

Sequences:

>Translated_492_residues
MGTDILSLIIAGERSGVGKTTITLALLAYLKRQNLKVQSFKVGPDYIDPMFHQYVTGKPCRNLDPVLTSEAYVQQCFERN
IQDVDYALVEGVMGLFDGVRSLAPPLLPLESETEDDSDGSFPDFASTAHISRLLNLPVLLVLDCSRLSTSVAAIAHGFVS
FDPTINFAGLILNRVGSDRHLQLLQNSLRHISLPVLGVFRRHQNISIPERHLGLIPTDEMPELDILINGLANLAKTCFNW
LKLLDFLKVKNEMSQQFLSTQVGSEQKIHLPRLVVNSAEKKKSEILIGVARDQAFNFYYQDNLDILEELGAKIVFWSPLK
DNNLPVGIQGLYFGGGFPEVFAQQLSENFLMRKAVQEAIISGIPTYAECGGLMYLSEAIIDFNSNSWEMVGILPTKAVMG
KSLKLGYRQAITNIDTPLLKANTLVYGHEFHRSELYEAPLMPLYKMWRVSEKKNQSQATFEGWNFYQVHASYLHLHWGNK
LDIPRQFLRHFE
>Mature_491_residues
GTDILSLIIAGERSGVGKTTITLALLAYLKRQNLKVQSFKVGPDYIDPMFHQYVTGKPCRNLDPVLTSEAYVQQCFERNI
QDVDYALVEGVMGLFDGVRSLAPPLLPLESETEDDSDGSFPDFASTAHISRLLNLPVLLVLDCSRLSTSVAAIAHGFVSF
DPTINFAGLILNRVGSDRHLQLLQNSLRHISLPVLGVFRRHQNISIPERHLGLIPTDEMPELDILINGLANLAKTCFNWL
KLLDFLKVKNEMSQQFLSTQVGSEQKIHLPRLVVNSAEKKKSEILIGVARDQAFNFYYQDNLDILEELGAKIVFWSPLKD
NNLPVGIQGLYFGGGFPEVFAQQLSENFLMRKAVQEAIISGIPTYAECGGLMYLSEAIIDFNSNSWEMVGILPTKAVMGK
SLKLGYRQAITNIDTPLLKANTLVYGHEFHRSELYEAPLMPLYKMWRVSEKKNQSQATFEGWNFYQVHASYLHLHWGNKL
DIPRQFLRHFE

Specific function: Responsible for the amidation of carboxylic groups at position A and C of either cobyrinic acid or hydrogenobrynic acid. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation [H]

COG id: COG1797

COG function: function code H; Cobyrinic acid a,c-diamide synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR004484
- InterPro:   IPR017929
- InterPro:   IPR011698 [H]

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3 [H]

EC number: 6.3.5.9

Molecular weight: Translated: 55433; Mature: 55301

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00237 G_PROTEIN_RECEP_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTDILSLIIAGERSGVGKTTITLALLAYLKRQNLKVQSFKVGPDYIDPMFHQYVTGKPC
CCHHHEEHHEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHCCCCCC
RNLDPVLTSEAYVQQCFERNIQDVDYALVEGVMGLFDGVRSLAPPLLPLESETEDDSDGS
CCCCCHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
FPDFASTAHISRLLNLPVLLVLDCSRLSTSVAAIAHGFVSFDPTINFAGLILNRVGSDRH
CCCHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHH
LQLLQNSLRHISLPVLGVFRRHQNISIPERHLGLIPTDEMPELDILINGLANLAKTCFNW
HHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LKLLDFLKVKNEMSQQFLSTQVGSEQKIHLPRLVVNSAEKKKSEILIGVARDQAFNFYYQ
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCHHHCCCCEEEEEECCCEEEEEEC
DNLDILEELGAKIVFWSPLKDNNLPVGIQGLYFGGGFPEVFAQQLSENFLMRKAVQEAII
CCHHHHHHCCCEEEEECCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
SGIPTYAECGGLMYLSEAIIDFNSNSWEMVGILPTKAVMGKSLKLGYRQAITNIDTPLLK
HCCCCHHHHCCHHHHHHHHHCCCCCCEEEEEEECCHHHHCCHHHHHHHHHHHCCCCCCEE
ANTLVYGHEFHRSELYEAPLMPLYKMWRVSEKKNQSQATFEGWNFYQVHASYLHLHWGNK
CCEEEECCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEECCCCEEEEEEEEEEEECCCC
LDIPRQFLRHFE
CCHHHHHHHHCC
>Mature Secondary Structure 
GTDILSLIIAGERSGVGKTTITLALLAYLKRQNLKVQSFKVGPDYIDPMFHQYVTGKPC
CHHHEEHHEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHCCCCCC
RNLDPVLTSEAYVQQCFERNIQDVDYALVEGVMGLFDGVRSLAPPLLPLESETEDDSDGS
CCCCCHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
FPDFASTAHISRLLNLPVLLVLDCSRLSTSVAAIAHGFVSFDPTINFAGLILNRVGSDRH
CCCHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHH
LQLLQNSLRHISLPVLGVFRRHQNISIPERHLGLIPTDEMPELDILINGLANLAKTCFNW
HHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LKLLDFLKVKNEMSQQFLSTQVGSEQKIHLPRLVVNSAEKKKSEILIGVARDQAFNFYYQ
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCHHHCCCCEEEEEECCCEEEEEEC
DNLDILEELGAKIVFWSPLKDNNLPVGIQGLYFGGGFPEVFAQQLSENFLMRKAVQEAII
CCHHHHHHCCCEEEEECCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
SGIPTYAECGGLMYLSEAIIDFNSNSWEMVGILPTKAVMGKSLKLGYRQAITNIDTPLLK
HCCCCHHHHCCHHHHHHHHHCCCCCCEEEEEEECCHHHHCCHHHHHHHHHHHCCCCCCEE
ANTLVYGHEFHRSELYEAPLMPLYKMWRVSEKKNQSQATFEGWNFYQVHASYLHLHWGNK
CCEEEECCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEECCCCEEEEEEEEEEEECCCC
LDIPRQFLRHFE
CCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; hydrogenobyrinic acid; L-glutamine; H2O

Specific reaction: 2 ATP + hydrogenobyrinic acid + 2 L-glutamine + 2 H2O = 2 ADP + 2 phosphate + hydrogenobyrinic acid a,c-diamide + 2 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA