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The map label for this gene is fcl [C]

Identifier: 113474359

GI number: 113474359

Start: 786994

End: 787938

Strand: Direct

Name: fcl [C]

Synonym: Tery_0491

Alternate gene names: 113474359

Gene position: 786994-787938 (Clockwise)

Preceding gene: 113474358

Following gene: 113474360

Centisome position: 10.15

GC content: 35.66

Gene sequence:

>945_bases
ATGATTAAATTAGAGTTAGAAAACAAAAGAATTTTAGTAACCGGTGGAGCTGGTTTTCTTGGACGCCAAGTTGTAGATAG
ACTTCATAAAGCAGGAGCTAATCCTGATAATATTACTATACTGCGATCGCGTGATTATGACCTTCGGACTCTAGGAGCTT
GCCAAAAAGCTGTTCAAGAACAGGATATTATAATTCACCTTGCTGCTCATGTAGGAGGTATTGGTCTAAATAAGGAAAAA
CCTGCTGAATTATTTTACGATAACTTGATGATGGGAGCACAATTAATTGACTGTGCCCATAAAGCAAATGTAGAAAAGTT
TGTATGTGTAGGTTCAATATGTGCTTATCCTAAATTTACTCCTGTTCCTTTCAAAGAAGAAGACCTTTGGAATGGTTATC
CAGAAGAAACTAATGCTCCTTATGGTATTGCGAAGAAAGCTCTTTTAGTTCAACTGGAAGCTTATCGTCAACAGTATGGT
TTTAATGGTGTTTATCTTTTACCTGTTAACTTGTACGGACCTGAAGATAATTTTGATCCTAAAAGTTCTCATGTAATTCC
TGCTTTAATTCGGAAAGTACATGAAGCTCAAGTAGGAGGAAAGAAAGAATTATCTGTTTGGGGTGACGGTAGTCCGAGTC
GTGAATTTTTATATTCTACAGATGCGGCCAGAGGAATTGTTATGGCAACTCAATTTTATAATGAATCTGATCCTATTAAT
TTAGGAACTAATCATGAAGTAAAGATTCGTAATTTAGTAGAGTTAATTTGTGAGTTAATGGATTTTCAGGGAGAGTTAGT
TTGGGAAATTGATAAGCCAAATGGTCAACCTAGGCGATGTTTAGATACAAAAAAAGCCAAGGAAAAATTTGGTTTTGTTG
CTGAGGTAGATTTGAAGCAAGGGTTGAAGAATACAATTGAATGGTATCGCCAAAATGCTGATTAA

Upstream 100 bases:

>100_bases
ATTCAAATTGGTATGACAGAATTACTGTGGCGAATGTGGCAATGATTTTCATCTACAATACTTGAAGGCTTATGATTGGC
ATAAATCTCGGAGGCTTTTG

Downstream 100 bases:

>100_bases
GTTGAAAGGTATAGACTTTATTGTAATCAATTGATATAGTAAAAAGTTGGAGGTAATTTACTTTCCAGCTTTTTTCTATA
TAAAAGCTTTTATAGTTAAA

Product: NAD-dependent epimerase/dehydratase

Products: GDP-4-dehydro-6-deoxy-D-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MIKLELENKRILVTGGAGFLGRQVVDRLHKAGANPDNITILRSRDYDLRTLGACQKAVQEQDIIIHLAAHVGGIGLNKEK
PAELFYDNLMMGAQLIDCAHKANVEKFVCVGSICAYPKFTPVPFKEEDLWNGYPEETNAPYGIAKKALLVQLEAYRQQYG
FNGVYLLPVNLYGPEDNFDPKSSHVIPALIRKVHEAQVGGKKELSVWGDGSPSREFLYSTDAARGIVMATQFYNESDPIN
LGTNHEVKIRNLVELICELMDFQGELVWEIDKPNGQPRRCLDTKKAKEKFGFVAEVDLKQGLKNTIEWYRQNAD

Sequences:

>Translated_314_residues
MIKLELENKRILVTGGAGFLGRQVVDRLHKAGANPDNITILRSRDYDLRTLGACQKAVQEQDIIIHLAAHVGGIGLNKEK
PAELFYDNLMMGAQLIDCAHKANVEKFVCVGSICAYPKFTPVPFKEEDLWNGYPEETNAPYGIAKKALLVQLEAYRQQYG
FNGVYLLPVNLYGPEDNFDPKSSHVIPALIRKVHEAQVGGKKELSVWGDGSPSREFLYSTDAARGIVMATQFYNESDPIN
LGTNHEVKIRNLVELICELMDFQGELVWEIDKPNGQPRRCLDTKKAKEKFGFVAEVDLKQGLKNTIEWYRQNAD
>Mature_314_residues
MIKLELENKRILVTGGAGFLGRQVVDRLHKAGANPDNITILRSRDYDLRTLGACQKAVQEQDIIIHLAAHVGGIGLNKEK
PAELFYDNLMMGAQLIDCAHKANVEKFVCVGSICAYPKFTPVPFKEEDLWNGYPEETNAPYGIAKKALLVQLEAYRQQYG
FNGVYLLPVNLYGPEDNFDPKSSHVIPALIRKVHEAQVGGKKELSVWGDGSPSREFLYSTDAARGIVMATQFYNESDPIN
LGTNHEVKIRNLVELICELMDFQGELVWEIDKPNGQPRRCLDTKKAKEKFGFVAEVDLKQGLKNTIEWYRQNAD

Specific function: Putative nucleotide sugar epimerase/dehydrogenase [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fucose synthase family [H]

Homologues:

Organism=Homo sapiens, GI4507709, Length=309, Percent_Identity=34.9514563106796, Blast_Score=185, Evalue=4e-47,
Organism=Homo sapiens, GI7657641, Length=333, Percent_Identity=27.027027027027, Blast_Score=108, Evalue=9e-24,
Organism=Homo sapiens, GI42516563, Length=321, Percent_Identity=26.4797507788162, Blast_Score=84, Evalue=1e-16,
Organism=Escherichia coli, GI1788365, Length=321, Percent_Identity=36.1370716510903, Blast_Score=212, Evalue=3e-56,
Organism=Escherichia coli, GI48994969, Length=346, Percent_Identity=23.4104046242775, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1788353, Length=347, Percent_Identity=24.207492795389, Blast_Score=83, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17554428, Length=311, Percent_Identity=31.5112540192926, Blast_Score=163, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI115532424, Length=329, Percent_Identity=24.0121580547112, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI19922778, Length=316, Percent_Identity=30.6962025316456, Blast_Score=169, Evalue=2e-42,
Organism=Drosophila melanogaster, GI21356223, Length=316, Percent_Identity=25.3164556962025, Blast_Score=68, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 1.1.1.271

Molecular weight: Translated: 35283; Mature: 35283

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKLELENKRILVTGGAGFLGRQVVDRLHKAGANPDNITILRSRDYDLRTLGACQKAVQE
CEEEEECCCEEEEECCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHH
QDIIIHLAAHVGGIGLNKEKPAELFYDNLMMGAQLIDCAHKANVEKFVCVGSICAYPKFT
CCEEEEEEHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHEEEEHHHHCCCCCC
PVPFKEEDLWNGYPEETNAPYGIAKKALLVQLEAYRQQYGFNGVYLLPVNLYGPEDNFDP
CCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCC
KSSHVIPALIRKVHEAQVGGKKELSVWGDGSPSREFLYSTDAARGIVMATQFYNESDPIN
CCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEECCCCCCEEEEEECCCCCCCEE
LGTNHEVKIRNLVELICELMDFQGELVWEIDKPNGQPRRCLDTKKAKEKFGFVAEVDLKQ
CCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCEEEEHHHH
GLKNTIEWYRQNAD
HHHHHHHHHHCCCC
>Mature Secondary Structure
MIKLELENKRILVTGGAGFLGRQVVDRLHKAGANPDNITILRSRDYDLRTLGACQKAVQE
CEEEEECCCEEEEECCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHH
QDIIIHLAAHVGGIGLNKEKPAELFYDNLMMGAQLIDCAHKANVEKFVCVGSICAYPKFT
CCEEEEEEHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHEEEEHHHHCCCCCC
PVPFKEEDLWNGYPEETNAPYGIAKKALLVQLEAYRQQYGFNGVYLLPVNLYGPEDNFDP
CCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCC
KSSHVIPALIRKVHEAQVGGKKELSVWGDGSPSREFLYSTDAARGIVMATQFYNESDPIN
CCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEECCCCCCEEEEEECCCCCCCEE
LGTNHEVKIRNLVELICELMDFQGELVWEIDKPNGQPRRCLDTKKAKEKFGFVAEVDLKQ
CCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCEEEEHHHH
GLKNTIEWYRQNAD
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDP-L-fucose; NADP+

Specific reaction: GDP-L-fucose + NADP+ = GDP-4-dehydro-6-deoxy-D-mannose + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]