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The map label for this gene is galE [C]

Identifier: 113474276

GI number: 113474276

Start: 620588

End: 621844

Strand: Direct

Name: galE [C]

Synonym: Tery_0398

Alternate gene names: 113474276

Gene position: 620588-621844 (Clockwise)

Preceding gene: 113474273

Following gene: 113474277

Centisome position: 8.01

GC content: 36.67

Gene sequence:

>1257_bases
ATGAAAGTCCTAGTAATTGGTGGCGATGGCTACTGCGGTTGGGCAACCGCTCTTTATCTTTCCAATCAAGGTTATGAGGT
AGGTATTTTAGACAGTATGGTTAGGCGACACTGGGATCTACAACTTCAAGTAGAAACCCTCACACCTATTGCCCCTATTC
AACAACGCATCCAACGTTGGAAAGACCTCACAGGCAAAAAAATTGATCTATATATAGGAGATATTACCAACTACGATTTC
CTAAGTACAACACTACATCAGTTTGAACCAGAATCTATAGTTCACTTTGGTGAACAACGTTCTGCTCCATTTTCTATGAT
TGACCGGGAACATGCTGTTACGACTCAAGTTAATAATGTTGTTGGTACTCTCAATATTCTTTATGCTATGAAAGAGGATT
TTCCAGACTGCCATTTAGTTAAACTGGGAACTATGGGAGAGTATGGTACTCCTAATATAGATATTGAAGAGGGTTATATC
AAAATTGAACATAATGGACGCACGGATACTCTACCCTATCCAAAACAACCAGGGTCTTTCTATCATCTCAGTAAAGTTCA
CGATAGCCACAACATTCACTTTGCCTGCAAAATATGGGGTATCCGGGCTACGGACCTTAATCAAGGAATTGTATATGGTG
TTGCTCTAACTGGTCTTCTAAATGATGAAACAATCCAAGATGAACTTTTGATTAACCGTCTTGACTATGATGGAGTTTTT
GGTACAGCTCTCAATAGATTTTGTATTCAAGCAGCAATTGGCCATCCGCTAACTGTTTATGGTACAGGTGGACAAACTCG
TGGCTTTTTAGATATTAGAGATACTGTCCGTTGTATGGAAATAGCGATCGCAAACCCAGCACAACCAGGTGAATTCCGAG
TATTTAACCAATTTACTGAAATGTTTAGTGTACTAGACCTAGCAGAAATGGTAAAAACAGCGGGTAAAACTATGGATTTA
GATGTACAAATTAATCACTTGGATAATCCTAGAGTAGAGTTAGAGCAACATTATTTCAATGCTAAAAATACGAATTTATT
AGAGCTTGGTTTAAAACCTCATTATCTATCTGATTCTTTGTTAGATTCTTTGCTCAATTTTGCTATTAAGTATAAAACAA
GAGTAGATAAAAACCATATTTTACCTAAAGTCTCTTGGCATCGAGAAAAAACTCAACAATTAGATTCTGTAAAAAGTACA
TTAATATCCAAAACTGAGGAAAAAAACAAACAATCAGATTCTGTAAAAGTACAATAG

Upstream 100 bases:

>100_bases
AAAGCTTGAGTAGATTTATCGACAAAATTAGTAACTATCAAAAAGTGCTAATACCTGATACATTAACCAAATATTAGTAA
GCAAGATTCAAAGTAAATTT

Downstream 100 bases:

>100_bases
TATCTAAAACTGAATAACAAATCTATCTTCAATAGGTTTATACTCAATTGTTTTATTAGGGATGTTTTATGTAACGTCCC
TATATATTAGTATTTGGAAA

Product: UDP-sulfoquinovose synthase

Products: UDPglucoseal [C]

Alternate protein names: NAD-Dependent Epimerase/Dehydratase; Sulfolipid Biosynthesis Protein; NAD Dependent Epimerase/Dehydratase Family; Sulfolipid Biosynthesis Protein SqdB; Epimerase; UDP Sulfoquinovose Synthase; Nucleoside-Diphosphate-Sugar Epimerase; NAD Dependent Epimerase/Dehydratase Family Protein

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MKVLVIGGDGYCGWATALYLSNQGYEVGILDSMVRRHWDLQLQVETLTPIAPIQQRIQRWKDLTGKKIDLYIGDITNYDF
LSTTLHQFEPESIVHFGEQRSAPFSMIDREHAVTTQVNNVVGTLNILYAMKEDFPDCHLVKLGTMGEYGTPNIDIEEGYI
KIEHNGRTDTLPYPKQPGSFYHLSKVHDSHNIHFACKIWGIRATDLNQGIVYGVALTGLLNDETIQDELLINRLDYDGVF
GTALNRFCIQAAIGHPLTVYGTGGQTRGFLDIRDTVRCMEIAIANPAQPGEFRVFNQFTEMFSVLDLAEMVKTAGKTMDL
DVQINHLDNPRVELEQHYFNAKNTNLLELGLKPHYLSDSLLDSLLNFAIKYKTRVDKNHILPKVSWHREKTQQLDSVKST
LISKTEEKNKQSDSVKVQ

Sequences:

>Translated_418_residues
MKVLVIGGDGYCGWATALYLSNQGYEVGILDSMVRRHWDLQLQVETLTPIAPIQQRIQRWKDLTGKKIDLYIGDITNYDF
LSTTLHQFEPESIVHFGEQRSAPFSMIDREHAVTTQVNNVVGTLNILYAMKEDFPDCHLVKLGTMGEYGTPNIDIEEGYI
KIEHNGRTDTLPYPKQPGSFYHLSKVHDSHNIHFACKIWGIRATDLNQGIVYGVALTGLLNDETIQDELLINRLDYDGVF
GTALNRFCIQAAIGHPLTVYGTGGQTRGFLDIRDTVRCMEIAIANPAQPGEFRVFNQFTEMFSVLDLAEMVKTAGKTMDL
DVQINHLDNPRVELEQHYFNAKNTNLLELGLKPHYLSDSLLDSLLNFAIKYKTRVDKNHILPKVSWHREKTQQLDSVKST
LISKTEEKNKQSDSVKVQ
>Mature_418_residues
MKVLVIGGDGYCGWATALYLSNQGYEVGILDSMVRRHWDLQLQVETLTPIAPIQQRIQRWKDLTGKKIDLYIGDITNYDF
LSTTLHQFEPESIVHFGEQRSAPFSMIDREHAVTTQVNNVVGTLNILYAMKEDFPDCHLVKLGTMGEYGTPNIDIEEGYI
KIEHNGRTDTLPYPKQPGSFYHLSKVHDSHNIHFACKIWGIRATDLNQGIVYGVALTGLLNDETIQDELLINRLDYDGVF
GTALNRFCIQAAIGHPLTVYGTGGQTRGFLDIRDTVRCMEIAIANPAQPGEFRVFNQFTEMFSVLDLAEMVKTAGKTMDL
DVQINHLDNPRVELEQHYFNAKNTNLLELGLKPHYLSDSLLDSLLNFAIKYKTRVDKNHILPKVSWHREKTQQLDSVKST
LISKTEEKNKQSDSVKVQ

Specific function: Galactose metabolism; third step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 47431; Mature: 47431

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLVIGGDGYCGWATALYLSNQGYEVGILDSMVRRHWDLQLQVETLTPIAPIQQRIQRW
CEEEEEECCCCCHHEEEEEECCCCCEEEHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHH
KDLTGKKIDLYIGDITNYDFLSTTLHQFEPESIVHFGEQRSAPFSMIDREHAVTTQVNNV
HHCCCCEEEEEEECCCCCHHHHHHHHHCCCHHHHHCCCCCCCCHHHHCCCHHHHHHHHHH
VGTLNILYAMKEDFPDCHLVKLGTMGEYGTPNIDIEEGYIKIEHNGRTDTLPYPKQPGSF
HHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEEECCEEEEEECCCCCCCCCCCCCCCE
YHLSKVHDSHNIHFACKIWGIRATDLNQGIVYGVALTGLLNDETIQDELLINRLDYDGVF
EEEEECCCCCCEEEEEEEEEEEECCCCCCEEEEEEEEECCCCCHHHHHHHHHHCCCCCHH
GTALNRFCIQAAIGHPLTVYGTGGQTRGFLDIRDTVRCMEIAIANPAQPGEFRVFNQFTE
HHHHHHHHHHHHCCCCEEEEECCCCCCCEEEHHHHHHHHHHEECCCCCCCCCHHHHHHHH
MFSVLDLAEMVKTAGKTMDLDVQINHLDNPRVELEQHYFNAKNTNLLELGLKPHYLSDSL
HHHHHHHHHHHHHCCCEEEEEEEEECCCCCCEEHHHHHCCCCCCCEEEECCCCCCCCHHH
LDSLLNFAIKYKTRVDKNHILPKVSWHREKTQQLDSVKSTLISKTEEKNKQSDSVKVQ
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEC
>Mature Secondary Structure
MKVLVIGGDGYCGWATALYLSNQGYEVGILDSMVRRHWDLQLQVETLTPIAPIQQRIQRW
CEEEEEECCCCCHHEEEEEECCCCCEEEHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHH
KDLTGKKIDLYIGDITNYDFLSTTLHQFEPESIVHFGEQRSAPFSMIDREHAVTTQVNNV
HHCCCCEEEEEEECCCCCHHHHHHHHHCCCHHHHHCCCCCCCCHHHHCCCHHHHHHHHHH
VGTLNILYAMKEDFPDCHLVKLGTMGEYGTPNIDIEEGYIKIEHNGRTDTLPYPKQPGSF
HHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEEECCEEEEEECCCCCCCCCCCCCCCE
YHLSKVHDSHNIHFACKIWGIRATDLNQGIVYGVALTGLLNDETIQDELLINRLDYDGVF
EEEEECCCCCCEEEEEEEEEEEECCCCCCEEEEEEEEECCCCCHHHHHHHHHHCCCCCHH
GTALNRFCIQAAIGHPLTVYGTGGQTRGFLDIRDTVRCMEIAIANPAQPGEFRVFNQFTE
HHHHHHHHHHHHCCCCEEEEECCCCCCCEEEHHHHHHHHHHEECCCCCCCCCHHHHHHHH
MFSVLDLAEMVKTAGKTMDLDVQINHLDNPRVELEQHYFNAKNTNLLELGLKPHYLSDSL
HHHHHHHHHHHHHCCCEEEEEEEEECCCCCCEEHHHHHCCCCCCCEEEECCCCCCCCHHH
LDSLLNFAIKYKTRVDKNHILPKVSWHREKTQQLDSVKSTLISKTEEKNKQSDSVKVQ
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA