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The map label for this gene is fixL [H]

Identifier: 113474104

GI number: 113474104

Start: 307162

End: 308481

Strand: Direct

Name: fixL [H]

Synonym: Tery_0203

Alternate gene names: 113474104

Gene position: 307162-308481 (Clockwise)

Preceding gene: 113474103

Following gene: 113474105

Centisome position: 3.96

GC content: 33.79

Gene sequence:

>1320_bases
ATGACTCCGGAACAGTTTATCGAATTAGCGAGGGTCTTGCCTGAACCCTCTTTATTCCTTACTCAAAAAGGGGAAATATT
GGCCATGAATAAGTTAGCGTCTTCAATCTTTGGCTATCGGAGTAAAGACTTACAAGGGCAACAAATATATGATTTTGTCA
AAGAATCTGAAAATATTGTTACTGAATATTTACAAGCTTGTTCCAGGAATCGGAAAATGGTTATAGGTTCTTTAACAATT
TGCACCGCTGATGGAGCAAACGTTGTTTGTCGTTCTCGAGGGGCAGTTGTTGAACCAGCTTCTTCTGGGTTACCAGCTAA
AATTTTTTTACGTTTAGAAAAAAATAATAATGCCAATAATAACTTTATTATACTCAATCAAAAAATTGAACAATTAAAGT
CAGAAATTGGGCATCGTCGCAAAGCCGAACATCAGTTGAAGGACACTTTGAATAAGCTACAAAAAACTCAAATTCAATTG
ATACACCAAGAAAAACTTTCTGCTTTAGGTCAAATGGCGGCTGGTATTGCCCATGAAATCAATAACCCAGTAAGCTTTAT
TCATGGAAATATTTTACCCGCTAAAGAATATGCTGAAGATTTGTTAAGATTAATTCGATTATATCAGAAACACTATCCTA
ACCCAGTACCAGAAATTCAAGAAGAAATTGATGAAATTGATTTAGATTTCCTCGTAGAAGACTTGACAAAATTGCTGAAA
TCAATGAGTTTTGGAACAACAAGAATCTGTGAAATTGTCAAGTCCATGCGAACCTTTACCCGTTTAGATGAAGCTGAAAT
TAAACAAGTGAATATTCACGATGGTATAGATAGTACATTAATGATTCTTCAACATCGTTTGAAAGAGATGTCTAATTTAC
CACAAATTCAAGTTTTGAAAAACTATGAGGAAATACCTCAAATAGAATGCTATTCGGGACAACTTAACCAAGTTTTTATG
AATATTTTCAGCAACTCTATTGATGCATTACAAGAATGTTATGAGCAAAAAAGTATTCAAGAGATTAATGAAAATCCACC
TCAAATTAGAATTCAAACAGCAGTGTTAAATCATGATTGGGTTACTATCCGAATTGCGGATAATGGCACAGGAATAAATG
AAGAAGTCAAGTTAAAACTGTTTGATCCTTTTTTTACCACTAAAGATATTGGCAAAGGAACAGGATTAGGTTTATCAATT
AGTTATGGAATTATTGTAGAAAAACATGGTGGCAATATATTCTGTAATTCAGAAGTGGGGAAAGGGACAGAATTTGTTAT
TCAAATCCCCATCCAACTATCAATGATTCAAGCTGCATAG

Upstream 100 bases:

>100_bases
TGGCTGTCGGGTAGTGGTTTATTTAGAACTAACAGAAGATGCAGAAGAAGCTGAGGGGAGAGAGTATTTCAAATCCTAAT
GTAATTTTAAGGTGTTAAAA

Downstream 100 bases:

>100_bases
TACCAATTTACAATTCACATTAAGAGTAGGTACACAAATTTAATTACATATTTTGTCAAGCTGAATTTATTATACTGCAT
GTTTTACATACATAAAATTA

Product: PAS/PAC sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 439; Mature: 438

Protein sequence:

>439_residues
MTPEQFIELARVLPEPSLFLTQKGEILAMNKLASSIFGYRSKDLQGQQIYDFVKESENIVTEYLQACSRNRKMVIGSLTI
CTADGANVVCRSRGAVVEPASSGLPAKIFLRLEKNNNANNNFIILNQKIEQLKSEIGHRRKAEHQLKDTLNKLQKTQIQL
IHQEKLSALGQMAAGIAHEINNPVSFIHGNILPAKEYAEDLLRLIRLYQKHYPNPVPEIQEEIDEIDLDFLVEDLTKLLK
SMSFGTTRICEIVKSMRTFTRLDEAEIKQVNIHDGIDSTLMILQHRLKEMSNLPQIQVLKNYEEIPQIECYSGQLNQVFM
NIFSNSIDALQECYEQKSIQEINENPPQIRIQTAVLNHDWVTIRIADNGTGINEEVKLKLFDPFFTTKDIGKGTGLGLSI
SYGIIVEKHGGNIFCNSEVGKGTEFVIQIPIQLSMIQAA

Sequences:

>Translated_439_residues
MTPEQFIELARVLPEPSLFLTQKGEILAMNKLASSIFGYRSKDLQGQQIYDFVKESENIVTEYLQACSRNRKMVIGSLTI
CTADGANVVCRSRGAVVEPASSGLPAKIFLRLEKNNNANNNFIILNQKIEQLKSEIGHRRKAEHQLKDTLNKLQKTQIQL
IHQEKLSALGQMAAGIAHEINNPVSFIHGNILPAKEYAEDLLRLIRLYQKHYPNPVPEIQEEIDEIDLDFLVEDLTKLLK
SMSFGTTRICEIVKSMRTFTRLDEAEIKQVNIHDGIDSTLMILQHRLKEMSNLPQIQVLKNYEEIPQIECYSGQLNQVFM
NIFSNSIDALQECYEQKSIQEINENPPQIRIQTAVLNHDWVTIRIADNGTGINEEVKLKLFDPFFTTKDIGKGTGLGLSI
SYGIIVEKHGGNIFCNSEVGKGTEFVIQIPIQLSMIQAA
>Mature_438_residues
TPEQFIELARVLPEPSLFLTQKGEILAMNKLASSIFGYRSKDLQGQQIYDFVKESENIVTEYLQACSRNRKMVIGSLTIC
TADGANVVCRSRGAVVEPASSGLPAKIFLRLEKNNNANNNFIILNQKIEQLKSEIGHRRKAEHQLKDTLNKLQKTQIQLI
HQEKLSALGQMAAGIAHEINNPVSFIHGNILPAKEYAEDLLRLIRLYQKHYPNPVPEIQEEIDEIDLDFLVEDLTKLLKS
MSFGTTRICEIVKSMRTFTRLDEAEIKQVNIHDGIDSTLMILQHRLKEMSNLPQIQVLKNYEEIPQIECYSGQLNQVFMN
IFSNSIDALQECYEQKSIQEINENPPQIRIQTAVLNHDWVTIRIADNGTGINEEVKLKLFDPFFTTKDIGKGTGLGLSIS
YGIIVEKHGGNIFCNSEVGKGTEFVIQIPIQLSMIQAA

Specific function: Putative oxygen sensor; modulates the activity of fixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates fixJ [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=282, Percent_Identity=28.3687943262411, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI1790436, Length=286, Percent_Identity=26.9230769230769, Blast_Score=96, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 49638; Mature: 49507

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS50112 PAS ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPEQFIELARVLPEPSLFLTQKGEILAMNKLASSIFGYRSKDLQGQQIYDFVKESENIV
CCHHHHHHHHHHCCCCCEEEECCCCEEEHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
TEYLQACSRNRKMVIGSLTICTADGANVVCRSRGAVVEPASSGLPAKIFLRLEKNNNANN
HHHHHHHCCCCEEEEEEEEEEECCCCCEEEECCCCEECCCCCCCCEEEEEEEECCCCCCC
NFIILNQKIEQLKSEIGHRRKAEHQLKDTLNKLQKTQIQLIHQEKLSALGQMAAGIAHEI
CEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NNPVSFIHGNILPAKEYAEDLLRLIRLYQKHYPNPVPEIQEEIDEIDLDFLVEDLTKLLK
CCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHH
SMSFGTTRICEIVKSMRTFTRLDEAEIKQVNIHDGIDSTLMILQHRLKEMSNLPQIQVLK
HCCCCHHHHHHHHHHHHHHHHCCHHHHEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHH
NYEEIPQIECYSGQLNQVFMNIFSNSIDALQECYEQKSIQEINENPPQIRIQTAVLNHDW
CHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCE
VTIRIADNGTGINEEVKLKLFDPFFTTKDIGKGTGLGLSISYGIIVEKHGGNIFCNSEVG
EEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEEEEEEECCCEEEECCCCC
KGTEFVIQIPIQLSMIQAA
CCCEEEEEECCEEEEECCC
>Mature Secondary Structure 
TPEQFIELARVLPEPSLFLTQKGEILAMNKLASSIFGYRSKDLQGQQIYDFVKESENIV
CHHHHHHHHHHCCCCCEEEECCCCEEEHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
TEYLQACSRNRKMVIGSLTICTADGANVVCRSRGAVVEPASSGLPAKIFLRLEKNNNANN
HHHHHHHCCCCEEEEEEEEEEECCCCCEEEECCCCEECCCCCCCCEEEEEEEECCCCCCC
NFIILNQKIEQLKSEIGHRRKAEHQLKDTLNKLQKTQIQLIHQEKLSALGQMAAGIAHEI
CEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NNPVSFIHGNILPAKEYAEDLLRLIRLYQKHYPNPVPEIQEEIDEIDLDFLVEDLTKLLK
CCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHH
SMSFGTTRICEIVKSMRTFTRLDEAEIKQVNIHDGIDSTLMILQHRLKEMSNLPQIQVLK
HCCCCHHHHHHHHHHHHHHHHCCHHHHEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHH
NYEEIPQIECYSGQLNQVFMNIFSNSIDALQECYEQKSIQEINENPPQIRIQTAVLNHDW
CHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCE
VTIRIADNGTGINEEVKLKLFDPFFTTKDIGKGTGLGLSISYGIIVEKHGGNIFCNSEVG
EEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEEEEEEECCCEEEECCCCC
KGTEFVIQIPIQLSMIQAA
CCCEEEEEECCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2046550; 1766374 [H]