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The map label for this gene is gcvH

Identifier: 110835454

GI number: 110835454

Start: 2933679

End: 2934065

Strand: Reverse

Name: gcvH

Synonym: ABO_2593

Alternate gene names: 110835454

Gene position: 2934065-2933679 (Counterclockwise)

Preceding gene: 110835455

Following gene: 110835453

Centisome position: 94.04

GC content: 56.59

Gene sequence:

>387_bases
ATGAGCGAGATTCCAGCCGAGCTGCGTTATGCCTCCAGCCATGAGTGGGCCAAGGTAGAAGACGGTGTTGCCACGGTGGG
CATCAGCGACCACGCCCAAGATGCCATGGGCGATCTGGTCTATGTTGAGCTGCCGGAAGTGGGCCAGGTTGTGGCCGCTG
GCGATGAAACCGGGGTAGTGGAATCCGTAAAAGCGGCCTCCGACATCTACAGCCCGGTGTCTGGCGAAATCGTTGAAATC
AACGAAGCGCTAGAAGATGAGCCAGAGTTGGTTAATAACGTTCCTTATGAAGGTGGCTGGTTGTTCAAAGTGCAGCTCAC
CGACGAAGGAGAGCTGGACAGCCTGCTCACCGCCGACCAGTACCAGGCGCAGATCGATAGCGAATAA

Upstream 100 bases:

>100_bases
ACATACATTCTGTAGTCGCCATCGACACGGCTACTGAATACATTTCAGCGTGCATGCGTGAGGCGTGCCGCGAGCAAGCG
ACTCTTAACAGGAGAAAACA

Downstream 100 bases:

>100_bases
CGGTTCGCCGTTTTCGTTGCTACAAGCCTCAAGCTGTAAGCTAAACCCCGCCCGGTTTTCTTGGCTGCTTGACGCTTGCA
GCTAATCCCCGTCGTTTTTA

Product: glycine cleavage system H protein

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 128; Mature: 127

Protein sequence:

>128_residues
MSEIPAELRYASSHEWAKVEDGVATVGISDHAQDAMGDLVYVELPEVGQVVAAGDETGVVESVKAASDIYSPVSGEIVEI
NEALEDEPELVNNVPYEGGWLFKVQLTDEGELDSLLTADQYQAQIDSE

Sequences:

>Translated_128_residues
MSEIPAELRYASSHEWAKVEDGVATVGISDHAQDAMGDLVYVELPEVGQVVAAGDETGVVESVKAASDIYSPVSGEIVEI
NEALEDEPELVNNVPYEGGWLFKVQLTDEGELDSLLTADQYQAQIDSE
>Mature_127_residues
SEIPAELRYASSHEWAKVEDGVATVGISDHAQDAMGDLVYVELPEVGQVVAAGDETGVVESVKAASDIYSPVSGEIVEIN
EALEDEPELVNNVPYEGGWLFKVQLTDEGELDSLLTADQYQAQIDSE

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=119, Percent_Identity=52.1008403361345, Blast_Score=134, Evalue=2e-32,
Organism=Homo sapiens, GI89057342, Length=119, Percent_Identity=51.2605042016807, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1789271, Length=129, Percent_Identity=60.4651162790698, Blast_Score=165, Evalue=8e-43,
Organism=Caenorhabditis elegans, GI17551294, Length=113, Percent_Identity=53.0973451327434, Blast_Score=131, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI17507493, Length=112, Percent_Identity=50.8928571428571, Blast_Score=127, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6319272, Length=129, Percent_Identity=44.1860465116279, Blast_Score=106, Evalue=8e-25,
Organism=Drosophila melanogaster, GI17865652, Length=127, Percent_Identity=51.9685039370079, Blast_Score=132, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_ALCBS (Q0VLA7)

Other databases:

- EMBL:   AM286690
- RefSeq:   YP_694313.1
- ProteinModelPortal:   Q0VLA7
- SMR:   Q0VLA7
- STRING:   Q0VLA7
- GeneID:   4211689
- GenomeReviews:   AM286690_GR
- KEGG:   abo:ABO_2593
- NMPDR:   fig|393595.12.peg.2597
- eggNOG:   COG0509
- HOGENOM:   HBG693789
- OMA:   TSDHEWL
- PhylomeDB:   Q0VLA7
- BioCyc:   ABOR393595:ABO_2593-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 13784; Mature: 13653

Theoretical pI: Translated: 3.48; Mature: 3.48

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEIPAELRYASSHEWAKVEDGVATVGISDHAQDAMGDLVYVELPEVGQVVAAGDETGVV
CCCCCCCCEECCCCCCEECCCCEEEECCCCCCHHHHCCEEEEECCCCCEEEECCCCCCHH
ESVKAASDIYSPVSGEIVEINEALEDEPELVNNVPYEGGWLFKVQLTDEGELDSLLTADQ
HHHHHHHHHCCCCCCCEEEECHHCCCCCHHHHCCCCCCCEEEEEEECCCCCCCHHHCCCH
YQAQIDSE
HHEECCCC
>Mature Secondary Structure 
SEIPAELRYASSHEWAKVEDGVATVGISDHAQDAMGDLVYVELPEVGQVVAAGDETGVV
CCCCCCCEECCCCCCEECCCCEEEECCCCCCHHHHCCEEEEECCCCCEEEECCCCCCHH
ESVKAASDIYSPVSGEIVEINEALEDEPELVNNVPYEGGWLFKVQLTDEGELDSLLTADQ
HHHHHHHHHCCCCCCCEEEECHHCCCCCHHHHCCCCCCCEEEEEEECCCCCCCHHHCCCH
YQAQIDSE
HHEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA