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The map label for this gene is greB [H]

Identifier: 110835318

GI number: 110835318

Start: 2782159

End: 2782674

Strand: Reverse

Name: greB [H]

Synonym: ABO_2457

Alternate gene names: 110835318

Gene position: 2782674-2782159 (Counterclockwise)

Preceding gene: 110835319

Following gene: 110835317

Centisome position: 89.18

GC content: 56.2

Gene sequence:

>516_bases
ATGGGCCGCTGGCGACCGAAGGGCAAACCTGCCTCCAAGTACATCACCGTTGAGGGATACCATAAGCTCAACGATGAGCT
GCAATATTTATGGAAGCAGAAGCGCCCTGAGATCACCCAGTCGGTGCAGGAGGCGGCGGCCCAGGGCGATCGCTCGGAGA
ACGCAGAATACATCTACGGCAAGAAACAGCTGCGGGAGATTGATCGGCGGGTGCGTTTTCTTAGCAAGCGCCTGGATGAC
ATGACGGTGGTGGATCGGGTGCCGGAAGACCAAAGTCGGGTGTTCTTTGGTGCTTGGGTAGAGCTGGAAAATGACACAGG
GGACACTGAAACCTACCGGTTGGTGGGGCCAGATGAAACCGATGCGGGAAGAGGCTATATCAGTATTGATGCGCCATTGG
CGCGAGCCTTGCTCAAGAAACAGGTGGATGACGAAGTGGAGGTGCGAACCCCTGCTGGCGAGCGTTGTCTGTTTATCAAC
CGCATTTGGTACGAAGAGCCTGCACGTCGGGATTGA

Upstream 100 bases:

>100_bases
GCCCGCGGAGCAAGGAACGTTCTTTGTTCAGGACCCTTCTGGCAACACCTTGGAGTTCAAGGGGCTGCGCGAATTGCACC
GGATCTTCGAGGTATAACCC

Downstream 100 bases:

>100_bases
GCCTAGGCTTGTACAGGCGCGTATGAGCTGGTGGGCAGGCTCGCTTGCCGAAATAATAGAGGAATTCTCCGCCGATGAGC
GCTAACAACCGTATCTGGTT

Product: transcription elongation factor GreB

Products: NA

Alternate protein names: Transcript cleavage factor greB [H]

Number of amino acids: Translated: 171; Mature: 170

Protein sequence:

>171_residues
MGRWRPKGKPASKYITVEGYHKLNDELQYLWKQKRPEITQSVQEAAAQGDRSENAEYIYGKKQLREIDRRVRFLSKRLDD
MTVVDRVPEDQSRVFFGAWVELENDTGDTETYRLVGPDETDAGRGYISIDAPLARALLKKQVDDEVEVRTPAGERCLFIN
RIWYEEPARRD

Sequences:

>Translated_171_residues
MGRWRPKGKPASKYITVEGYHKLNDELQYLWKQKRPEITQSVQEAAAQGDRSENAEYIYGKKQLREIDRRVRFLSKRLDD
MTVVDRVPEDQSRVFFGAWVELENDTGDTETYRLVGPDETDAGRGYISIDAPLARALLKKQVDDEVEVRTPAGERCLFIN
RIWYEEPARRD
>Mature_170_residues
GRWRPKGKPASKYITVEGYHKLNDELQYLWKQKRPEITQSVQEAAAQGDRSENAEYIYGKKQLREIDRRVRFLSKRLDDM
TVVDRVPEDQSRVFFGAWVELENDTGDTETYRLVGPDETDAGRGYISIDAPLARALLKKQVDDEVEVRTPAGERCLFINR
IWYEEPARRD

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082261, Length=153, Percent_Identity=56.8627450980392, Blast_Score=179, Evalue=9e-47,
Organism=Escherichia coli, GI87082225, Length=153, Percent_Identity=37.2549019607843, Blast_Score=102, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR001437
- InterPro:   IPR022691
- InterPro:   IPR006358 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 19982; Mature: 19850

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS00829 GREAB_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRWRPKGKPASKYITVEGYHKLNDELQYLWKQKRPEITQSVQEAAAQGDRSENAEYIYG
CCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCEEEC
KKQLREIDRRVRFLSKRLDDMTVVDRVPEDQSRVFFGAWVELENDTGDTETYRLVGPDET
HHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCEEEEEEEEEEECCCCCCCEEEEECCCCC
DAGRGYISIDAPLARALLKKQVDDEVEVRTPAGERCLFINRIWYEEPARRD
CCCCEEEEECHHHHHHHHHHHCCCCEEEECCCCCEEEEEEEHHCCCCCCCC
>Mature Secondary Structure 
GRWRPKGKPASKYITVEGYHKLNDELQYLWKQKRPEITQSVQEAAAQGDRSENAEYIYG
CCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCEEEC
KKQLREIDRRVRFLSKRLDDMTVVDRVPEDQSRVFFGAWVELENDTGDTETYRLVGPDET
HHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCEEEEEEEEEEECCCCCCCEEEEECCCCC
DAGRGYISIDAPLARALLKKQVDDEVEVRTPAGERCLFINRIWYEEPARRD
CCCCEEEEECHHHHHHHHHHHCCCCEEEECCCCCEEEEEEEHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA