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The map label for this gene is dapF

Identifier: 110835193

GI number: 110835193

Start: 2659467

End: 2660312

Strand: Direct

Name: dapF

Synonym: ABO_2332

Alternate gene names: 110835193

Gene position: 2659467-2660312 (Clockwise)

Preceding gene: 110835189

Following gene: 110835194

Centisome position: 85.24

GC content: 60.87

Gene sequence:

>846_bases
ATGAACCTGCGCTTTACCAAAATGCACGGCCTGGGCAACGATTTCATGGTCATCGACGGCATTCGACAGTCATTGCCAGA
AACCGGCCTGCCTTTGCCTGCGGATGAAATACGCCGGCTGGCCGACCGCAAGTTTGGCGTGGGTTTTGACCAGATGCTAA
TCGTGCAGCCCGCCCAGGGCGATGCGGACTTCCGCTACCGGATCCTGAATGCCGATGGCTCCGAAGTGAGCCAATGCGGT
AATGGTGCCCGCTGCTTCGCCCGTTTTGTGCGCGAACAGGGACTCACCGACAAAAAATCTATTCGCGTGGAAACCGCTGC
AGGGCAAATGACCCTGTCTGTGACCGACGATGATCAGATCACCGTAGACATGGGCGCCCCTCGCTGGGCGCCAGACGCCA
TCCCCATGACCGCCAAAGCCGAAGCAGACAACTATTTTCTGGTGTCCGGCACCCAGGCCTGGGACGTAGGCGCCGTCGGC
CTGGGCAACCCACACTGCACCCTGCTGGTGGAAAATGTGGATACTGCCCCGGTGGATACCGTCGGCCCGCTACTGGAAAG
CCACGAACAATTTCCGGAACGGGCGAACGTGGGCTTTATGCAGATCGTCAGCCGCAACGAAATCCGCCTGCGCGTCTATG
AACGTGGCGCAGGGGAAACCCTGGCTTGCGGCTCCGGTGCCTGTGCCGCAGTGGTGATCGGCCAACGCCGGGACTGGCTG
GATGACACCGTCACCGTGCACCTACCCGGCGGCACCCTGCACGTCTGCTACGATGGGCAAGGCGGCATCCAGATGACAGG
CCCCGCCAGCCATGTCTATGATGGCGCTATTGAGATCGCGCTTTAG

Upstream 100 bases:

>100_bases
TTGAAACACCGCTAATCGCGCAGGCTCTCCGCCACCCAACCCCTGACGCCACCGCCACGGCAGAGTACAATGGCCTTTTA
CCCTGTAGCGGACACTTGCG

Downstream 100 bases:

>100_bases
CAAACGATTGAAAGCTCGGTGATGTCAGTGCCCACTGACCGGAACGAAAAACAAAGGACAGGATTGATGACGGATCAAAC
TCCAGCCAGCGCCGACCAGG

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MNLRFTKMHGLGNDFMVIDGIRQSLPETGLPLPADEIRRLADRKFGVGFDQMLIVQPAQGDADFRYRILNADGSEVSQCG
NGARCFARFVREQGLTDKKSIRVETAAGQMTLSVTDDDQITVDMGAPRWAPDAIPMTAKAEADNYFLVSGTQAWDVGAVG
LGNPHCTLLVENVDTAPVDTVGPLLESHEQFPERANVGFMQIVSRNEIRLRVYERGAGETLACGSGACAAVVIGQRRDWL
DDTVTVHLPGGTLHVCYDGQGGIQMTGPASHVYDGAIEIAL

Sequences:

>Translated_281_residues
MNLRFTKMHGLGNDFMVIDGIRQSLPETGLPLPADEIRRLADRKFGVGFDQMLIVQPAQGDADFRYRILNADGSEVSQCG
NGARCFARFVREQGLTDKKSIRVETAAGQMTLSVTDDDQITVDMGAPRWAPDAIPMTAKAEADNYFLVSGTQAWDVGAVG
LGNPHCTLLVENVDTAPVDTVGPLLESHEQFPERANVGFMQIVSRNEIRLRVYERGAGETLACGSGACAAVVIGQRRDWL
DDTVTVHLPGGTLHVCYDGQGGIQMTGPASHVYDGAIEIAL
>Mature_281_residues
MNLRFTKMHGLGNDFMVIDGIRQSLPETGLPLPADEIRRLADRKFGVGFDQMLIVQPAQGDADFRYRILNADGSEVSQCG
NGARCFARFVREQGLTDKKSIRVETAAGQMTLSVTDDDQITVDMGAPRWAPDAIPMTAKAEADNYFLVSGTQAWDVGAVG
LGNPHCTLLVENVDTAPVDTVGPLLESHEQFPERANVGFMQIVSRNEIRLRVYERGAGETLACGSGACAAVVIGQRRDWL
DDTVTVHLPGGTLHVCYDGQGGIQMTGPASHVYDGAIEIAL

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=279, Percent_Identity=57.7060931899642, Blast_Score=330, Evalue=7e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_ALCBS (Q0VM18)

Other databases:

- EMBL:   AM286690
- RefSeq:   YP_694052.1
- ProteinModelPortal:   Q0VM18
- SMR:   Q0VM18
- STRING:   Q0VM18
- GeneID:   4213290
- GenomeReviews:   AM286690_GR
- KEGG:   abo:ABO_2332
- NMPDR:   fig|393595.12.peg.2333
- eggNOG:   COG0253
- HOGENOM:   HBG399442
- OMA:   HTGIGFD
- PhylomeDB:   Q0VM18
- BioCyc:   ABOR393595:ABO_2332-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 30364; Mature: 30364

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 79-79 ACT_SITE 223-223

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLRFTKMHGLGNDFMVIDGIRQSLPETGLPLPADEIRRLADRKFGVGFDQMLIVQPAQG
CCEEEEEECCCCCCEEEEECHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCC
DADFRYRILNADGSEVSQCGNGARCFARFVREQGLTDKKSIRVETAAGQMTLSVTDDDQI
CCCEEEEEECCCCHHHHHCCCHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEECCCCEE
TVDMGAPRWAPDAIPMTAKAEADNYFLVSGTQAWDVGAVGLGNPHCTLLVENVDTAPVDT
EEECCCCCCCCCCCCEEEECCCCCEEEEECCCCCCCCEECCCCCEEEEEEECCCCCCCHH
VGPLLESHEQFPERANVGFMQIVSRNEIRLRVYERGAGETLACGSGACAAVVIGQRRDWL
HHHHHHHHHHCHHHCCCCEEEEECCCCEEEEEEECCCCCEEECCCCCEEEEEEECCCCCC
DDTVTVHLPGGTLHVCYDGQGGIQMTGPASHVYDGAIEIAL
CCEEEEEECCCEEEEEECCCCCEEECCCCCHHCCCEEEEEC
>Mature Secondary Structure
MNLRFTKMHGLGNDFMVIDGIRQSLPETGLPLPADEIRRLADRKFGVGFDQMLIVQPAQG
CCEEEEEECCCCCCEEEEECHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCC
DADFRYRILNADGSEVSQCGNGARCFARFVREQGLTDKKSIRVETAAGQMTLSVTDDDQI
CCCEEEEEECCCCHHHHHCCCHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEECCCCEE
TVDMGAPRWAPDAIPMTAKAEADNYFLVSGTQAWDVGAVGLGNPHCTLLVENVDTAPVDT
EEECCCCCCCCCCCCEEEECCCCCEEEEECCCCCCCCEECCCCCEEEEEEECCCCCCCHH
VGPLLESHEQFPERANVGFMQIVSRNEIRLRVYERGAGETLACGSGACAAVVIGQRRDWL
HHHHHHHHHHCHHHCCCCEEEEECCCCEEEEEEECCCCCEEECCCCCEEEEEEECCCCCC
DDTVTVHLPGGTLHVCYDGQGGIQMTGPASHVYDGAIEIAL
CCEEEEEECCCEEEEEECCCCCEEECCCCCHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA