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The map label for this gene is rpoD [H]

Identifier: 110834924

GI number: 110834924

Start: 2350147

End: 2352048

Strand: Direct

Name: rpoD [H]

Synonym: ABO_2063

Alternate gene names: 110834924

Gene position: 2350147-2352048 (Clockwise)

Preceding gene: 110834923

Following gene: 110834925

Centisome position: 75.32

GC content: 53.52

Gene sequence:

>1902_bases
ATGACGACTCAAAAGCAGCAGCAACAATCTCAGCTGAAGCAGTTGATCGCACTAGGTAAGGAACAGGGCTACCTTACCTA
CGCTGAAGTGAATGACCACTTGCCGGACTCCATTACGGATACCGATCAAGTAGAAGACATCATTCAGATGATCAACGACA
TGGGCATCACCGTGGTTGAAAAAGCCGCCGATGCCGACCAGTTGACGATCGACGAAAGCGCCGACAGCACAGACGAAGTC
GCCGCTGAAGAAGCCGCTGCCGTGCTCGCCTCCGTAGAGAACGAACCCGGTCGCACCACCGACCCGGTACGCATGTACAT
GCGCGAAATGGGCACCGTGGAGCTGCTCACTCGGGAAGGCGAAATCGAAATTGCCAAGCGCATTGAAGAAGGCACCCGTG
AAGTTCTTCACGCCCTCGCCTTCTGGCCCGGCGCTGTCGATATGGTGATTGAAGAATACGCCCGCGTGGAAAACGAAGAA
CGTAAGCTCACCGACATCATTGCCGGCTACTTGGATCCCAATGACGATGGCGCGGCAGCGACCAACGCCAATCGCGATCC
TGCTTTTGCTGAAAAAGCCAAGAAGGCGGCAGAAGAAAAAGCTAAGGCAAAAGAAAAAGCCAAAGAAGAAGGTTCTGACG
ATAAAGACAGCGACGACGAAGACGAAGAAGATGAAGGCGGCTTGGATCCGGTATTGGCCGCAGAACGCTTCGAGACGCTG
AAAAAACTGCAAGAGAAAGCAGAACGCTCTCTCAAGCGTAACGGTCGGGACCATGCATCCACCGCCAAGGCATTAGAAGC
GCTGGCAGAACACTTCACCCTGTTTAAACTGGTGCCGCGTATTTTCGACAGCATGCTCGTTGGTGCACGCGACAACCTGG
ACCTGGTACGTAAATTCGAGCGCCAAATCATGTCCGCCACCATCCGCCGTGGCGGTATGGATCGTAAGGATTTCATCAAG
GCATTTCCTGGCAACGAAACCAACATGGAGTGGATCGATAGCCAGCTGAAAACCAAAAAAGGCAAAGCCATCGCCGATAA
ACTGAGCGTCGTCAAAGAAGACGTTCAGCGTGCCCAGCGTAAAATCAGCACTATCGAAGACATCATTGGCTTGCCCGTTG
CCGACATCAAGGAAATCAACCGTCGCATTTCCATTGGCGAAGCAAAAGCGCGTCGCGCCAAGAAAGAAATGGTCGAAGCC
AACCTGCGTCTGGTTATTTCTATCGCCAAAAAATACACCAACCGAGGGCTACAATTCCTCGACCTGATTCAGGAAGGCAA
CATCGGCCTGATGAAAGCCGTAGACAAGTTCGAATACCGCCGTGGTTACAAATTCTCCACCTACGCTACTTGGTGGATTC
GTCAGGCCATCACACGCTCCATCGCCGACCAGGCACGCACCATCCGTATCCCGGTACACATGATAGAGACCATCAACAAG
ATCAACCGGGTACAACGTCAAATGCTGCAGGAAATGGGCCGCGAACCCACTCCAGAAGAGCTGGGTGTACGCTTGGAAAT
GCCGGAAGATAAAGTTCGTAAAGTACTCAAGATTGCCAAAGAGCCAATCTCCATGGAAACCCCCATCGGTGACGATGAAG
ATTCCAGCCTCGGCGACTTTATCGAAGACGGCAACATGGAATCCCCGGTAGATTCCGCCACCTCGCTTGGCCTGGAAGAA
GCCACCCGCGACGTACTGGCCAACCTCACCGCTCGGGAAGCTAAAGTCCTGCGCATGCGTTTCGGTATCGACATGAACAC
TGACCACACCCTTGAGGAAGTGGGAAAACAGTTCGACGTAACCCGTGAACGTATTCGTCAGATCGAAGCCAAGGCACTGC
GCAAACTCCGGCATCCGAGCCGCAGCGAGCACCTACGCAGCTTCCTCGACGGCGACCTGTAA

Upstream 100 bases:

>100_bases
GGCCGTAGACCCCATATCCCAGTATAATACGCCGTTTTCCCACGCCACCATCACGCTGGCAAGCGCAAGCTCCCAGCACG
ACGTTACGGGCTTTAATTGC

Downstream 100 bases:

>100_bases
AACCGGCAAATGCAAAATGCCTGGGCTGCGTTGCAGCCTGGGCAATAATAAAAAAGAGTACAGCCTTTCCCTAAACCAAA
TACACGTTATAATCTTCGCC

Product: RNA polymerase sigma factor RpoD

Products: NA

Alternate protein names: Sigma-70 [H]

Number of amino acids: Translated: 633; Mature: 632

Protein sequence:

>633_residues
MTTQKQQQQSQLKQLIALGKEQGYLTYAEVNDHLPDSITDTDQVEDIIQMINDMGITVVEKAADADQLTIDESADSTDEV
AAEEAAAVLASVENEPGRTTDPVRMYMREMGTVELLTREGEIEIAKRIEEGTREVLHALAFWPGAVDMVIEEYARVENEE
RKLTDIIAGYLDPNDDGAAATNANRDPAFAEKAKKAAEEKAKAKEKAKEEGSDDKDSDDEDEEDEGGLDPVLAAERFETL
KKLQEKAERSLKRNGRDHASTAKALEALAEHFTLFKLVPRIFDSMLVGARDNLDLVRKFERQIMSATIRRGGMDRKDFIK
AFPGNETNMEWIDSQLKTKKGKAIADKLSVVKEDVQRAQRKISTIEDIIGLPVADIKEINRRISIGEAKARRAKKEMVEA
NLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINK
INRVQRQMLQEMGREPTPEELGVRLEMPEDKVRKVLKIAKEPISMETPIGDDEDSSLGDFIEDGNMESPVDSATSLGLEE
ATRDVLANLTAREAKVLRMRFGIDMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEHLRSFLDGDL

Sequences:

>Translated_633_residues
MTTQKQQQQSQLKQLIALGKEQGYLTYAEVNDHLPDSITDTDQVEDIIQMINDMGITVVEKAADADQLTIDESADSTDEV
AAEEAAAVLASVENEPGRTTDPVRMYMREMGTVELLTREGEIEIAKRIEEGTREVLHALAFWPGAVDMVIEEYARVENEE
RKLTDIIAGYLDPNDDGAAATNANRDPAFAEKAKKAAEEKAKAKEKAKEEGSDDKDSDDEDEEDEGGLDPVLAAERFETL
KKLQEKAERSLKRNGRDHASTAKALEALAEHFTLFKLVPRIFDSMLVGARDNLDLVRKFERQIMSATIRRGGMDRKDFIK
AFPGNETNMEWIDSQLKTKKGKAIADKLSVVKEDVQRAQRKISTIEDIIGLPVADIKEINRRISIGEAKARRAKKEMVEA
NLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINK
INRVQRQMLQEMGREPTPEELGVRLEMPEDKVRKVLKIAKEPISMETPIGDDEDSSLGDFIEDGNMESPVDSATSLGLEE
ATRDVLANLTAREAKVLRMRFGIDMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEHLRSFLDGDL
>Mature_632_residues
TTQKQQQQSQLKQLIALGKEQGYLTYAEVNDHLPDSITDTDQVEDIIQMINDMGITVVEKAADADQLTIDESADSTDEVA
AEEAAAVLASVENEPGRTTDPVRMYMREMGTVELLTREGEIEIAKRIEEGTREVLHALAFWPGAVDMVIEEYARVENEER
KLTDIIAGYLDPNDDGAAATNANRDPAFAEKAKKAAEEKAKAKEKAKEEGSDDKDSDDEDEEDEGGLDPVLAAERFETLK
KLQEKAERSLKRNGRDHASTAKALEALAEHFTLFKLVPRIFDSMLVGARDNLDLVRKFERQIMSATIRRGGMDRKDFIKA
FPGNETNMEWIDSQLKTKKGKAIADKLSVVKEDVQRAQRKISTIEDIIGLPVADIKEINRRISIGEAKARRAKKEMVEAN
LRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKI
NRVQRQMLQEMGREPTPEELGVRLEMPEDKVRKVLKIAKEPISMETPIGDDEDSSLGDFIEDGNMESPVDSATSLGLEEA
TRDVLANLTAREAKVLRMRFGIDMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEHLRSFLDGDL

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=627, Percent_Identity=63.317384370016, Blast_Score=749, Evalue=0.0,
Organism=Escherichia coli, GI1789098, Length=245, Percent_Identity=41.6326530612245, Blast_Score=207, Evalue=2e-54,
Organism=Escherichia coli, GI1789871, Length=246, Percent_Identity=28.8617886178862, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1788231, Length=202, Percent_Identity=26.7326732673267, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007631
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04546 Sigma70_ner; PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 71543; Mature: 71412

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQKQQQQSQLKQLIALGKEQGYLTYAEVNDHLPDSITDTDQVEDIIQMINDMGITVVE
CCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCHHHH
KAADADQLTIDESADSTDEVAAEEAAAVLASVENEPGRTTDPVRMYMREMGTVELLTREG
HHCCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHCCC
EIEIAKRIEEGTREVLHALAFWPGAVDMVIEEYARVENEERKLTDIIAGYLDPNDDGAAA
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCC
TNANRDPAFAEKAKKAAEEKAKAKEKAKEEGSDDKDSDDEDEEDEGGLDPVLAAERFETL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
KKLQEKAERSLKRNGRDHASTAKALEALAEHFTLFKLVPRIFDSMLVGARDNLDLVRKFE
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
RQIMSATIRRGGMDRKDFIKAFPGNETNMEWIDSQLKTKKGKAIADKLSVVKEDVQRAQR
HHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
KISTIEDIIGLPVADIKEINRRISIGEAKARRAKKEMVEANLRLVISIAKKYTNRGLQFL
HHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
DLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINK
HHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHH
INRVQRQMLQEMGREPTPEELGVRLEMPEDKVRKVLKIAKEPISMETPIGDDEDSSLGDF
HHHHHHHHHHHHCCCCCHHHCCEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHH
IEDGNMESPVDSATSLGLEEATRDVLANLTAREAKVLRMRFGIDMNTDHTLEEVGKQFDV
HHCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
TRERIRQIEAKALRKLRHPSRSEHLRSFLDGDL
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCC
>Mature Secondary Structure 
TTQKQQQQSQLKQLIALGKEQGYLTYAEVNDHLPDSITDTDQVEDIIQMINDMGITVVE
CCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCHHHH
KAADADQLTIDESADSTDEVAAEEAAAVLASVENEPGRTTDPVRMYMREMGTVELLTREG
HHCCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHCCC
EIEIAKRIEEGTREVLHALAFWPGAVDMVIEEYARVENEERKLTDIIAGYLDPNDDGAAA
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCC
TNANRDPAFAEKAKKAAEEKAKAKEKAKEEGSDDKDSDDEDEEDEGGLDPVLAAERFETL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
KKLQEKAERSLKRNGRDHASTAKALEALAEHFTLFKLVPRIFDSMLVGARDNLDLVRKFE
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
RQIMSATIRRGGMDRKDFIKAFPGNETNMEWIDSQLKTKKGKAIADKLSVVKEDVQRAQR
HHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
KISTIEDIIGLPVADIKEINRRISIGEAKARRAKKEMVEANLRLVISIAKKYTNRGLQFL
HHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
DLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINK
HHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHH
INRVQRQMLQEMGREPTPEELGVRLEMPEDKVRKVLKIAKEPISMETPIGDDEDSSLGDF
HHHHHHHHHHHHCCCCCHHHCCEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHH
IEDGNMESPVDSATSLGLEEATRDVLANLTAREAKVLRMRFGIDMNTDHTLEEVGKQFDV
HHCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
TRERIRQIEAKALRKLRHPSRSEHLRSFLDGDL
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]