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The map label for this gene is prfC [H]

Identifier: 110636383

GI number: 110636383

Start: 4369227

End: 4370825

Strand: Reverse

Name: prfC [H]

Synonym: Meso_4059

Alternate gene names: 110636383

Gene position: 4370825-4369227 (Counterclockwise)

Preceding gene: 110636389

Following gene: 110636381

Centisome position: 99.06

GC content: 62.16

Gene sequence:

>1599_bases
ATGCCAGAGAGTTTGGCCGACGCTGTGGCGCGCCGCCGTACCTTCGCGATCATTTCGCACCCCGACGCGGGCAAAACGAC
GCTCACCGAAAAGCTGCTGCTTTTCGGCGGGGCCATCCAGCTTGCCGGCGAGGTGAAGGCAAAGAAGAACCGTATCCAGA
CCCGCTCGGACTGGATGGCGATCGAGCGCGAGCGCGGCATCTCGGTCGTCACCTCGGTGATGACCTTCGAATATGGCGGC
CACGTCTTCAACCTGCTCGACACGCCCGGCCACGAGGATTTCGCCGACGACACCTATCGCACGCTAACGGCGGTCGACTC
CGCCGTTATGGTGATCGACGCAGCAAAGGGCATCGAGCCGCGCACGCTGAAACTCTTCGAGGTGTGCCGGCTGCGGGACA
TTCCCATCATTACCTTCGTCAACAAGCTCGACCGGGAGAGCCGCGATCCGTTCGAGATCCTAGACGAGATCGAACAGAAG
CTGGCACTCGATACGGCGCCCATCACCTGGCCGATCGGAAGGGGAAAAAGCTTTTCGGGAACCTATCACCTGGCGAACAA
CACGATCCGGCACTCGGACGACGATGTGGAGCCGATCAGGGTGAACGGGCCGGAAGCGGAAGCCGTTTCCGGCCTCCTGC
CCGAGCACGAGCGCGATACTCTGGTCGAGGAGATGCTGCTGGCAAAGGAGGCCTGCAAGCCCTTTGATCGGCAAGCGTTC
CGTGAGGGGCATCTGACACCGGTCTATTTCGGCTCGGCGCTACGTAATTTTGGCGTCCGCGACCTGATAGAGGCGCTGGG
CGCCATCGGCCCTGCCCCGCGCGCCCAGGATGCGGACTCCCGCAAGGTGGAGGCGACCGAGGAGAAGATGACGTCATTCG
TCTTCAAGATTCAGGCCAATATGGATCCGAACCACCGGGATCGTATCGCCTTCGTGCGCGTGTGCTCCGGCAGGCTGGAG
CGCGGCATGAAGGCAAAGCTCGTGCGCACGGGAAAGCCCATCAGCCTTTCCGCACCGCAGTTCTTTTTCGCCAAGAACAG
GATCACCGCCGACGAGGCCTTCGCCGGCGACGTGGTGGGCATTCCCAACCACGGCACACTCAGGATTGGCGATACGCTGA
CGGAGGGGGAGGACATCCTCTTCCGCGGTGTGCCGAATTTCGCGCCCGAGATCCTGCGCCGCGTACGGCTCTCCGATGCA
ATGAAGGCGAAGAAGCTGAAAGAGGCCCTGCAGCAAATGGCCGAGGAAGGCGTGGTGCAGCTCTTCTCGCCCGAGGACGG
CTCACCGGCGATTGTCGGTGTCGTCGGCGCGCTGCAGCTCGACGTGCTCAAGGAGCGGCTGAAGAACGAATACACGCTGC
CGGTGGATTTCGAAATGGCGCGCTTCACGATCTGCCGCTGGATCTCGGCTGAAGACAAGGCTGAGCTCGAGCGTTTCATT
ACCGCGCATCGCGGCGACATTGCGCGGGATCTCGACGAGGACCCGGTGTTTCTGGCGCCACACGAATTCGGCCTCAAATA
CGAGGCGGAGCGCTGGAAGGCGATCACCTTCACCGCGGTCAAGGATTATCAGGTGAGGGAAAAGGCCAAAGCGGCGTAG

Upstream 100 bases:

>100_bases
GCGATGCTACAATTACGGCCGAGGCTTCCTACATATGGATTTCTGTTCGACAGCGGCGGTCATGACTGGTAAGAGGCCGC
CGTCAAAAGGATTTCCGGAT

Downstream 100 bases:

>100_bases
CTCATTCCGACTAGAGGCTGCTTTTGATCTTCTCGGCCAGCTGATGGAGCTCTTCCCGCCGGTAGGGCTCGCGCACGCCG
TGGCCCCAGACCGGACCCGG

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 532; Mature: 531

Protein sequence:

>532_residues
MPESLADAVARRRTFAIISHPDAGKTTLTEKLLLFGGAIQLAGEVKAKKNRIQTRSDWMAIERERGISVVTSVMTFEYGG
HVFNLLDTPGHEDFADDTYRTLTAVDSAVMVIDAAKGIEPRTLKLFEVCRLRDIPIITFVNKLDRESRDPFEILDEIEQK
LALDTAPITWPIGRGKSFSGTYHLANNTIRHSDDDVEPIRVNGPEAEAVSGLLPEHERDTLVEEMLLAKEACKPFDRQAF
REGHLTPVYFGSALRNFGVRDLIEALGAIGPAPRAQDADSRKVEATEEKMTSFVFKIQANMDPNHRDRIAFVRVCSGRLE
RGMKAKLVRTGKPISLSAPQFFFAKNRITADEAFAGDVVGIPNHGTLRIGDTLTEGEDILFRGVPNFAPEILRRVRLSDA
MKAKKLKEALQQMAEEGVVQLFSPEDGSPAIVGVVGALQLDVLKERLKNEYTLPVDFEMARFTICRWISAEDKAELERFI
TAHRGDIARDLDEDPVFLAPHEFGLKYEAERWKAITFTAVKDYQVREKAKAA

Sequences:

>Translated_532_residues
MPESLADAVARRRTFAIISHPDAGKTTLTEKLLLFGGAIQLAGEVKAKKNRIQTRSDWMAIERERGISVVTSVMTFEYGG
HVFNLLDTPGHEDFADDTYRTLTAVDSAVMVIDAAKGIEPRTLKLFEVCRLRDIPIITFVNKLDRESRDPFEILDEIEQK
LALDTAPITWPIGRGKSFSGTYHLANNTIRHSDDDVEPIRVNGPEAEAVSGLLPEHERDTLVEEMLLAKEACKPFDRQAF
REGHLTPVYFGSALRNFGVRDLIEALGAIGPAPRAQDADSRKVEATEEKMTSFVFKIQANMDPNHRDRIAFVRVCSGRLE
RGMKAKLVRTGKPISLSAPQFFFAKNRITADEAFAGDVVGIPNHGTLRIGDTLTEGEDILFRGVPNFAPEILRRVRLSDA
MKAKKLKEALQQMAEEGVVQLFSPEDGSPAIVGVVGALQLDVLKERLKNEYTLPVDFEMARFTICRWISAEDKAELERFI
TAHRGDIARDLDEDPVFLAPHEFGLKYEAERWKAITFTAVKDYQVREKAKAA
>Mature_531_residues
PESLADAVARRRTFAIISHPDAGKTTLTEKLLLFGGAIQLAGEVKAKKNRIQTRSDWMAIERERGISVVTSVMTFEYGGH
VFNLLDTPGHEDFADDTYRTLTAVDSAVMVIDAAKGIEPRTLKLFEVCRLRDIPIITFVNKLDRESRDPFEILDEIEQKL
ALDTAPITWPIGRGKSFSGTYHLANNTIRHSDDDVEPIRVNGPEAEAVSGLLPEHERDTLVEEMLLAKEACKPFDRQAFR
EGHLTPVYFGSALRNFGVRDLIEALGAIGPAPRAQDADSRKVEATEEKMTSFVFKIQANMDPNHRDRIAFVRVCSGRLER
GMKAKLVRTGKPISLSAPQFFFAKNRITADEAFAGDVVGIPNHGTLRIGDTLTEGEDILFRGVPNFAPEILRRVRLSDAM
KAKKLKEALQQMAEEGVVQLFSPEDGSPAIVGVVGALQLDVLKERLKNEYTLPVDFEMARFTICRWISAEDKAELERFIT
AHRGDIARDLDEDPVFLAPHEFGLKYEAERWKAITFTAVKDYQVREKAKAA

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=475, Percent_Identity=26.9473684210526, Blast_Score=148, Evalue=2e-35,
Organism=Homo sapiens, GI19923640, Length=500, Percent_Identity=26.8, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI25306283, Length=393, Percent_Identity=28.7531806615776, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI25306287, Length=478, Percent_Identity=26.7782426778243, Blast_Score=129, Evalue=7e-30,
Organism=Homo sapiens, GI157426893, Length=151, Percent_Identity=34.4370860927152, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI94966754, Length=147, Percent_Identity=30.6122448979592, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI4503483, Length=159, Percent_Identity=28.9308176100629, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1790835, Length=523, Percent_Identity=49.7131931166348, Blast_Score=524, Evalue=1e-150,
Organism=Escherichia coli, GI1789738, Length=487, Percent_Identity=26.4887063655031, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1788922, Length=157, Percent_Identity=33.7579617834395, Blast_Score=99, Evalue=1e-21,
Organism=Escherichia coli, GI48994988, Length=149, Percent_Identity=32.2147651006711, Blast_Score=85, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17533571, Length=487, Percent_Identity=26.4887063655031, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17556745, Length=486, Percent_Identity=24.2798353909465, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17557151, Length=132, Percent_Identity=34.0909090909091, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17506493, Length=170, Percent_Identity=27.0588235294118, Blast_Score=67, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6323098, Length=477, Percent_Identity=28.3018867924528, Blast_Score=175, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6322359, Length=534, Percent_Identity=23.4082397003745, Blast_Score=113, Evalue=9e-26,
Organism=Saccharomyces cerevisiae, GI6323320, Length=157, Percent_Identity=28.6624203821656, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324707, Length=159, Percent_Identity=31.4465408805031, Blast_Score=70, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6320593, Length=159, Percent_Identity=31.4465408805031, Blast_Score=70, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24582462, Length=481, Percent_Identity=26.6112266112266, Blast_Score=149, Evalue=4e-36,
Organism=Drosophila melanogaster, GI221458488, Length=507, Percent_Identity=26.6272189349112, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI78706572, Length=160, Percent_Identity=32.5, Blast_Score=100, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24585709, Length=161, Percent_Identity=29.1925465838509, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24585711, Length=153, Percent_Identity=29.4117647058824, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24585713, Length=153, Percent_Identity=29.4117647058824, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 59478; Mature: 59346

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPESLADAVARRRTFAIISHPDAGKTTLTEKLLLFGGAIQLAGEVKAKKNRIQTRSDWMA
CCHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHH
IERERGISVVTSVMTFEYGGHVFNLLDTPGHEDFADDTYRTLTAVDSAVMVIDAAKGIEP
HHHHCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHEEEEEECCCCCCC
RTLKLFEVCRLRDIPIITFVNKLDRESRDPFEILDEIEQKLALDTAPITWPIGRGKSFSG
HHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCCC
TYHLANNTIRHSDDDVEPIRVNGPEAEAVSGLLPEHERDTLVEEMLLAKEACKPFDRQAF
EEEECCCCCCCCCCCCCCEEECCCCHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHH
REGHLTPVYFGSALRNFGVRDLIEALGAIGPAPRAQDADSRKVEATEEKMTSFVFKIQAN
HCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHEEEEEECC
MDPNHRDRIAFVRVCSGRLERGMKAKLVRTGKPISLSAPQFFFAKNRITADEAFAGDVVG
CCCCCCHHHHHHHHHHHHHHCCCHHHHEECCCCCEECCCCCEEECCCCCCCHHCCCCEEE
IPNHGTLRIGDTLTEGEDILFRGVPNFAPEILRRVRLSDAMKAKKLKEALQQMAEEGVVQ
CCCCCEEEECCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LFSPEDGSPAIVGVVGALQLDVLKERLKNEYTLPVDFEMARFTICRWISAEDKAELERFI
EECCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHH
TAHRGDIARDLDEDPVFLAPHEFGLKYEAERWKAITFTAVKDYQVREKAKAA
HHHCCCHHHCCCCCCEEECCHHCCCCEECCCEEEEEEEEECCHHHHHHHCCC
>Mature Secondary Structure 
PESLADAVARRRTFAIISHPDAGKTTLTEKLLLFGGAIQLAGEVKAKKNRIQTRSDWMA
CHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHH
IERERGISVVTSVMTFEYGGHVFNLLDTPGHEDFADDTYRTLTAVDSAVMVIDAAKGIEP
HHHHCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHEEEEEECCCCCCC
RTLKLFEVCRLRDIPIITFVNKLDRESRDPFEILDEIEQKLALDTAPITWPIGRGKSFSG
HHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCCC
TYHLANNTIRHSDDDVEPIRVNGPEAEAVSGLLPEHERDTLVEEMLLAKEACKPFDRQAF
EEEECCCCCCCCCCCCCCEEECCCCHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHH
REGHLTPVYFGSALRNFGVRDLIEALGAIGPAPRAQDADSRKVEATEEKMTSFVFKIQAN
HCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHEEEEEECC
MDPNHRDRIAFVRVCSGRLERGMKAKLVRTGKPISLSAPQFFFAKNRITADEAFAGDVVG
CCCCCCHHHHHHHHHHHHHHCCCHHHHEECCCCCEECCCCCEEECCCCCCCHHCCCCEEE
IPNHGTLRIGDTLTEGEDILFRGVPNFAPEILRRVRLSDAMKAKKLKEALQQMAEEGVVQ
CCCCCEEEECCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LFSPEDGSPAIVGVVGALQLDVLKERLKNEYTLPVDFEMARFTICRWISAEDKAELERFI
EECCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHH
TAHRGDIARDLDEDPVFLAPHEFGLKYEAERWKAITFTAVKDYQVREKAKAA
HHHCCCHHHCCCCCCEEECCHHCCCCEECCCEEEEEEEEECCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA