Click here to switch to the map view.

The map label for this gene is fabI2 [H]

Identifier: 110636261

GI number: 110636261

Start: 4241277

End: 4242125

Strand: Reverse

Name: fabI2 [H]

Synonym: Meso_3937

Alternate gene names: 110636261

Gene position: 4242125-4241277 (Counterclockwise)

Preceding gene: 110636262

Following gene: 110636250

Centisome position: 96.14

GC content: 59.13

Gene sequence:

>849_bases
ATGGAATCTCTTATGAAGGGGAAGCGCGGCGTCATCATGGGCGTCGCGAACGATCATTCGATCGCCTGGGGCATTGCAAA
GAAACTCGCCGAGCAAGGGGCGCAGCTCGCATTTACCTATCAGGGGGATGCTTTCGGGCGCCGCGTGAAGCCACTGGCCG
ATCAGTTGGGAGCAAAGCTGCTTCTGCCCTGCGACGTCGAAGACAGCGCCTCGATTGATGGCGTTTTCGAGACCCTTGCG
TCGGAATGGGGCTCGCTCGATTTCCTCGTGCATGCCATCGGTTTTTCCGACCGCAACGAGCTGAAAGGCCTTTACGCCAA
CACCACGCGGGAAAACTTCATCCGCACGATGGTTATCTCCTGCTATTCCTTCACCGAAGTCAGCCGCAAGGCGGCGGCAA
TGATGGACAATGGCGGGAGCCTCCTCACCCTTACCTATGCCGGATCCGTCCGCGTGATGCCCAATTACAACGTGATGGGC
GTTGCCAAGGCCGGCCTTGAAGCGAGTGTGCGCTATCTTGCCAATGATTACGGCCCGCGCGGAATCCGCGTGAACGCGAT
TTCTGCCGGCCCGGTGCGGACGCTTGCAGGCGCTGGAATTTCCGACGCGCGGCATATGTTCACCTACCAGCAACGCAACT
CACCACTGCGCCGCACCGTCACGATAGACGAGATCGGCGGGTCAGCGCTCTATCTGCTGTCGGAACTTTCCTCGGGCGTA
ACGGGGGAAATCCACTATGTGGATTCGGGCTACAATATCGTTTCCATGCCGGTGCTTGAGGAACTGAAGCGCTCAGGCGA
AGCCTCCGAAAAGGCCCAAGCCGAGGGCGACGTCCCGAAAGCGAAATAG

Upstream 100 bases:

>100_bases
ATCGACAACGCAAAGATAGACACCGTTCTGTCGAACTCGTTCGGCTTCGGCGGCACCAATGCGACGCTGGTGTTTCAGCG
ATACGCGGGCTGAGGGGATG

Downstream 100 bases:

>100_bases
CGGGCGCAACCGTCCAACGGACAGCGGGCTATTTCCGTCCCCACAGCACCTTATAGCTTTCGTCGCGGGCGATTTCGCCC
TGCTGGCGGAAGGCGTTTTC

Product: enoyl-(acyl carrier protein) reductase

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase 2 [H]

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MESLMKGKRGVIMGVANDHSIAWGIAKKLAEQGAQLAFTYQGDAFGRRVKPLADQLGAKLLLPCDVEDSASIDGVFETLA
SEWGSLDFLVHAIGFSDRNELKGLYANTTRENFIRTMVISCYSFTEVSRKAAAMMDNGGSLLTLTYAGSVRVMPNYNVMG
VAKAGLEASVRYLANDYGPRGIRVNAISAGPVRTLAGAGISDARHMFTYQQRNSPLRRTVTIDEIGGSALYLLSELSSGV
TGEIHYVDSGYNIVSMPVLEELKRSGEASEKAQAEGDVPKAK

Sequences:

>Translated_282_residues
MESLMKGKRGVIMGVANDHSIAWGIAKKLAEQGAQLAFTYQGDAFGRRVKPLADQLGAKLLLPCDVEDSASIDGVFETLA
SEWGSLDFLVHAIGFSDRNELKGLYANTTRENFIRTMVISCYSFTEVSRKAAAMMDNGGSLLTLTYAGSVRVMPNYNVMG
VAKAGLEASVRYLANDYGPRGIRVNAISAGPVRTLAGAGISDARHMFTYQQRNSPLRRTVTIDEIGGSALYLLSELSSGV
TGEIHYVDSGYNIVSMPVLEELKRSGEASEKAQAEGDVPKAK
>Mature_282_residues
MESLMKGKRGVIMGVANDHSIAWGIAKKLAEQGAQLAFTYQGDAFGRRVKPLADQLGAKLLLPCDVEDSASIDGVFETLA
SEWGSLDFLVHAIGFSDRNELKGLYANTTRENFIRTMVISCYSFTEVSRKAAAMMDNGGSLLTLTYAGSVRVMPNYNVMG
VAKAGLEASVRYLANDYGPRGIRVNAISAGPVRTLAGAGISDARHMFTYQQRNSPLRRTVTIDEIGGSALYLLSELSSGV
TGEIHYVDSGYNIVSMPVLEELKRSGEASEKAQAEGDVPKAK

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787545, Length=258, Percent_Identity=50, Blast_Score=263, Evalue=9e-72,
Organism=Escherichia coli, GI1789378, Length=257, Percent_Identity=26.0700389105058, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI2367365, Length=249, Percent_Identity=29.718875502008, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI87082100, Length=259, Percent_Identity=25.0965250965251, Blast_Score=67, Evalue=9e-13,
Organism=Escherichia coli, GI1787335, Length=253, Percent_Identity=27.6679841897233, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 30357; Mature: 30357

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESLMKGKRGVIMGVANDHSIAWGIAKKLAEQGAQLAFTYQGDAFGRRVKPLADQLGAKL
CCCHHCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHCCCEE
LLPCDVEDSASIDGVFETLASEWGSLDFLVHAIGFSDRNELKGLYANTTRENFIRTMVIS
EEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCHHHCCCEECCCHHHHHHHHHHHH
CYSFTEVSRKAAAMMDNGGSLLTLTYAGSVRVMPNYNVMGVAKAGLEASVRYLANDYGPR
HHHHHHHHHHHHHHHCCCCCEEEEEECCEEEECCCCCEEEEECCCHHHHHHHHHHCCCCC
GIRVNAISAGPVRTLAGAGISDARHMFTYQQRNSPLRRTVTIDEIGGSALYLLSELSSGV
CEEEEEECCCCHHHHHCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHCCCC
TGEIHYVDSGYNIVSMPVLEELKRSGEASEKAQAEGDVPKAK
CEEEEEEECCCCEEEHHHHHHHHHCCCCCHHHHCCCCCCCCC
>Mature Secondary Structure
MESLMKGKRGVIMGVANDHSIAWGIAKKLAEQGAQLAFTYQGDAFGRRVKPLADQLGAKL
CCCHHCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHCCCEE
LLPCDVEDSASIDGVFETLASEWGSLDFLVHAIGFSDRNELKGLYANTTRENFIRTMVIS
EEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCHHHCCCEECCCHHHHHHHHHHHH
CYSFTEVSRKAAAMMDNGGSLLTLTYAGSVRVMPNYNVMGVAKAGLEASVRYLANDYGPR
HHHHHHHHHHHHHHHCCCCCEEEEEECCEEEECCCCCEEEEECCCHHHHHHHHHHCCCCC
GIRVNAISAGPVRTLAGAGISDARHMFTYQQRNSPLRRTVTIDEIGGSALYLLSELSSGV
CEEEEEECCCCHHHHHCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHCCCC
TGEIHYVDSGYNIVSMPVLEELKRSGEASEKAQAEGDVPKAK
CEEEEEEECCCCEEEHHHHHHHHHCCCCCHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]