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The map label for this gene is purD [H]

Identifier: 110633076

GI number: 110633076

Start: 794691

End: 795971

Strand: Direct

Name: purD [H]

Synonym: Meso_0719

Alternate gene names: 110633076

Gene position: 794691-795971 (Clockwise)

Preceding gene: 110633074

Following gene: 110633077

Centisome position: 18.01

GC content: 64.48

Gene sequence:

>1281_bases
ATGAAAGTTCTTCTCATCGGTTCGGGCGGGCGCGAGCATGCATTGGCGTGGAAGCTGGCGGCCTCGCCTCTTCTGGAGAA
GCTTTTTGCCGCGCCCGGCAATCCGGGAATTGCCGAACATGCCGAATGCGTGTCGCTCGACATCCGGGATCATGAGGCGG
TGATTGGTTTCTGCCGGGACCACGGGATCGGCTTCGTCGTCGTCGGTCCTGAGGCCCCTCTGGTGGCCGGCCTTGCCGAC
GACCTGATTGCCGCCGGGATCCCGGTCTTCGGCCCCACCGCGAGTGCTGCCCGTCTCGAAGGCTCCAAGGGTTTTACCAA
GGATCTCTGCGCGCGCTACGATATCCCGACCGCTGGCTATGCCCGCTTCCAAGATGCTGCGCACGCCAAAGATTATATCC
GCAAGGTGGGCGTGCCGATCGTCATCAAGGCCGATGGACTGGCGGCCGGAAAGGGCGTCACCATCGCCGTGACGGAGGAG
GAGGCTTTCGCAGCGGTGGACGATTGCTTCTCCGGCGTCTTCGGCGATTCGGGCGCGGAGGTGGTGGTGGAGGAATTGCT
TGTTGGCGAGGAAGCGAGCTTCTTCTGCCTGTGCGACGGCAAGACGGCGCTGCCCTTTGGCACCGCGCAGGACCACAAGC
GCGTGGGCGAGGGGGATACAGGCCCAAACACCGGCGGGATGGGCGCATATTCGCCGGCCCCCGTGATGACGTCCGACATG
GTTTCGCAGGTCATGCGCGAGATCATTGAGCCGACCTTGAAAGGTATGGAGGAGATGGGCTCGCCCTTTGTGGGCGTGCT
CTATGCCGGCCTCATGATCACGAAAGAGGGGCCGAAGCTCATCGAATACAATGTGCGCTTCGGCGATCCCGAGTGCCAGG
TCCTGATGCTTCTGCTCAAGGACGATCTGCTGGCCCTCATGAAGGGCGCGGTCGACGGCCAGCTTGCGCATATGTCCGTG
CGCTGGCGCGACGATGCGGCGCTGACCGTGGTCATGGCTGCGAATGGCTATCCCGGAACGCCGGAGAAAGGCTCCATTAT
CGGCGGGCTGAACGCCGCGGCCGCCGAGGGCGCGGAGATTTTCCACGCCGGGACGGCGCTGAAGGACGGCGCGCTCGTTG
CCAATGGCGGGCGGGTCCTGAACGTGACCGCTCTTGGCCGCACGGTGACGGAGGCAGGCGACAAGGCCTATCGCGCCGTG
GACCGGATCGACTGGCCGGAAGGTTTTTGCCGCCGCGACATCGGCTGGCGGGCGATCGAGCGGGAATCCCGGAACACGTA
A

Upstream 100 bases:

>100_bases
CGCGTCAAGCCAATCCTTCTTGATATGTCTCGCTGCGGCTTGACCTCCGGCCTTTTCGGCCATAGCCCCATCGCAGGAAA
TTTGAGGCACGAGCAGGTCC

Downstream 100 bases:

>100_bases
CGGCGTTCACGGCATCGCGATCCCGTATCTACGCCGTTTGGTCAAGTCGACAGGCTCTCACCTGTGTTGCAAGATTGTGG
GTTGATCTTGAAGGGACAAT

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MKVLLIGSGGREHALAWKLAASPLLEKLFAAPGNPGIAEHAECVSLDIRDHEAVIGFCRDHGIGFVVVGPEAPLVAGLAD
DLIAAGIPVFGPTASAARLEGSKGFTKDLCARYDIPTAGYARFQDAAHAKDYIRKVGVPIVIKADGLAAGKGVTIAVTEE
EAFAAVDDCFSGVFGDSGAEVVVEELLVGEEASFFCLCDGKTALPFGTAQDHKRVGEGDTGPNTGGMGAYSPAPVMTSDM
VSQVMREIIEPTLKGMEEMGSPFVGVLYAGLMITKEGPKLIEYNVRFGDPECQVLMLLLKDDLLALMKGAVDGQLAHMSV
RWRDDAALTVVMAANGYPGTPEKGSIIGGLNAAAAEGAEIFHAGTALKDGALVANGGRVLNVTALGRTVTEAGDKAYRAV
DRIDWPEGFCRRDIGWRAIERESRNT

Sequences:

>Translated_426_residues
MKVLLIGSGGREHALAWKLAASPLLEKLFAAPGNPGIAEHAECVSLDIRDHEAVIGFCRDHGIGFVVVGPEAPLVAGLAD
DLIAAGIPVFGPTASAARLEGSKGFTKDLCARYDIPTAGYARFQDAAHAKDYIRKVGVPIVIKADGLAAGKGVTIAVTEE
EAFAAVDDCFSGVFGDSGAEVVVEELLVGEEASFFCLCDGKTALPFGTAQDHKRVGEGDTGPNTGGMGAYSPAPVMTSDM
VSQVMREIIEPTLKGMEEMGSPFVGVLYAGLMITKEGPKLIEYNVRFGDPECQVLMLLLKDDLLALMKGAVDGQLAHMSV
RWRDDAALTVVMAANGYPGTPEKGSIIGGLNAAAAEGAEIFHAGTALKDGALVANGGRVLNVTALGRTVTEAGDKAYRAV
DRIDWPEGFCRRDIGWRAIERESRNT
>Mature_426_residues
MKVLLIGSGGREHALAWKLAASPLLEKLFAAPGNPGIAEHAECVSLDIRDHEAVIGFCRDHGIGFVVVGPEAPLVAGLAD
DLIAAGIPVFGPTASAARLEGSKGFTKDLCARYDIPTAGYARFQDAAHAKDYIRKVGVPIVIKADGLAAGKGVTIAVTEE
EAFAAVDDCFSGVFGDSGAEVVVEELLVGEEASFFCLCDGKTALPFGTAQDHKRVGEGDTGPNTGGMGAYSPAPVMTSDM
VSQVMREIIEPTLKGMEEMGSPFVGVLYAGLMITKEGPKLIEYNVRFGDPECQVLMLLLKDDLLALMKGAVDGQLAHMSV
RWRDDAALTVVMAANGYPGTPEKGSIIGGLNAAAAEGAEIFHAGTALKDGALVANGGRVLNVTALGRTVTEAGDKAYRAV
DRIDWPEGFCRRDIGWRAIERESRNT

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=423, Percent_Identity=49.1725768321513, Blast_Score=400, Evalue=1e-111,
Organism=Homo sapiens, GI209869995, Length=423, Percent_Identity=49.1725768321513, Blast_Score=400, Evalue=1e-111,
Organism=Homo sapiens, GI209869993, Length=423, Percent_Identity=49.1725768321513, Blast_Score=400, Evalue=1e-111,
Organism=Homo sapiens, GI28416899, Length=423, Percent_Identity=49.1725768321513, Blast_Score=400, Evalue=1e-111,
Organism=Escherichia coli, GI1790438, Length=429, Percent_Identity=55.9440559440559, Blast_Score=456, Evalue=1e-129,
Organism=Caenorhabditis elegans, GI17567511, Length=420, Percent_Identity=41.1904761904762, Blast_Score=296, Evalue=1e-80,
Organism=Saccharomyces cerevisiae, GI6321203, Length=443, Percent_Identity=39.9548532731377, Blast_Score=307, Evalue=2e-84,
Organism=Drosophila melanogaster, GI24582400, Length=436, Percent_Identity=43.8073394495413, Blast_Score=325, Evalue=5e-89,
Organism=Drosophila melanogaster, GI62471619, Length=413, Percent_Identity=44.7941888619855, Blast_Score=323, Evalue=2e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 44733; Mature: 44733

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLLIGSGGREHALAWKLAASPLLEKLFAAPGNPGIAEHAECVSLDIRDHEAVIGFCRD
CEEEEEECCCCCCCEEEHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCEEEEEEHH
HGIGFVVVGPEAPLVAGLADDLIAAGIPVFGPTASAARLEGSKGFTKDLCARYDIPTAGY
CCCEEEEECCCCCEEHHHHHHHHHCCCCCCCCCCCHHEECCCCCCHHHHHHHCCCCCCCH
ARFQDAAHAKDYIRKVGVPIVIKADGLAAGKGVTIAVTEEEAFAAVDDCFSGVFGDSGAE
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCHH
VVVEELLVGEEASFFCLCDGKTALPFGTAQDHKRVGEGDTGPNTGGMGAYSPAPVMTSDM
HHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHH
VSQVMREIIEPTLKGMEEMGSPFVGVLYAGLMITKEGPKLIEYNVRFGDPECQVLMLLLK
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHCEEEECCCCCEEEEEEEECCCHHHHHHHHHH
DDLLALMKGAVDGQLAHMSVRWRDDAALTVVMAANGYPGTPEKGSIIGGLNAAAAEGAEI
HHHHHHHHCCCCCEEEEEEEEEECCCEEEEEEEECCCCCCCCCCCEEECCCHHHCCCCCE
FHAGTALKDGALVANGGRVLNVTALGRTVTEAGDKAYRAVDRIDWPEGFCRRDIGWRAIE
EECCCCCCCCEEEECCCEEEEEEECCCHHHHCCHHHHHHHHCCCCCCHHHHCCCCCHHHH
RESRNT
HCCCCC
>Mature Secondary Structure
MKVLLIGSGGREHALAWKLAASPLLEKLFAAPGNPGIAEHAECVSLDIRDHEAVIGFCRD
CEEEEEECCCCCCCEEEHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCEEEEEEHH
HGIGFVVVGPEAPLVAGLADDLIAAGIPVFGPTASAARLEGSKGFTKDLCARYDIPTAGY
CCCEEEEECCCCCEEHHHHHHHHHCCCCCCCCCCCHHEECCCCCCHHHHHHHCCCCCCCH
ARFQDAAHAKDYIRKVGVPIVIKADGLAAGKGVTIAVTEEEAFAAVDDCFSGVFGDSGAE
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCHH
VVVEELLVGEEASFFCLCDGKTALPFGTAQDHKRVGEGDTGPNTGGMGAYSPAPVMTSDM
HHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHH
VSQVMREIIEPTLKGMEEMGSPFVGVLYAGLMITKEGPKLIEYNVRFGDPECQVLMLLLK
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHCEEEECCCCCEEEEEEEECCCHHHHHHHHHH
DDLLALMKGAVDGQLAHMSVRWRDDAALTVVMAANGYPGTPEKGSIIGGLNAAAAEGAEI
HHHHHHHHCCCCCEEEEEEEEEECCCEEEEEEEECCCCCCCCCCCEEECCCHHHCCCCCE
FHAGTALKDGALVANGGRVLNVTALGRTVTEAGDKAYRAVDRIDWPEGFCRRDIGWRAIE
EECCCCCCCCEEEECCCEEEEEEECCCHHHHCCHHHHHHHHCCCCCCHHHHCCCCCHHHH
RESRNT
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]