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The map label for this gene is vatD [H]

Identifier: 110632738

GI number: 110632738

Start: 420348

End: 420986

Strand: Direct

Name: vatD [H]

Synonym: Meso_0377

Alternate gene names: 110632738

Gene position: 420348-420986 (Clockwise)

Preceding gene: 110632737

Following gene: 110632739

Centisome position: 9.53

GC content: 61.35

Gene sequence:

>639_bases
ATGCATGGACCCAAACCCGAACTCAAGCACCCTATCCAGATGCATCCCCGCGTCGGCTTCCTCAAGCCGCTGGTCGATGC
GCCCAACGTCGAGATCGGCGAATACACCTACTATGACGACCCGGAAGGGCCGGACCAGTTCGTCGAGAAATGCGTGCTGC
ACCACTACGAATTCATCGGCGACAGGCTGATCATCGGCCGCTTCTGCGCCATAGCGGAAGGCGCGCGCTTCATTATGAAC
GGTGCCAACCACATGCTCTCTGGTTTCTCGACCTATCCCTTCAACATCTTCGGCCAAGGCTGGGAGGAAGGTTTTGATCA
GGAGAGCTGGCGCCGTGAGGTTCGTGGCGACACGGTGGTGGGCAACGACGTGTGGATCGGCTTCGACGCGGTCATCATGC
CGGGTGTGAAGATTGGGGACGGCGCTATCGTCGGTGGGCGGGCGGTTGTCACGCACGACGTTCCCGCCTATGCGGTCGTC
GCGGGGAATCCGGCCAAGGTGATGAAGATGCGTTTTGACCCGACGACGATAAGGCGCCTGCAGGCGATCGCCTGGTGGAA
TTGGCCGGCGGACAAGATCACGCGCAATCTCGATGCCCTTCGCGGCCACGACCTCAAGCGTCTGGAGGCGGCCGAGTGA

Upstream 100 bases:

>100_bases
CCGTATTCCTCGCACGTCGTCCGGGACAAGGTCCATCCCGGTGCTCCTTGGGGCGAGCGCGCAAACGCCGCTTTCCATCT
GGCGATCACGGAGAATTCCC

Downstream 100 bases:

>100_bases
CCACGGGCGCGGATGCAGCCACAGAGCGGCAAGACAAACCGGGCGGCAAGCTTTTTATCCGCCCGTGGATCTTCATCCGC
GGCGTGCCGGCGATGAAGTT

Product: hexapaptide repeat-containing transferase

Products: CoA; acetylmaltose [C]

Alternate protein names: Virginiamycin acetyltransferase D; Vat(D) [H]

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MHGPKPELKHPIQMHPRVGFLKPLVDAPNVEIGEYTYYDDPEGPDQFVEKCVLHHYEFIGDRLIIGRFCAIAEGARFIMN
GANHMLSGFSTYPFNIFGQGWEEGFDQESWRREVRGDTVVGNDVWIGFDAVIMPGVKIGDGAIVGGRAVVTHDVPAYAVV
AGNPAKVMKMRFDPTTIRRLQAIAWWNWPADKITRNLDALRGHDLKRLEAAE

Sequences:

>Translated_212_residues
MHGPKPELKHPIQMHPRVGFLKPLVDAPNVEIGEYTYYDDPEGPDQFVEKCVLHHYEFIGDRLIIGRFCAIAEGARFIMN
GANHMLSGFSTYPFNIFGQGWEEGFDQESWRREVRGDTVVGNDVWIGFDAVIMPGVKIGDGAIVGGRAVVTHDVPAYAVV
AGNPAKVMKMRFDPTTIRRLQAIAWWNWPADKITRNLDALRGHDLKRLEAAE
>Mature_212_residues
MHGPKPELKHPIQMHPRVGFLKPLVDAPNVEIGEYTYYDDPEGPDQFVEKCVLHHYEFIGDRLIIGRFCAIAEGARFIMN
GANHMLSGFSTYPFNIFGQGWEEGFDQESWRREVRGDTVVGNDVWIGFDAVIMPGVKIGDGAIVGGRAVVTHDVPAYAVV
AGNPAKVMKMRFDPTTIRRLQAIAWWNWPADKITRNLDALRGHDLKRLEAAE

Specific function: Inactivates the A compounds of streptogramin antibiotics by acetylation, thus providing resistance to these antibiotics [H]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1786664, Length=51, Percent_Identity=58.8235294117647, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001451
- InterPro:   IPR018357
- InterPro:   IPR011004 [H]

Pfam domain/function: PF00132 Hexapep [H]

EC number: 2.3.1.79 [C]

Molecular weight: Translated: 23844; Mature: 23844

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHGPKPELKHPIQMHPRVGFLKPLVDAPNVEIGEYTYYDDPEGPDQFVEKCVLHHYEFIG
CCCCCCCCCCCCCCCCCCCCHHHHCCCCCCEECCEEECCCCCCHHHHHHHHHHHHHHHHC
DRLIIGRFCAIAEGARFIMNGANHMLSGFSTYPFNIFGQGWEEGFDQESWRREVRGDTVV
CCHHHHHHHHHHHCHHHHHCCHHHHHCCCCCCCCEEECCCHHCCCCHHHHHHHHCCCEEE
GNDVWIGFDAVIMPGVKIGDGAIVGGRAVVTHDVPAYAVVAGNPAKVMKMRFDPTTIRRL
CCCEEEEEHHEEECCEEECCCEEECCEEEEEECCCEEEEEECCCHHEEEEECCHHHHHHH
QAIAWWNWPADKITRNLDALRGHDLKRLEAAE
HHHHCCCCCHHHHHHHHHHHCCCCCCHHCCCC
>Mature Secondary Structure
MHGPKPELKHPIQMHPRVGFLKPLVDAPNVEIGEYTYYDDPEGPDQFVEKCVLHHYEFIG
CCCCCCCCCCCCCCCCCCCCHHHHCCCCCCEECCEEECCCCCCHHHHHHHHHHHHHHHHC
DRLIIGRFCAIAEGARFIMNGANHMLSGFSTYPFNIFGQGWEEGFDQESWRREVRGDTVV
CCHHHHHHHHHHHCHHHHHCCHHHHHCCCCCCCCEEECCCHHCCCCHHHHHHHHCCCEEE
GNDVWIGFDAVIMPGVKIGDGAIVGGRAVVTHDVPAYAVVAGNPAKVMKMRFDPTTIRRL
CCCEEEEEHHEEECCEEECCCEEECCEEEEEECCCEEEEEECCCHHEEEEECCHHHHHHH
QAIAWWNWPADKITRNLDALRGHDLKRLEAAE
HHHHCCCCCHHHHHHHHHHHCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: 0.20 {glucose} 0.11 {maltose}

Km value (mM): 90 {maltose}} 62 {glucose}} 0.018 {acetyl-CoA}} 40 {triethanolamine}} [C]

Substrates: Acetyl-CoA; maltose [C]

Specific reaction: Acetyl-CoA + maltose = CoA + acetylmaltose [C]

General reaction: Acyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8257133 [H]