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The map label for this gene is icl [H]

Identifier: 110632672

GI number: 110632672

Start: 345245

End: 346534

Strand: Direct

Name: icl [H]

Synonym: Meso_0311

Alternate gene names: 110632672

Gene position: 345245-346534 (Clockwise)

Preceding gene: 110632670

Following gene: 110632673

Centisome position: 7.82

GC content: 63.02

Gene sequence:

>1290_bases
ATGACAGATTTTTACACTCTTGTCCCCTCAGCTCCCGCGAGCCGCTTCGATGGCATCGAGCGAACCTATTCGGCTGAGGA
CGTGCGTCGGCTGCGTGGTTCCGTGACGATCCGGCATACGCTTGCCGAGCAAGGTGCCAACCAGCTTTGGAAGCTGTTGC
ACGAAGAGAACTTCGTGAACGCGCTGGGTGCGCTTTCCGGCAACCAAGCGATGCAGATGGTGCGCGCAGGGCTGAAGGCG
ATTTATCTTTCCGGCTGGCAGGTGGCGGCGGATGCCAACACCGCCTCGGCGATGTACCCCGACCAGTCGCTCTACCCGGC
CAATGCCGGGCCGGAGCTTGCACGCCGCATCAACCGCACGCTTCAGCGAGCCGATCAGATCGAGGTGGCGGAAGGGAAGG
GGCTCTCGGTCGAGACCTGGTTCGCGCCCATCGTGGCCGACGCCGAGGCGGGCTTTGGCGGGCCGCTCAACGCGTTCGAG
ATCATGAAGGCATATATCGAGGCGGGTGCAGCCGGCGTCCATTTCGAGGATCAGCTTGCCTCGGAAAAGAAGTGCGGCCA
CCTCGGCGGAAAGGTGCTGATCCCCACCGCGGCTCATATCCGCAACTTGACCGCCGCGCGGCTCGCGGCGGATATCATGG
GCGTGCCTTCGCTTATCGTCTGCCGCACGGATGCGGAGGCTGCCAAGCTCATCACCTCCGATATAGACGAGCGCGACCAG
CCCTTTGTGGATTATGAGGCGGGGCGCACCGTCGAGGGCTTCTATCAGGTGAAGAACGGGCTAGAGCCCTGCATCGCGCG
CGCCGTGGCTTATGCACCCTACAGCGACCTCATCTGGTGCGAGACCTCGAAGCCGGATCTTGAGCAGGCGCGCAAATTCG
CCGAGGGCGTGCACAAGCACCATCCGGGCAAGATGCTGGCCTATAACTGCTCCCCGTCGTTTAACTGGAAGAAGAACCTG
GACGACGCGACCATCGCGAAATTCCAGCGCGAGTTGGGCGCCATGGGCTACAAATTCCAGTTCATCACACTCGCCGGCTT
CCACCAGCTCAATTACGGCATGTTCGAGCTGGCGCGCGGATACCGCGACCGGCAGATGGCCGCCTATGCCGAATTGCAGG
AGGCCGAGTTCGCCGCCGAGGCGCGCGGCTACACGGCCACGAAGCATCAGCGCGAGGTGGGCACGGGCTATTTCGATGCC
GTGTCCCTGGCGATCACGGGGGGCCAGTCCTCGACGACCGCCATGCACGGCTCCACCGAGCATGATCAATTCACTCAGGC
CGCCGAATAG

Upstream 100 bases:

>100_bases
GTCTTTCTAGCCCTTCTGACGTAAATCCCTGTCATAGCAAATGCCGATATGGCTCGTGCAGCCTTCTATTGCACGAATGC
AAACCTCTGATGGAGATACT

Downstream 100 bases:

>100_bases
CGGAAGGAATGGAGGCAGGCATGAAGATCTCTGTCAAGGAACGCGTCGAGGCCCAGGCGACTTCGATGGACGATGCCCAG
CAGGTGGCGATGCGCCGGGT

Product: isocitrate lyase

Products: NA

Alternate protein names: ICL; Isocitrase; Isocitratase [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MTDFYTLVPSAPASRFDGIERTYSAEDVRRLRGSVTIRHTLAEQGANQLWKLLHEENFVNALGALSGNQAMQMVRAGLKA
IYLSGWQVAADANTASAMYPDQSLYPANAGPELARRINRTLQRADQIEVAEGKGLSVETWFAPIVADAEAGFGGPLNAFE
IMKAYIEAGAAGVHFEDQLASEKKCGHLGGKVLIPTAAHIRNLTAARLAADIMGVPSLIVCRTDAEAAKLITSDIDERDQ
PFVDYEAGRTVEGFYQVKNGLEPCIARAVAYAPYSDLIWCETSKPDLEQARKFAEGVHKHHPGKMLAYNCSPSFNWKKNL
DDATIAKFQRELGAMGYKFQFITLAGFHQLNYGMFELARGYRDRQMAAYAELQEAEFAAEARGYTATKHQREVGTGYFDA
VSLAITGGQSSTTAMHGSTEHDQFTQAAE

Sequences:

>Translated_429_residues
MTDFYTLVPSAPASRFDGIERTYSAEDVRRLRGSVTIRHTLAEQGANQLWKLLHEENFVNALGALSGNQAMQMVRAGLKA
IYLSGWQVAADANTASAMYPDQSLYPANAGPELARRINRTLQRADQIEVAEGKGLSVETWFAPIVADAEAGFGGPLNAFE
IMKAYIEAGAAGVHFEDQLASEKKCGHLGGKVLIPTAAHIRNLTAARLAADIMGVPSLIVCRTDAEAAKLITSDIDERDQ
PFVDYEAGRTVEGFYQVKNGLEPCIARAVAYAPYSDLIWCETSKPDLEQARKFAEGVHKHHPGKMLAYNCSPSFNWKKNL
DDATIAKFQRELGAMGYKFQFITLAGFHQLNYGMFELARGYRDRQMAAYAELQEAEFAAEARGYTATKHQREVGTGYFDA
VSLAITGGQSSTTAMHGSTEHDQFTQAAE
>Mature_428_residues
TDFYTLVPSAPASRFDGIERTYSAEDVRRLRGSVTIRHTLAEQGANQLWKLLHEENFVNALGALSGNQAMQMVRAGLKAI
YLSGWQVAADANTASAMYPDQSLYPANAGPELARRINRTLQRADQIEVAEGKGLSVETWFAPIVADAEAGFGGPLNAFEI
MKAYIEAGAAGVHFEDQLASEKKCGHLGGKVLIPTAAHIRNLTAARLAADIMGVPSLIVCRTDAEAAKLITSDIDERDQP
FVDYEAGRTVEGFYQVKNGLEPCIARAVAYAPYSDLIWCETSKPDLEQARKFAEGVHKHHPGKMLAYNCSPSFNWKKNLD
DATIAKFQRELGAMGYKFQFITLAGFHQLNYGMFELARGYRDRQMAAYAELQEAEFAAEARGYTATKHQREVGTGYFDAV
SLAITGGQSSTTAMHGSTEHDQFTQAAE

Specific function: Catalyzes the formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle. May be involved in the assimilation of one-carbon compounds via the isocitrate lyase- positive serine pathway [H]

COG id: COG2224

COG function: function code C; Isocitrate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1790445, Length=419, Percent_Identity=61.5751789976134, Blast_Score=520, Evalue=1e-149,
Organism=Escherichia coli, GI1786525, Length=198, Percent_Identity=32.8282828282828, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17561814, Length=429, Percent_Identity=69.4638694638695, Blast_Score=631, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71982926, Length=429, Percent_Identity=69.4638694638695, Blast_Score=631, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6325263, Length=242, Percent_Identity=37.603305785124, Blast_Score=160, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6320908, Length=260, Percent_Identity=37.3076923076923, Blast_Score=159, Evalue=7e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006254
- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.1 [H]

Molecular weight: Translated: 46996; Mature: 46865

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDFYTLVPSAPASRFDGIERTYSAEDVRRLRGSVTIRHTLAEQGANQLWKLLHEENFVN
CCCCEEECCCCCHHHHCCHHHHCCHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHH
ALGALSGNQAMQMVRAGLKAIYLSGWQVAADANTASAMYPDQSLYPANAGPELARRINRT
HHHHCCCHHHHHHHHHHHHHEEECCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
LQRADQIEVAEGKGLSVETWFAPIVADAEAGFGGPLNAFEIMKAYIEAGAAGVHFEDQLA
HHHHCCEEECCCCCCEEEHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHH
SEKKCGHLGGKVLIPTAAHIRNLTAARLAADIMGVPSLIVCRTDAEAAKLITSDIDERDQ
HHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHCCCCCC
PFVDYEAGRTVEGFYQVKNGLEPCIARAVAYAPYSDLIWCETSKPDLEQARKFAEGVHKH
CCEECCCCCCHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHC
HPGKMLAYNCSPSFNWKKNLDDATIAKFQRELGAMGYKFQFITLAGFHQLNYGMFELARG
CCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEEECHHHHCCHHHHHHHC
YRDRQMAAYAELQEAEFAAEARGYTATKHQREVGTGYFDAVSLAITGGQSSTTAMHGSTE
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHEEEEEEEECCCCCCEEECCCCC
HDQFTQAAE
HHHHHHCCC
>Mature Secondary Structure 
TDFYTLVPSAPASRFDGIERTYSAEDVRRLRGSVTIRHTLAEQGANQLWKLLHEENFVN
CCCEEECCCCCHHHHCCHHHHCCHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHH
ALGALSGNQAMQMVRAGLKAIYLSGWQVAADANTASAMYPDQSLYPANAGPELARRINRT
HHHHCCCHHHHHHHHHHHHHEEECCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
LQRADQIEVAEGKGLSVETWFAPIVADAEAGFGGPLNAFEIMKAYIEAGAAGVHFEDQLA
HHHHCCEEECCCCCCEEEHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHH
SEKKCGHLGGKVLIPTAAHIRNLTAARLAADIMGVPSLIVCRTDAEAAKLITSDIDERDQ
HHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHCCCCCC
PFVDYEAGRTVEGFYQVKNGLEPCIARAVAYAPYSDLIWCETSKPDLEQARKFAEGVHKH
CCEECCCCCCHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHC
HPGKMLAYNCSPSFNWKKNLDDATIAKFQRELGAMGYKFQFITLAGFHQLNYGMFELARG
CCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEEECHHHHCCHHHHHHHC
YRDRQMAAYAELQEAEFAAEARGYTATKHQREVGTGYFDAVSLAITGGQSSTTAMHGSTE
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHEEEEEEEECCCCCCEEECCCCC
HDQFTQAAE
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]