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The map label for this gene is potF [H]

Identifier: 110632525

GI number: 110632525

Start: 199495

End: 200592

Strand: Direct

Name: potF [H]

Synonym: Meso_0163

Alternate gene names: 110632525

Gene position: 199495-200592 (Clockwise)

Preceding gene: 110632518

Following gene: 110632526

Centisome position: 4.52

GC content: 58.29

Gene sequence:

>1098_bases
ATGAACCGAAAGGCTGGTCTGTTTTCTGCCGTTTCCGTCGCTGCGGCGGTATTCGCCTCGGGCGCAGCGGCGCAGGAGCG
CACCGTCCACGTCTATAACTGGTCCGATTACATCGACGAATCGATCCTGCAGGAGTTCACTGCCGAGACCGGGATCAAAG
TCGTCTACGATCTCTTCGATTCCAATGAAATTCTGGAGACGAAGCTGCTTGCCGGTGGCACCGGCTATGACGTCGTTGTG
CCTTCGGGCGAGTTCTTGGCGCGCCAGATCCAAGCAGGCGTATTTCAGAAGCTCGACAAGTCGAAGCTGCCCAACCTCTC
GAACATGTGGGATTTCGTATCGGAGCGGACGGCCGTCTACGATCCGGGCAACGAGTATTCGGTCAACTATATGTGGGGCA
CCACCGGGATCGGCTATAACAAGGCGAAGGTGAAGGAGGCGCTCGGCACGGACAGGATCAATTCGTGGGACGTGGTCTTC
AAACCCGAGGTCGCTTCCAAGCTTGCCGGCTGCGGCATACACGTGCTCGATGCTTCGTCCGAGATCACACATGCTGCGCT
GAATTACCTCGGGCTCGACCCCAACAGCGACAATCCCGACGATCTTCAGAAGGTTGAGGCGCTGTTGCTTTCCGTGAGGC
CCAATATCCGCAAGTTCCACTCTTCCGAATATATCAACGCGCTTGCCAACGGAGACATCTGCGTGGCTGTCGGCTATTCG
GGCGACGTTTTCCAGGCCCGCGACCGGGCGGCGGAAGCCGGCCAGGGCGTGGAGATCGCCTATGCGATCCCGAAGGAAGG
CGCCCAGATGTGGTTCGACCAGATGGCGATCCCCGCTGATGCGCCTCATGTGGAGGAGGCTCACGAGTTTATCAACTACA
TGATGCGCCCCGAGGTGATCGCCAAGGCATCGAATTATGTATTCTACGCGAACGGCAATAAGGCTTCGCAGGAATTGCTC
GATGCGGAAGTTATCGATGATCCGGCGGTCTATCCCGACGACGCAACCCTGCAAAAACTCTTCACGGCCACGCCCTATGA
CTCGCGTGCGCAGCGCCTGATCACCCGCATGTGGACCAAGGTGGTCACCGGTCAGTAG

Upstream 100 bases:

>100_bases
CCTTGTCGCCCCCATCAAACGACAATAGGTTTGCCGCTGATTTCCCCGTTTTCCCGCGACGGAAAGGCGGGGCGGAACTG
GTCGACAATGGGAGACGAAA

Downstream 100 bases:

>100_bases
CGGCTATAAAGGTCCGGCGGGCTCGCCGGGCTCTCCGCCTCGGGGTGGGACAAGACGGAGCAGGCGATGGCATCGCTGGG
CAGCATTCGCAGGAGCTTTG

Product: extracellular solute-binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MNRKAGLFSAVSVAAAVFASGAAAQERTVHVYNWSDYIDESILQEFTAETGIKVVYDLFDSNEILETKLLAGGTGYDVVV
PSGEFLARQIQAGVFQKLDKSKLPNLSNMWDFVSERTAVYDPGNEYSVNYMWGTTGIGYNKAKVKEALGTDRINSWDVVF
KPEVASKLAGCGIHVLDASSEITHAALNYLGLDPNSDNPDDLQKVEALLLSVRPNIRKFHSSEYINALANGDICVAVGYS
GDVFQARDRAAEAGQGVEIAYAIPKEGAQMWFDQMAIPADAPHVEEAHEFINYMMRPEVIAKASNYVFYANGNKASQELL
DAEVIDDPAVYPDDATLQKLFTATPYDSRAQRLITRMWTKVVTGQ

Sequences:

>Translated_365_residues
MNRKAGLFSAVSVAAAVFASGAAAQERTVHVYNWSDYIDESILQEFTAETGIKVVYDLFDSNEILETKLLAGGTGYDVVV
PSGEFLARQIQAGVFQKLDKSKLPNLSNMWDFVSERTAVYDPGNEYSVNYMWGTTGIGYNKAKVKEALGTDRINSWDVVF
KPEVASKLAGCGIHVLDASSEITHAALNYLGLDPNSDNPDDLQKVEALLLSVRPNIRKFHSSEYINALANGDICVAVGYS
GDVFQARDRAAEAGQGVEIAYAIPKEGAQMWFDQMAIPADAPHVEEAHEFINYMMRPEVIAKASNYVFYANGNKASQELL
DAEVIDDPAVYPDDATLQKLFTATPYDSRAQRLITRMWTKVVTGQ
>Mature_365_residues
MNRKAGLFSAVSVAAAVFASGAAAQERTVHVYNWSDYIDESILQEFTAETGIKVVYDLFDSNEILETKLLAGGTGYDVVV
PSGEFLARQIQAGVFQKLDKSKLPNLSNMWDFVSERTAVYDPGNEYSVNYMWGTTGIGYNKAKVKEALGTDRINSWDVVF
KPEVASKLAGCGIHVLDASSEITHAALNYLGLDPNSDNPDDLQKVEALLLSVRPNIRKFHSSEYINALANGDICVAVGYS
GDVFQARDRAAEAGQGVEIAYAIPKEGAQMWFDQMAIPADAPHVEEAHEFINYMMRPEVIAKASNYVFYANGNKASQELL
DAEVIDDPAVYPDDATLQKLFTATPYDSRAQRLITRMWTKVVTGQ

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=363, Percent_Identity=53.168044077135, Blast_Score=391, Evalue=1e-110,
Organism=Escherichia coli, GI1787367, Length=323, Percent_Identity=34.0557275541796, Blast_Score=196, Evalue=3e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40122; Mature: 40122

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRKAGLFSAVSVAAAVFASGAAAQERTVHVYNWSDYIDESILQEFTAETGIKVVYDLFD
CCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHHHHHHHHHHCCCEEEEEHHC
SNEILETKLLAGGTGYDVVVPSGEFLARQIQAGVFQKLDKSKLPNLSNMWDFVSERTAVY
CCHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEE
DPGNEYSVNYMWGTTGIGYNKAKVKEALGTDRINSWDVVFKPEVASKLAGCGIHVLDASS
CCCCCEEEEEEEECCCCCCCHHHHHHHHCCCCCCCCCEEECCHHHHHHHCCCEEEECCCC
EITHAALNYLGLDPNSDNPDDLQKVEALLLSVRPNIRKFHSSEYINALANGDICVAVGYS
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEECCC
GDVFQARDRAAEAGQGVEIAYAIPKEGAQMWFDQMAIPADAPHVEEAHEFINYMMRPEVI
CCHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHH
AKASNYVFYANGNKASQELLDAEVIDDPAVYPDDATLQKLFTATPYDSRAQRLITRMWTK
HHCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHH
VVTGQ
HHCCC
>Mature Secondary Structure
MNRKAGLFSAVSVAAAVFASGAAAQERTVHVYNWSDYIDESILQEFTAETGIKVVYDLFD
CCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHHHHHHHHHHCCCEEEEEHHC
SNEILETKLLAGGTGYDVVVPSGEFLARQIQAGVFQKLDKSKLPNLSNMWDFVSERTAVY
CCHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEE
DPGNEYSVNYMWGTTGIGYNKAKVKEALGTDRINSWDVVFKPEVASKLAGCGIHVLDASS
CCCCCEEEEEEEECCCCCCCHHHHHHHHCCCCCCCCCEEECCHHHHHHHCCCEEEECCCC
EITHAALNYLGLDPNSDNPDDLQKVEALLLSVRPNIRKFHSSEYINALANGDICVAVGYS
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEECCC
GDVFQARDRAAEAGQGVEIAYAIPKEGAQMWFDQMAIPADAPHVEEAHEFINYMMRPEVI
CCHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHH
AKASNYVFYANGNKASQELLDAEVIDDPAVYPDDATLQKLFTATPYDSRAQRLITRMWTK
HHCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHH
VVTGQ
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]