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The map label for this gene is waaE

Identifier: 109947746

GI number: 109947746

Start: 1056884

End: 1058275

Strand: Reverse

Name: waaE

Synonym: Hac_1221

Alternate gene names: 109947746

Gene position: 1058275-1056884 (Counterclockwise)

Preceding gene: 109947747

Following gene: 109947745

Centisome position: 68.1

GC content: 39.73

Gene sequence:

>1392_bases
ATGAGAAAAATCTTAGTCATAGGGGATTTGATCGCTGATTATTATTTATGGGGGAAGAGCGAACGCCTTTCGCCTGAAGC
CCCTGTGCCTATTTTAGAAGTCAAAAAAGAGAGCAAGAGTTTAGGCGGAGCGGCTAATGTGGCCAATAACCTCATCTCTT
TAGGGGCTCAAGTCTTTTTATGTGGGGTAGTGGGCGATGATCTAGAGGGCAAGCATTTCATTAACGCTTTAAAAATAAGA
GAGGTTGACACTTCAGGCGTTTTAATAGATAAAACCCGTTGCACCACACTCAAAACGCGCATTATCGTGCAAAACCAGCA
AATCGTGCGCGTGGATAAGGAAATCAAAGATCCCTTAAACGCCGATTTAAGAAAGAACCTTTTAGACTTTGTCGCAGAAA
AAATCCAAGAAATGGATGGGATTATTCTTTCAGATTACAATAAGGGCGTTTTAGATTTTAAGATCACTCAAACTATCATC
GCATTAGCCAACAAACACTACAAGCTCATTTTATGCGACCCTAAAGGGAAAGATTATAGCAAATATTCCCATGCGAGTTT
GATCACGCCTAATCGTTTTGAATTGGAGCAAGCGCTTCATTTGAAATTAGACGGCGATGCCAATTTATTGAAAGCGCTTC
AAATCTTACAAGAAACTTACCATATTGCCATGCCTTTAGTTACTTTGAGCGAGCAAGGCATCGCTTTTTTAGAAAAAAAC
GAGCTAATCAATTGCCCCACCATCGCTAAAGAAGTTTATGATGTAACTGGGGCTGGCGATACAGTGATCGCATCTTTAAC
GCTCTCTTTATTGGAATCAAAAAATCTTAAAGAAGCTTGTGAGTTTGCCAATGCAGCAGCAGCTGTGGTGGTGGGTAAAA
TGGGGAGTGCGTTAGCGAGTTTGGAAGAAATCGCTTTGATTTTAAACCAAACGCACCCTAAAATCCTTTCTTTAGAAAAG
CTTTTAAACATTTTAGAGTATAATAAGCAAAAAGTCGTTTTCACCAATGGTTGTTTTGATATTCTCCATAAAGGGCATGC
GAGTTATTTGCAAAAGGCTAAAGCTTTAGGGGATATTCTCATTGTAGGGTTAAATAGCGATGCTTCCATTAAAAGGCTTA
AGGGGGATAAACGCCCCATAGTGGGCGAAAAAGACAGAGCGTTTCTTTTAGCGAGTTTGTCTTGCGTGGATTATATCGTG
GTGTTTGAAGAAGACACGCCCATAGGATTGATTCAAGCCCTAAAGCCTGATATTTTAGTTAAGGGGGCGGACTATCTTGA
TAAAGAAGTCATAGGGAGCGAGTTTGCTAAAGAAACTTGTTTGATGGCATTTGAAGAAGGGTATTCTACAAGTGCTATTA
TAGAAAAAATTAAAAGGGCATACAATGATTAA

Upstream 100 bases:

>100_bases
CTACTATTTTGGATTTAGATTACACCCCCTTATACGATTTAGAAAGCGGTATTAAGGATTATTTACCCCATATCTATACA
ATTTTTGAAGGAGGGAACGC

Downstream 100 bases:

>100_bases
TGATTTGATTAAAAAAGAATTTTTAGCCCATAAGGAAGCGTTAGAAAAAAGTTTAGAAAGTTTGGAAGAATCTTTGATCC
AAAGCGTCCAACTTTTGATA

Product: ADP-heptose synthase

Products: NA

Alternate protein names: D-beta-D-heptose 7-phosphate kinase; D-beta-D-heptose 7-phosphotransferase; D-beta-D-heptose 1-phosphate adenosyltransferase

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MRKILVIGDLIADYYLWGKSERLSPEAPVPILEVKKESKSLGGAANVANNLISLGAQVFLCGVVGDDLEGKHFINALKIR
EVDTSGVLIDKTRCTTLKTRIIVQNQQIVRVDKEIKDPLNADLRKNLLDFVAEKIQEMDGIILSDYNKGVLDFKITQTII
ALANKHYKLILCDPKGKDYSKYSHASLITPNRFELEQALHLKLDGDANLLKALQILQETYHIAMPLVTLSEQGIAFLEKN
ELINCPTIAKEVYDVTGAGDTVIASLTLSLLESKNLKEACEFANAAAAVVVGKMGSALASLEEIALILNQTHPKILSLEK
LLNILEYNKQKVVFTNGCFDILHKGHASYLQKAKALGDILIVGLNSDASIKRLKGDKRPIVGEKDRAFLLASLSCVDYIV
VFEEDTPIGLIQALKPDILVKGADYLDKEVIGSEFAKETCLMAFEEGYSTSAIIEKIKRAYND

Sequences:

>Translated_463_residues
MRKILVIGDLIADYYLWGKSERLSPEAPVPILEVKKESKSLGGAANVANNLISLGAQVFLCGVVGDDLEGKHFINALKIR
EVDTSGVLIDKTRCTTLKTRIIVQNQQIVRVDKEIKDPLNADLRKNLLDFVAEKIQEMDGIILSDYNKGVLDFKITQTII
ALANKHYKLILCDPKGKDYSKYSHASLITPNRFELEQALHLKLDGDANLLKALQILQETYHIAMPLVTLSEQGIAFLEKN
ELINCPTIAKEVYDVTGAGDTVIASLTLSLLESKNLKEACEFANAAAAVVVGKMGSALASLEEIALILNQTHPKILSLEK
LLNILEYNKQKVVFTNGCFDILHKGHASYLQKAKALGDILIVGLNSDASIKRLKGDKRPIVGEKDRAFLLASLSCVDYIV
VFEEDTPIGLIQALKPDILVKGADYLDKEVIGSEFAKETCLMAFEEGYSTSAIIEKIKRAYND
>Mature_463_residues
MRKILVIGDLIADYYLWGKSERLSPEAPVPILEVKKESKSLGGAANVANNLISLGAQVFLCGVVGDDLEGKHFINALKIR
EVDTSGVLIDKTRCTTLKTRIIVQNQQIVRVDKEIKDPLNADLRKNLLDFVAEKIQEMDGIILSDYNKGVLDFKITQTII
ALANKHYKLILCDPKGKDYSKYSHASLITPNRFELEQALHLKLDGDANLLKALQILQETYHIAMPLVTLSEQGIAFLEKN
ELINCPTIAKEVYDVTGAGDTVIASLTLSLLESKNLKEACEFANAAAAVVVGKMGSALASLEEIALILNQTHPKILSLEK
LLNILEYNKQKVVFTNGCFDILHKGHASYLQKAKALGDILIVGLNSDASIKRLKGDKRPIVGEKDRAFLLASLSCVDYIV
VFEEDTPIGLIQALKPDILVKGADYLDKEVIGSEFAKETCLMAFEEGYSTSAIIEKIKRAYND

Specific function: Catalyzes the ADP transfer to D-glycero-D-manno-heptose 1-phosphate, yielding ADP-D,D-heptose

COG id: COG2870

COG function: function code M; ADP-heptose synthase, bifunctional sugar kinase/adenylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the cytidylyltransferase family

Homologues:

Organism=Escherichia coli, GI1789432, Length=471, Percent_Identity=42.0382165605096, Blast_Score=321, Evalue=7e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HLDE_HELAH (Q17WK1)

Other databases:

- EMBL:   AM260522
- RefSeq:   YP_664974.1
- ProteinModelPortal:   Q17WK1
- SMR:   Q17WK1
- STRING:   Q17WK1
- GeneID:   4176636
- GenomeReviews:   AM260522_GR
- KEGG:   hac:Hac_1221
- NMPDR:   fig|382638.8.peg.1186
- eggNOG:   COG2870
- HOGENOM:   HBG545535
- OMA:   LTPNRKE
- PhylomeDB:   Q17WK1
- ProtClustDB:   CLSK496243
- BioCyc:   HACI382638:HAC_1221-MONOMER
- HAMAP:   MF_01603
- InterPro:   IPR023030
- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR004821
- InterPro:   IPR004820
- InterPro:   IPR011913
- InterPro:   IPR011914
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR00125
- TIGRFAMs:   TIGR02198
- TIGRFAMs:   TIGR02199

Pfam domain/function: PF01467 CTP_transf_2; PF00294 PfkB

EC number: 2.7.-.-

Molecular weight: Translated: 51013; Mature: 51013

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS00583 PFKB_KINASES_1; PS00584 PFKB_KINASES_2

Important sites: ACT_SITE 260-260

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKILVIGDLIADYYLWGKSERLSPEAPVPILEVKKESKSLGGAANVANNLISLGAQVFL
CCEEEEEHHHHHHHHHCCCCCCCCCCCCCCHHHHCHHHHHCCCHHHHHHHHHHHCHHEEE
CGVVGDDLEGKHFINALKIREVDTSGVLIDKTRCTTLKTRIIVQNQQIVRVDKEIKDPLN
EEECCCCCCCHHHHHHEEEEEECCCCEEEECCCCCEEEEEEEECCCHHEECCHHHCCCCC
ADLRKNLLDFVAEKIQEMDGIILSDYNKGVLDFKITQTIIALANKHYKLILCDPKGKDYS
HHHHHHHHHHHHHHHHHHCCEEEECCCCCEEEEHHHHHHHHHHCCCEEEEEECCCCCCCC
KYSHASLITPNRFELEQALHLKLDGDANLLKALQILQETYHIAMPLVTLSEQGIAFLEKN
CCCCCCCCCCCHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEECC
ELINCPTIAKEVYDVTGAGDTVIASLTLSLLESKNLKEACEFANAAAAVVVGKMGSALAS
CCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LEEIALILNQTHPKILSLEKLLNILEYNKQKVVFTNGCFDILHKGHASYLQKAKALGDIL
HHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEECCHHHHHHCCHHHHHHHHHHCCCEE
IVGLNSDASIKRLKGDKRPIVGEKDRAFLLASLSCVDYIVVFEEDTPIGLIQALKPDILV
EEEECCCCHHHHHCCCCCCCCCCCCCCCEEEECCCEEEEEEEECCCCHHHHHHCCCCEEE
KGADYLDKEVIGSEFAKETCLMAFEEGYSTSAIIEKIKRAYND
ECCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MRKILVIGDLIADYYLWGKSERLSPEAPVPILEVKKESKSLGGAANVANNLISLGAQVFL
CCEEEEEHHHHHHHHHCCCCCCCCCCCCCCHHHHCHHHHHCCCHHHHHHHHHHHCHHEEE
CGVVGDDLEGKHFINALKIREVDTSGVLIDKTRCTTLKTRIIVQNQQIVRVDKEIKDPLN
EEECCCCCCCHHHHHHEEEEEECCCCEEEECCCCCEEEEEEEECCCHHEECCHHHCCCCC
ADLRKNLLDFVAEKIQEMDGIILSDYNKGVLDFKITQTIIALANKHYKLILCDPKGKDYS
HHHHHHHHHHHHHHHHHHCCEEEECCCCCEEEEHHHHHHHHHHCCCEEEEEECCCCCCCC
KYSHASLITPNRFELEQALHLKLDGDANLLKALQILQETYHIAMPLVTLSEQGIAFLEKN
CCCCCCCCCCCHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEECC
ELINCPTIAKEVYDVTGAGDTVIASLTLSLLESKNLKEACEFANAAAAVVVGKMGSALAS
CCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LEEIALILNQTHPKILSLEKLLNILEYNKQKVVFTNGCFDILHKGHASYLQKAKALGDIL
HHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEECCHHHHHHCCHHHHHHHHHHCCCEE
IVGLNSDASIKRLKGDKRPIVGEKDRAFLLASLSCVDYIVVFEEDTPIGLIQALKPDILV
EEEECCCCHHHHHCCCCCCCCCCCCCCCEEEECCCEEEEEEEECCCCHHHHHHCCCCEEE
KGADYLDKEVIGSEFAKETCLMAFEEGYSTSAIIEKIKRAYND
ECCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA