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The map label for this gene is cusR [H]

Identifier: 109947684

GI number: 109947684

Start: 999589

End: 1000260

Strand: Reverse

Name: cusR [H]

Synonym: Hac_1154

Alternate gene names: 109947684

Gene position: 1000260-999589 (Counterclockwise)

Preceding gene: 109947685

Following gene: 109947683

Centisome position: 64.37

GC content: 42.41

Gene sequence:

>672_bases
ATGCGTGTTCTACTGATTGAAAAAGATTCTGTTTTAGGCGAAGAGATTGAGAAGGGCTTGAACGACAAAGGCTTTATGGC
TGATGTGACTGAAAGCGTAGAGGATGGGGAATATCTTATGGATATTAGGAATTATGACCTAGTGATGGTGAGCGATAAAA
ACGCTATTAATTTGGTTTCTAGAGTCAAAGACAAGCACCCCTCTATTATCGTTTTAGTTTCTTCTAGTAACCCCACAAGC
CAAGAAGAAGTCCATGCGTTTGAGCAGGGTGCAGACGATTATATCGCCAAACCTTATCGCAGCATTAAGGCTTTAGTCGC
AAGGATTGAGGCACGTTTGAGGTTTTGGGGATCTAATGTGATTGAAATTGGGGATTTGACCATCAGCCCTGATGAAGAAA
AGATTATTTACAAGGGGCGTGAGATTGAGGTTAAGGGCAAGCCTTTTGAAGTGCTCACACACCTTGCGAGGCATAGGGAT
CAAATCGTTTCAAAAGAACAGCTTTTAGACGCTATTTGGGAAGAGCCTGAAATGGTTACCCCTAATGTGATTGAAGTAGC
GATCAATCAAATCCGCCAAAAAATGGATAAGCCTTTAGGGATTTCCACGGTTGAAACGATAAGGCGCAGAGGCTATCGTT
TTTGTTACCCAAAGCCTGCGTGTGAAGAATAA

Upstream 100 bases:

>100_bases
CAAGTCAAGCGATAGCTTTTATTAAAATTTTCTTAAACTAACTTAAATTTGTGATATAATCAAGCTTTAATTACTCTTAA
ATATGGGAGCCAACAAAACC

Downstream 100 bases:

>100_bases
AGCCCTATTTTTGCGGACTTTTTCGCACTAGAACTAAATTTTAGCATGATAGCGATCGTTAATAGTGGTTTTGTGGTCAG
CTATCTCTTTTGAAGTAACC

Product: two-component system regulatory protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MRVLLIEKDSVLGEEIEKGLNDKGFMADVTESVEDGEYLMDIRNYDLVMVSDKNAINLVSRVKDKHPSIIVLVSSSNPTS
QEEVHAFEQGADDYIAKPYRSIKALVARIEARLRFWGSNVIEIGDLTISPDEEKIIYKGREIEVKGKPFEVLTHLARHRD
QIVSKEQLLDAIWEEPEMVTPNVIEVAINQIRQKMDKPLGISTVETIRRRGYRFCYPKPACEE

Sequences:

>Translated_223_residues
MRVLLIEKDSVLGEEIEKGLNDKGFMADVTESVEDGEYLMDIRNYDLVMVSDKNAINLVSRVKDKHPSIIVLVSSSNPTS
QEEVHAFEQGADDYIAKPYRSIKALVARIEARLRFWGSNVIEIGDLTISPDEEKIIYKGREIEVKGKPFEVLTHLARHRD
QIVSKEQLLDAIWEEPEMVTPNVIEVAINQIRQKMDKPLGISTVETIRRRGYRFCYPKPACEE
>Mature_223_residues
MRVLLIEKDSVLGEEIEKGLNDKGFMADVTESVEDGEYLMDIRNYDLVMVSDKNAINLVSRVKDKHPSIIVLVSSSNPTS
QEEVHAFEQGADDYIAKPYRSIKALVARIEARLRFWGSNVIEIGDLTISPDEEKIIYKGREIEVKGKPFEVLTHLARHRD
QIVSKEQLLDAIWEEPEMVTPNVIEVAINQIRQKMDKPLGISTVETIRRRGYRFCYPKPACEE

Specific function: Member of the two-component regulatory system CusS/CusR. Activates the expression of cusCFBA in response to increasing levels of copper ions, and probably silver ions [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786784, Length=225, Percent_Identity=27.1111111111111, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1787375, Length=221, Percent_Identity=23.9819004524887, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1789402, Length=219, Percent_Identity=27.8538812785388, Blast_Score=85, Evalue=3e-18,
Organism=Escherichia coli, GI87082012, Length=224, Percent_Identity=25.8928571428571, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790552, Length=219, Percent_Identity=25.1141552511416, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI2367329, Length=226, Percent_Identity=27.8761061946903, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1786599, Length=224, Percent_Identity=26.7857142857143, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1790860, Length=222, Percent_Identity=25.6756756756757, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1786911, Length=228, Percent_Identity=23.6842105263158, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR006291
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 25502; Mature: 25502

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLLIEKDSVLGEEIEKGLNDKGFMADVTESVEDGEYLMDIRNYDLVMVSDKNAINLVS
CEEEEEECCCHHHHHHHCCCCCCCCHHHHHHHHCCCCEEEEECCCCEEEEECCCHHHHHH
RVKDKHPSIIVLVSSSNPTSQEEVHAFEQGADDYIAKPYRSIKALVARIEARLRFWGSNV
HHHCCCCCEEEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCE
IEIGDLTISPDEEKIIYKGREIEVKGKPFEVLTHLARHRDQIVSKEQLLDAIWEEPEMVT
EEECCEEECCCCCEEEECCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PNVIEVAINQIRQKMDKPLGISTVETIRRRGYRFCYPKPACEE
CHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEECCCCCCCCC
>Mature Secondary Structure
MRVLLIEKDSVLGEEIEKGLNDKGFMADVTESVEDGEYLMDIRNYDLVMVSDKNAINLVS
CEEEEEECCCHHHHHHHCCCCCCCCHHHHHHHHCCCCEEEEECCCCEEEEECCCHHHHHH
RVKDKHPSIIVLVSSSNPTSQEEVHAFEQGADDYIAKPYRSIKALVARIEARLRFWGSNV
HHHCCCCCEEEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCE
IEIGDLTISPDEEKIIYKGREIEVKGKPFEVLTHLARHRDQIVSKEQLLDAIWEEPEMVT
EEECCEEECCCCCEEEECCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PNVIEVAINQIRQKMDKPLGISTVETIRRRGYRFCYPKPACEE
CHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]