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The map label for this gene is folE

Identifier: 109947622

GI number: 109947622

Start: 941850

End: 942392

Strand: Reverse

Name: folE

Synonym: Hac_1087

Alternate gene names: 109947622

Gene position: 942392-941850 (Counterclockwise)

Preceding gene: 109947623

Following gene: 109947621

Centisome position: 60.65

GC content: 35.54

Gene sequence:

>543_bases
ATGGAAAATTTTTTCAACCAATTTTTTGAAAACATCGGCGAAGATAAGAATAGAGAAGGTTTGAAAAACACGCCTAAAAG
AGTTCAAGAATTATGGAATTTCTTGTATAAAGGCTATAAAGAAGACCCTAAAGTGGCTTTAAAAAGTGCGTATTTTCAAG
GCGTTTGCGATGAAATGATAGTGGCTCAACACATTGAATTTTACTCCACTTGCGAGCATCATTTACTCCCTTTTTTTGGG
AATATTAGCTTAGGTTATATTCCTAAAGAAAAGATTATCGGCATTAGTGCGATCGCTAAACTCATTGAAATTTATAGCAA
ACGCTTACAAATCCAAGAAAGATTGACCACTCAAATTGCAGAAACCTTTGATGAAATCATAGAGCCAAGGGGCGTGATAG
TGATTTGTGAGGCCAAACATTTGTGCATGAGCATGCAAGGGGTGCAAAAGCAAAATGCGATCATTAAAACAAGTGCGTTA
AAAGGCCTATTCAAAAAAGACCCTAAAACTAGAGCTGAATTTATGCAACTCTTAAAATCTTAG

Upstream 100 bases:

>100_bases
TAGCACCCGATCAGCGGCTTATCTTTTTAACGCTGAAATGTTTAGCACCCACCCTAGCATTAAAAATCGTATCCAATCTT
TATCAAGGCGCGCTCTTTAA

Downstream 100 bases:

>100_bases
GCTATAATTCTATGCATGAATAACCCTAATTTATCCTTTTATTATAACGAGTGCGAGCGTTTTGAAAGCTTTTTAAAAAA
CCATCGTCTACACCTTGAAA

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I

Number of amino acids: Translated: 180; Mature: 180

Protein sequence:

>180_residues
MENFFNQFFENIGEDKNREGLKNTPKRVQELWNFLYKGYKEDPKVALKSAYFQGVCDEMIVAQHIEFYSTCEHHLLPFFG
NISLGYIPKEKIIGISAIAKLIEIYSKRLQIQERLTTQIAETFDEIIEPRGVIVICEAKHLCMSMQGVQKQNAIIKTSAL
KGLFKKDPKTRAEFMQLLKS

Sequences:

>Translated_180_residues
MENFFNQFFENIGEDKNREGLKNTPKRVQELWNFLYKGYKEDPKVALKSAYFQGVCDEMIVAQHIEFYSTCEHHLLPFFG
NISLGYIPKEKIIGISAIAKLIEIYSKRLQIQERLTTQIAETFDEIIEPRGVIVICEAKHLCMSMQGVQKQNAIIKTSAL
KGLFKKDPKTRAEFMQLLKS
>Mature_180_residues
MENFFNQFFENIGEDKNREGLKNTPKRVQELWNFLYKGYKEDPKVALKSAYFQGVCDEMIVAQHIEFYSTCEHHLLPFFG
NISLGYIPKEKIIGISAIAKLIEIYSKRLQIQERLTTQIAETFDEIIEPRGVIVICEAKHLCMSMQGVQKQNAIIKTSAL
KGLFKKDPKTRAEFMQLLKS

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family

Homologues:

Organism=Homo sapiens, GI66932968, Length=176, Percent_Identity=44.8863636363636, Blast_Score=181, Evalue=3e-46,
Organism=Homo sapiens, GI4503949, Length=176, Percent_Identity=44.8863636363636, Blast_Score=181, Evalue=3e-46,
Organism=Homo sapiens, GI66932970, Length=134, Percent_Identity=42.5373134328358, Blast_Score=130, Evalue=5e-31,
Organism=Homo sapiens, GI66932972, Length=134, Percent_Identity=42.5373134328358, Blast_Score=130, Evalue=6e-31,
Organism=Escherichia coli, GI1788476, Length=168, Percent_Identity=32.1428571428571, Blast_Score=103, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI17560486, Length=178, Percent_Identity=45.5056179775281, Blast_Score=184, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6321706, Length=172, Percent_Identity=46.5116279069767, Blast_Score=167, Evalue=7e-43,
Organism=Drosophila melanogaster, GI24656782, Length=180, Percent_Identity=44.4444444444444, Blast_Score=168, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24656796, Length=180, Percent_Identity=44.4444444444444, Blast_Score=168, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24656791, Length=180, Percent_Identity=44.4444444444444, Blast_Score=168, Evalue=2e-42,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): GCH1_HELAH (Q17WX5)

Other databases:

- EMBL:   AM260522
- RefSeq:   YP_664850.1
- ProteinModelPortal:   Q17WX5
- SMR:   Q17WX5
- STRING:   Q17WX5
- GeneID:   4177511
- GenomeReviews:   AM260522_GR
- KEGG:   hac:Hac_1087
- NMPDR:   fig|382638.8.peg.1056
- eggNOG:   COG0302
- HOGENOM:   HBG370191
- OMA:   IQERLTT
- PhylomeDB:   Q17WX5
- ProtClustDB:   PRK09347
- BioCyc:   HACI382638:HAC_1087-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00223
- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234
- TIGRFAMs:   TIGR00063

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =3.5.4.16

Molecular weight: Translated: 20842; Mature: 20842

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENFFNQFFENIGEDKNREGLKNTPKRVQELWNFLYKGYKEDPKVALKSAYFQGVCDEMI
CCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
VAQHIEFYSTCEHHLLPFFGNISLGYIPKEKIIGISAIAKLIEIYSKRLQIQERLTTQIA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ETFDEIIEPRGVIVICEAKHLCMSMQGVQKQNAIIKTSALKGLFKKDPKTRAEFMQLLKS
HHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHC
>Mature Secondary Structure
MENFFNQFFENIGEDKNREGLKNTPKRVQELWNFLYKGYKEDPKVALKSAYFQGVCDEMI
CCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
VAQHIEFYSTCEHHLLPFFGNISLGYIPKEKIIGISAIAKLIEIYSKRLQIQERLTTQIA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ETFDEIIEPRGVIVICEAKHLCMSMQGVQKQNAIIKTSALKGLFKKDPKTRAEFMQLLKS
HHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA