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The map label for this gene is gltX

Identifier: 109947606

GI number: 109947606

Start: 927270

End: 928589

Strand: Reverse

Name: gltX

Synonym: Hac_1071

Alternate gene names: 109947606

Gene position: 928589-927270 (Counterclockwise)

Preceding gene: 109947611

Following gene: 109947605

Centisome position: 59.76

GC content: 35.0

Gene sequence:

>1320_bases
ATGCTTCGTTTTGCACCTTCGCCTACTGGGGATATGCACATAGGGAATTTAAGGGCAGCCATTTTTAACTATATTGTGGC
TAAACAGCAACATAAACCCTTTCTCATTCGCATTGAAGACACAGATAAAGAGCGCAATATTGAAGGCAAAGATCAGGAGA
TTTTAGAGATTCTAAAGCTCATGGGAATGAACTGGGATAAACTCGTGTATCAAAGCCATAACATAGATTACCATAGGGAA
ATGGCAGAAAAATTGCTTAAAGAAAATAAGGCGTTTTATTGTTATGCGAGTGTGGGATTTTTAGAACAAGAAAAAGAAAA
AGCCAAAAACGAAAAACGCCCTTTCAGGTATTTAGATGAATGGGCGGCTTTAGAGAAAAACCAGCACAATACCCCTGTGG
TGCGTTTAAAAGCCCCAAATCATGCGGTGTCTTTTAACGATGCGATTAAAAAAGAAGTGAAATTTGAGCCTTATGAATTG
GATTCTTTTGTGCTTTTAAGAAAGGATAAGAGCCCTACTTATAATTTCGCTTGTGCATGCGATGATTTGCTTTATGAAAT
CAGTCTTATTATTAGGGGCGAAGATCATGTGAGTAACACCCCTAAACAAATCTTAATCCAACAAGCTTTAGGCTCCAATA
ACCCTATTATTTATGCACATTTGCCCATTATCTTAGATGAAGCAAGCGGTAAAAAGATGAGCAAAAGGGATGAAGCCTCT
AGCGTGAAATGGCTTTTAAATCAAGGGTTTTTGCCGGTTGCTATTGTGAATTACCTCATCACTATTGGTAATAAAGTGCC
TAAAGAAGTTTTTAGCCTTGATGAAGCGTTAGAATGGTTTAGTTTAGAAAACCTTTCTAATTCCCCAGCTCATTTTAATT
TAAAATATTTAAAACACTTAAACCACCAGCATTTAAAGCGTTTAGATGATGAAAAATTATTAGAGCTTTCTCAAATAAAA
GATAGGAATCTTTTAGGGCTTTTAAGATTATTCATAGAAGAATGCGATACGCTTTTAGAATTGAAAGAAAAAATTTCGTT
GTTTTTAGAGCCAAAAGATATTGTTAAAACTTATGAAAACGAAGATTTTAAAGAGCGCTGCTCAATACTTTTTAACGCCC
TAAAAAGCATGGATTTTCAAGCGTATAAGGATTTTGAAAGTTTTAAAAAAGAAGCCATGCGATTAAGCCAGCTTAAAGGT
AAAGATTTTTTTAAACCTTTGCGCATTCTTTTAATTGGGGATTCGCATGGCGTTGAATTGCCTTTGATTTTCCCTTATAT
TCAAAGCCATTATCAAGAAATTTTAAGGCTAAAAGCATGA

Upstream 100 bases:

>100_bases
AAAGCGATTTCTTTTAGTGAGTTTAAAATTTTTATGTATGGGCTTATTTATGTCTCTTTTTTAGGTATAATCAAAAACTA
TTTTTTCATAAAGGGTTTTT

Downstream 100 bases:

>100_bases
TATTTTCCACTCTTCTTAATGCGATAGCCGTGATTTTAAGCTCGCTCATTACAATTTATATATGGGTAGTGATCATTTAT
TCGCTCATAAGCTTTGTGCA

Product: glutamylglutaminyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 1; GluRS 1

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MLRFAPSPTGDMHIGNLRAAIFNYIVAKQQHKPFLIRIEDTDKERNIEGKDQEILEILKLMGMNWDKLVYQSHNIDYHRE
MAEKLLKENKAFYCYASVGFLEQEKEKAKNEKRPFRYLDEWAALEKNQHNTPVVRLKAPNHAVSFNDAIKKEVKFEPYEL
DSFVLLRKDKSPTYNFACACDDLLYEISLIIRGEDHVSNTPKQILIQQALGSNNPIIYAHLPIILDEASGKKMSKRDEAS
SVKWLLNQGFLPVAIVNYLITIGNKVPKEVFSLDEALEWFSLENLSNSPAHFNLKYLKHLNHQHLKRLDDEKLLELSQIK
DRNLLGLLRLFIEECDTLLELKEKISLFLEPKDIVKTYENEDFKERCSILFNALKSMDFQAYKDFESFKKEAMRLSQLKG
KDFFKPLRILLIGDSHGVELPLIFPYIQSHYQEILRLKA

Sequences:

>Translated_439_residues
MLRFAPSPTGDMHIGNLRAAIFNYIVAKQQHKPFLIRIEDTDKERNIEGKDQEILEILKLMGMNWDKLVYQSHNIDYHRE
MAEKLLKENKAFYCYASVGFLEQEKEKAKNEKRPFRYLDEWAALEKNQHNTPVVRLKAPNHAVSFNDAIKKEVKFEPYEL
DSFVLLRKDKSPTYNFACACDDLLYEISLIIRGEDHVSNTPKQILIQQALGSNNPIIYAHLPIILDEASGKKMSKRDEAS
SVKWLLNQGFLPVAIVNYLITIGNKVPKEVFSLDEALEWFSLENLSNSPAHFNLKYLKHLNHQHLKRLDDEKLLELSQIK
DRNLLGLLRLFIEECDTLLELKEKISLFLEPKDIVKTYENEDFKERCSILFNALKSMDFQAYKDFESFKKEAMRLSQLKG
KDFFKPLRILLIGDSHGVELPLIFPYIQSHYQEILRLKA
>Mature_439_residues
MLRFAPSPTGDMHIGNLRAAIFNYIVAKQQHKPFLIRIEDTDKERNIEGKDQEILEILKLMGMNWDKLVYQSHNIDYHRE
MAEKLLKENKAFYCYASVGFLEQEKEKAKNEKRPFRYLDEWAALEKNQHNTPVVRLKAPNHAVSFNDAIKKEVKFEPYEL
DSFVLLRKDKSPTYNFACACDDLLYEISLIIRGEDHVSNTPKQILIQQALGSNNPIIYAHLPIILDEASGKKMSKRDEAS
SVKWLLNQGFLPVAIVNYLITIGNKVPKEVFSLDEALEWFSLENLSNSPAHFNLKYLKHLNHQHLKRLDDEKLLELSQIK
DRNLLGLLRLFIEECDTLLELKEKISLFLEPKDIVKTYENEDFKERCSILFNALKSMDFQAYKDFESFKKEAMRLSQLKG
KDFFKPLRILLIGDSHGVELPLIFPYIQSHYQEILRLKA

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=354, Percent_Identity=30.225988700565, Blast_Score=155, Evalue=6e-38,
Organism=Homo sapiens, GI62241042, Length=259, Percent_Identity=24.3243243243243, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1788743, Length=432, Percent_Identity=33.1018518518519, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI226510925, Length=242, Percent_Identity=30.1652892561983, Blast_Score=114, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI71993394, Length=306, Percent_Identity=33.3333333333333, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17511007, Length=259, Percent_Identity=24.3243243243243, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6324540, Length=339, Percent_Identity=33.6283185840708, Blast_Score=166, Evalue=7e-42,
Organism=Drosophila melanogaster, GI24665321, Length=323, Percent_Identity=28.7925696594427, Blast_Score=144, Evalue=9e-35,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE1_HELAH (Q17WZ1)

Other databases:

- EMBL:   AM260522
- RefSeq:   YP_664834.1
- ProteinModelPortal:   Q17WZ1
- SMR:   Q17WZ1
- STRING:   Q17WZ1
- GeneID:   4177262
- GenomeReviews:   AM260522_GR
- KEGG:   hac:Hac_1071
- NMPDR:   fig|382638.8.peg.1040
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   SKRDDAS
- ProtClustDB:   PRK12410
- BioCyc:   HACI382638:HAC_1071-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 51279; Mature: 51279

Theoretical pI: Translated: 7.21; Mature: 7.21

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 235-235

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRFAPSPTGDMHIGNLRAAIFNYIVAKQQHKPFLIRIEDTDKERNIEGKDQEILEILKL
CCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHH
MGMNWDKLVYQSHNIDYHREMAEKLLKENKAFYCYASVGFLEQEKEKAKNEKRPFRYLDE
HCCCHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCCCHHHHHH
WAALEKNQHNTPVVRLKAPNHAVSFNDAIKKEVKFEPYELDSFVLLRKDKSPTYNFACAC
HHHHCCCCCCCCEEEEECCCCCCCCHHHHHHHCCCCCCCCCCEEEEECCCCCCCEEEEHH
DDLLYEISLIIRGEDHVSNTPKQILIQQALGSNNPIIYAHLPIILDEASGKKMSKRDEAS
HHHHHEEHHEEECCHHCCCCHHHHHHHHHHCCCCCEEEEEECEEEECCCCCCCCHHHHHH
SVKWLLNQGFLPVAIVNYLITIGNKVPKEVFSLDEALEWFSLENLSNSPAHFNLKYLKHL
HHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHC
NHQHLKRLDDEKLLELSQIKDRNLLGLLRLFIEECDTLLELKEKISLFLEPKDIVKTYEN
CHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHCC
EDFKERCSILFNALKSMDFQAYKDFESFKKEAMRLSQLKGKDFFKPLRILLIGDSHGVEL
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCEEEEEEECCCCCCC
PLIFPYIQSHYQEILRLKA
CEEHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLRFAPSPTGDMHIGNLRAAIFNYIVAKQQHKPFLIRIEDTDKERNIEGKDQEILEILKL
CCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHH
MGMNWDKLVYQSHNIDYHREMAEKLLKENKAFYCYASVGFLEQEKEKAKNEKRPFRYLDE
HCCCHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCCCHHHHHH
WAALEKNQHNTPVVRLKAPNHAVSFNDAIKKEVKFEPYELDSFVLLRKDKSPTYNFACAC
HHHHCCCCCCCCEEEEECCCCCCCCHHHHHHHCCCCCCCCCCEEEEECCCCCCCEEEEHH
DDLLYEISLIIRGEDHVSNTPKQILIQQALGSNNPIIYAHLPIILDEASGKKMSKRDEAS
HHHHHEEHHEEECCHHCCCCHHHHHHHHHHCCCCCEEEEEECEEEECCCCCCCCHHHHHH
SVKWLLNQGFLPVAIVNYLITIGNKVPKEVFSLDEALEWFSLENLSNSPAHFNLKYLKHL
HHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHC
NHQHLKRLDDEKLLELSQIKDRNLLGLLRLFIEECDTLLELKEKISLFLEPKDIVKTYEN
CHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHCC
EDFKERCSILFNALKSMDFQAYKDFESFKKEAMRLSQLKGKDFFKPLRILLIGDSHGVEL
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCEEEEEEECCCCCCC
PLIFPYIQSHYQEILRLKA
CEEHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA