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The map label for this gene is slt [H]

Identifier: 109947604

GI number: 109947604

Start: 925292

End: 926971

Strand: Reverse

Name: slt [H]

Synonym: Hac_1069

Alternate gene names: 109947604

Gene position: 926971-925292 (Counterclockwise)

Preceding gene: 109947605

Following gene: 109947603

Centisome position: 59.65

GC content: 36.37

Gene sequence:

>1680_bases
ATGCGTTTTTTTATTTTATTTTTTATGGGTGTGCTTGGCGTTGGTTTTTCTCAAACCGAGTTGAATTTAAAAGATTTAGA
GAAAAAGCCCATTGGGATTGTTAGGGATTATTATTTATGGCGTTATATTAGTGATAAAAAAACTAGTTTAGAAAACGCTA
AAAAAGCCTATGAATTGACTCAAAATAAAAATAGTGCCCTGCAAAAAGCCATGCAAGAAAAAGGCTCGGACAATTCAGAG
AAGATCCCTGATGCCAAAATGCCTGAAGATATTTATTGCAAGCAAATTACTTTAGAAAGCATGCTAGAAGAATTAGACAC
TTTCCAAAATAGCTGTATCGCTATCGCTTTAAAATCAAAAATCAAGGATTTTGATAAAATCCCTATTCAAACCCTTAAGC
CCTTACAAGCTAAAATCAAAGAGGCTTATCCTGTTCTTTATGAAGAATTAGAAATTTTGCAAAGTAAGAATGTGAGCGCT
TCTTTGTTTAAGGCTAATGTGCAAGTGTTTAGTGCGCTTTTTAATCATTTGAGTTACGAAAAAAAGCTCCAACTCTTTGA
AGAGCATATCCCTATTAAGGAATTAAACCGCCTTTTAGATGAAAATTACCCAGCATTTAACCGCTTGATTTATCAAGTTA
TATTAGATCCTAAATTGGATCATTTTAAAGACGCTCTAGCTAAAAGTAACGCCACCCACAGCAACGCACAAACCTTTTTT
ATTTTAGGGATTAATGAAATTTTACGCAAAAAACCCTCTAAAGCGCTCAAATATTTTGAACGATCCGAAGAAGTTGTCAA
AGACGATGATTTTTCAAAAAATAGAGCCATCTTTTGGCAATACCTAGTCTCTAAAAAGAAAAAAACTTTAGAACGCCTTT
CACAAAGCCCTGCCTTAAACCTTTATAGCTTTTATGCAAACCGCAAACTCAAAGCTGCACCTAATTACCGCATCATTTCG
CATATCCAAAATTTAAGCCAAGAAGCCCCTCCCTTTAACACTTACGATCCTTTTTTATGGCAAATTTTTAAGGAAAAAAC
TTTGAGTTTGAAAGATGAGGGGGCGTTTAATGCGATGCTAAAGAGCTTGCATTATGAAAAAAGCGCTCCTGAATTAACCT
ATTTTTTAAGCCAACGCAATAAAGAAAAGCTTTATTATTATTTATCCCCTTATGAGGGTATTATTGAGTGGCAAAGCGTG
GATGAAAAGGCGATGGCGTATGCGATCGCTAGGCAAGAAAGCTTTTTGCTCCCGGCGGTCATTTCGCGCTCCTTTGCTCT
AGGACTTATGCAAATCATGCCCTTTAATGTAGGGCCTTTCGCTAAAAGCCTTGGCATGGATAATATTGATCTAAACGACA
TGTTTAACCCCAATATCGCCCTTAAATTTGGCAATTATTATTTGAACCGTTTGAAAAAACAATTCAACCACCCCCTTTTT
GTCGCCTATGCTTATAACGCTGGGCCGGGGTTTTTAAGGAGATGGTTAGAAAGCTCCAAACGATTCAAAGGAAAAAATCG
CTTTGAGCCTTGGCTTAGCATGGAGCTTATGCCTTATAGCGAAACCCGTATGTATGGCTTTAGGGTCATGCTCAATTACT
TGATTTATCAAGAAATTTTTGGGAATTTCATCCCCATTGATGCATTTTTAGAACAAACTCTTACAAAGGACAACTCATGA

Upstream 100 bases:

>100_bases
TGGATTTCGCTCCTTTAGTGGTGGTCATTGTTTTGAAGTTTTTAGATTTGACTTTAATCCAATGGCTTTTTATGCTCGCC
AAAAGTCTCTAAAGAAAAGC

Downstream 100 bases:

>100_bases
TTAAAAAATGCCTTTTTCCTGCCGCCGGCTATGGCACACGCTTTTTGCCGATCACTAAAACCATCCCTAAAGAGATGCTA
CCCATTGTGGATAAACCTCT

Product: lytic murein transglycosylase

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 559; Mature: 559

Protein sequence:

>559_residues
MRFFILFFMGVLGVGFSQTELNLKDLEKKPIGIVRDYYLWRYISDKKTSLENAKKAYELTQNKNSALQKAMQEKGSDNSE
KIPDAKMPEDIYCKQITLESMLEELDTFQNSCIAIALKSKIKDFDKIPIQTLKPLQAKIKEAYPVLYEELEILQSKNVSA
SLFKANVQVFSALFNHLSYEKKLQLFEEHIPIKELNRLLDENYPAFNRLIYQVILDPKLDHFKDALAKSNATHSNAQTFF
ILGINEILRKKPSKALKYFERSEEVVKDDDFSKNRAIFWQYLVSKKKKTLERLSQSPALNLYSFYANRKLKAAPNYRIIS
HIQNLSQEAPPFNTYDPFLWQIFKEKTLSLKDEGAFNAMLKSLHYEKSAPELTYFLSQRNKEKLYYYLSPYEGIIEWQSV
DEKAMAYAIARQESFLLPAVISRSFALGLMQIMPFNVGPFAKSLGMDNIDLNDMFNPNIALKFGNYYLNRLKKQFNHPLF
VAYAYNAGPGFLRRWLESSKRFKGKNRFEPWLSMELMPYSETRMYGFRVMLNYLIYQEIFGNFIPIDAFLEQTLTKDNS

Sequences:

>Translated_559_residues
MRFFILFFMGVLGVGFSQTELNLKDLEKKPIGIVRDYYLWRYISDKKTSLENAKKAYELTQNKNSALQKAMQEKGSDNSE
KIPDAKMPEDIYCKQITLESMLEELDTFQNSCIAIALKSKIKDFDKIPIQTLKPLQAKIKEAYPVLYEELEILQSKNVSA
SLFKANVQVFSALFNHLSYEKKLQLFEEHIPIKELNRLLDENYPAFNRLIYQVILDPKLDHFKDALAKSNATHSNAQTFF
ILGINEILRKKPSKALKYFERSEEVVKDDDFSKNRAIFWQYLVSKKKKTLERLSQSPALNLYSFYANRKLKAAPNYRIIS
HIQNLSQEAPPFNTYDPFLWQIFKEKTLSLKDEGAFNAMLKSLHYEKSAPELTYFLSQRNKEKLYYYLSPYEGIIEWQSV
DEKAMAYAIARQESFLLPAVISRSFALGLMQIMPFNVGPFAKSLGMDNIDLNDMFNPNIALKFGNYYLNRLKKQFNHPLF
VAYAYNAGPGFLRRWLESSKRFKGKNRFEPWLSMELMPYSETRMYGFRVMLNYLIYQEIFGNFIPIDAFLEQTLTKDNS
>Mature_559_residues
MRFFILFFMGVLGVGFSQTELNLKDLEKKPIGIVRDYYLWRYISDKKTSLENAKKAYELTQNKNSALQKAMQEKGSDNSE
KIPDAKMPEDIYCKQITLESMLEELDTFQNSCIAIALKSKIKDFDKIPIQTLKPLQAKIKEAYPVLYEELEILQSKNVSA
SLFKANVQVFSALFNHLSYEKKLQLFEEHIPIKELNRLLDENYPAFNRLIYQVILDPKLDHFKDALAKSNATHSNAQTFF
ILGINEILRKKPSKALKYFERSEEVVKDDDFSKNRAIFWQYLVSKKKKTLERLSQSPALNLYSFYANRKLKAAPNYRIIS
HIQNLSQEAPPFNTYDPFLWQIFKEKTLSLKDEGAFNAMLKSLHYEKSAPELTYFLSQRNKEKLYYYLSPYEGIIEWQSV
DEKAMAYAIARQESFLLPAVISRSFALGLMQIMPFNVGPFAKSLGMDNIDLNDMFNPNIALKFGNYYLNRLKKQFNHPLF
VAYAYNAGPGFLRRWLESSKRFKGKNRFEPWLSMELMPYSETRMYGFRVMLNYLIYQEIFGNFIPIDAFLEQTLTKDNS

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=145, Percent_Identity=35.8620689655172, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 65385; Mature: 65385

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure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CCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]