Click here to switch to the map view.

The map label for this gene is aspA [H]

Identifier: 109947600

GI number: 109947600

Start: 921324

End: 922730

Strand: Reverse

Name: aspA [H]

Synonym: Hac_1065

Alternate gene names: 109947600

Gene position: 922730-921324 (Counterclockwise)

Preceding gene: 109947601

Following gene: 109947599

Centisome position: 59.38

GC content: 41.72

Gene sequence:

>1407_bases
ATGCGTATTGAGCATGATTTCATTGGAGAGATGGAAATTAGCGATGAGGTTTATTATGGGGTTCAAACTTTAAGGGCGAG
TGAAAATTTTTTCATCACCAACGACAAGCTTTGCAGTTATCCTATTTTTATCAAATCTTTCGCTCAAGTCAAAAAAGCGG
CCGCTTTAGCAAATGCGCAATTAGGCTTGATTGATGAAAAGGTTAAGGTTGCGATTTGCCATGCGTGCGATCTATTGATT
GATGGCAAGCACCATGATCAATTCATTGTGGATATGATTCAAGGGGGGGCTGGCACGAGCACGAACATGAACATGAATGA
AGTCGTTGCTAATTTGGCTTTAGAATACATGGGGCATAAAAAGGGCGAGTATCAATTTTGCCACCCAAACGATCATGTCA
ATCGCTCTCAATCCACCAATGACGCCTATCCTAGCGCGTTAAAAATTGCGATTTATGAGCGTTTGAGTAATCTAGTCAAT
CCCATGAAAGCCTTAAGGGATGCTTTCACTCAAAAGGCTAAAGAATTTGCTCATGTGATTAAAATGGGGCGCACCCAGCT
TCAAGACGCTGTGCCTATGACTTTAGGGCAGGAGTTTGAAACTTATGCTTTGATGGTTGATAGGGATATTGAGCATGTTT
TAGATGCTAGAAATTGGGTAAGAGAGCTTAATTTGGGTGGCACCGCTATTGGCACAGGGATCAATTCGCATCCGGATTAT
CGCAACTTGATTGAAAAGAAAATCCAAGAAGTGACAGGTCGTCCTTTTATCATGGCTAATAATTTGATAGAAGCCACTCA
AAGCACTGGGGCGTATGTGCAAGTGAGTGGGGTGTTAAAGCGTATTGCAATCAAACTTTCTAAGGTTTGTAACGATTTAA
GACTGCTCAGTTCAGGGCCTAGAGCCGGACTGAATGAAATCAATTTGCCTAAAATGCAACCTGGAAGCTCTATTATGCCG
GGTAAAGTCAATCCGGTGATCCCTGAAGTGGTCAATCAAGTGTGCTTTGCGGTCATTGGGAATGATTTGAGCGTGGCGTT
AGCTGCAGAAGGTGGGCAGTTACAACTCAATGTGTTTGAGCCGGTTATCGCTTACAAGCTCTTCCATTCCTTTGTGATTT
TAGGGCGTGCGATTGAAACTTTAACGACTAAATGCGTGGAAGGCATCACGGCTAATGAAAAGATTTGCAAAGATTATGTC
TTTAATAGCATTGGTATCGTTACCGCACTCAACCCTCACATTGGCTATGAAAAATCCGCTATGATCGCTAAAGAAGCCTT
AAAAAGCGGTCGTTCTATCTATGACATCGCTTTAGAAAAGAAAATCTTAACTAAAGAGCAGTTAGACGATATTTTTAAGC
CAGAAAACATGCTAAGCCCTCACGCTTTCAAAAAGCATAAAGATTAA

Upstream 100 bases:

>100_bases
AATCAACGCTTTAGGGGCAAAAGTGTTGCGTTTAAAAGAAAAATAACCCAAGATTTGGCTACAATAACTAGAATATTTTT
TAACGATACAAGGAGCTTTT

Downstream 100 bases:

>100_bases
ACGCTTTGCAACACTCACGCCTTCAAGTTTTACGCTCCCTTTATATGGAGCGTCTTTTAGGCGAAACTTACACTAACATC
AATCTTACAAAACCCCAAAA

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MRIEHDFIGEMEISDEVYYGVQTLRASENFFITNDKLCSYPIFIKSFAQVKKAAALANAQLGLIDEKVKVAICHACDLLI
DGKHHDQFIVDMIQGGAGTSTNMNMNEVVANLALEYMGHKKGEYQFCHPNDHVNRSQSTNDAYPSALKIAIYERLSNLVN
PMKALRDAFTQKAKEFAHVIKMGRTQLQDAVPMTLGQEFETYALMVDRDIEHVLDARNWVRELNLGGTAIGTGINSHPDY
RNLIEKKIQEVTGRPFIMANNLIEATQSTGAYVQVSGVLKRIAIKLSKVCNDLRLLSSGPRAGLNEINLPKMQPGSSIMP
GKVNPVIPEVVNQVCFAVIGNDLSVALAAEGGQLQLNVFEPVIAYKLFHSFVILGRAIETLTTKCVEGITANEKICKDYV
FNSIGIVTALNPHIGYEKSAMIAKEALKSGRSIYDIALEKKILTKEQLDDIFKPENMLSPHAFKKHKD

Sequences:

>Translated_468_residues
MRIEHDFIGEMEISDEVYYGVQTLRASENFFITNDKLCSYPIFIKSFAQVKKAAALANAQLGLIDEKVKVAICHACDLLI
DGKHHDQFIVDMIQGGAGTSTNMNMNEVVANLALEYMGHKKGEYQFCHPNDHVNRSQSTNDAYPSALKIAIYERLSNLVN
PMKALRDAFTQKAKEFAHVIKMGRTQLQDAVPMTLGQEFETYALMVDRDIEHVLDARNWVRELNLGGTAIGTGINSHPDY
RNLIEKKIQEVTGRPFIMANNLIEATQSTGAYVQVSGVLKRIAIKLSKVCNDLRLLSSGPRAGLNEINLPKMQPGSSIMP
GKVNPVIPEVVNQVCFAVIGNDLSVALAAEGGQLQLNVFEPVIAYKLFHSFVILGRAIETLTTKCVEGITANEKICKDYV
FNSIGIVTALNPHIGYEKSAMIAKEALKSGRSIYDIALEKKILTKEQLDDIFKPENMLSPHAFKKHKD
>Mature_468_residues
MRIEHDFIGEMEISDEVYYGVQTLRASENFFITNDKLCSYPIFIKSFAQVKKAAALANAQLGLIDEKVKVAICHACDLLI
DGKHHDQFIVDMIQGGAGTSTNMNMNEVVANLALEYMGHKKGEYQFCHPNDHVNRSQSTNDAYPSALKIAIYERLSNLVN
PMKALRDAFTQKAKEFAHVIKMGRTQLQDAVPMTLGQEFETYALMVDRDIEHVLDARNWVRELNLGGTAIGTGINSHPDY
RNLIEKKIQEVTGRPFIMANNLIEATQSTGAYVQVSGVLKRIAIKLSKVCNDLRLLSSGPRAGLNEINLPKMQPGSSIMP
GKVNPVIPEVVNQVCFAVIGNDLSVALAAEGGQLQLNVFEPVIAYKLFHSFVILGRAIETLTTKCVEGITANEKICKDYV
FNSIGIVTALNPHIGYEKSAMIAKEALKSGRSIYDIALEKKILTKEQLDDIFKPENMLSPHAFKKHKD

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=461, Percent_Identity=43.8177874186551, Blast_Score=377, Evalue=1e-104,
Organism=Escherichia coli, GI87082375, Length=470, Percent_Identity=55.7446808510638, Blast_Score=521, Evalue=1e-149,
Organism=Escherichia coli, GI1787896, Length=459, Percent_Identity=40.0871459694989, Blast_Score=363, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17553882, Length=462, Percent_Identity=43.5064935064935, Blast_Score=384, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI32565146, Length=326, Percent_Identity=43.558282208589, Blast_Score=279, Evalue=3e-75,
Organism=Saccharomyces cerevisiae, GI6324993, Length=461, Percent_Identity=41.648590021692, Blast_Score=364, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24640177, Length=461, Percent_Identity=43.6008676789588, Blast_Score=376, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24640179, Length=462, Percent_Identity=43.5064935064935, Blast_Score=376, Evalue=1e-104,
Organism=Drosophila melanogaster, GI78710009, Length=463, Percent_Identity=43.1965442764579, Blast_Score=367, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24662684, Length=462, Percent_Identity=42.8571428571429, Blast_Score=366, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24583245, Length=463, Percent_Identity=37.1490280777538, Blast_Score=311, Evalue=5e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR003031
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 51927; Mature: 51927

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIEHDFIGEMEISDEVYYGVQTLRASENFFITNDKLCSYPIFIKSFAQVKKAAALANAQ
CCCCCCCCCCEECCCHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCC
LGLIDEKVKVAICHACDLLIDGKHHDQFIVDMIQGGAGTSTNMNMNEVVANLALEYMGHK
CCCCCHHHHHHHHHHHHHEECCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCC
KGEYQFCHPNDHVNRSQSTNDAYPSALKIAIYERLSNLVNPMKALRDAFTQKAKEFAHVI
CCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KMGRTQLQDAVPMTLGQEFETYALMVDRDIEHVLDARNWVRELNLGGTAIGTGINSHPDY
HHHHHHHHHHCCHHHCCCHHHEEEEEHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCHH
RNLIEKKIQEVTGRPFIMANNLIEATQSTGAYVQVSGVLKRIAIKLSKVCNDLRLLSSGP
HHHHHHHHHHHCCCCEEEECHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCC
RAGLNEINLPKMQPGSSIMPGKVNPVIPEVVNQVCFAVIGNDLSVALAAEGGQLQLNVFE
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEHHH
PVIAYKLFHSFVILGRAIETLTTKCVEGITANEKICKDYVFNSIGIVTALNPHIGYEKSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEECCCCCCCHHH
MIAKEALKSGRSIYDIALEKKILTKEQLDDIFKPENMLSPHAFKKHKD
HHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCC
>Mature Secondary Structure
MRIEHDFIGEMEISDEVYYGVQTLRASENFFITNDKLCSYPIFIKSFAQVKKAAALANAQ
CCCCCCCCCCEECCCHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCC
LGLIDEKVKVAICHACDLLIDGKHHDQFIVDMIQGGAGTSTNMNMNEVVANLALEYMGHK
CCCCCHHHHHHHHHHHHHEECCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCC
KGEYQFCHPNDHVNRSQSTNDAYPSALKIAIYERLSNLVNPMKALRDAFTQKAKEFAHVI
CCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KMGRTQLQDAVPMTLGQEFETYALMVDRDIEHVLDARNWVRELNLGGTAIGTGINSHPDY
HHHHHHHHHHCCHHHCCCHHHEEEEEHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCHH
RNLIEKKIQEVTGRPFIMANNLIEATQSTGAYVQVSGVLKRIAIKLSKVCNDLRLLSSGP
HHHHHHHHHHHCCCCEEEECHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCC
RAGLNEINLPKMQPGSSIMPGKVNPVIPEVVNQVCFAVIGNDLSVALAAEGGQLQLNVFE
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEHHH
PVIAYKLFHSFVILGRAIETLTTKCVEGITANEKICKDYVFNSIGIVTALNPHIGYEKSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEECCCCCCCHHH
MIAKEALKSGRSIYDIALEKKILTKEQLDDIFKPENMLSPHAFKKHKD
HHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]