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The map label for this gene is hemL

Identifier: 109947557

GI number: 109947557

Start: 887666

End: 888958

Strand: Reverse

Name: hemL

Synonym: Hac_1022

Alternate gene names: 109947557

Gene position: 888958-887666 (Counterclockwise)

Preceding gene: 109947558

Following gene: 109947556

Centisome position: 57.21

GC content: 42.46

Gene sequence:

>1293_bases
ATGGAGTTGTTGCATAGTATCAATGATTTTAATGAAGCCAAACAGGTGATCGTTGGGGGGGTCAATTCGCCTGTGAGGGC
GTTTAAGAGTGTTAAAGGCACTCCGCCTTTTATCTTAAAGGGTAAGGGGGCGTATCTTTATGATGTGGATAACAACCATT
ATATAGATTTTGTGCAAAGTTGGGGACCTTTGATTTTTGGGCATGCTGATGAGGAGATTGAAGAAAATATCATTAAAGCG
TTAAAAAAAGGCACTTCTTTTGGTGCTCCCACAGAGTTGGAAACCACTTTAGCTAAAGAAATCATTTCTTGTTATGATGG
CTTAGATAAGGTGCGTTTAGTCAATAGCGGCACGGAAGCGACGATGAGTGCGATACGGCTCGCTAGAGCTTATAGCCAAA
AAGACGATTTGATTAAGTTTGAAGGGTGCTACCATGGGCATAGCGATTCTTTGTTAGTGAAAGCGGGTAGTGGGTGCACT
ACTTTTGGCTCTCCTTCTTCTTTAGGCGTGCCGAGCGATTTTAGCAAGCACACTCTAGTGGCTCGTTATAACGATTTAAA
CTCTGTTCAAGAATGCTTTCAAAAAGGCAATGTGGGTTGTGTCATCATTGAACCCATTGCAGGAAATATGGGGCTAGTGC
CAGCCCAAAAAGAGTTTTTGGTGGGGTTAAAAGCCTTGTGTGAAAAATACCAAGCGGTGCTAATCTTAGATGAAGTGATG
AGCGGTTTTAGGGCGAGTTTGAGCGGTTCGCAAGAATTTTATGGCGTTGTGCCGGATTTAGTAACCTTTGGTAAGGTGAT
AGGTGCAGGGCTTCCTTTGGCGTGTTTTGGGGGGCGTGCGGAAATTATGGACTTGCTTTCGCCCATTGGGGGGGTGTATC
AAGCCGGTACTTTAAGCGGTAACCCTTTGGCGGTGTGTGCGGGATTGAGCACGTTGTATAAAATCAAAAGAGACAAAACG
CTTTATTCTCGCTTGAACGCTTTAGCCATTCGTTTGACTCAAGGTCTAAAAAAGAGTGCAAAATCTTACAACATCGCTTT
ACAAACGCTTAATAGAGGGAGTATGTTTGGCTTTTTCTTTAACGAAAATGCGGTGTGCGATTTTGATGATGCTTTAAAAA
GCAATACAGAAATGTTTGCGAAATTCCATCAAAAAATGCTCTTTAAAGGCGTGTATTTGGCATGTTCAAGCTTTGAAACC
GGCTTTATTTGTGAGCCTATGACTGAAGAGATGATTGATTTGGTGATCGTAAAAGCGAATGAGAGTTTTGATGAAATCAT
AAAGGGCGTGTGA

Upstream 100 bases:

>100_bases
AGCGAGCTTAGCCAAACGATGCGAGAGTTTTCATGAGAGCTTGACCTGGTCGCAAGTGGAGATCCAATTTGAAAGCATGA
TCAAGGGATAATTTAAAATC

Downstream 100 bases:

>100_bases
ATTTTTGAAAAAGCCAAAATACTACAAGTTTATAGAGGGGGCGAATTATTTGAGCTTGGGGCTTTCTATGGTTGTAGCGA
TCCTTATAGGTGTGGCTATA

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MELLHSINDFNEAKQVIVGGVNSPVRAFKSVKGTPPFILKGKGAYLYDVDNNHYIDFVQSWGPLIFGHADEEIEENIIKA
LKKGTSFGAPTELETTLAKEIISCYDGLDKVRLVNSGTEATMSAIRLARAYSQKDDLIKFEGCYHGHSDSLLVKAGSGCT
TFGSPSSLGVPSDFSKHTLVARYNDLNSVQECFQKGNVGCVIIEPIAGNMGLVPAQKEFLVGLKALCEKYQAVLILDEVM
SGFRASLSGSQEFYGVVPDLVTFGKVIGAGLPLACFGGRAEIMDLLSPIGGVYQAGTLSGNPLAVCAGLSTLYKIKRDKT
LYSRLNALAIRLTQGLKKSAKSYNIALQTLNRGSMFGFFFNENAVCDFDDALKSNTEMFAKFHQKMLFKGVYLACSSFET
GFICEPMTEEMIDLVIVKANESFDEIIKGV

Sequences:

>Translated_430_residues
MELLHSINDFNEAKQVIVGGVNSPVRAFKSVKGTPPFILKGKGAYLYDVDNNHYIDFVQSWGPLIFGHADEEIEENIIKA
LKKGTSFGAPTELETTLAKEIISCYDGLDKVRLVNSGTEATMSAIRLARAYSQKDDLIKFEGCYHGHSDSLLVKAGSGCT
TFGSPSSLGVPSDFSKHTLVARYNDLNSVQECFQKGNVGCVIIEPIAGNMGLVPAQKEFLVGLKALCEKYQAVLILDEVM
SGFRASLSGSQEFYGVVPDLVTFGKVIGAGLPLACFGGRAEIMDLLSPIGGVYQAGTLSGNPLAVCAGLSTLYKIKRDKT
LYSRLNALAIRLTQGLKKSAKSYNIALQTLNRGSMFGFFFNENAVCDFDDALKSNTEMFAKFHQKMLFKGVYLACSSFET
GFICEPMTEEMIDLVIVKANESFDEIIKGV
>Mature_430_residues
MELLHSINDFNEAKQVIVGGVNSPVRAFKSVKGTPPFILKGKGAYLYDVDNNHYIDFVQSWGPLIFGHADEEIEENIIKA
LKKGTSFGAPTELETTLAKEIISCYDGLDKVRLVNSGTEATMSAIRLARAYSQKDDLIKFEGCYHGHSDSLLVKAGSGCT
TFGSPSSLGVPSDFSKHTLVARYNDLNSVQECFQKGNVGCVIIEPIAGNMGLVPAQKEFLVGLKALCEKYQAVLILDEVM
SGFRASLSGSQEFYGVVPDLVTFGKVIGAGLPLACFGGRAEIMDLLSPIGGVYQAGTLSGNPLAVCAGLSTLYKIKRDKT
LYSRLNALAIRLTQGLKKSAKSYNIALQTLNRGSMFGFFFNENAVCDFDDALKSNTEMFAKFHQKMLFKGVYLACSSFET
GFICEPMTEEMIDLVIVKANESFDEIIKGV

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily

Homologues:

Organism=Homo sapiens, GI4557809, Length=309, Percent_Identity=26.537216828479, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI13994255, Length=363, Percent_Identity=25.6198347107438, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI284507298, Length=228, Percent_Identity=26.3157894736842, Blast_Score=79, Evalue=8e-15,
Organism=Escherichia coli, GI1786349, Length=424, Percent_Identity=50.7075471698113, Blast_Score=441, Evalue=1e-125,
Organism=Escherichia coli, GI1789016, Length=386, Percent_Identity=29.0155440414508, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1787560, Length=380, Percent_Identity=29.7368421052632, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1788044, Length=321, Percent_Identity=32.0872274143302, Blast_Score=127, Evalue=9e-31,
Organism=Escherichia coli, GI1789759, Length=333, Percent_Identity=28.5285285285285, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI145693181, Length=393, Percent_Identity=25.9541984732824, Blast_Score=94, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71992977, Length=342, Percent_Identity=29.2397660818713, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI25144271, Length=300, Percent_Identity=26.6666666666667, Blast_Score=95, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI25144274, Length=139, Percent_Identity=30.9352517985612, Blast_Score=68, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6323470, Length=336, Percent_Identity=28.8690476190476, Blast_Score=100, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6324432, Length=347, Percent_Identity=25.9365994236311, Blast_Score=87, Evalue=5e-18,
Organism=Drosophila melanogaster, GI21357415, Length=295, Percent_Identity=24.0677966101695, Blast_Score=86, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSA_HELAH (Q17X40)

Other databases:

- EMBL:   AM260522
- RefSeq:   YP_664785.1
- ProteinModelPortal:   Q17X40
- SMR:   Q17X40
- STRING:   Q17X40
- GeneID:   4177428
- GenomeReviews:   AM260522_GR
- KEGG:   hac:Hac_1022
- NMPDR:   fig|382638.8.peg.991
- eggNOG:   COG0001
- HOGENOM:   HBG725944
- OMA:   DKVIKFE
- PhylomeDB:   Q17X40
- ProtClustDB:   PRK00062
- BioCyc:   HACI382638:HAC_1022-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00375
- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11986
- TIGRFAMs:   TIGR00713

Pfam domain/function: PF00202 Aminotran_3; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =5.4.3.8

Molecular weight: Translated: 46740; Mature: 46740

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELLHSINDFNEAKQVIVGGVNSPVRAFKSVKGTPPFILKGKGAYLYDVDNNHYIDFVQS
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCEEEECCCEEEEECCCCCEEHHHHC
WGPLIFGHADEEIEENIIKALKKGTSFGAPTELETTLAKEIISCYDGLDKVRLVNSGTEA
CCCEEECCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHEEEECCCHHH
TMSAIRLARAYSQKDDLIKFEGCYHGHSDSLLVKAGSGCTTFGSPSSLGVPSDFSKHTLV
HHHHHHHHHHHCCCCCCEEEECEECCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCEEE
ARYNDLNSVQECFQKGNVGCVIIEPIAGNMGLVPAQKEFLVGLKALCEKYQAVLILDEVM
EECCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SGFRASLSGSQEFYGVVPDLVTFGKVIGAGLPLACFGGRAEIMDLLSPIGGVYQAGTLSG
HHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHCCCEECCCCCC
NPLAVCAGLSTLYKIKRDKTLYSRLNALAIRLTQGLKKSAKSYNIALQTLNRGSMFGFFF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCEEEEEE
NENAVCDFDDALKSNTEMFAKFHQKMLFKGVYLACSSFETGFICEPMTEEMIDLVIVKAN
CCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCHHHHCEEEEEEECC
ESFDEIIKGV
CCHHHHHCCC
>Mature Secondary Structure
MELLHSINDFNEAKQVIVGGVNSPVRAFKSVKGTPPFILKGKGAYLYDVDNNHYIDFVQS
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCEEEECCCEEEEECCCCCEEHHHHC
WGPLIFGHADEEIEENIIKALKKGTSFGAPTELETTLAKEIISCYDGLDKVRLVNSGTEA
CCCEEECCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHEEEECCCHHH
TMSAIRLARAYSQKDDLIKFEGCYHGHSDSLLVKAGSGCTTFGSPSSLGVPSDFSKHTLV
HHHHHHHHHHHCCCCCCEEEECEECCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCEEE
ARYNDLNSVQECFQKGNVGCVIIEPIAGNMGLVPAQKEFLVGLKALCEKYQAVLILDEVM
EECCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SGFRASLSGSQEFYGVVPDLVTFGKVIGAGLPLACFGGRAEIMDLLSPIGGVYQAGTLSG
HHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHCCCEECCCCCC
NPLAVCAGLSTLYKIKRDKTLYSRLNALAIRLTQGLKKSAKSYNIALQTLNRGSMFGFFF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCEEEEEE
NENAVCDFDDALKSNTEMFAKFHQKMLFKGVYLACSSFETGFICEPMTEEMIDLVIVKAN
CCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCHHHHCEEEEEEECC
ESFDEIIKGV
CCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA