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The map label for this gene is rpoN [H]

Identifier: 109947484

GI number: 109947484

Start: 819945

End: 821189

Strand: Reverse

Name: rpoN [H]

Synonym: Hac_0937

Alternate gene names: 109947484

Gene position: 821189-819945 (Counterclockwise)

Preceding gene: 109947485

Following gene: 109947475

Centisome position: 52.85

GC content: 33.73

Gene sequence:

>1245_bases
ATGGCGGTTTTACGTGCAAACCTTAGCCCTAAAAACAAGTTAAACGCTACTTTAAAAGGGTGGCTACCTATTTTACAAAG
CGAGCTTGAAGATTTAGAAGAAGTTTTAAAACAAAATGCCCTAGATAACCCCTTAATCAAAATTGAAAACAAACGCATTA
AAAGCTTTAGCGATCGTTTTAACACTAAAAGTAGTAGCGATCATTTAGAAAATTTAACGATCGCATCAAAAAGCCTTTTT
GAGACTTTAAAAGATCAAATCATTCCCCCCCTTTTTCCCACTGAAACTTCTCAAAAAATCGCTATGGATATTATCAATGG
GTTAAATAATGAGGGGTATTTTGAAGAAAATGTTGAAGAAAGGGCTAAAATTTTAGGGGTAGAGAGCGAAATTTATGAGA
AAGTGCACAAGCGTTTTAGTTACCTTAATCCAGCTGGTATTGGAGCTAGAGATGTGAAAGAGAGCTTTTTATTTCAGCTA
GAGAGTAGGGAATTAGACAATAACGAGCTTTATGAAGAAACGCGAAAAATTATTTTGCATTTAGAAAAACACCATGAATT
TTCTAAAGATTTTTATTATGAAAAGGCTTTAAAAATTTTAAAATCCTTTAAAAACCCTCCAGCTATTGAGTTTTTAGAAA
AAGAAATAGAAGTCATTCCTGAGCTTTTTATTATGGAAGTGGATAATGAAATCATCGTTCGTTTGAATGATGAGAGCTAT
CCGACGATTAGTTTGGAAGAAAAGCGTTTTAAGAATAGCGATTATTTGAAAGATAAATTAAAAGAGGCTAAAGATTTGAT
TGACGCTTTAAATTTAAGGAAAGCCACGATTTATAAAATCGGACTCATGCTTTTGGAGTATCAATACGATTTTTTTAAAG
GAAAGGAATTGCGCCCTTTAAAATTATTGGATTTAGCCAATGAGTTTAACCACTCTGTAAGCACGATTTCAAGGGCAATT
TCTAATAAATATTTAGCATGCGAGAGAGGGGTTTTCCCTATTAAGCATTTCTTTAGCATCGCTTTAGACAATAGCGAGAC
TTCAAACGCCGTGATTAAAGACTACCTTTTAGAATTGATTAAAAACGAAGACAAAAAAGAGCCTTTGAGCGACGCTAAGA
TTTTAGAACTCATTGAAGAAAAATTCCACTTGAAAATGGTAAGAAGAACGATCACCAAATACCGCCAATTACTCAATATC
GCTTCATCAAGCGAAAGGAAAAAGCTCTATTTGATGCGCGCTTGA

Upstream 100 bases:

>100_bases
TTAAAAGTGGCACGCTTCTAGCGAGTGGGAACGCTAATGAAATTTATGAAAACGCTTTGGTGCGTAAATATTATTTAGGG
GAAAATTTTAAGGTGTGAAT

Downstream 100 bases:

>100_bases
AATCTTAAAACATGATTTAGGGTTTAATTATTTGTCTTTCTTTAAAGATAGCAGTATATCGTTTTATTTTTTCTAACTCT
ATTTTTTGTCAATCCTTAAT

Product: RNA polymerase factor sigma-54

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 414; Mature: 413

Protein sequence:

>414_residues
MAVLRANLSPKNKLNATLKGWLPILQSELEDLEEVLKQNALDNPLIKIENKRIKSFSDRFNTKSSSDHLENLTIASKSLF
ETLKDQIIPPLFPTETSQKIAMDIINGLNNEGYFEENVEERAKILGVESEIYEKVHKRFSYLNPAGIGARDVKESFLFQL
ESRELDNNELYEETRKIILHLEKHHEFSKDFYYEKALKILKSFKNPPAIEFLEKEIEVIPELFIMEVDNEIIVRLNDESY
PTISLEEKRFKNSDYLKDKLKEAKDLIDALNLRKATIYKIGLMLLEYQYDFFKGKELRPLKLLDLANEFNHSVSTISRAI
SNKYLACERGVFPIKHFFSIALDNSETSNAVIKDYLLELIKNEDKKEPLSDAKILELIEEKFHLKMVRRTITKYRQLLNI
ASSSERKKLYLMRA

Sequences:

>Translated_414_residues
MAVLRANLSPKNKLNATLKGWLPILQSELEDLEEVLKQNALDNPLIKIENKRIKSFSDRFNTKSSSDHLENLTIASKSLF
ETLKDQIIPPLFPTETSQKIAMDIINGLNNEGYFEENVEERAKILGVESEIYEKVHKRFSYLNPAGIGARDVKESFLFQL
ESRELDNNELYEETRKIILHLEKHHEFSKDFYYEKALKILKSFKNPPAIEFLEKEIEVIPELFIMEVDNEIIVRLNDESY
PTISLEEKRFKNSDYLKDKLKEAKDLIDALNLRKATIYKIGLMLLEYQYDFFKGKELRPLKLLDLANEFNHSVSTISRAI
SNKYLACERGVFPIKHFFSIALDNSETSNAVIKDYLLELIKNEDKKEPLSDAKILELIEEKFHLKMVRRTITKYRQLLNI
ASSSERKKLYLMRA
>Mature_413_residues
AVLRANLSPKNKLNATLKGWLPILQSELEDLEEVLKQNALDNPLIKIENKRIKSFSDRFNTKSSSDHLENLTIASKSLFE
TLKDQIIPPLFPTETSQKIAMDIINGLNNEGYFEENVEERAKILGVESEIYEKVHKRFSYLNPAGIGARDVKESFLFQLE
SRELDNNELYEETRKIILHLEKHHEFSKDFYYEKALKILKSFKNPPAIEFLEKEIEVIPELFIMEVDNEIIVRLNDESYP
TISLEEKRFKNSDYLKDKLKEAKDLIDALNLRKATIYKIGLMLLEYQYDFFKGKELRPLKLLDLANEFNHSVSTISRAIS
NKYLACERGVFPIKHFFSIALDNSETSNAVIKDYLLELIKNEDKKEPLSDAKILELIEEKFHLKMVRRTITKYRQLLNIA
SSSERKKLYLMRA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=382, Percent_Identity=28.7958115183246, Blast_Score=147, Evalue=2e-36,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 48362; Mature: 48231

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVLRANLSPKNKLNATLKGWLPILQSELEDLEEVLKQNALDNPLIKIENKRIKSFSDRF
CCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHC
NTKSSSDHLENLTIASKSLFETLKDQIIPPLFPTETSQKIAMDIINGLNNEGYFEENVEE
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHH
RAKILGVESEIYEKVHKRFSYLNPAGIGARDVKESFLFQLESRELDNNELYEETRKIILH
HHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
LEKHHEFSKDFYYEKALKILKSFKNPPAIEFLEKEIEVIPELFIMEVDNEIIVRLNDESY
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEEEECCEEEEEECCCCC
PTISLEEKRFKNSDYLKDKLKEAKDLIDALNLRKATIYKIGLMLLEYQYDFFKGKELRPL
CEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
KLLDLANEFNHSVSTISRAISNKYLACERGVFPIKHFFSIALDNSETSNAVIKDYLLELI
HHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHH
KNEDKKEPLSDAKILELIEEKFHLKMVRRTITKYRQLLNIASSSERKKLYLMRA
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECC
>Mature Secondary Structure 
AVLRANLSPKNKLNATLKGWLPILQSELEDLEEVLKQNALDNPLIKIENKRIKSFSDRF
CEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHC
NTKSSSDHLENLTIASKSLFETLKDQIIPPLFPTETSQKIAMDIINGLNNEGYFEENVEE
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHH
RAKILGVESEIYEKVHKRFSYLNPAGIGARDVKESFLFQLESRELDNNELYEETRKIILH
HHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
LEKHHEFSKDFYYEKALKILKSFKNPPAIEFLEKEIEVIPELFIMEVDNEIIVRLNDESY
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEEEECCEEEEEECCCCC
PTISLEEKRFKNSDYLKDKLKEAKDLIDALNLRKATIYKIGLMLLEYQYDFFKGKELRPL
CEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
KLLDLANEFNHSVSTISRAISNKYLACERGVFPIKHFFSIALDNSETSNAVIKDYLLELI
HHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHH
KNEDKKEPLSDAKILELIEEKFHLKMVRRTITKYRQLLNIASSSERKKLYLMRA
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]