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The map label for this gene is guaA

Identifier: 109947068

GI number: 109947068

Start: 414573

End: 416099

Strand: Direct

Name: guaA

Synonym: Hac_0466

Alternate gene names: 109947068

Gene position: 414573-416099 (Clockwise)

Preceding gene: 109947067

Following gene: 109947069

Centisome position: 26.68

GC content: 41.65

Gene sequence:

>1527_bases
ATGATTGTAGTATTAGATTTTGGGAGCCAATACACACAGCTGATTGCTAGAAGATTAAGAGAGAGCGGGATTTACGCTGA
AATAGTCCCCTTTTTTGAAAGCACAGAAAACATCCAAAAAAAAGCCCCTAAAGGCTTGATTTTGAGTGGGGGGCCAGCGA
GCGTGTATGCTAAAGACGCTTACACGCCCAGTGAAAAAATCTTTGATTTGAATCTACCGATTTTAGGGATTTGCTATGGC
ATGCAGTATTTGGTGGATTTTTTTGGGGGGGCAGTGGCTTGCGCGAATGAGCAAGAGTTTGGCAAGGCCGCTTTAGAGAT
CATTCAAGATTCTGTGATTTTTAAAGGCGTGAAAGCAAAAAGTCTCGTGTGGATGAGTCATATGGATAAGGTCATAACAT
TGCCTAAAGGTTTCACTACCATTGCAAAAAGCCCTAATTCGCTCCATTGTGCGATTGAAAGCGGCAAGGTTTTTGGCTTG
CAATTCCACCCAGAAGTCATTCAAAGCGAAGAGGGGGGAAAAATTTTAGAAAATTTTGCCCTTTTAGTTTGTGGCTGTGA
AAAAACTTGGGGGATGCAAAATTTCGCTCAAAAAGAAATGACGCGATTAAAAGAAAAAATTTCTGACGCTAGGGTTTTAT
GCGCGGTGAGTGGGGGCGTGGATTCTACAGTGGTCGCTACGCTATTACACAGAGCCATTAAGGATAATTTGATCGCTGTT
TTTGTCGATCATGGCTTGTTGCGTAAAAATGAAAAAGAAAAAGTCCAAGCGATGTTTAAGGATTTGCAAATCCCTTTAAA
CACCATAGACGCTAAAGAAATCTTTTTGTCTAAATTAAAGGGCGTGAGTGAGCCTGAATTGAAGCGAAAAATCATCGGCG
AGACTTTTATTGAAGTGTTTGAAAAAGAAGCCAAAAAGCACCATTTGAAAGGCAAAATTGAATTTTTAGCCCAAGGCACT
TTATACCCTGATGTGATTGAATCCGTGAGCGTTAAAGGGCCTTCAAAAGTGATAAAATCCCACCATAATGTGGGCGGACT
GCCTGAATGGATGGACTTTAAGCTCATAGAGCCTTTAAGGGAATTGTTTAAAGATGAGGTGCGTTTGTTGGGTAAAGAAC
TGGGCGTTAGCCAAGATTTTTTAATGCGCCACCCTTTTCCAGGGCCTGGACTTGCTGTAAGGATTTTGGGCGAGGTTAGT
GAGAGTAAAATCAAATGCTTACAAGAAGCGGATTTTATTTTTATAGAAGAGCTTAAAAAAGCGAATTTGTATGACAAGGT
TTGGCAAGCTTTTTGCGTGCTGTTGAACGCCCATTCTGTGGGGGTGATGGGGGATAATCGCACTTATGAAAACGCTATTT
GTTTAAGGGCGGTGGATGCGAGCGATGGCATGACGGCGAGTTTTTCGCATTTAGGGCATTCTTTTTTAGAAAAGGTTTCT
AACCGCATCACCAATGAAGTGAGCGGAATCAATAGGGTGGTGTATGATATTACTTCTAAACCACCAGGAACGATTGAATG
GGAATGA

Upstream 100 bases:

>100_bases
GAGCGTTGGTTTATGCAAAAAGTACCTGAATTAGCTGAAAGTTATAGGGTGAATTACAAAAACCAATACGACAAAAAAAC
AAAGCAATAAAGAATAAACG

Downstream 100 bases:

>100_bases
TTATCCGCAAAATTAGAGGTAAAAAGTGGGATTTTTTGGGTAAGATTAGAAACTGATTTTAAAAAAGAAAGGAAGTTAAT
GAAAAAAGGTAGTTTGGCGT

Product: GMP synthase

Products: NA

Alternate protein names: GMP synthetase; Glutamine amidotransferase

Number of amino acids: Translated: 508; Mature: 508

Protein sequence:

>508_residues
MIVVLDFGSQYTQLIARRLRESGIYAEIVPFFESTENIQKKAPKGLILSGGPASVYAKDAYTPSEKIFDLNLPILGICYG
MQYLVDFFGGAVACANEQEFGKAALEIIQDSVIFKGVKAKSLVWMSHMDKVITLPKGFTTIAKSPNSLHCAIESGKVFGL
QFHPEVIQSEEGGKILENFALLVCGCEKTWGMQNFAQKEMTRLKEKISDARVLCAVSGGVDSTVVATLLHRAIKDNLIAV
FVDHGLLRKNEKEKVQAMFKDLQIPLNTIDAKEIFLSKLKGVSEPELKRKIIGETFIEVFEKEAKKHHLKGKIEFLAQGT
LYPDVIESVSVKGPSKVIKSHHNVGGLPEWMDFKLIEPLRELFKDEVRLLGKELGVSQDFLMRHPFPGPGLAVRILGEVS
ESKIKCLQEADFIFIEELKKANLYDKVWQAFCVLLNAHSVGVMGDNRTYENAICLRAVDASDGMTASFSHLGHSFLEKVS
NRITNEVSGINRVVYDITSKPPGTIEWE

Sequences:

>Translated_508_residues
MIVVLDFGSQYTQLIARRLRESGIYAEIVPFFESTENIQKKAPKGLILSGGPASVYAKDAYTPSEKIFDLNLPILGICYG
MQYLVDFFGGAVACANEQEFGKAALEIIQDSVIFKGVKAKSLVWMSHMDKVITLPKGFTTIAKSPNSLHCAIESGKVFGL
QFHPEVIQSEEGGKILENFALLVCGCEKTWGMQNFAQKEMTRLKEKISDARVLCAVSGGVDSTVVATLLHRAIKDNLIAV
FVDHGLLRKNEKEKVQAMFKDLQIPLNTIDAKEIFLSKLKGVSEPELKRKIIGETFIEVFEKEAKKHHLKGKIEFLAQGT
LYPDVIESVSVKGPSKVIKSHHNVGGLPEWMDFKLIEPLRELFKDEVRLLGKELGVSQDFLMRHPFPGPGLAVRILGEVS
ESKIKCLQEADFIFIEELKKANLYDKVWQAFCVLLNAHSVGVMGDNRTYENAICLRAVDASDGMTASFSHLGHSFLEKVS
NRITNEVSGINRVVYDITSKPPGTIEWE
>Mature_508_residues
MIVVLDFGSQYTQLIARRLRESGIYAEIVPFFESTENIQKKAPKGLILSGGPASVYAKDAYTPSEKIFDLNLPILGICYG
MQYLVDFFGGAVACANEQEFGKAALEIIQDSVIFKGVKAKSLVWMSHMDKVITLPKGFTTIAKSPNSLHCAIESGKVFGL
QFHPEVIQSEEGGKILENFALLVCGCEKTWGMQNFAQKEMTRLKEKISDARVLCAVSGGVDSTVVATLLHRAIKDNLIAV
FVDHGLLRKNEKEKVQAMFKDLQIPLNTIDAKEIFLSKLKGVSEPELKRKIIGETFIEVFEKEAKKHHLKGKIEFLAQGT
LYPDVIESVSVKGPSKVIKSHHNVGGLPEWMDFKLIEPLRELFKDEVRLLGKELGVSQDFLMRHPFPGPGLAVRILGEVS
ESKIKCLQEADFIFIEELKKANLYDKVWQAFCVLLNAHSVGVMGDNRTYENAICLRAVDASDGMTASFSHLGHSFLEKVS
NRITNEVSGINRVVYDITSKPPGTIEWE

Specific function: Catalyzes the synthesis of GMP from XMP

COG id: COG0519

COG function: function code F; GMP synthase, PP-ATPase domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GMP-binding domain

Homologues:

Organism=Homo sapiens, GI4504035, Length=545, Percent_Identity=35.5963302752294, Blast_Score=299, Evalue=3e-81,
Organism=Escherichia coli, GI1788854, Length=519, Percent_Identity=50.6743737957611, Blast_Score=514, Evalue=1e-147,
Organism=Escherichia coli, GI1787517, Length=184, Percent_Identity=31.5217391304348, Blast_Score=67, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI133901714, Length=564, Percent_Identity=33.1560283687943, Blast_Score=273, Evalue=2e-73,
Organism=Caenorhabditis elegans, GI71992717, Length=564, Percent_Identity=33.1560283687943, Blast_Score=271, Evalue=4e-73,
Organism=Caenorhabditis elegans, GI71992710, Length=564, Percent_Identity=33.1560283687943, Blast_Score=271, Evalue=4e-73,
Organism=Saccharomyces cerevisiae, GI6323873, Length=514, Percent_Identity=55.8365758754864, Blast_Score=552, Evalue=1e-158,
Organism=Drosophila melanogaster, GI281365319, Length=546, Percent_Identity=35.1648351648352, Blast_Score=268, Evalue=6e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GUAA_HELAH (Q17YH9)

Other databases:

- EMBL:   AM260522
- RefSeq:   YP_664296.1
- ProteinModelPortal:   Q17YH9
- SMR:   Q17YH9
- STRING:   Q17YH9
- GeneID:   4177275
- GenomeReviews:   AM260522_GR
- KEGG:   hac:Hac_0466
- NMPDR:   fig|382638.8.peg.455
- eggNOG:   COG0519
- HOGENOM:   HBG391775
- OMA:   YENAICL
- PhylomeDB:   Q17YH9
- ProtClustDB:   PRK00074
- BioCyc:   HACI382638:HAC_0466-MONOMER
- HAMAP:   MF_00344
- InterPro:   IPR006220
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR001674
- InterPro:   IPR004739
- InterPro:   IPR022955
- InterPro:   IPR022310
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR00097
- PRINTS:   PR00096
- TIGRFAMs:   TIGR00884
- TIGRFAMs:   TIGR00888

Pfam domain/function: PF00117 GATase; PF00958 GMP_synt_C; PF02540 NAD_synthase

EC number: =6.3.5.2

Molecular weight: Translated: 56512; Mature: 56512

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I ; PS00639 THIOL_PROTEASE_HIS

Important sites: ACT_SITE 78-78 ACT_SITE 163-163 ACT_SITE 165-165

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVVLDFGSQYTQLIARRLRESGIYAEIVPFFESTENIQKKAPKGLILSGGPASVYAKDA
CEEEEECCCHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHCCCCEEEECCCCCEEECCC
YTPSEKIFDLNLPILGICYGMQYLVDFFGGAVACANEQEFGKAALEIIQDSVIFKGVKAK
CCCCCCEEECCCCHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHCCCCHH
SLVWMSHMDKVITLPKGFTTIAKSPNSLHCAIESGKVFGLQFHPEVIQSEEGGKILENFA
HHHHHHHCCHHEECCCCCHHHHCCCCCEEEEEECCCEEEEEECHHHHCCCCCCHHHHHHE
LLVCGCEKTWGMQNFAQKEMTRLKEKISDARVLCAVSGGVDSTVVATLLHRAIKDNLIAV
EEEECCCHHCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHCCEEEE
FVDHGLLRKNEKEKVQAMFKDLQIPLNTIDAKEIFLSKLKGVSEPELKRKIIGETFIEVF
EECCHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
EKEAKKHHLKGKIEFLAQGTLYPDVIESVSVKGPSKVIKSHHNVGGLPEWMDFKLIEPLR
HHHHHHHHCCCEEEEEECCCCCHHHHHHCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHH
ELFKDEVRLLGKELGVSQDFLMRHPFPGPGLAVRILGEVSESKIKCLQEADFIFIEELKK
HHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHCCHHHHHHHHH
ANLYDKVWQAFCVLLNAHSVGVMGDNRTYENAICLRAVDASDGMTASFSHLGHSFLEKVS
CCHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHH
NRITNEVSGINRVVYDITSKPPGTIEWE
HHHHHHHHHHHHEEEEECCCCCCCCCCC
>Mature Secondary Structure
MIVVLDFGSQYTQLIARRLRESGIYAEIVPFFESTENIQKKAPKGLILSGGPASVYAKDA
CEEEEECCCHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHCCCCEEEECCCCCEEECCC
YTPSEKIFDLNLPILGICYGMQYLVDFFGGAVACANEQEFGKAALEIIQDSVIFKGVKAK
CCCCCCEEECCCCHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHCCCCHH
SLVWMSHMDKVITLPKGFTTIAKSPNSLHCAIESGKVFGLQFHPEVIQSEEGGKILENFA
HHHHHHHCCHHEECCCCCHHHHCCCCCEEEEEECCCEEEEEECHHHHCCCCCCHHHHHHE
LLVCGCEKTWGMQNFAQKEMTRLKEKISDARVLCAVSGGVDSTVVATLLHRAIKDNLIAV
EEEECCCHHCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHCCEEEE
FVDHGLLRKNEKEKVQAMFKDLQIPLNTIDAKEIFLSKLKGVSEPELKRKIIGETFIEVF
EECCHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
EKEAKKHHLKGKIEFLAQGTLYPDVIESVSVKGPSKVIKSHHNVGGLPEWMDFKLIEPLR
HHHHHHHHCCCEEEEEECCCCCHHHHHHCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHH
ELFKDEVRLLGKELGVSQDFLMRHPFPGPGLAVRILGEVSESKIKCLQEADFIFIEELKK
HHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHCCHHHHHHHHH
ANLYDKVWQAFCVLLNAHSVGVMGDNRTYENAICLRAVDASDGMTASFSHLGHSFLEKVS
CCHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHH
NRITNEVSGINRVVYDITSKPPGTIEWE
HHHHHHHHHHHHEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA