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The map label for this gene is rrmA [H]

Identifier: 108812535

GI number: 108812535

Start: 2658264

End: 2659103

Strand: Reverse

Name: rrmA [H]

Synonym: YPN_2374

Alternate gene names: 108812535

Gene position: 2659103-2658264 (Counterclockwise)

Preceding gene: 108812537

Following gene: 108812531

Centisome position: 58.64

GC content: 48.57

Gene sequence:

>840_bases
ATGTCTTATCAGTGTCCTCTCTGTCATCAAGCACTGCAGCTTAGCCAACAGCAGTGGTGTTGTAGCAATAATCATCAGTT
TGATTGCGCCAAAGAGGGCTATGTCAATTTAATGCCAGTACAGCATAAAGGCTCCAAACAACCCGGCGATAGCCCAGAGA
TGATGCAAGCACGCCGGGCTTTTCTTGACGCTGGCTATTATCAGCCTTTGCAACAACGGGTAAGCGAGATTCTGGATGAG
GCTTTGCCGCTTGATGCAACCAGGTTATTGGACATCGGTTGCGGTGAAGGTTATTACACGGCGGCGGTGGCTGATCGGCT
AAATAAGTTGCGCCAAATGGCTATTTTTGGTCTGGATGTGGCAAAAGTCGCGGTAAGATATGGGGCTAAACGCTACCATC
AGGTCAATTTCTGTGTGGCTTCCAGCCATCGTTTACCTTTTGCCAATGGTGCACTGGATGCCGTATTACGCATTTATGCG
CCTTGTAAGGCGGTGGAACTGGCCCGCACGGTAAAGCCTGGCGGGATCGTGGTTACCGTGGCACCCGGCCCTCGCCATCT
CTATCAGTTAAAAGCACTTATTTATGCTCAGGTGCAACTGCATGATGATACAGAAGAACATCTTGATGGTTTTGAGTTAA
TCCGCCGCGAAACATTGGCCTATGACATGAAACTCACGGGTGAGCAGGGTTTTAATTTATTACAGATGACCCCGTTTGCC
TGGCGAGCATCGGTTGATACTGGGCAGAAATTGGCCGCTCAACCGTCTTTTAGTTGCGAGACTGATTTCGTCATTTCACT
GCATCGTCGTAAAACAGACAATCCTCAGAACGATATTTAA

Upstream 100 bases:

>100_bases
GGACGGCCACTGACTTGCAACCGGGGGTGTGCTTCTTTAGACTAACGCCGCATCTGCCCGCTATCTTGGCGGTTATTTAT
CTGTTATCTGGAATTCAACC

Downstream 100 bases:

>100_bases
TGTTCTGGTCCATTCTCTGGGACTATTCGCTTTATATCCCCTGTAGTTGCAGGGGAGTATTGCTGAGTAAATTAAATTTT
AATTATAATCCACTGAAATA

Product: 23S rRNA methyltransferase A

Products: NA

Alternate protein names: 23S rRNA m1G745 methyltransferase; rRNA (guanine-N(1)-)-methyltransferase [H]

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MSYQCPLCHQALQLSQQQWCCSNNHQFDCAKEGYVNLMPVQHKGSKQPGDSPEMMQARRAFLDAGYYQPLQQRVSEILDE
ALPLDATRLLDIGCGEGYYTAAVADRLNKLRQMAIFGLDVAKVAVRYGAKRYHQVNFCVASSHRLPFANGALDAVLRIYA
PCKAVELARTVKPGGIVVTVAPGPRHLYQLKALIYAQVQLHDDTEEHLDGFELIRRETLAYDMKLTGEQGFNLLQMTPFA
WRASVDTGQKLAAQPSFSCETDFVISLHRRKTDNPQNDI

Sequences:

>Translated_279_residues
MSYQCPLCHQALQLSQQQWCCSNNHQFDCAKEGYVNLMPVQHKGSKQPGDSPEMMQARRAFLDAGYYQPLQQRVSEILDE
ALPLDATRLLDIGCGEGYYTAAVADRLNKLRQMAIFGLDVAKVAVRYGAKRYHQVNFCVASSHRLPFANGALDAVLRIYA
PCKAVELARTVKPGGIVVTVAPGPRHLYQLKALIYAQVQLHDDTEEHLDGFELIRRETLAYDMKLTGEQGFNLLQMTPFA
WRASVDTGQKLAAQPSFSCETDFVISLHRRKTDNPQNDI
>Mature_278_residues
SYQCPLCHQALQLSQQQWCCSNNHQFDCAKEGYVNLMPVQHKGSKQPGDSPEMMQARRAFLDAGYYQPLQQRVSEILDEA
LPLDATRLLDIGCGEGYYTAAVADRLNKLRQMAIFGLDVAKVAVRYGAKRYHQVNFCVASSHRLPFANGALDAVLRIYAP
CKAVELARTVKPGGIVVTVAPGPRHLYQLKALIYAQVQLHDDTEEHLDGFELIRRETLAYDMKLTGEQGFNLLQMTPFAW
RASVDTGQKLAAQPSFSCETDFVISLHRRKTDNPQNDI

Specific function: Specifically methylates the guanosine in position 745 of 23S rRNA [H]

COG id: COG0500

COG function: function code QR; SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmA family [H]

Homologues:

Organism=Escherichia coli, GI1788125, Length=270, Percent_Identity=60, Blast_Score=332, Evalue=2e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013216
- InterPro:   IPR016718 [H]

Pfam domain/function: PF08241 Methyltransf_11 [H]

EC number: =2.1.1.187 [H]

Molecular weight: Translated: 31320; Mature: 31189

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYQCPLCHQALQLSQQQWCCSNNHQFDCAKEGYVNLMPVQHKGSKQPGDSPEMMQARRA
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHH
FLDAGYYQPLQQRVSEILDEALPLDATRLLDIGCGEGYYTAAVADRLNKLRQMAIFGLDV
HHHCCCHHHHHHHHHHHHHHHCCCCHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AKVAVRYGAKRYHQVNFCVASSHRLPFANGALDAVLRIYAPCKAVELARTVKPGGIVVTV
HHHHHHHHHHHHEEEEEEEECCCCCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEE
APGPRHLYQLKALIYAQVQLHDDTEEHLDGFELIRRETLAYDMKLTGEQGFNLLQMTPFA
CCCHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHHHEEEECCCCCCCEEEECCCC
WRASVDTGQKLAAQPSFSCETDFVISLHRRKTDNPQNDI
EECCCCCCCHHHCCCCCCCCCCCEEEEECCCCCCCCCCC
>Mature Secondary Structure 
SYQCPLCHQALQLSQQQWCCSNNHQFDCAKEGYVNLMPVQHKGSKQPGDSPEMMQARRA
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHH
FLDAGYYQPLQQRVSEILDEALPLDATRLLDIGCGEGYYTAAVADRLNKLRQMAIFGLDV
HHHCCCHHHHHHHHHHHHHHHCCCCHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AKVAVRYGAKRYHQVNFCVASSHRLPFANGALDAVLRIYAPCKAVELARTVKPGGIVVTV
HHHHHHHHHHHHEEEEEEEECCCCCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEE
APGPRHLYQLKALIYAQVQLHDDTEEHLDGFELIRRETLAYDMKLTGEQGFNLLQMTPFA
CCCHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHHHEEEECCCCCCCEEEECCCC
WRASVDTGQKLAAQPSFSCETDFVISLHRRKTDNPQNDI
EECCCCCCCHHHCCCCCCCCCCCEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7984109; 9097040; 9278503; 9440525 [H]