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The map label for this gene is fliA [H]

Identifier: 108812441

GI number: 108812441

Start: 2563115

End: 2563837

Strand: Reverse

Name: fliA [H]

Synonym: YPN_2280

Alternate gene names: 108812441

Gene position: 2563837-2563115 (Counterclockwise)

Preceding gene: 108812442

Following gene: 108812440

Centisome position: 56.54

GC content: 52.97

Gene sequence:

>723_bases
GTGAGTGATTTGTATACCGCCGAAGGCGTGATGGATAAGAATTCCCTCTGGAAACGCTATGTTCCTTTGGTTCGCCATGA
AGCATTGCGTCTACAGGTGCGTCTACCCGCCAGCGTGGAGTTGGACGATTTATTGCAGGCTGGGGGGATTGACCTGCTGA
ATGCTGTCGAGCGCTATGATGCTTTACAAGGCACCGCGTTTACTACGTATGCGGTACAGCGCATACGGGGGGCGATGTTG
GATGAACTGCGTAGCCGTGATTGGGCGCCTCGCAGTGTGCGCCGTAATGCACGGGAAGTGGCCAGTGCCATGCAGCAAGT
CGAACAGCGAGTAGGGCGTCCGGCGACCGAACAGGAAGTCGCGCAGGTTCTTGATATTGGCTTGGTTGAGTACCGCCAGA
TCCTGTTGGACACCAACAATAGCCAACTTTTCTCCTACGACGAATGGCGTGAAGAGCACGGCGAAAGTGTCGAGCCCCTG
TTAGAAGGGCATGAAGATGCCAATCCACTAAAACACTTGTTGGAAGGTAATCTGCGTCAGCGCGTGATTGACGCGATAGA
GGCGTTACCAGAACGCGAAAAAATGGTGCTTACGTTGTATTACCAGGAAGAGCTGAATTTGAAAGAAATTGGTGCGGTGC
TTGAAGTTGGGGAGTCCCGCGTCAGTCAACTTCACAGCCAAGCGATAAAACGCTTACGCGCCCGCCTAAATAACGACGCT
TAA

Upstream 100 bases:

>100_bases
ATAAGCCAAATAGCCGCCCTTTTATAGGTCTATTCCGGCGATTGGCTGACTTACCTGACAGGATAATGGAACGACTCTCT
TACCCTTAGGTGCCTGTCTC

Downstream 100 bases:

>100_bases
TTCACGTTCAATCTGCTCCCTGCGGGGTAGTCACCCGGATGACTGACGCGTAGTTAGCGTTTCATTGGGATGATGAGCCT
ATTCAGCGGCTCACCCTGCG

Product: flagellar biosynthesis sigma factor

Products: NA

Alternate protein names: Sigma-27; Sigma-28; Sigma-F factor [H]

Number of amino acids: Translated: 240; Mature: 239

Protein sequence:

>240_residues
MSDLYTAEGVMDKNSLWKRYVPLVRHEALRLQVRLPASVELDDLLQAGGIDLLNAVERYDALQGTAFTTYAVQRIRGAML
DELRSRDWAPRSVRRNAREVASAMQQVEQRVGRPATEQEVAQVLDIGLVEYRQILLDTNNSQLFSYDEWREEHGESVEPL
LEGHEDANPLKHLLEGNLRQRVIDAIEALPEREKMVLTLYYQEELNLKEIGAVLEVGESRVSQLHSQAIKRLRARLNNDA

Sequences:

>Translated_240_residues
MSDLYTAEGVMDKNSLWKRYVPLVRHEALRLQVRLPASVELDDLLQAGGIDLLNAVERYDALQGTAFTTYAVQRIRGAML
DELRSRDWAPRSVRRNAREVASAMQQVEQRVGRPATEQEVAQVLDIGLVEYRQILLDTNNSQLFSYDEWREEHGESVEPL
LEGHEDANPLKHLLEGNLRQRVIDAIEALPEREKMVLTLYYQEELNLKEIGAVLEVGESRVSQLHSQAIKRLRARLNNDA
>Mature_239_residues
SDLYTAEGVMDKNSLWKRYVPLVRHEALRLQVRLPASVELDDLLQAGGIDLLNAVERYDALQGTAFTTYAVQRIRGAMLD
ELRSRDWAPRSVRRNAREVASAMQQVEQRVGRPATEQEVAQVLDIGLVEYRQILLDTNNSQLFSYDEWREEHGESVEPLL
EGHEDANPLKHLLEGNLRQRVIDAIEALPEREKMVLTLYYQEELNLKEIGAVLEVGESRVSQLHSQAIKRLRARLNNDA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This alternative sigma factor is specific for the flagellar operon [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=238, Percent_Identity=81.5126050420168, Blast_Score=392, Evalue=1e-110,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR012845
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 27483; Mature: 27352

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLYTAEGVMDKNSLWKRYVPLVRHEALRLQVRLPASVELDDLLQAGGIDLLNAVERYD
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHCCHHHHHHHHHHH
ALQGTAFTTYAVQRIRGAMLDELRSRDWAPRSVRRNAREVASAMQQVEQRVGRPATEQEV
HHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
AQVLDIGLVEYRQILLDTNNSQLFSYDEWREEHGESVEPLLEGHEDANPLKHLLEGNLRQ
HHHHHHHHHHHHHHHHCCCCCCEECHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHCHHHH
RVIDAIEALPEREKMVLTLYYQEELNLKEIGAVLEVGESRVSQLHSQAIKRLRARLNNDA
HHHHHHHHCCCCCCEEEEEEECHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SDLYTAEGVMDKNSLWKRYVPLVRHEALRLQVRLPASVELDDLLQAGGIDLLNAVERYD
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHCCHHHHHHHHHHH
ALQGTAFTTYAVQRIRGAMLDELRSRDWAPRSVRRNAREVASAMQQVEQRVGRPATEQEV
HHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
AQVLDIGLVEYRQILLDTNNSQLFSYDEWREEHGESVEPLLEGHEDANPLKHLLEGNLRQ
HHHHHHHHHHHHHHHHCCCCCCEECHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHCHHHH
RVIDAIEALPEREKMVLTLYYQEELNLKEIGAVLEVGESRVSQLHSQAIKRLRARLNNDA
HHHHHHHHCCCCCCEEEEEEECHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730257 [H]