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The map label for this gene is truA [H]

Identifier: 108812343

GI number: 108812343

Start: 2465120

End: 2466001

Strand: Reverse

Name: truA [H]

Synonym: YPN_2181

Alternate gene names: 108812343

Gene position: 2466001-2465120 (Counterclockwise)

Preceding gene: 108812344

Following gene: 108812342

Centisome position: 54.38

GC content: 50.91

Gene sequence:

>882_bases
ATGTCCGGGTTTGAATTAGATCAGGAAGAAACATTAGTTCAGCAAAACACATTGGCTCAGCAAGAAAAACCGGCAGAAGT
GGCCGGTTTGAAAATTGCGTTGGGTATTGAGTATAACGGTAGCCGCTATTTTGGTTGGCAACGGCAGCAAGAGGTTGCCA
GCGTGCAGGCCTGTCTTGAGGCGGCATTATCCAAAGTGGCTAATGAACCCATTGGGGTCTTCTGCGCTGGCCGTACCGAT
GCAGGGGTCCATGCTACCGGGCAAGTTGTGCACTTTGTTACCTCTGCAGTACGTAAAGACGCCGCCTGGACTATGGGGGT
CAACAGCCATTTGCCCGCGGATATCGCAGTGCGTTGGGTCAAAACCGTCGATAACGATTTTCATGCCCGCTTTAGCGCAA
CGGCCCGCCGCTATCGCTATATCATTTTTAGCCACCGCTATCGCCCTGCGATATTGGCGCAAGGGGTCACCCATTGCTAT
ATGCCATTGGATGCAGAAAAAATGGAACGCGCCGCGCAATGTTTACTCGGCGAAAATGATTTTACCTCTTTCCGTGCCGT
ACAATGTCAATCCCGCACGCCATGGAGAAATGTAAAACATGTCAAAGTTACCCGACATGGCGCGTATATCGTGGTAGATA
TCAAGGCCAACGCGTTTGTACATCATATGGTGCGCAATATCGTTGGGAGCCTGATAGAAATTGGTTGTGGCAATCAGGAC
GTGACCTGGATGGCAGAATTGTTGGCGTTGAAGGACAGAACACGTGCTGCCGCAACCGCCAAAGCCGATGGGTTGTATTT
AGTGTCTGTCGATTACCCGGACCACTTCGCGTTACCCAAAGTTCCTATGGGGCCTTTGTTTTTGGCGGATGACGAGGGTT
AA

Upstream 100 bases:

>100_bases
AAGTGCTGCAATTCTGGTCGGTGGCTGACAACATTCGTTTTGGTGGCGCATTGATGGCGATTCAAACGGCAGAGCGCCTG
TTACAGGAGCAACTATACTG

Downstream 100 bases:

>100_bases
CGGTTCACCGGTGACTAAAGATACCGAGAGCCAATTGTCAGGTATGTCGATGGCAAACTGAGGCGTCGCTTTGGGCTAAG
GAATACCTAAGGAAATTCAG

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MSGFELDQEETLVQQNTLAQQEKPAEVAGLKIALGIEYNGSRYFGWQRQQEVASVQACLEAALSKVANEPIGVFCAGRTD
AGVHATGQVVHFVTSAVRKDAAWTMGVNSHLPADIAVRWVKTVDNDFHARFSATARRYRYIIFSHRYRPAILAQGVTHCY
MPLDAEKMERAAQCLLGENDFTSFRAVQCQSRTPWRNVKHVKVTRHGAYIVVDIKANAFVHHMVRNIVGSLIEIGCGNQD
VTWMAELLALKDRTRAAATAKADGLYLVSVDYPDHFALPKVPMGPLFLADDEG

Sequences:

>Translated_293_residues
MSGFELDQEETLVQQNTLAQQEKPAEVAGLKIALGIEYNGSRYFGWQRQQEVASVQACLEAALSKVANEPIGVFCAGRTD
AGVHATGQVVHFVTSAVRKDAAWTMGVNSHLPADIAVRWVKTVDNDFHARFSATARRYRYIIFSHRYRPAILAQGVTHCY
MPLDAEKMERAAQCLLGENDFTSFRAVQCQSRTPWRNVKHVKVTRHGAYIVVDIKANAFVHHMVRNIVGSLIEIGCGNQD
VTWMAELLALKDRTRAAATAKADGLYLVSVDYPDHFALPKVPMGPLFLADDEG
>Mature_292_residues
SGFELDQEETLVQQNTLAQQEKPAEVAGLKIALGIEYNGSRYFGWQRQQEVASVQACLEAALSKVANEPIGVFCAGRTDA
GVHATGQVVHFVTSAVRKDAAWTMGVNSHLPADIAVRWVKTVDNDFHARFSATARRYRYIIFSHRYRPAILAQGVTHCYM
PLDAEKMERAAQCLLGENDFTSFRAVQCQSRTPWRNVKHVKVTRHGAYIVVDIKANAFVHHMVRNIVGSLIEIGCGNQDV
TWMAELLALKDRTRAAATAKADGLYLVSVDYPDHFALPKVPMGPLFLADDEG

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=283, Percent_Identity=28.9752650176678, Blast_Score=79, Evalue=5e-15,
Organism=Escherichia coli, GI1788657, Length=271, Percent_Identity=78.2287822878229, Blast_Score=449, Evalue=1e-127,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 32503; Mature: 32372

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGFELDQEETLVQQNTLAQQEKPAEVAGLKIALGIEYNGSRYFGWQRQQEVASVQACLE
CCCCCCCHHHHHHHHHHHHHHCCCCHHCCEEEEEEEEECCCEEECCHHHHHHHHHHHHHH
AALSKVANEPIGVFCAGRTDAGVHATGQVVHFVTSAVRKDAAWTMGVNSHLPADIAVRWV
HHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHH
KTVDNDFHARFSATARRYRYIIFSHRYRPAILAQGVTHCYMPLDAEKMERAAQCLLGEND
HHHCCCHHHHHHHHHHHEEEEEEECCCCHHHHHCCCHHEECCCCHHHHHHHHHHHCCCCC
FTSFRAVQCQSRTPWRNVKHVKVTRHGAYIVVDIKANAFVHHMVRNIVGSLIEIGCGNQD
CCCEEEEEECCCCCCCCCEEEEEEECCEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCC
VTWMAELLALKDRTRAAATAKADGLYLVSVDYPDHFALPKVPMGPLFLADDEG
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCC
>Mature Secondary Structure 
SGFELDQEETLVQQNTLAQQEKPAEVAGLKIALGIEYNGSRYFGWQRQQEVASVQACLE
CCCCCCHHHHHHHHHHHHHHCCCCHHCCEEEEEEEEECCCEEECCHHHHHHHHHHHHHH
AALSKVANEPIGVFCAGRTDAGVHATGQVVHFVTSAVRKDAAWTMGVNSHLPADIAVRWV
HHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHH
KTVDNDFHARFSATARRYRYIIFSHRYRPAILAQGVTHCYMPLDAEKMERAAQCLLGEND
HHHCCCHHHHHHHHHHHEEEEEEECCCCHHHHHCCCHHEECCCCHHHHHHHHHHHCCCCC
FTSFRAVQCQSRTPWRNVKHVKVTRHGAYIVVDIKANAFVHHMVRNIVGSLIEIGCGNQD
CCCEEEEEECCCCCCCCCEEEEEEECCEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCC
VTWMAELLALKDRTRAAATAKADGLYLVSVDYPDHFALPKVPMGPLFLADDEG
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA