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The map label for this gene is menH [H]

Identifier: 108812283

GI number: 108812283

Start: 2396922

End: 2397740

Strand: Reverse

Name: menH [H]

Synonym: YPN_2121

Alternate gene names: 108812283

Gene position: 2397740-2396922 (Counterclockwise)

Preceding gene: 108812284

Following gene: 108812282

Centisome position: 52.88

GC content: 54.33

Gene sequence:

>819_bases
ATGACGACACTGGCTTGCCGAAAACTGGCCCCTCATCCGGAATCGCCCCGCCATCAACACGCTGGGCCTTGGTTGGTTTG
GTTACATGGTTTGTTGGGCAGCGGACAAGATTGGCTGCCCGTGGCACAACTGTGTGGCGATTATCCGTCATTGTTGATCG
ATCTACCGGGCCATGGACAATCCGTCTCCCTGAGCGCCGATGGTTTTGCCGATATCAGTCGCCAGTTGTCACAAACATTA
CAGGCTAACGGTATTCGCGAATACTGGCTGGCCGGCTACTCTTTAGGCGGTAGGATCGCGATATATCACGCCTGTTATGG
TCGCCATCATGGCTTGCAGGGGTTATTGGTTGAAGGGGGAAACCTCGGGCTGGAAAACGCTGAACTACGTCAGGCGCGGT
TGCAACAAGACCGCCAATGGGCGCAGCGCTTTCGCCAGGAGCCTCTGCCACAGGTATTGGATGACTGGTATCAGCAGGCG
GTATTTGCCGATTTAGATCCACAACAACGTGAACAACTGGTGTTATTGCGGGCCGATAACCATGGCCTGGCTGTGGCCGA
GATGCTAGAGGCAACGTCACTAGGGCATCAACCGTGGTTGTTACCGGCCCTGCAACGGCTCAACGTCCCTTATACTTATT
TGTGTGGTGATCGTGACCATAAGTTCCTGCAACTGGCACAGCAATATCGATTGCCATTACACACGCTAGCCCGCGCCGGG
CACAATGCCCATCGGGCGAATCCCGGTGCTTTTGCTGCACAGGTGCTGGCATTTTTGTCACAGTCATCATGCTTACCACC
GTCATCATTATCTCGTTAA

Upstream 100 bases:

>100_bases
GGGGAACCACGCTGATTGAAGTCCAGGTGCCACCAAGTCAGGGGGCAGAAACACTGCAACAGTTAGTACAACAGGTGACA
TTAATACCGCAGGTGGCCCC

Downstream 100 bases:

>100_bases
AGGAATGTTTACTATGCTTTATCCGAGCGAAGAACAATTGTACGCCGCCATCGACTGGCTGGATTGCTCCGCCGGATTTG
AAGATATTCGTTATCACAAA

Product: acyl-CoA thioester hydrolase YfbB

Products: NA

Alternate protein names: SHCHC synthase [H]

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MTTLACRKLAPHPESPRHQHAGPWLVWLHGLLGSGQDWLPVAQLCGDYPSLLIDLPGHGQSVSLSADGFADISRQLSQTL
QANGIREYWLAGYSLGGRIAIYHACYGRHHGLQGLLVEGGNLGLENAELRQARLQQDRQWAQRFRQEPLPQVLDDWYQQA
VFADLDPQQREQLVLLRADNHGLAVAEMLEATSLGHQPWLLPALQRLNVPYTYLCGDRDHKFLQLAQQYRLPLHTLARAG
HNAHRANPGAFAAQVLAFLSQSSCLPPSSLSR

Sequences:

>Translated_272_residues
MTTLACRKLAPHPESPRHQHAGPWLVWLHGLLGSGQDWLPVAQLCGDYPSLLIDLPGHGQSVSLSADGFADISRQLSQTL
QANGIREYWLAGYSLGGRIAIYHACYGRHHGLQGLLVEGGNLGLENAELRQARLQQDRQWAQRFRQEPLPQVLDDWYQQA
VFADLDPQQREQLVLLRADNHGLAVAEMLEATSLGHQPWLLPALQRLNVPYTYLCGDRDHKFLQLAQQYRLPLHTLARAG
HNAHRANPGAFAAQVLAFLSQSSCLPPSSLSR
>Mature_271_residues
TTLACRKLAPHPESPRHQHAGPWLVWLHGLLGSGQDWLPVAQLCGDYPSLLIDLPGHGQSVSLSADGFADISRQLSQTLQ
ANGIREYWLAGYSLGGRIAIYHACYGRHHGLQGLLVEGGNLGLENAELRQARLQQDRQWAQRFRQEPLPQVLDDWYQQAV
FADLDPQQREQLVLLRADNHGLAVAEMLEATSLGHQPWLLPALQRLNVPYTYLCGDRDHKFLQLAQQYRLPLHTLARAGH
NAHRANPGAFAAQVLAFLSQSSCLPPSSLSR

Specific function: Catalyzes a proton abstraction reaction that results in 2,5-elimination of pyruvate from 2-succinyl-5-enolpyruvyl-6- hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) and the formation of 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate (SHCHC). Is also

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. MenH family [H]

Homologues:

Organism=Escherichia coli, GI1788598, Length=250, Percent_Identity=49.2, Blast_Score=233, Evalue=9e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR022485 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =4.2.99.20 [H]

Molecular weight: Translated: 30299; Mature: 30168

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLACRKLAPHPESPRHQHAGPWLVWLHGLLGSGQDWLPVAQLCGDYPSLLIDLPGHGQ
CCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHCCCCCEEEEECCCCCC
SVSLSADGFADISRQLSQTLQANGIREYWLAGYSLGGRIAIYHACYGRHHGLQGLLVEGG
EEEECCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCCEEEEEEEHHHHCCCCCEEEEECC
NLGLENAELRQARLQQDRQWAQRFRQEPLPQVLDDWYQQAVFADLDPQQREQLVLLRADN
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHCCEEEEEECC
HGLAVAEMLEATSLGHQPWLLPALQRLNVPYTYLCGDRDHKFLQLAQQYRLPLHTLARAG
CCHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCHHHHHHCC
HNAHRANPGAFAAQVLAFLSQSSCLPPSSLSR
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TTLACRKLAPHPESPRHQHAGPWLVWLHGLLGSGQDWLPVAQLCGDYPSLLIDLPGHGQ
CCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHCCCCCEEEEECCCCCC
SVSLSADGFADISRQLSQTLQANGIREYWLAGYSLGGRIAIYHACYGRHHGLQGLLVEGG
EEEECCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCCEEEEEEEHHHHCCCCCEEEEECC
NLGLENAELRQARLQQDRQWAQRFRQEPLPQVLDDWYQQAVFADLDPQQREQLVLLRADN
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHCCEEEEEECC
HGLAVAEMLEATSLGHQPWLLPALQRLNVPYTYLCGDRDHKFLQLAQQYRLPLHTLARAG
CCHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCHHHHHHCC
HNAHRANPGAFAAQVLAFLSQSSCLPPSSLSR
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA